Increasing plant density is an effective strategy for enhancing crop yield per unit land area.A key architectural trait for crops adapting to high planting density is a smaller leaf angle(LA).Previous studies have dem...Increasing plant density is an effective strategy for enhancing crop yield per unit land area.A key architectural trait for crops adapting to high planting density is a smaller leaf angle(LA).Previous studies have demonstrated that LG1,a SQUAMOSA BINDING PROTEIN(SBP)transcription factor,plays a critical role in LA establishment.However,the molecular mechanisms underlying the regulation of LG1 on LA formation remain largely unclear.In this study,we conduct comparative RNA-seq analysis of the preligule band(PLB)region of wild type and lg1 mutant leaves.Gene Ontology(GO)term enrichment analysis reveals enrichment of phytohormone pathways and transcription factors,including three auxin transporter genes ZmPIN1a,ZmPIN1b,and ZmPIN1c.Further molecular experiments demonstrate that LG1 can directly bind to the promoter region of these auxin transporter genes and activate their transcription.We also show that double and triple mutants of these ZmPINs genes exhibit varying degrees of auricle size reduction and thus decreased LA.On the contrary,overexpression of ZmPIN1a causes larger auricle and LA.Taken together,our findings establish a functional link between LG1 and auxin transport in regulating PLB formation and provide valuable targets for genetic improvement of LA for breeding high-density tolerant maize cultivars.展开更多
基金supported by the National Key Research and Development Program of China(2021YFF1000301)the National Natural Science Foundation of China(32472179,32130077,32201835)+2 种基金the Natural Science Foundation of Hebei Province(C2022407068)the Natural Science Foundation of Guangdong Province(2024A1515030237,2022A1515011002)the Hainan Yazhou Bay Seed Lab(B21HJ8101).
文摘Increasing plant density is an effective strategy for enhancing crop yield per unit land area.A key architectural trait for crops adapting to high planting density is a smaller leaf angle(LA).Previous studies have demonstrated that LG1,a SQUAMOSA BINDING PROTEIN(SBP)transcription factor,plays a critical role in LA establishment.However,the molecular mechanisms underlying the regulation of LG1 on LA formation remain largely unclear.In this study,we conduct comparative RNA-seq analysis of the preligule band(PLB)region of wild type and lg1 mutant leaves.Gene Ontology(GO)term enrichment analysis reveals enrichment of phytohormone pathways and transcription factors,including three auxin transporter genes ZmPIN1a,ZmPIN1b,and ZmPIN1c.Further molecular experiments demonstrate that LG1 can directly bind to the promoter region of these auxin transporter genes and activate their transcription.We also show that double and triple mutants of these ZmPINs genes exhibit varying degrees of auricle size reduction and thus decreased LA.On the contrary,overexpression of ZmPIN1a causes larger auricle and LA.Taken together,our findings establish a functional link between LG1 and auxin transport in regulating PLB formation and provide valuable targets for genetic improvement of LA for breeding high-density tolerant maize cultivars.
文摘目的 建立武汉地区表观健康成人血清可溶性转铁蛋白受体(soluble transferrin receptor,sTfR)和可溶性转铁蛋白受体与血清铁蛋白常用对数的比值(sTfR/log serum ferritin index,sTfR/lgSF)参考区间,为临床对铁缺乏和缺铁性贫血诊治提供依据。方法 选取武汉亚心总医院表观健康体检者和献血屋献血者273名,检测sTfR、其他铁代谢指标以及超敏C-反应蛋白(high sensitivity C-reactive protein,hsCRP),并计算sTfR/lgSF值,采用百分位数法(P2.5~P97.5)建立sTfR和sTfR/lgSF参考区间。采用Spearman相关分析评价sTfR、sTfR/lgSF与其他铁代谢指标、hsCPR相关性。结果 sTfR水平(M,mg/L)男女(1.01 vs 1.07)间差异不具统计学意义(P>0.05),但男性sTfR/lgSF水平明显低于女性(0.45 vs 0.62),差异有统计学意义(P<0.05);各年龄组间sTfR(M,mg/L)、sTfR/lgSF(M)比较,分别为(1.07 vs 1.02 vs 1.00)、(0.52 vs 0.53 vs 0.51),差异均不具统计学意义(P>0.05);sTfR参考区间为(0.72~1.68)mg/L,根据性别建立sTfR/lgSF参考区间为男性(0.31~0.88)、女性(0.37~1.19);相关性分析结果显示:sTfR、sTfR/lgSF与hsCRP无相关性(r=0.043,P>0.05)、(r=-0.064,P>0.05),而血清铁蛋白(serum ferritin,SF)、血清铁(serum iron,SI)、转铁蛋白饱和度(transferrin saturation,TSAT)与hsCRP均有相关性(r=0.128,P<0.05)、(r=-0.195,P<0.01)、(r=-0.173,P<0.01);sTfR与SF无相关性(r=-0.115,P>0.05),而sTfR/lgSF与SF有显著性相关(r=-0.685,P<0.01)。结论 初步建立了武汉地区表观健康成人血清sTfR及sTfR/lgSF的参考区间。sTfR和sTfR/lgSF不受炎性因子影响,对在炎症作用下血清铁蛋白升高的贫血患者,鉴别是否存在铁缺乏方面有着重大意义。
