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病毒诱导烟草的基因沉默 被引量:6
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作者 傅达奇 朱本忠 +3 位作者 赵晓丹 朱宏亮 谢远红 罗云波 《西北植物学报》 CAS CSCD 北大核心 2005年第8期1517-1520,共4页
病毒诱导基因沉默是利用RNA介导病毒防卫机制的一项技术.构建含有目的基因片段的人工改造病毒载体,通过农杆菌侵染导致植物内源目的基因沉默.为建立病毒诱导基因沉默体系,选用烟草脆裂病毒(TRV)和烟草为实验材料.构建了八氢番茄红素去... 病毒诱导基因沉默是利用RNA介导病毒防卫机制的一项技术.构建含有目的基因片段的人工改造病毒载体,通过农杆菌侵染导致植物内源目的基因沉默.为建立病毒诱导基因沉默体系,选用烟草脆裂病毒(TRV)和烟草为实验材料.构建了八氢番茄红素去饱和酶基因(PDS)的基因沉默病毒载体,病毒载体侵染结果显示目的基因PDS沉默导致烟草幼苗出现光漂白现象.采用RT-PCR的方法检测目的基因PDS的沉默效果,结果显示PDS基因mRNA被显著降解.该体系的建立有利于将来对植物基因进行高通量功能分析. 展开更多
关键词 烟草脆裂病毒 基因沉默 烟草 lepdS
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A geometric factor calculation method based on the isotropic f lux assumption
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作者 赵小芸 王焕玉 +12 位作者 吴峰 孟祥承 马宇倩 卢红 王辉 王平 李新乔 徐岩冰 石峰 蒋文奇 安正华 于晓霞 刘汉一 《Chinese Physics C》 SCIE CAS CSCD 2013年第12期78-83,共6页
One of the instruments onboard the China Seismic Electromagnetic Satellite (CSES) is the Low Energy Particle Detector (LEPD). The primary objective of LEPD is to provide measurements of the fluxes, energy spectra ... One of the instruments onboard the China Seismic Electromagnetic Satellite (CSES) is the Low Energy Particle Detector (LEPD). The primary objective of LEPD is to provide measurements of the fluxes, energy spectra and pitch angles of 100 keV to 10 MeV electrons and protons from 2 to 50 MeV in the Earth's magnetosphere. The geometric factor is one of the principle parameters of a detector, which converts the physical quantity-count rate to the particle quantity-flux. In this paper, we calculated the geometric factor of LEPD via computer modeling of an isotropic radiation environment. It was first demonstrated that the radiation intensity related should obey a cosine-law, then a general sampling method of generating this distribution via GPS of GEANT4 was explained. Furthermore, combined with flux normalization, a comparison of the geometric factor calculation of a set of 2-layer detectors with different shapes (cylinder, truncated cone and rectangle) was performed. Results show a generally good agreement between simulation and analytical calculations for the cylinder and truncated cone detectors, and the result of the rectangular one, for which there is no accurate analytical formula, is consistent with the previous simulated results by others. As a practical instance of the 2-layer rectangle detector, the geometric factor of LEPD is 10.336±0.036 m cm2·sr for 10 MeV proton and 8.211±0.032 m cm2·sr for 8 MeV electron. 展开更多
关键词 lepd geometric factor ISOTROPY GEANT4 cosine distribution flux normalization
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