The mimetic peroxidase iron-tetrakis(3-N-methylpyridyl)- porphyrin (Fe-T(3MPy)P)can be used as a catalyst in the spectrophoto- metric determination of H_2O_2 and glucose.The method is based on the formation of crystal...The mimetic peroxidase iron-tetrakis(3-N-methylpyridyl)- porphyrin (Fe-T(3MPy)P)can be used as a catalyst in the spectrophoto- metric determination of H_2O_2 and glucose.The method is based on the formation of crystal violet from leuco crystal violet.The glucose in human serum is determined by this reaction combined with catalytic reaction of glucose oxidase.展开更多
A systematic study of the synthesis of C.I.Acid Blue 9 leuco compound in water is reported.The kinetic analysis of experimental data for the condensation reaction between 2-formylbenzenesulfonic acid sodium and N-ethy...A systematic study of the synthesis of C.I.Acid Blue 9 leuco compound in water is reported.The kinetic analysis of experimental data for the condensation reaction between 2-formylbenzenesulfonic acid sodium and N-ethyl-N-(3'-sulfonic acid benzyl) aniline obtained at four different temperatures ranging between 85 and 100°C is discussed.It is shown that the reaction followed second-order rate kinetics.The overall rate constant(k) increased with the increase of temperature.On the basis of the value of k,activation energy(E_a) of the reaction was evaluated.Importantly,it is found that reactant concentration has great effect on the formation of C.I.Acid Blue 9 leuco compound,implying that it is not enough to improve the conversion of N-ethyl-N-(3'-sulfonic acid benzyl) aniline by only prolonging reaction time in the late period of the reaction.展开更多
文摘The mimetic peroxidase iron-tetrakis(3-N-methylpyridyl)- porphyrin (Fe-T(3MPy)P)can be used as a catalyst in the spectrophoto- metric determination of H_2O_2 and glucose.The method is based on the formation of crystal violet from leuco crystal violet.The glucose in human serum is determined by this reaction combined with catalytic reaction of glucose oxidase.
基金Supported by the National Natural Science Foundation of China(U1608223,21576044,21421005,21536002)the Dalian University of Technology Innovation Team(DUT2016TB12)
文摘A systematic study of the synthesis of C.I.Acid Blue 9 leuco compound in water is reported.The kinetic analysis of experimental data for the condensation reaction between 2-formylbenzenesulfonic acid sodium and N-ethyl-N-(3'-sulfonic acid benzyl) aniline obtained at four different temperatures ranging between 85 and 100°C is discussed.It is shown that the reaction followed second-order rate kinetics.The overall rate constant(k) increased with the increase of temperature.On the basis of the value of k,activation energy(E_a) of the reaction was evaluated.Importantly,it is found that reactant concentration has great effect on the formation of C.I.Acid Blue 9 leuco compound,implying that it is not enough to improve the conversion of N-ethyl-N-(3'-sulfonic acid benzyl) aniline by only prolonging reaction time in the late period of the reaction.
