Medicinal components of Hypericum perforatum L.plants varies widely due to fluctuations in growth environment and biotic and abiotic contamination during cultivation management.The quality of extracts or preparations ...Medicinal components of Hypericum perforatum L.plants varies widely due to fluctuations in growth environment and biotic and abiotic contamination during cultivation management.The quality of extracts or preparations is difficult to control because of the unstable raw materials.The aim of this study is to enhance the yield and medicinal component contents of H.perforatum by optimizing lighting factors under controlled environment.H.perforatum plants were hydroponically cultivated for 30 d under 3 levels of photosynthetic photon flux density(PPFD)with 200,300,and 400μmol/(m^(2)·s)using white LEDs(R:B ratio is the ratio of red light to blue light,R:B ratio of 0.9 and 1.8)and white plus red LED(R:B ratio of 2.7).The results showed that PPFD and LED spectrum had significant effects on the growth and accumulation of medicinal components of H.perforatum.Biomass accumulation of stem,leaf,and root increased linearly with the increase of PPFD under each LED spectrum.Fresh weights and dry weights of stem,leaf,and root were significantly higher under a PPFD of 400μmol/(m^(2)·s)with R:B ratio of 0.9 than those of 200μmol/(m^(2)·s),respectively.The relative growth rate and net photosynthetic rate showed linear relationships with PPFD under the same LED spectrum.Total hypericin content,total hyperforin content,and energy yield of hypericin increased with increasing PPFD.Total hypericin content and energy yield of hypericin of P400-L0.9 were 78%and 89%more than those of P400-L2.7,respectively.Total hyperforin content and energy yield of hyperforin of P400-L0.9 and P400-L2.7 were no significant differences.Based on energy efficiency,an R:B ratio of 0.9 of white LEDs with a PPFD of 400μmol/(m^(2)·s)was beneficial to improve medicinal component contents of hydroponic H.perforatum in plant factory with LED lighting.展开更多
Light spectrum plays an important role in regulating the growth and development of in vitro cultured potato(Solanum tuberosum L.) plantlets. The status of potato plantlets at the end of in vitro stage influences the m...Light spectrum plays an important role in regulating the growth and development of in vitro cultured potato(Solanum tuberosum L.) plantlets. The status of potato plantlets at the end of in vitro stage influences the minituber production after transplanting. With 100 μmol m^-2s^-1 total photosynthetic photon flux density(PPFD), a light spectrum study of 100% red light emitting diodes(LEDs) light spectrum(RR), 100% blue LEDs light spectrum(BB), 65% red+35% blue LEDs light spectrum(RB), and 45% red+35% blue+20% green LEDs light spectrum(RBG) providing illumination at the in vitro cultured stage of potato plantlets for 4 weeks using fluorescent lamp as control(CK) was performed to investigate the effects of LEDs light spectrum on the growth, leaf anatomy, and chloroplast ultrastructure of potato plantlets in vitro as well as the minituber yield after 2 months transplanting in the greenhouse. Compared to CK, RB and RBG promoted the growth of potato plantlets in vitro with increased stem diameter, plantlet fresh weight, plantlet dry weight, and health index. Furthermore, BB induced the greatest stem diameter as well as the highest health index in potato plantlets in vitro. Root activity, soluble protein, and free amino acid were also significantly enhanced by BB, whereas carbohydrates were improved by RR. In addition, thickness of leaf, palisade parenchyma and spongy parenchyma was significantly increased by BB and RBG. Chloroplasts under BB and RBG showed well-developed grana thylakoid and stroma thylakoid. Unexpectedly, distinct upper epidermis with greatest thickness was induced and palisade parenchyma and spongy parenchyma were arranged neatly in RR. After transplanting in the greenhouse for 2 months, potato plantlets in vitro from BB, RB, and RBG produced high percentage of large size tuber. BB improved fresh and dry weights of the biggest tuber but decreased tuber number per plantlet. In addition, RBG increased tuber number as well as tuber fresh and dry weight slightly. Our results suggested monochromatic blue LEDs as well as combined red, blue or/and green LEDs light spectrum were superior to fluorescent lamp spectrum in micro-propagation of potato plantlets. Therefore, the application of RBG was suitable;BB and RB could be used as alternatives.展开更多
基金supported by China Agriculture Research System of MOF and MARA(CARS-21).
文摘Medicinal components of Hypericum perforatum L.plants varies widely due to fluctuations in growth environment and biotic and abiotic contamination during cultivation management.The quality of extracts or preparations is difficult to control because of the unstable raw materials.The aim of this study is to enhance the yield and medicinal component contents of H.perforatum by optimizing lighting factors under controlled environment.H.perforatum plants were hydroponically cultivated for 30 d under 3 levels of photosynthetic photon flux density(PPFD)with 200,300,and 400μmol/(m^(2)·s)using white LEDs(R:B ratio is the ratio of red light to blue light,R:B ratio of 0.9 and 1.8)and white plus red LED(R:B ratio of 2.7).The results showed that PPFD and LED spectrum had significant effects on the growth and accumulation of medicinal components of H.perforatum.Biomass accumulation of stem,leaf,and root increased linearly with the increase of PPFD under each LED spectrum.Fresh weights and dry weights of stem,leaf,and root were significantly higher under a PPFD of 400μmol/(m^(2)·s)with R:B ratio of 0.9 than those of 200μmol/(m^(2)·s),respectively.The relative growth rate and net photosynthetic rate showed linear relationships with PPFD under the same LED spectrum.Total hypericin content,total hyperforin content,and energy yield of hypericin increased with increasing PPFD.Total hypericin content and energy yield of hypericin of P400-L0.9 were 78%and 89%more than those of P400-L2.7,respectively.Total hyperforin content and energy yield of hyperforin of P400-L0.9 and P400-L2.7 were no significant differences.Based on energy efficiency,an R:B ratio of 0.9 of white LEDs with a PPFD of 400μmol/(m^(2)·s)was beneficial to improve medicinal component contents of hydroponic H.perforatum in plant factory with LED lighting.
基金supported by the National High-Tech R&D Program of China (2013 AA 103005)
文摘Light spectrum plays an important role in regulating the growth and development of in vitro cultured potato(Solanum tuberosum L.) plantlets. The status of potato plantlets at the end of in vitro stage influences the minituber production after transplanting. With 100 μmol m^-2s^-1 total photosynthetic photon flux density(PPFD), a light spectrum study of 100% red light emitting diodes(LEDs) light spectrum(RR), 100% blue LEDs light spectrum(BB), 65% red+35% blue LEDs light spectrum(RB), and 45% red+35% blue+20% green LEDs light spectrum(RBG) providing illumination at the in vitro cultured stage of potato plantlets for 4 weeks using fluorescent lamp as control(CK) was performed to investigate the effects of LEDs light spectrum on the growth, leaf anatomy, and chloroplast ultrastructure of potato plantlets in vitro as well as the minituber yield after 2 months transplanting in the greenhouse. Compared to CK, RB and RBG promoted the growth of potato plantlets in vitro with increased stem diameter, plantlet fresh weight, plantlet dry weight, and health index. Furthermore, BB induced the greatest stem diameter as well as the highest health index in potato plantlets in vitro. Root activity, soluble protein, and free amino acid were also significantly enhanced by BB, whereas carbohydrates were improved by RR. In addition, thickness of leaf, palisade parenchyma and spongy parenchyma was significantly increased by BB and RBG. Chloroplasts under BB and RBG showed well-developed grana thylakoid and stroma thylakoid. Unexpectedly, distinct upper epidermis with greatest thickness was induced and palisade parenchyma and spongy parenchyma were arranged neatly in RR. After transplanting in the greenhouse for 2 months, potato plantlets in vitro from BB, RB, and RBG produced high percentage of large size tuber. BB improved fresh and dry weights of the biggest tuber but decreased tuber number per plantlet. In addition, RBG increased tuber number as well as tuber fresh and dry weight slightly. Our results suggested monochromatic blue LEDs as well as combined red, blue or/and green LEDs light spectrum were superior to fluorescent lamp spectrum in micro-propagation of potato plantlets. Therefore, the application of RBG was suitable;BB and RB could be used as alternatives.
