Leaf spot disease seriously affects the yield and quality of waxberry, but there is no registration agent for prevention and control of the disease. The field trials showed that the control efficacy of 33.5% oxine-cop...Leaf spot disease seriously affects the yield and quality of waxberry, but there is no registration agent for prevention and control of the disease. The field trials showed that the control efficacy of 33.5% oxine-copper SC against leaf spot disease on waxberry reached above 74% when used at the dosage of 167.5-223.3 mg/kg. 33.5% Oxine-copper SC could be used to control leaf spot disease on waxberry.展开更多
[Objectives]The paper was to identify the causal agent of leaf spot of banana in Hainnan,China.[Methods]Fungus isolates were isolated from the diseased tissues,and morphological characteristics of colony and spore wer...[Objectives]The paper was to identify the causal agent of leaf spot of banana in Hainnan,China.[Methods]Fungus isolates were isolated from the diseased tissues,and morphological characteristics of colony and spore were observed.Furthermore,phylogenetic analyses and pathogenicity test were conducted to confirm the pathogen.[Results]The fungus isolates from the diseased tissues was identified as Curvularia geniculata.[Conclusions]C.geniculata is a new pathogen causing leaf spot of banana in Hainan,China.This finding will help to broaden the distribution and host range of C.geniculata,indicating that it poses potential damage to banana in China.展开更多
Robbsia andropogonis is one of the most destructive leaf spot disease pathogens of numerous host plants and causes heavy economic damage.In the present study,the complete genome of R.andropogonis strain BLB1,causing t...Robbsia andropogonis is one of the most destructive leaf spot disease pathogens of numerous host plants and causes heavy economic damage.In the present study,the complete genome of R.andropogonis strain BLB1,causing the leaf spot disease of areca palm,was generated using a hybrid method combining ONT PromethION long reads and BGISEQ-500 short reads.The resulting genome consists of seven replicons totaling 6,828,120 bp,and 5,808 genes were annotated,including 788 virulence-related genes.Function analysis showed that genes involved in metabolism were the most abundant group.Impressively,the bacteria were well-equipped with four,two,and four sets of type three,four,and six secretion systems,respectively,highlighting the virulence features of R.andropogonis BLB1.As the first complete genome sequence of the species of genus Robbsia,the BLB1 genome provides a solid foundation for investigation of mechanisms underlying the pathogen virulence and disease control,and will promote further discovery and characterization of the genus Robbsia.展开更多
Aiming at the problem of low detection accuracy due to the different scale sizes of apple leaf disease spots and their similarity to the background,this paper proposes a multi-scale lightweight network(MSL-Net).Firstl...Aiming at the problem of low detection accuracy due to the different scale sizes of apple leaf disease spots and their similarity to the background,this paper proposes a multi-scale lightweight network(MSL-Net).Firstly,a multiplexed aggregated feature extraction network is proposed using residual bottleneck block(RES-Bottleneck)and middle partial-convolution(MP-Conv)to capture multi-scale spatial features and enhance focus on disease features for better differentiation between disease targets and background information.Secondly,a lightweight feature fusion network is designed using scale-fuse concatenation(SF-Cat)and triple-scale sequence feature fusion(TSSF)module to merge multi-scale feature maps comprehensively.Depthwise convolution(DWConv)and GhostNet lighten the network,while the cross stage partial bottleneck with 3 convolutions ghost-normalization attention module(C3-GN)reduces missed detections by suppressing irrelevant background information.Finally,soft non-maximum suppression(Soft-NMS)is used in the post-processing stage to improve the problem of misdetection of dense disease sites.The results show that the MSL-Net improves mean average precision at intersection over union of 0.5(mAP@0.5)by 2.0%over the baseline you only look once version 5s(YOLOv5s)and reduces parameters by 44%,reducing computation by 27%,outperforming other state-of-the-art(SOTA)models overall.This method also shows excellent performance compared to the latest research.展开更多
[Objectives]This study was conducted to investigate the control of Chinese herbal medicine regulators on leaf spot nematode disease,the main pest of Chloranthus spicatus(Thunb.)Makino.[Methods]C.spicatus plants infect...[Objectives]This study was conducted to investigate the control of Chinese herbal medicine regulators on leaf spot nematode disease,the main pest of Chloranthus spicatus(Thunb.)Makino.[Methods]C.spicatus plants infected with nematodes were irrigated with a Chinese herbal medicine regulator at two different concentrations,and the control effect on leaf spot nematode disease of the plant was studied by measuring the number of nematodes,the contents of chlorophyll,malondialdehyde,soluble sugar and free proline in the leaves of the plants in the soil.[Results]Compared with the control,the two concentrations of Chinese herbal medicine regulator significantly reduced the number of nematodes in the soil,increased the contents of chlorophyll(mainly chlorophyll a),soluble sugar and free proline in the leaves of C.spicatus,and decreased the content of malondialdehyde.It indicated that the Chinese herbal medicine regulator could effectively control the spread of nematodes and had certain effect on the recovery of C.spicatus plants.[Conclusions]The use of Chinese herbal medicine regulators is helpful to curb the occurrence of leaf spot nematode disease in C.spicatus by biological prevention and control means,and provides theoretical guidance for the development of the green industry of C.spicatus.展开更多
[Objectives]The paper was to study molecular characterization,morphology and pathogenicity of Curvularia pseudobrachyspora,a new causal agent of leaf spot on banana.[Methods]Banana(Musa acuminate)leaves with streaks o...[Objectives]The paper was to study molecular characterization,morphology and pathogenicity of Curvularia pseudobrachyspora,a new causal agent of leaf spot on banana.[Methods]Banana(Musa acuminate)leaves with streaks or long ellipse-shaped lesions were sampled in an orchard of Danzhou City,Hainan Province,China in 2021.Fungus isolates were isolated from the diseased tissues and further identified as C.pseudobrachyspora based on morphological characteristics of colony,conidiophore and spore,phylogenetic analyses of the ITS region,GAPDH and TEF-1αgenes.[Results]In the pathogenicity test,the fungus re-isolated from inoculated leaves with necrotic lesions was identified morphologically and molecularly,fulfilling Koch's postulates.[Conclusions]C.pseudobrachyspora is a new pathogen causing leaf spot of banana in China and the world.展开更多
[Objectives]The study was to identify the causal agent of leaf spots of Camellia drupifera in Hainan Province,China.[Methods]Phylogenetic analyses,spore morphology and pathogenicity tests were adopted to identify the ...[Objectives]The study was to identify the causal agent of leaf spots of Camellia drupifera in Hainan Province,China.[Methods]Phylogenetic analyses,spore morphology and pathogenicity tests were adopted to identify the pathogen.[Results]The fungus was identified as Neofusicoccum parvum.The colonies were initially pale to white on PDA,with diffuse yellow pigment around the colonies in the agar after 5 d.After 10 d,the aerial mycelium became gray,and the substrate mycelium became black.Thirty days later,a large number of conidia were generated on OMA.Conidia were hyaline,nonseptate,and fusiform.The mean size of 100 conidia was(13.6-25.4)×(6.2-10.3)μm;the mean length/width ratio was(2.5±0.3)μm.[Conclusions]N.parvum was the causal agent of leaf spots of C.drupifera observed in Hainan Province,China.展开更多
Soybean frogeye leaf spot(FLS) disease is a global disease affecting soybean yield, especially in the soybean growing area of Heilongjiang Province. In order to realize genomic selection breeding for FLS resistance of...Soybean frogeye leaf spot(FLS) disease is a global disease affecting soybean yield, especially in the soybean growing area of Heilongjiang Province. In order to realize genomic selection breeding for FLS resistance of soybean, least absolute shrinkage and selection operator(LASSO) regression and stepwise regression were combined, and a genomic selection model was established for 40 002 SNP markers covering soybean genome and relative lesion area of soybean FLS. As a result, 68 molecular markers controlling soybean FLS were detected accurately, and the phenotypic contribution rate of these markers reached 82.45%. In this study, a model was established, which could be used directly to evaluate the resistance of soybean FLS and to select excellent offspring. This research method could also provide ideas and methods for other plants to breeding in disease resistance.展开更多
[Objectives]This study was conducted to clarify the species composition of pathogens causing leaf spot disease in yellow peach in Loudi area of Hunan Province and to screen effective control agents.[Methods]Twenty lea...[Objectives]This study was conducted to clarify the species composition of pathogens causing leaf spot disease in yellow peach in Loudi area of Hunan Province and to screen effective control agents.[Methods]Twenty leaf spot samples were collected from yellow peach planting bases in Lengshuijiang and Shuangjiang,Loudi City.Two isolates were obtained through tissue isolation and purification.Identification was performed based on morphological characteristics combined with rDNA-ITS and Bt-2a gene sequence analysis and pathogenicity assay.[Results]The pathogen causing yellow peach leaf spot in this area was Botryosphaeria dothidea.[Conclusions]This study provides a reference basis for the prevention and control of yellow peach leaf spot in Loudi area.展开更多
Arcopilus aureus is a plant-beneficial fungal species showing remarkable biocontrol capabilities.We investigated the antifungal activity of A.aureus YZXR strain and found that it strongly suppressed Polygonatum odorat...Arcopilus aureus is a plant-beneficial fungal species showing remarkable biocontrol capabilities.We investigated the antifungal activity of A.aureus YZXR strain and found that it strongly suppressed Polygonatum odoratum leaf spot disease caused by Fusarium fujikuroi.However,the antifungal substances and antifungal mechanisms remain unknown.To identify the antifungal substances,we extracted the fermentation liquid with ethyl acetate and found that the antifungal activity was mainly in the organic phase.Using column chromatography,semi-preparative HPLC,and GC–MS assays,we analyzed the compounds in the ethyl acetate phase and identified phenylethyl alcohol and 3,5-dihydroxytoluene as two compounds showing antifungal activity.The phenylethyl alcohol completely inhibited the growth of F.fujikuroi at a concentration of 0.25%(v/v),whereas the inhibition rate of 10.00 mg/mL 3,5-dihydroxytoluene on fungal growth was 80.60%.The fermentation broth of strain YZXR efficiently inhibited mycelial growth and conidiation of F.fujikuroi.Thus,this study uncovered the antifungal metabolites of A.aureus and the potential of A.aureus as a biological fungicide for managing P.odoratum leaf spot disease,providing a foundation for further research on its biocontrol mechanisms and field applications.展开更多
文摘Leaf spot disease seriously affects the yield and quality of waxberry, but there is no registration agent for prevention and control of the disease. The field trials showed that the control efficacy of 33.5% oxine-copper SC against leaf spot disease on waxberry reached above 74% when used at the dosage of 167.5-223.3 mg/kg. 33.5% Oxine-copper SC could be used to control leaf spot disease on waxberry.
基金High-level Talents Project of Hainan Provincial Natural Science Foundation of China(2019RC278)The Earmarked Fund for CARS(CARS-31)。
文摘[Objectives]The paper was to identify the causal agent of leaf spot of banana in Hainnan,China.[Methods]Fungus isolates were isolated from the diseased tissues,and morphological characteristics of colony and spore were observed.Furthermore,phylogenetic analyses and pathogenicity test were conducted to confirm the pathogen.[Results]The fungus isolates from the diseased tissues was identified as Curvularia geniculata.[Conclusions]C.geniculata is a new pathogen causing leaf spot of banana in Hainan,China.This finding will help to broaden the distribution and host range of C.geniculata,indicating that it poses potential damage to banana in China.
基金supported by Hainan Province key R&D Project(ZDYF2022XDNY242)the specific research fund of the Innovation Platform for Academicians of Hainan Province(YSPTZX202130).
文摘Robbsia andropogonis is one of the most destructive leaf spot disease pathogens of numerous host plants and causes heavy economic damage.In the present study,the complete genome of R.andropogonis strain BLB1,causing the leaf spot disease of areca palm,was generated using a hybrid method combining ONT PromethION long reads and BGISEQ-500 short reads.The resulting genome consists of seven replicons totaling 6,828,120 bp,and 5,808 genes were annotated,including 788 virulence-related genes.Function analysis showed that genes involved in metabolism were the most abundant group.Impressively,the bacteria were well-equipped with four,two,and four sets of type three,four,and six secretion systems,respectively,highlighting the virulence features of R.andropogonis BLB1.As the first complete genome sequence of the species of genus Robbsia,the BLB1 genome provides a solid foundation for investigation of mechanisms underlying the pathogen virulence and disease control,and will promote further discovery and characterization of the genus Robbsia.
文摘Aiming at the problem of low detection accuracy due to the different scale sizes of apple leaf disease spots and their similarity to the background,this paper proposes a multi-scale lightweight network(MSL-Net).Firstly,a multiplexed aggregated feature extraction network is proposed using residual bottleneck block(RES-Bottleneck)and middle partial-convolution(MP-Conv)to capture multi-scale spatial features and enhance focus on disease features for better differentiation between disease targets and background information.Secondly,a lightweight feature fusion network is designed using scale-fuse concatenation(SF-Cat)and triple-scale sequence feature fusion(TSSF)module to merge multi-scale feature maps comprehensively.Depthwise convolution(DWConv)and GhostNet lighten the network,while the cross stage partial bottleneck with 3 convolutions ghost-normalization attention module(C3-GN)reduces missed detections by suppressing irrelevant background information.Finally,soft non-maximum suppression(Soft-NMS)is used in the post-processing stage to improve the problem of misdetection of dense disease sites.The results show that the MSL-Net improves mean average precision at intersection over union of 0.5(mAP@0.5)by 2.0%over the baseline you only look once version 5s(YOLOv5s)and reduces parameters by 44%,reducing computation by 27%,outperforming other state-of-the-art(SOTA)models overall.This method also shows excellent performance compared to the latest research.
基金Supported by Huangshan Science and Technology Planning Project(2022KN-02)School-level Key Project of Huangshan University(2022XKJZD004)+2 种基金First-class Discipline in Huangshan University(YLXK202101)Key Project of Natural Science Foundation of Department of Education of Anhui Province(2022AH051953)Innovation Training Program(PX-135245491).
文摘[Objectives]This study was conducted to investigate the control of Chinese herbal medicine regulators on leaf spot nematode disease,the main pest of Chloranthus spicatus(Thunb.)Makino.[Methods]C.spicatus plants infected with nematodes were irrigated with a Chinese herbal medicine regulator at two different concentrations,and the control effect on leaf spot nematode disease of the plant was studied by measuring the number of nematodes,the contents of chlorophyll,malondialdehyde,soluble sugar and free proline in the leaves of the plants in the soil.[Results]Compared with the control,the two concentrations of Chinese herbal medicine regulator significantly reduced the number of nematodes in the soil,increased the contents of chlorophyll(mainly chlorophyll a),soluble sugar and free proline in the leaves of C.spicatus,and decreased the content of malondialdehyde.It indicated that the Chinese herbal medicine regulator could effectively control the spread of nematodes and had certain effect on the recovery of C.spicatus plants.[Conclusions]The use of Chinese herbal medicine regulators is helpful to curb the occurrence of leaf spot nematode disease in C.spicatus by biological prevention and control means,and provides theoretical guidance for the development of the green industry of C.spicatus.
基金Supported by High level Talents Project of Hainan Provincial Natural Science Foundation of China(2019RC278)The Earmarked Fund for CARS(CARS-31)。
文摘[Objectives]The paper was to study molecular characterization,morphology and pathogenicity of Curvularia pseudobrachyspora,a new causal agent of leaf spot on banana.[Methods]Banana(Musa acuminate)leaves with streaks or long ellipse-shaped lesions were sampled in an orchard of Danzhou City,Hainan Province,China in 2021.Fungus isolates were isolated from the diseased tissues and further identified as C.pseudobrachyspora based on morphological characteristics of colony,conidiophore and spore,phylogenetic analyses of the ITS region,GAPDH and TEF-1αgenes.[Results]In the pathogenicity test,the fungus re-isolated from inoculated leaves with necrotic lesions was identified morphologically and molecularly,fulfilling Koch's postulates.[Conclusions]C.pseudobrachyspora is a new pathogen causing leaf spot of banana in China and the world.
基金Hainan Provincial Natural Science Foundation of China(319MS081)Central Finance Forestry Science and Technology Extension Project(Qiong[2020]TG06).
文摘[Objectives]The study was to identify the causal agent of leaf spots of Camellia drupifera in Hainan Province,China.[Methods]Phylogenetic analyses,spore morphology and pathogenicity tests were adopted to identify the pathogen.[Results]The fungus was identified as Neofusicoccum parvum.The colonies were initially pale to white on PDA,with diffuse yellow pigment around the colonies in the agar after 5 d.After 10 d,the aerial mycelium became gray,and the substrate mycelium became black.Thirty days later,a large number of conidia were generated on OMA.Conidia were hyaline,nonseptate,and fusiform.The mean size of 100 conidia was(13.6-25.4)×(6.2-10.3)μm;the mean length/width ratio was(2.5±0.3)μm.[Conclusions]N.parvum was the causal agent of leaf spots of C.drupifera observed in Hainan Province,China.
基金Supported by the National Key Research and Development Program of China(2021YFD1201103-01-05)。
文摘Soybean frogeye leaf spot(FLS) disease is a global disease affecting soybean yield, especially in the soybean growing area of Heilongjiang Province. In order to realize genomic selection breeding for FLS resistance of soybean, least absolute shrinkage and selection operator(LASSO) regression and stepwise regression were combined, and a genomic selection model was established for 40 002 SNP markers covering soybean genome and relative lesion area of soybean FLS. As a result, 68 molecular markers controlling soybean FLS were detected accurately, and the phenotypic contribution rate of these markers reached 82.45%. In this study, a model was established, which could be used directly to evaluate the resistance of soybean FLS and to select excellent offspring. This research method could also provide ideas and methods for other plants to breeding in disease resistance.
基金Supported by Natural Science Foundation of Hunan Province(2024JJ72572025JJ70341)+1 种基金Research and Innovation Project of Hunan University of Humanities,Science and Technology(ZSCX2024Y11)Undergraduate Innovation and Entrepreneurship Training Program(S202410553043).
文摘[Objectives]This study was conducted to clarify the species composition of pathogens causing leaf spot disease in yellow peach in Loudi area of Hunan Province and to screen effective control agents.[Methods]Twenty leaf spot samples were collected from yellow peach planting bases in Lengshuijiang and Shuangjiang,Loudi City.Two isolates were obtained through tissue isolation and purification.Identification was performed based on morphological characteristics combined with rDNA-ITS and Bt-2a gene sequence analysis and pathogenicity assay.[Results]The pathogen causing yellow peach leaf spot in this area was Botryosphaeria dothidea.[Conclusions]This study provides a reference basis for the prevention and control of yellow peach leaf spot in Loudi area.
基金supported by the Changsha Natural Science Foundation of China(kq2208106)Research and application of early diagnosis and biological control of tobacco leaf spot disease(HYYC2023KJ34).
文摘Arcopilus aureus is a plant-beneficial fungal species showing remarkable biocontrol capabilities.We investigated the antifungal activity of A.aureus YZXR strain and found that it strongly suppressed Polygonatum odoratum leaf spot disease caused by Fusarium fujikuroi.However,the antifungal substances and antifungal mechanisms remain unknown.To identify the antifungal substances,we extracted the fermentation liquid with ethyl acetate and found that the antifungal activity was mainly in the organic phase.Using column chromatography,semi-preparative HPLC,and GC–MS assays,we analyzed the compounds in the ethyl acetate phase and identified phenylethyl alcohol and 3,5-dihydroxytoluene as two compounds showing antifungal activity.The phenylethyl alcohol completely inhibited the growth of F.fujikuroi at a concentration of 0.25%(v/v),whereas the inhibition rate of 10.00 mg/mL 3,5-dihydroxytoluene on fungal growth was 80.60%.The fermentation broth of strain YZXR efficiently inhibited mycelial growth and conidiation of F.fujikuroi.Thus,this study uncovered the antifungal metabolites of A.aureus and the potential of A.aureus as a biological fungicide for managing P.odoratum leaf spot disease,providing a foundation for further research on its biocontrol mechanisms and field applications.
