Objectives:Recently,we and others have demonstrated the involvement of Zinc Finger Antisense 1(ZFAS1)in cancer development.However,the intricate interplay of ZFAS1 with miRNAs and mRNAs remains to be fully understood....Objectives:Recently,we and others have demonstrated the involvement of Zinc Finger Antisense 1(ZFAS1)in cancer development.However,the intricate interplay of ZFAS1 with miRNAs and mRNAs remains to be fully understood.Materials and methods:We followed PRISMA guidelines to retrieve and assess the available literature on the topic“ZFAS1/miRNA/mRNA axis”and“Cancer”from databases such as PubMed,Google Scholar,and ScienceDirect.We also used bioinformatic webtools for analyzing the potential miRNA targets of ZFAS1 and its role in survival of cancer patients along with their role in various biological functions and pathways.Results:Our literature search and bioinformatic analysis reveals that ZFAS1 serves as a sponge for numerous miRNAs.Among the various targeted miRNAs,miR-150-5p stands out as significantly correlated with ZFAS1 across multiple databases(p-value=3.27e-16,R-value=-0.346).Additionally,our Kaplan-Meier survival analysis indicates a noteworthy association between ZFAS1 expression levels and overall poor prognosis and survival rates in ovarian,sarcoma,and pancreatic cancers.We also underscore the involvement of various signaling pathways,including Signal Transducer and Activator of Transcription 3(STAT3),Spindle and Kinetochore-associated Protein 1(SKA1),Lysophosphatidic acid receptor 1(LPAR1),and Wntβ-catenin,in cancer development through the ZFAS1/miRNAs/mRNAs axis.Furthermore,we identify ZFAS1’s pivotal roles in diverse molecular processes,such as RNA binding and ribonucleoprotein formation.Conclusion:In conclusion,this review comprehensively summarizes the latest advancements in understanding the regulatory relationships among ZFAS1,miRNAs,and mRNAs,emphasizing their collective role in cancer development to propose innovative avenues for cancer treatment.We believe that the intricate relationship among the ZFAS1-miRNA-mRNA axis may yield potential therapeutic targets for effective cancer management.展开更多
[Objectives]To further explore the mechanism of quercetin regulating the activity of Sune-1 cells.[Methods]High-throughput mRNA-miRNA transcriptome sequencing technology was used to screen miRNA in Sune-1 cells treate...[Objectives]To further explore the mechanism of quercetin regulating the activity of Sune-1 cells.[Methods]High-throughput mRNA-miRNA transcriptome sequencing technology was used to screen miRNA in Sune-1 cells treated with quercetin.[Results]Statistical analysis showed that 1264 miRNAs were differentially expressed in Sune-1 cells treated with quercetin,of which 716 were significantly up-regulated and 548 were significantly down-regulated;191 miRNAs were differentially expressed in Sune-1 cells treated with quercetin,of which 129 were significantly up-regulated and 62 were significantly down-regulated.By comparing the expression differences of these mRNAs and miRNAs in different samples,six different expression patterns were clustered.The expression of the above miRNAs was verified by real-time quantitative PCR(qPCR),and the results were highly consistent with the transcriptome sequencing data.In addition,Gene Ontology annotation and functional enrichment analysis of miRNA target genes showed that CTGF,VHL and H19,which are related to the regulation of cell proliferation signal transduction,were predicted to be new targets of differential miRNAs such as miR494-3p and miR675-3p and may play an important regulatory role in the process of Quercetin inhibiting the proliferation of Sune-1 cells.[Conclusions]This study provides a basis for the rational use of anti-tumor functional components of traditional Chinese medicine,and also provides a theoretical basis for the targeted therapy of nasopharyngeal carcinoma.展开更多
Background:Ovarian cancer(OC)is a representative malignancy of the female reproductive system,with a poor prognosis.Long non-coding RNAs(lncRNAs)crucially affect tumor development.This study aimed to identify lncRNAs ...Background:Ovarian cancer(OC)is a representative malignancy of the female reproductive system,with a poor prognosis.Long non-coding RNAs(lncRNAs)crucially affect tumor development.This study aimed to identify lncRNAs that potentially participated in OC.Methods:LncRNA expression in cells and tissues was quantified using reverse transcription-quantitative PCR,while fluorescence in situ hybridization determined their cellular localization.Various in vitro assays,together with a mouse xenograft model,were employed to elucidate the function of CYMP antisense RNA 1(CYMP-AS1)in OC.The molecular mechanisms underlying CYMP-AS1 regulation were investigated through RNA pull-down and immunoprecipitation assays,immunofluorescence staining,western blotting,and mRNA stability assays.Results:This study identified a previously unreported lncRNA,CYMP-AS1,which exhibits increased expression in the cytoplasm of OC tissues and cells.Knockout of CYMP-AS1 reduced the OC cell proliferation,migration,invasion,and epithelial-mesenchymal transition(EMT).CYMP-AS1 directly interacts with heterogeneous nuclear ribonucleoprotein M(hnRNPM),inducing its intracellular translocation and reducing the stability of Axis inhibition protein 2(AXIN2)mRNA.This process ultimately elevated the expression of Wnt/β-catenin signaling pathway-related proteins.Conclusion:This study confirms CYMP-AS1 as a novel biomarker in OC progression and suggests that the CYMP-AS1/hnRNPM/AXIN2 axis may offer an innovative strategy for OC treatment.展开更多
文摘目的探讨人牙周膜细胞在流体剪切力作用下淋巴样增强因子-1(lymphoid enhancer factor-1,LEF-1)m RNA的表达。方法对人牙周膜细胞进行原代培养;通过流体剪切力加力系统对人牙周膜细胞施加1.2 Pa的流体剪切力,作用0、0.5、2、4、8 h后,用RT-PCR方法检测细胞受力前后LEF-1 m RNA的表达变化。结果人牙周膜细胞在1.2 Pa的流体剪切力作用8 h后,LEF-1 m RNA的表达升高。结论流体剪切力刺激活化了人牙周膜细胞中LEF-1的转录,LEF-1 m RNA的表达升高,可能是在剪切应力作用下Wnt信号通路的应答反应。
文摘Objectives:Recently,we and others have demonstrated the involvement of Zinc Finger Antisense 1(ZFAS1)in cancer development.However,the intricate interplay of ZFAS1 with miRNAs and mRNAs remains to be fully understood.Materials and methods:We followed PRISMA guidelines to retrieve and assess the available literature on the topic“ZFAS1/miRNA/mRNA axis”and“Cancer”from databases such as PubMed,Google Scholar,and ScienceDirect.We also used bioinformatic webtools for analyzing the potential miRNA targets of ZFAS1 and its role in survival of cancer patients along with their role in various biological functions and pathways.Results:Our literature search and bioinformatic analysis reveals that ZFAS1 serves as a sponge for numerous miRNAs.Among the various targeted miRNAs,miR-150-5p stands out as significantly correlated with ZFAS1 across multiple databases(p-value=3.27e-16,R-value=-0.346).Additionally,our Kaplan-Meier survival analysis indicates a noteworthy association between ZFAS1 expression levels and overall poor prognosis and survival rates in ovarian,sarcoma,and pancreatic cancers.We also underscore the involvement of various signaling pathways,including Signal Transducer and Activator of Transcription 3(STAT3),Spindle and Kinetochore-associated Protein 1(SKA1),Lysophosphatidic acid receptor 1(LPAR1),and Wntβ-catenin,in cancer development through the ZFAS1/miRNAs/mRNAs axis.Furthermore,we identify ZFAS1’s pivotal roles in diverse molecular processes,such as RNA binding and ribonucleoprotein formation.Conclusion:In conclusion,this review comprehensively summarizes the latest advancements in understanding the regulatory relationships among ZFAS1,miRNAs,and mRNAs,emphasizing their collective role in cancer development to propose innovative avenues for cancer treatment.We believe that the intricate relationship among the ZFAS1-miRNA-mRNA axis may yield potential therapeutic targets for effective cancer management.
基金Supported by Educational Research Project for Young and Middle-aged Teachers in Fujian Province(Science and Technology Category,JAT210477)。
文摘[Objectives]To further explore the mechanism of quercetin regulating the activity of Sune-1 cells.[Methods]High-throughput mRNA-miRNA transcriptome sequencing technology was used to screen miRNA in Sune-1 cells treated with quercetin.[Results]Statistical analysis showed that 1264 miRNAs were differentially expressed in Sune-1 cells treated with quercetin,of which 716 were significantly up-regulated and 548 were significantly down-regulated;191 miRNAs were differentially expressed in Sune-1 cells treated with quercetin,of which 129 were significantly up-regulated and 62 were significantly down-regulated.By comparing the expression differences of these mRNAs and miRNAs in different samples,six different expression patterns were clustered.The expression of the above miRNAs was verified by real-time quantitative PCR(qPCR),and the results were highly consistent with the transcriptome sequencing data.In addition,Gene Ontology annotation and functional enrichment analysis of miRNA target genes showed that CTGF,VHL and H19,which are related to the regulation of cell proliferation signal transduction,were predicted to be new targets of differential miRNAs such as miR494-3p and miR675-3p and may play an important regulatory role in the process of Quercetin inhibiting the proliferation of Sune-1 cells.[Conclusions]This study provides a basis for the rational use of anti-tumor functional components of traditional Chinese medicine,and also provides a theoretical basis for the targeted therapy of nasopharyngeal carcinoma.
基金funded by the National Natural Science Foundation of China(No.8207286).
文摘Background:Ovarian cancer(OC)is a representative malignancy of the female reproductive system,with a poor prognosis.Long non-coding RNAs(lncRNAs)crucially affect tumor development.This study aimed to identify lncRNAs that potentially participated in OC.Methods:LncRNA expression in cells and tissues was quantified using reverse transcription-quantitative PCR,while fluorescence in situ hybridization determined their cellular localization.Various in vitro assays,together with a mouse xenograft model,were employed to elucidate the function of CYMP antisense RNA 1(CYMP-AS1)in OC.The molecular mechanisms underlying CYMP-AS1 regulation were investigated through RNA pull-down and immunoprecipitation assays,immunofluorescence staining,western blotting,and mRNA stability assays.Results:This study identified a previously unreported lncRNA,CYMP-AS1,which exhibits increased expression in the cytoplasm of OC tissues and cells.Knockout of CYMP-AS1 reduced the OC cell proliferation,migration,invasion,and epithelial-mesenchymal transition(EMT).CYMP-AS1 directly interacts with heterogeneous nuclear ribonucleoprotein M(hnRNPM),inducing its intracellular translocation and reducing the stability of Axis inhibition protein 2(AXIN2)mRNA.This process ultimately elevated the expression of Wnt/β-catenin signaling pathway-related proteins.Conclusion:This study confirms CYMP-AS1 as a novel biomarker in OC progression and suggests that the CYMP-AS1/hnRNPM/AXIN2 axis may offer an innovative strategy for OC treatment.
