目的观察一氧化氮合酶抑制剂[L-NG-硝基精氨酸甲酯(L-NG-n itroargin ine m ethylester,L-NAME)]和钙蛋白酶抑制剂[leupeptin]对庆大霉素(gentam ic in,GM)所致耳蜗毛细胞损伤的协同防护作用。方法将20只出生后3~5天的W istar大鼠(40耳...目的观察一氧化氮合酶抑制剂[L-NG-硝基精氨酸甲酯(L-NG-n itroargin ine m ethylester,L-NAME)]和钙蛋白酶抑制剂[leupeptin]对庆大霉素(gentam ic in,GM)所致耳蜗毛细胞损伤的协同防护作用。方法将20只出生后3~5天的W istar大鼠(40耳)平均分成5组,1组为正常对照,其余4组在施加GM的同时单独或联合应用不同的保护因素(L-NAME和leupeptin)。通过耳蜗器官离体培养,对基底膜铺片行TR ITC-Phalloid in荧光染色,观察各组耳蜗毛细胞的生长情况并比较存活率。结果GM组毛细胞存活极少,L-NAME和leupeptin单独应用时毛细胞存活率明显高于GM组(P<0.01),二者联合应用时耳蜗毛细胞存活率显著高于L-NAME和leupeptin单独用药组(P<0.01)。结论L-NAME和leupeptin对庆大霉素耳毒性所致毛细胞损伤具有较好的协同防护作用。展开更多
BACKGROUND： Porphyromonas gingivalis is a periodontal pathogen, which is considered to be a keystone pathogen for periodontitis. A diverse conglomerate of P. gingivalis virulence factors including lipopolysaccharide,...BACKGROUND： Porphyromonas gingivalis is a periodontal pathogen, which is considered to be a keystone pathogen for periodontitis. A diverse conglomerate of P. gingivalis virulence factors including lipopolysaccharide, fimbriae, capsular polysaccharide, haemagglutinin and cysteine proteases （Arg-gingipains and Lys-gingipain） are considered to be involved in the pathogenesis ofperiodontitis. Leupeptin is a cysteine protease inhibitor which is specific for Arg gingipains. The present review focuses on action of leupeptin on Arg gingipains. METHOD： A search was carried out systematically from the start till September, 2016. The search was made in Medline database via PubMed. The keywords enlisted were ＂leupeptin＂; ＂gingipains＂; ＂periodontitis＂ using Boolean operator ＂and.＂ RESULTS： The result was selection of 58 articles which linked leupeptin to periodontitis and gingipains; pathogenesis of periodontitis, pathogenicity of gingipains and role of leupeptin. CONCLUSION： It was concluded that leupeptin inhibits and attenuates a number of destructive activities of Arg gingipains including inhibition ofplatelet aggregation; inhibit degradation of LL-37, which is an antimicrobial peptide; blocking inhibition ofmonocyte chemoattractant protein; restoring level ofinterleukin-2; inhibiting degradation of collagen type I and IV to name a few.展开更多
文摘目的观察一氧化氮合酶抑制剂[L-NG-硝基精氨酸甲酯(L-NG-n itroargin ine m ethylester,L-NAME)]和钙蛋白酶抑制剂[leupeptin]对庆大霉素(gentam ic in,GM)所致耳蜗毛细胞损伤的协同防护作用。方法将20只出生后3~5天的W istar大鼠(40耳)平均分成5组,1组为正常对照,其余4组在施加GM的同时单独或联合应用不同的保护因素(L-NAME和leupeptin)。通过耳蜗器官离体培养,对基底膜铺片行TR ITC-Phalloid in荧光染色,观察各组耳蜗毛细胞的生长情况并比较存活率。结果GM组毛细胞存活极少,L-NAME和leupeptin单独应用时毛细胞存活率明显高于GM组(P<0.01),二者联合应用时耳蜗毛细胞存活率显著高于L-NAME和leupeptin单独用药组(P<0.01)。结论L-NAME和leupeptin对庆大霉素耳毒性所致毛细胞损伤具有较好的协同防护作用。
文摘BACKGROUND： Porphyromonas gingivalis is a periodontal pathogen, which is considered to be a keystone pathogen for periodontitis. A diverse conglomerate of P. gingivalis virulence factors including lipopolysaccharide, fimbriae, capsular polysaccharide, haemagglutinin and cysteine proteases （Arg-gingipains and Lys-gingipain） are considered to be involved in the pathogenesis ofperiodontitis. Leupeptin is a cysteine protease inhibitor which is specific for Arg gingipains. The present review focuses on action of leupeptin on Arg gingipains. METHOD： A search was carried out systematically from the start till September, 2016. The search was made in Medline database via PubMed. The keywords enlisted were ＂leupeptin＂; ＂gingipains＂; ＂periodontitis＂ using Boolean operator ＂and.＂ RESULTS： The result was selection of 58 articles which linked leupeptin to periodontitis and gingipains; pathogenesis of periodontitis, pathogenicity of gingipains and role of leupeptin. CONCLUSION： It was concluded that leupeptin inhibits and attenuates a number of destructive activities of Arg gingipains including inhibition ofplatelet aggregation; inhibit degradation of LL-37, which is an antimicrobial peptide; blocking inhibition ofmonocyte chemoattractant protein; restoring level ofinterleukin-2; inhibiting degradation of collagen type I and IV to name a few.
