Lateral flow immunoassay(LFIA)has become popular in laboratories,at-home testing,and medical diagnostics due to its minimal cost and user-friendliness.Nevertheless,conventional test strips based on colloidal gold can ...Lateral flow immunoassay(LFIA)has become popular in laboratories,at-home testing,and medical diagnostics due to its minimal cost and user-friendliness.Nevertheless,conventional test strips based on colloidal gold can only obtain qualitative or semi-quantitative results with low sensitivity.In this work,AuFe_(3)O_(4) dumbbell-like nanoparticles were synthesized and used as the LFIA labelling marker for highly sensitive colorimetric-photothermal dual-mode detection of SARS-CoV-2 spike(S)protein.The unique dumbbell structure of Au-Fe_(3)O_(4) NPs makes it possible to combine the best features of both Au NPs and Fe_(3)O_(4) NPs.The increased surface area of these NPs enhances their LSPR effect and photothermal effect,which achieves signal amplification to increase sensitivity.The Au-Fe_(3)O_(4) NPs modified with S protein antibody could identify S protein in samples,which were recognized and accumulated on T-line by another antibody,generating color band for qualitative colorimetric detection.The T-line was irradiated by laser to obtain temperature change for quantitative detection of photothermal.In optimized conditions,the detection limit was 1.22 pg/m L,three orders of magnitude more sensitive than colorimetric detection.Finally,the approach was performed on SARS-CoV-2 pseudovirus samples and outperformed traditional colloidal gold strips.This LFIA platform exhibits significant promise for practical implementation,as it can satisfy the need for low-cost,high-sensitivity,and home-based quantitative detection for respiratory infectious diseases.展开更多
Lateral flow immunoassays(LFIAs) have been developed rapidly in recent years and used in a wide range of application at point-of-care-testing(POCT),where small biomolecules can be conveniently examined on a test strip...Lateral flow immunoassays(LFIAs) have been developed rapidly in recent years and used in a wide range of application at point-of-care-testing(POCT),where small biomolecules can be conveniently examined on a test strip.Compared with other biochemical detection methods such as ELISA(enzyme linked immunosorbent assay) or mass spectrometry method,LFIAs have the advantages of low cost,easy operation and short time-consuming.However,it suffers from low sensitivity since conventional LFIA can only realize qualitative detection based on colorimetric signals.With the increasing demand for more accurate and sensitive determination,novel nanomaterials have been used as labels in LFIAs due to their unique advantages in physical and chemical properties.Colloidal gold,fluorescent nano particles,SERSactive nanomaterials,magnetic nanoparticles and carbon nanomaterials are utilized in LFIAs to produce different kinds of signals for quantitative or semi-quantitative detection.This review paper first gives a description of the LFIA principles,and then focuses on the state-of-the-art nanomaterial labelling technology in LFIAs.At last,the conclusion and outlook are given to inspire exploration of more advanced nanomaterials for the development of future LFIAs.展开更多
The on-going global pandemic of coronavirus disease 2019(COVID-19)caused by a novel coronavirus called severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has been underway for about 11 months.Through November ...The on-going global pandemic of coronavirus disease 2019(COVID-19)caused by a novel coronavirus called severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has been underway for about 11 months.Through November 20,2020,51 detection kits for SARS-CoV-2 nucleic acids(24 kits),antibodies(25 kits),or antigens(2 kits)have been approved by the National Medical Products Administration of China(NMPA).Convenient and reliable SARS-CoV-2 detection assays are urgently needed worldwide for strategic control of the pandemic.In this review,the detection kits approved in China are summarised and the three types of tests,namely nucleic acid,serological and antigen detection,which are available for the detection of COVID-19 are discussed in detail.The development of novel detection kits will lay the foundation for the control and prevention of the COVID-19 pandemic globally.展开更多
Foodborne pathogens cause diseases in humans.The traditional methods of detecting foodborne pathogens are time-consuming.The lateral flow immunoassay(LFIA)has become a widely used detection platform for onsite testing...Foodborne pathogens cause diseases in humans.The traditional methods of detecting foodborne pathogens are time-consuming.The lateral flow immunoassay(LFIA)has become a widely used detection platform for onsite testing of various foodborne pathogens due to its time-efficiency,cost-effectiveness,portability,and ease of use.With the development of novel nanomaterials,the sensitivity of the LFIA has improved tremendously compared with traditional colorimetric LFIA sensors.This review first summarizes the principles and corresponding formats of the LFIA.Then,a detailed classification of nanomaterial label(e.g.,metallic,carbon and selenium,fluorescent,and magnetic nanoparticles)synthesis,signal amplification strategy,and detection principles are discussed as related to food safety.Subsequently,the LFIA used in the detection of pathogenic bacteria,including Escherichia coli,Vibrio parahaemolyticus,Staphylococcus aureus,Listeria monocytogenes,and Salmonella,are classified and summarized.Multiple signal modes have been explored that improve the sensitivity of foodborne pathogen detection.Further improvement should focus on the design and preparation of high signal-to-noise ratio nanomaterials to achieve highly sensitive detection,and multitarget and multimode sensing.展开更多
Wheat gliadin is one of the most important causes of gluten sensitivity.This study aimed to develop a sandwich enzyme-linked immunosorbent assay(ELISA)and gold nanoparticle-based lateral flow(LFIA)strips to detect whe...Wheat gliadin is one of the most important causes of gluten sensitivity.This study aimed to develop a sandwich enzyme-linked immunosorbent assay(ELISA)and gold nanoparticle-based lateral flow(LFIA)strips to detect wheat allergen in food.Through cell screening,ten monoclonal antibodies(mAbs)against gliadin and after optimization were obtained,a pair of mAbs suitable for its detection(capture antibody:mAb 7;detection antibody:HRP-labeled mAb 6)were identified.Based on two of these antibodies,a sandwich ELISA with limit of detection(LOD)of 60 ng/mL in negative milk,and more importantly,negligible cross-reactivity to other allergens was developed.The average recoveries for gliadin in negative milk were 99.16%-100.07%using the sandwich ELISA.We also developed LFIA strips for the rapid detection of gliadin with visual limit of detection(vLOD)of 25 ng/mL and calculated LOD value of 6.56 ng/mL in negative milk.The test results of positive samples obtained from the LFIA strips were highly consistent with those of the sandwich ELISA.Thus,the developed LFIA strip is an effective and reliable tool for the rapid and on-site detection of wheat allergen in milk.展开更多
Point-of-care testing(POCT)is essential for providing a rapid diagnostic result in a prompt on-site diagnosis and treatment.A quick analysis time and a high sensitivity,with a sample-to-answer format,are the most impo...Point-of-care testing(POCT)is essential for providing a rapid diagnostic result in a prompt on-site diagnosis and treatment.A quick analysis time and a high sensitivity,with a sample-to-answer format,are the most important features for current POCT diagnostic systems.This review covers recent advances in POCT technologies with an emphasis on demonstrated and commercially available POCT diagnostic systems with laboratory quality using lateral flow immunoassay(LFIA).The system includes the integration of nanoparticles(NPs)in lateral flow test strips(LFTSs)and the mechanism through which particles improve the analytical performance of the fabricated strips.Several examples of NP-based LFTSs were selected to illustrate novel concepts or devices with promising applications as screening tools and superior alternatives to existing conventional strategies in clinical analysis,food safety,and environmental monitoring.In each analyte category,detection methods,configuration of LFIA modules,and advantages of POCT systems are reviewed and discussed along with future prospects.This review also discusses novel signal-enhancement strategies,optimal reader systems,and multiplex design prototypes,which have been employed for highly sensitive multiplex assay of LFTSs.展开更多
基金supported by the Natural Scientific Foundation of Shandong(Nos.ZR2023MC039,ZR2022JQ07)the National Natural Science Foundation of China(No.21876206)+1 种基金the Fundamental Research Funds for the Central Universities(No.21CX06014A)the Taishan Scholarship of Shandong Province(No.tsqn202211080)。
文摘Lateral flow immunoassay(LFIA)has become popular in laboratories,at-home testing,and medical diagnostics due to its minimal cost and user-friendliness.Nevertheless,conventional test strips based on colloidal gold can only obtain qualitative or semi-quantitative results with low sensitivity.In this work,AuFe_(3)O_(4) dumbbell-like nanoparticles were synthesized and used as the LFIA labelling marker for highly sensitive colorimetric-photothermal dual-mode detection of SARS-CoV-2 spike(S)protein.The unique dumbbell structure of Au-Fe_(3)O_(4) NPs makes it possible to combine the best features of both Au NPs and Fe_(3)O_(4) NPs.The increased surface area of these NPs enhances their LSPR effect and photothermal effect,which achieves signal amplification to increase sensitivity.The Au-Fe_(3)O_(4) NPs modified with S protein antibody could identify S protein in samples,which were recognized and accumulated on T-line by another antibody,generating color band for qualitative colorimetric detection.The T-line was irradiated by laser to obtain temperature change for quantitative detection of photothermal.In optimized conditions,the detection limit was 1.22 pg/m L,three orders of magnitude more sensitive than colorimetric detection.Finally,the approach was performed on SARS-CoV-2 pseudovirus samples and outperformed traditional colloidal gold strips.This LFIA platform exhibits significant promise for practical implementation,as it can satisfy the need for low-cost,high-sensitivity,and home-based quantitative detection for respiratory infectious diseases.
基金financial support from the National Natural Science Foundation of China(51802060)Shenzhen Science and Technology Program(Grant No.:KQTD20170809110344233)+1 种基金Shenzhen Bay Laboratory(SZBL2019062801005)Natural Science Foundation of Guangdong Province(No.2019A1515010762)。
文摘Lateral flow immunoassays(LFIAs) have been developed rapidly in recent years and used in a wide range of application at point-of-care-testing(POCT),where small biomolecules can be conveniently examined on a test strip.Compared with other biochemical detection methods such as ELISA(enzyme linked immunosorbent assay) or mass spectrometry method,LFIAs have the advantages of low cost,easy operation and short time-consuming.However,it suffers from low sensitivity since conventional LFIA can only realize qualitative detection based on colorimetric signals.With the increasing demand for more accurate and sensitive determination,novel nanomaterials have been used as labels in LFIAs due to their unique advantages in physical and chemical properties.Colloidal gold,fluorescent nano particles,SERSactive nanomaterials,magnetic nanoparticles and carbon nanomaterials are utilized in LFIAs to produce different kinds of signals for quantitative or semi-quantitative detection.This review paper first gives a description of the LFIA principles,and then focuses on the state-of-the-art nanomaterial labelling technology in LFIAs.At last,the conclusion and outlook are given to inspire exploration of more advanced nanomaterials for the development of future LFIAs.
基金supported by the National Key Program for Infectious Disease of China(2018ZX10101002)the National Key Research and Development Program of China(2016YFD0500301,2020YFC0840900)。
文摘The on-going global pandemic of coronavirus disease 2019(COVID-19)caused by a novel coronavirus called severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has been underway for about 11 months.Through November 20,2020,51 detection kits for SARS-CoV-2 nucleic acids(24 kits),antibodies(25 kits),or antigens(2 kits)have been approved by the National Medical Products Administration of China(NMPA).Convenient and reliable SARS-CoV-2 detection assays are urgently needed worldwide for strategic control of the pandemic.In this review,the detection kits approved in China are summarised and the three types of tests,namely nucleic acid,serological and antigen detection,which are available for the detection of COVID-19 are discussed in detail.The development of novel detection kits will lay the foundation for the control and prevention of the COVID-19 pandemic globally.
基金This work is financially supported by the National Natural Science Foundation of China(22236002)Science and Technology Project of Jiangsu Market Supervision Administration(SZSJ2022001).
文摘Foodborne pathogens cause diseases in humans.The traditional methods of detecting foodborne pathogens are time-consuming.The lateral flow immunoassay(LFIA)has become a widely used detection platform for onsite testing of various foodborne pathogens due to its time-efficiency,cost-effectiveness,portability,and ease of use.With the development of novel nanomaterials,the sensitivity of the LFIA has improved tremendously compared with traditional colorimetric LFIA sensors.This review first summarizes the principles and corresponding formats of the LFIA.Then,a detailed classification of nanomaterial label(e.g.,metallic,carbon and selenium,fluorescent,and magnetic nanoparticles)synthesis,signal amplification strategy,and detection principles are discussed as related to food safety.Subsequently,the LFIA used in the detection of pathogenic bacteria,including Escherichia coli,Vibrio parahaemolyticus,Staphylococcus aureus,Listeria monocytogenes,and Salmonella,are classified and summarized.Multiple signal modes have been explored that improve the sensitivity of foodborne pathogen detection.Further improvement should focus on the design and preparation of high signal-to-noise ratio nanomaterials to achieve highly sensitive detection,and multitarget and multimode sensing.
基金supported by National Key R&D Program of China(2019YFC1605004).
文摘Wheat gliadin is one of the most important causes of gluten sensitivity.This study aimed to develop a sandwich enzyme-linked immunosorbent assay(ELISA)and gold nanoparticle-based lateral flow(LFIA)strips to detect wheat allergen in food.Through cell screening,ten monoclonal antibodies(mAbs)against gliadin and after optimization were obtained,a pair of mAbs suitable for its detection(capture antibody:mAb 7;detection antibody:HRP-labeled mAb 6)were identified.Based on two of these antibodies,a sandwich ELISA with limit of detection(LOD)of 60 ng/mL in negative milk,and more importantly,negligible cross-reactivity to other allergens was developed.The average recoveries for gliadin in negative milk were 99.16%-100.07%using the sandwich ELISA.We also developed LFIA strips for the rapid detection of gliadin with visual limit of detection(vLOD)of 25 ng/mL and calculated LOD value of 6.56 ng/mL in negative milk.The test results of positive samples obtained from the LFIA strips were highly consistent with those of the sandwich ELISA.Thus,the developed LFIA strip is an effective and reliable tool for the rapid and on-site detection of wheat allergen in milk.
基金supported by the National Natural Science Foundation of China(Grant No.81571835,61503246,81672247 and 81671737)National Key Basic Research Program(973 Project)(No.2015CB931802)+1 种基金the 863 High-Tech Project of China(No.2014AA020700)Shanghai Science and Technology Fund(No.13NM1401500 and 15DZ2252000).
文摘Point-of-care testing(POCT)is essential for providing a rapid diagnostic result in a prompt on-site diagnosis and treatment.A quick analysis time and a high sensitivity,with a sample-to-answer format,are the most important features for current POCT diagnostic systems.This review covers recent advances in POCT technologies with an emphasis on demonstrated and commercially available POCT diagnostic systems with laboratory quality using lateral flow immunoassay(LFIA).The system includes the integration of nanoparticles(NPs)in lateral flow test strips(LFTSs)and the mechanism through which particles improve the analytical performance of the fabricated strips.Several examples of NP-based LFTSs were selected to illustrate novel concepts or devices with promising applications as screening tools and superior alternatives to existing conventional strategies in clinical analysis,food safety,and environmental monitoring.In each analyte category,detection methods,configuration of LFIA modules,and advantages of POCT systems are reviewed and discussed along with future prospects.This review also discusses novel signal-enhancement strategies,optimal reader systems,and multiplex design prototypes,which have been employed for highly sensitive multiplex assay of LFTSs.
