Phenols,flavonoids,and anthocyanins are compounds that reported play important roles in plant defence mechanism system.Antioxidant activity is mostly derived from these phytochemical groups,which have a wide range of ...Phenols,flavonoids,and anthocyanins are compounds that reported play important roles in plant defence mechanism system.Antioxidant activity is mostly derived from these phytochemical groups,which have a wide range of pharmaceutical properties,including antimicrobial,antitumor,anti-inflammatory,antipyretic,antiallergy,anti-fertility,hypoglycemic,anti-histamine,antioxidant,antiviral,analgesic,anti-ulcerogenic,neph-roprotective,antidiabetic,immuno-secretory,cardiovascular,anti-platelet aggregation,antibacterial,anticancer,and hepatoprotective activities.However,these compounds in Solanum lasiocarpum from Borneo areas are remaining unclear.In this experiment,we performed comprehensive evaluation on effect of extraction solvents on phenols,flavonoids,and anthocyanin’s contents and further on the scavenging activity(2,2-diphenyl-1-pic-rylhydrazyl-hydrate,DPPH;2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid),ABTS;and ferric-reducing antioxidant power,FRAP).High phenolic,flavonoid and anthocyanin contents be found in low polar solvent.Antioxidant assay showed acetone was higher activity with IC_(50) of 6.36 mg/mL compared to methanol(12.1 mg/mL)and hexane(17.8 mg/mL).Furthermore,liquid chromatography mass spectrometry quadrupole-time of flight(LCMS Q-TOF)mass spectrometer detected N-Acryloylglycine,butoctamide hydrogen succinate,eruberin B,emmotin A and N-Cyclohexanecarbonylpentadecylamine that important in natural polymers,serve as sleep disorders treatment agents,anti-fibrotic property,immunomodulatory activity,and act as differentiator between N-palmitoylethanolamine-hydrolyzing acid amidase(NPAA)compared to fatty acid amide hydrolase(FAAH),respectively.The limitation of this research is the assessment of antioxidant properties related to the polarity of the extraction solvent.Polar solvents may exhibit high scavenging activity,but phenolic compounds,flavonoids,and anthocyanins are found in higher concentrations in non-polar solvents.We encourage further investigation into phytochemical profiles due to the abundance from non-polar solvents.展开更多
Leaf color mutants (LCMs) provide crucial insights into the regulatory mechanisms underlying chloroplast development,photo synthesis,and stre ss adaptation.In this study,we identified a temperature-sensitive albino mu...Leaf color mutants (LCMs) provide crucial insights into the regulatory mechanisms underlying chloroplast development,photo synthesis,and stre ss adaptation.In this study,we identified a temperature-sensitive albino mutant,tsa4,characterized by an albino phenotype at the seedling stage and abnormal chloroplast development at temperatures below 25℃.展开更多
Lost circulation of drilling fluid is one of the most common and costly problems in drilling operations.This highlights the importance of wellbore strengthening treatment sthat can utilize lost circulation materials(L...Lost circulation of drilling fluid is one of the most common and costly problems in drilling operations.This highlights the importance of wellbore strengthening treatment sthat can utilize lost circulation materials(LCMs)to seal fractures associated with the wellbore.In this work,a numerical model accounting for the deformation of surrounding rock,fluid flow in the fracture,fracture propagation,and the transport of LCMs is presented to investigate the wellbore strengthening,from the fracture initiation to the fracture arrest,due to plugs formed by LCMs.The equations governing the rock deformation and fluid flow are solved by the dual boundary element method and the finite volume method,respectively.The transport of LCMs is solved based on an empirical constitutive model in suspension flow,and several characteristic quantities are derived by dimensional analysis.It is found that two dimensionless parameters,dimensionless toughness and normalized initial particle concentration,control the migration of LCM particles.The numerical results show that the dimensionless toughness influences the entrance and bridging of LCMs while the initial concentration controls the location of the particle bridging.When the initial concentration is larger than 0.8,the particle bridging tends to occur near the fracture entry.Conversely,when the initial concentration is less than 0.8,the particle bridging occurs near the fracture tip.This work provides an effective tool to predict the LCM transport and plugging in the wellbore strengthening process.展开更多
基金funded by Universiti Malaysia Sabah and project code numbers were DN20082 and GUG0619.
文摘Phenols,flavonoids,and anthocyanins are compounds that reported play important roles in plant defence mechanism system.Antioxidant activity is mostly derived from these phytochemical groups,which have a wide range of pharmaceutical properties,including antimicrobial,antitumor,anti-inflammatory,antipyretic,antiallergy,anti-fertility,hypoglycemic,anti-histamine,antioxidant,antiviral,analgesic,anti-ulcerogenic,neph-roprotective,antidiabetic,immuno-secretory,cardiovascular,anti-platelet aggregation,antibacterial,anticancer,and hepatoprotective activities.However,these compounds in Solanum lasiocarpum from Borneo areas are remaining unclear.In this experiment,we performed comprehensive evaluation on effect of extraction solvents on phenols,flavonoids,and anthocyanin’s contents and further on the scavenging activity(2,2-diphenyl-1-pic-rylhydrazyl-hydrate,DPPH;2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid),ABTS;and ferric-reducing antioxidant power,FRAP).High phenolic,flavonoid and anthocyanin contents be found in low polar solvent.Antioxidant assay showed acetone was higher activity with IC_(50) of 6.36 mg/mL compared to methanol(12.1 mg/mL)and hexane(17.8 mg/mL).Furthermore,liquid chromatography mass spectrometry quadrupole-time of flight(LCMS Q-TOF)mass spectrometer detected N-Acryloylglycine,butoctamide hydrogen succinate,eruberin B,emmotin A and N-Cyclohexanecarbonylpentadecylamine that important in natural polymers,serve as sleep disorders treatment agents,anti-fibrotic property,immunomodulatory activity,and act as differentiator between N-palmitoylethanolamine-hydrolyzing acid amidase(NPAA)compared to fatty acid amide hydrolase(FAAH),respectively.The limitation of this research is the assessment of antioxidant properties related to the polarity of the extraction solvent.Polar solvents may exhibit high scavenging activity,but phenolic compounds,flavonoids,and anthocyanins are found in higher concentrations in non-polar solvents.We encourage further investigation into phytochemical profiles due to the abundance from non-polar solvents.
基金financially supported by the National Natural Science Foundation of China(Grant Nos.32341026 and 32171998)the Hunan Provincial Science and Technology Innovation Program,China(Grant No.2023NK1010)the Changsha Natural Science Foundation,China(Grant No.20209001).
文摘Leaf color mutants (LCMs) provide crucial insights into the regulatory mechanisms underlying chloroplast development,photo synthesis,and stre ss adaptation.In this study,we identified a temperature-sensitive albino mutant,tsa4,characterized by an albino phenotype at the seedling stage and abnormal chloroplast development at temperatures below 25℃.
基金support of the National Natural Science Foundation of China(Grant No.52371279)the Program for International Exchange and Cooperation in Education by the Ministry of Education of the People's Republic of China(Grant No.57220500123).
文摘Lost circulation of drilling fluid is one of the most common and costly problems in drilling operations.This highlights the importance of wellbore strengthening treatment sthat can utilize lost circulation materials(LCMs)to seal fractures associated with the wellbore.In this work,a numerical model accounting for the deformation of surrounding rock,fluid flow in the fracture,fracture propagation,and the transport of LCMs is presented to investigate the wellbore strengthening,from the fracture initiation to the fracture arrest,due to plugs formed by LCMs.The equations governing the rock deformation and fluid flow are solved by the dual boundary element method and the finite volume method,respectively.The transport of LCMs is solved based on an empirical constitutive model in suspension flow,and several characteristic quantities are derived by dimensional analysis.It is found that two dimensionless parameters,dimensionless toughness and normalized initial particle concentration,control the migration of LCM particles.The numerical results show that the dimensionless toughness influences the entrance and bridging of LCMs while the initial concentration controls the location of the particle bridging.When the initial concentration is larger than 0.8,the particle bridging tends to occur near the fracture entry.Conversely,when the initial concentration is less than 0.8,the particle bridging occurs near the fracture tip.This work provides an effective tool to predict the LCM transport and plugging in the wellbore strengthening process.
