The pyrrolizidine alkaloids(PAs) in leaf of Gynura divaricata(L.) DC.was analyzed by LC-MSn technique.The result shows that two hepatotoxic pyrrolizidine alkaloids(HPAs) are detected from the methanol extract of G.div...The pyrrolizidine alkaloids(PAs) in leaf of Gynura divaricata(L.) DC.was analyzed by LC-MSn technique.The result shows that two hepatotoxic pyrrolizidine alkaloids(HPAs) are detected from the methanol extract of G.divaricata leaf,with relative molecular weight of 335 and 365,respectively and lower content.The constituent with relative molecular weight 335 is a retronecine-type alkaloid,and identified as integerrimine;another constituent with relative molecular weight 365 is an otonecine-type alkaloid,speculated to be isomer of usaramine or 18-hydroxyinterrimine.展开更多
目的:对药物利培酮进行微生物转化研究,并对主要转化产物进行检识和结构鉴定,预测其微生物转化途径。方法:构建利培酮微生物转化模型,采用LC-MSn方法检测原药及其转化物;通过比较菌液样品和空白样品的正离子色谱图寻找转化产物,根据其...目的:对药物利培酮进行微生物转化研究,并对主要转化产物进行检识和结构鉴定,预测其微生物转化途径。方法:构建利培酮微生物转化模型,采用LC-MSn方法检测原药及其转化物;通过比较菌液样品和空白样品的正离子色谱图寻找转化产物,根据其多级质谱图,确定转化产物的分子结构,进而预测利培酮微生物转化途径。结果:从不同培养时间利培酮微生物转化液中鉴定出4个转化产物,除产物9-OH利培酮外,其余3个转化产物可能都是新的利培酮代谢产物。结论:利培酮在Cunninghamella blakesleeana AS 3.153中被转化为多种产物,利用LC-MSn可以快速检查和鉴定。展开更多
基金Scholarship for Stabilizing Tablent from Hunan Agricultural University (05WD11)Scholarslip for Excellent Youthfrom Hunan Education Department (07B035)
文摘The pyrrolizidine alkaloids(PAs) in leaf of Gynura divaricata(L.) DC.was analyzed by LC-MSn technique.The result shows that two hepatotoxic pyrrolizidine alkaloids(HPAs) are detected from the methanol extract of G.divaricata leaf,with relative molecular weight of 335 and 365,respectively and lower content.The constituent with relative molecular weight 335 is a retronecine-type alkaloid,and identified as integerrimine;another constituent with relative molecular weight 365 is an otonecine-type alkaloid,speculated to be isomer of usaramine or 18-hydroxyinterrimine.
文摘目的:对药物利培酮进行微生物转化研究,并对主要转化产物进行检识和结构鉴定,预测其微生物转化途径。方法:构建利培酮微生物转化模型,采用LC-MSn方法检测原药及其转化物;通过比较菌液样品和空白样品的正离子色谱图寻找转化产物,根据其多级质谱图,确定转化产物的分子结构,进而预测利培酮微生物转化途径。结果:从不同培养时间利培酮微生物转化液中鉴定出4个转化产物,除产物9-OH利培酮外,其余3个转化产物可能都是新的利培酮代谢产物。结论:利培酮在Cunninghamella blakesleeana AS 3.153中被转化为多种产物,利用LC-MSn可以快速检查和鉴定。
