[Objective] The aim was to lay a foundation for research of contraceptive rodenticide with LDH-C4 as target protein. [ Method] EST sequence of LDH-C gene from black-lipped pika was cloned by PCR with degenerate primer...[Objective] The aim was to lay a foundation for research of contraceptive rodenticide with LDH-C4 as target protein. [ Method] EST sequence of LDH-C gene from black-lipped pika was cloned by PCR with degenerate primers; then the full length open reading frame （ORF） and 3＇UTR sequence were cloned by RACE technique. [ Result] The full length cDNA was 1 498 bp containing an ORF of 996 bp and a 3＇UTR of 486 bp. The ORF encoded a polypeptide of 332 amino acids. The alignment of LDH-C gene ORF nucleotide sequences from different species showed that the gene was conserved even between large taxons. The phylogenic tree showed that black-lipped pika LDH-C was closer to prima- tes and artiodactyla than to rodents. [Conclusion] cDNA sequence of LDH-C gene from black-lipped pika was cloned successfully.展开更多
Purified human LDH-C4was employed to immunize BALB/c mice. After fusion of the spleen cells from these immunized mice with myeloma, the hybridoma cells were
The paper reported the results of localization of LDH-C4 on sperm of various species by immunqftuorescent test. McAbs to mouse LDH-C4 which reacted not only with mouse sperm, but also with that of other species such a...The paper reported the results of localization of LDH-C4 on sperm of various species by immunqftuorescent test. McAbs to mouse LDH-C4 which reacted not only with mouse sperm, but also with that of other species such as rat, hamster, rabbit and human were used in this study. It is clear that LDH-C4 lacks species specificity. However, there exist different regions for localization of LDH-C4 on sperm of various species, It is suggested that gametes in various species may also differ in their LDH-C4 surface expression. The present study also showed that these McAbs to mouse LDH-C4 have different binding capacity to sperm qf varlous species. The result that LDH-C4 was mainly localized on midpiece of mouse sperm in our experiments is in discrepancy with those previously reported by other investigators.展开更多
目的分析甲泼尼龙琥珀酸钠辅助治疗儿童重症肺炎支原体肺炎(SMPP)的临床疗效及对患儿血乳酸脱氢酶(LDH)、C反应蛋白(CRP)和T淋巴细胞亚群的影响。方法选取2023年1月—2024年1月于河北医科大学第二医院儿科收治的120例重症支原体肺炎患...目的分析甲泼尼龙琥珀酸钠辅助治疗儿童重症肺炎支原体肺炎(SMPP)的临床疗效及对患儿血乳酸脱氢酶(LDH)、C反应蛋白(CRP)和T淋巴细胞亚群的影响。方法选取2023年1月—2024年1月于河北医科大学第二医院儿科收治的120例重症支原体肺炎患儿为研究对象。其中治疗组(常规治疗基础上加上甲泼尼龙琥珀酸钠)80例,其中使用甲泼尼龙琥珀酸钠3 d 40例(短疗程组),使用5 d 40例(延长组),对照组(常规治疗)40例。通过两两比较患儿体温正常、咳嗽消失及啰音消失时间,治疗前后CRP及LDH改善情况进行综合分析。结果与对照组相比,治疗组患儿体温正常、啰音消失、咳嗽消失的时间更短,两者比较差异有统计学意义;治疗组中短疗程组与延长组比较,两组患儿体温正常、啰音消失、咳嗽消失的时间差异均无统计学意义。在炎症指标方面(CRP和LDH),两组患儿治疗前比较差异均无统计学意义,治疗后治疗组改善情况优于对照组,两者比较差异均有统计学意义;其中治疗组两者比较,治疗前后CRP及LDH改善情况差异均无统计学意义。在T淋巴细胞亚群的比较中,与对照组相比,治疗组CD4^(+)及CD8^(+)的改善更明显,两者比较差异均有统计学意义;治疗组中短疗程组与延长组比较,两组患儿CD4^(+)及CD8^(+)的改善均无统计学意义。结论甲泼尼龙琥珀酸钠辅助治疗儿童SMPP临床疗效显著,可以缩短患儿体温正常、咳嗽消失及啰音消失时间,还可以明显改善炎症指标(CRP及LDH)恢复情况,从而减少患儿痛苦,缩短住院时间。展开更多
基金Supported by State Ethnic Affairs Commission of P.R.C.(08XN04)Applicable and Fundamental Research Funds of SichuanProvince(2008JY0068)Academic Culture and TechnologyLeaders in Sichuan Province Foundation~~
文摘[Objective] The aim was to lay a foundation for research of contraceptive rodenticide with LDH-C4 as target protein. [ Method] EST sequence of LDH-C gene from black-lipped pika was cloned by PCR with degenerate primers; then the full length open reading frame （ORF） and 3＇UTR sequence were cloned by RACE technique. [ Result] The full length cDNA was 1 498 bp containing an ORF of 996 bp and a 3＇UTR of 486 bp. The ORF encoded a polypeptide of 332 amino acids. The alignment of LDH-C gene ORF nucleotide sequences from different species showed that the gene was conserved even between large taxons. The phylogenic tree showed that black-lipped pika LDH-C was closer to prima- tes and artiodactyla than to rodents. [Conclusion] cDNA sequence of LDH-C gene from black-lipped pika was cloned successfully.
文摘Purified human LDH-C4was employed to immunize BALB/c mice. After fusion of the spleen cells from these immunized mice with myeloma, the hybridoma cells were
文摘The paper reported the results of localization of LDH-C4 on sperm of various species by immunqftuorescent test. McAbs to mouse LDH-C4 which reacted not only with mouse sperm, but also with that of other species such as rat, hamster, rabbit and human were used in this study. It is clear that LDH-C4 lacks species specificity. However, there exist different regions for localization of LDH-C4 on sperm of various species, It is suggested that gametes in various species may also differ in their LDH-C4 surface expression. The present study also showed that these McAbs to mouse LDH-C4 have different binding capacity to sperm qf varlous species. The result that LDH-C4 was mainly localized on midpiece of mouse sperm in our experiments is in discrepancy with those previously reported by other investigators.
文摘目的分析甲泼尼龙琥珀酸钠辅助治疗儿童重症肺炎支原体肺炎(SMPP)的临床疗效及对患儿血乳酸脱氢酶(LDH)、C反应蛋白(CRP)和T淋巴细胞亚群的影响。方法选取2023年1月—2024年1月于河北医科大学第二医院儿科收治的120例重症支原体肺炎患儿为研究对象。其中治疗组(常规治疗基础上加上甲泼尼龙琥珀酸钠)80例,其中使用甲泼尼龙琥珀酸钠3 d 40例(短疗程组),使用5 d 40例(延长组),对照组(常规治疗)40例。通过两两比较患儿体温正常、咳嗽消失及啰音消失时间,治疗前后CRP及LDH改善情况进行综合分析。结果与对照组相比,治疗组患儿体温正常、啰音消失、咳嗽消失的时间更短,两者比较差异有统计学意义;治疗组中短疗程组与延长组比较,两组患儿体温正常、啰音消失、咳嗽消失的时间差异均无统计学意义。在炎症指标方面(CRP和LDH),两组患儿治疗前比较差异均无统计学意义,治疗后治疗组改善情况优于对照组,两者比较差异均有统计学意义;其中治疗组两者比较,治疗前后CRP及LDH改善情况差异均无统计学意义。在T淋巴细胞亚群的比较中,与对照组相比,治疗组CD4^(+)及CD8^(+)的改善更明显,两者比较差异均有统计学意义;治疗组中短疗程组与延长组比较,两组患儿CD4^(+)及CD8^(+)的改善均无统计学意义。结论甲泼尼龙琥珀酸钠辅助治疗儿童SMPP临床疗效显著,可以缩短患儿体温正常、咳嗽消失及啰音消失时间,还可以明显改善炎症指标(CRP及LDH)恢复情况,从而减少患儿痛苦,缩短住院时间。
