Objective:To screen potential serum differential proteins in insulin resistance(IR)complicated with polycystic ovary syndrome(PCOS)using isobaric tags for relative and absolute quantitation(i TRAQ)combined with ultra-...Objective:To screen potential serum differential proteins in insulin resistance(IR)complicated with polycystic ovary syndrome(PCOS)using isobaric tags for relative and absolute quantitation(i TRAQ)combined with ultra-high performance liquid chromatography-tandem mass spectrometry(LC-MS/MS).Methods:A total of 20 patients diagnosed with PCOS in our hospital were selected,including 10 cases of simple PCOS and 10 cases of PCOS+IR.i TRAQ combined with LC-MS/MS was used for proteomic analysis to identify serum differential proteins.Bioinformatics analyses,including gene ontology(GO),Kyoto encyclopedia of genes and genomes(KEGG),and protein-protein interaction(PPI)analysis,were conducted to understand the biological processes,cellular components,and molecular functions of the differentially expressed proteins detected by the two methods.Results:A total of 454,675 secondary spectra were detected by iTRAQ,with 14,376 matched spectra.Combined with LC-MS/MS,74,386peptides,47,542 unique peptide sequences,and 54,675 proteins were identified.A total of 249 differentially expressed proteins with a fold change≥1.30 or≤0.83 were found,among which 9 had a P-value<0.05.There were 5 up-regulated and 4 down-regulated proteins,namely RPAP3,ALDH6A1,COX20,RASSF3,ALPK2,NANOS1,FAM210A,CHGA,and CGA.These differential proteins were imported into the STRING database for PPI network analysis,which revealed 21 nodes and 69 edges with a PPI enrichment P-value<0.001.KEGG enrichment results included hsa04913(Ovarian steroidogenesis),hsa04024(cAMP signaling pathway),and hsa04080(Neuroactive ligand-receptor interaction).Conclusion:The combination of i TRAQ and LC-MS/MS technologies identified nine differential proteins including CHGA and CGA,which are mainly enriched in pathways related to ovarian steroidogenesis,c AMP signaling,and neuroactive ligand-receptor interaction.This combination provides a powerful platform for exploring the pathogenesis of IR complicated with PCOS and discovering new biomarkers.展开更多
目的对红花注射液进行化学成分分析及应用分子对接技术筛选红花注射液中过敏原成分。方法应用高效液相色谱-离子阱-飞行时间质谱高分辨质谱技术(high performance liquid chromatography-ion trap-time-of-flight high resolution mass ...目的对红花注射液进行化学成分分析及应用分子对接技术筛选红花注射液中过敏原成分。方法应用高效液相色谱-离子阱-飞行时间质谱高分辨质谱技术(high performance liquid chromatography-ion trap-time-of-flight high resolution mass spectrometry,HPLC-IT-TOF-MS)鉴定红花注射液中化学成分,应用分子对接技术进一步对潜在过敏原成分进行筛选。结果从红花注射液中共鉴定出40个化合物,其中26个查耳酮碳苷类化合物、8个黄酮类化合物、3个生物碱类化合物和3个其他类化合物,所有化合物均在负离子模式下鉴定。将这些化合物分别与人血清白蛋白(human serum albumin,HSA)、Mas相关G蛋白偶联受体X2(Mas-related G protein-coupled receptor X2,MRGPRX2)、Ras同源基因家族成员A(Ras homolog gene family member A)等蛋白进行分子对接,其中与HAS结合的化合物6个,与MRGPRX2结合的化合物有9个,发现红花注射液中过敏成分主要为6-羟基山柰酚类化合物和红花醌类化合物。结论建立了液-质联用与分子对接相结合的过敏原筛选方法,从红花注射液中鉴定出40个化合物,明确6-羟基山柰酚类和红花醌类化合物为潜在过敏原,为红花注射液过敏原筛选及其致敏机制研究提供了重要参考和数据。展开更多
With the accelerating aging process of China’s population,the demand for community elderly care services has shown diversified and personalized characteristics.However,problems such as insufficient total care service...With the accelerating aging process of China’s population,the demand for community elderly care services has shown diversified and personalized characteristics.However,problems such as insufficient total care service resources,uneven distribution,and prominent supply-demand contradictions have seriously affected service quality.Big data technology,with core advantages including data collection,analysis and mining,and accurate prediction,provides a new solution for the allocation of community elderly care service resources.This paper systematically studies the application value of big data technology in the allocation of community elderly care service resources from three aspects:resource allocation efficiency,service accuracy,and management intelligence.Combined with practical needs,it proposes optimal allocation strategies such as building a big data analysis platform and accurately grasping the elderly’s care needs,striving to provide operable path references for the construction of community elderly care service systems,promoting the early realization of the elderly care service goal of“adequate support and proper care for the elderly”,and boosting the high-quality development of China’s elderly care service industry.展开更多
In response to the Ministry of Education’s requirements for building distinctive model software schools,Software College of Northeastern University and Shenzhen Kingdom Technology Co.,Ltd.jointly developed the specia...In response to the Ministry of Education’s requirements for building distinctive model software schools,Software College of Northeastern University and Shenzhen Kingdom Technology Co.,Ltd.jointly developed the specialized course“Application and Practice of RPA Technology in FinTech”.Addressing pain points in financial digital transformation,the course integrates robotic process automation(RPA)principles,financial domain knowledge,and RPA platform practice into a“technology-scenario-capability”trinity teaching system.Through 64 credit hours of integrated theory and practice,it covers RPA fundamentals,financial applications,RPA operations(including core skills like Web/desktop automation),and AI integration,cultivating students’ability to design and implement automated financial workflows.It innovatively features a RPA simulation platform,30+financial case studies,and modular task resources,creating a“teacher-machine-student”interactive model.Practice demonstrates the course effectively enhances students’integration of technical application and business acumen,providing a scalable paradigm for cultivating interdisciplinary FinTech talent.展开更多
The moment a media delegation from the Republic of the Congo arrived at the Othello Kitchenware Museum on 18 November 2025,they were greeted with a vivid show of Guangdong’s industrial strength.Standing before them w...The moment a media delegation from the Republic of the Congo arrived at the Othello Kitchenware Museum on 18 November 2025,they were greeted with a vivid show of Guangdong’s industrial strength.Standing before them was not a typical exhibition hall,but a building shaped like a gleaming stainless-steel cooking pot.展开更多
Jeanologia celebrated its 30th anniversary reaffirming the mission it was born with in 1994,to transform the textile industry into a more sustainable,efficient,and human-centered sector.Three decades later,that vision...Jeanologia celebrated its 30th anniversary reaffirming the mission it was born with in 1994,to transform the textile industry into a more sustainable,efficient,and human-centered sector.Three decades later,that vision has become a global reality.Today,more than 40 percent of all jeans produced worldwide are made using technologies developed by the Valencia-based company.What started in a small laboratory in Valencia(Spain),with a pioneering team and a visionary idea,has evolved into a global benchmark in sustainable innovation.From the beginning,Jeanologia believed in technology as a driver of change,introducing solutions that eliminated harmful practices and opened the door to a new,cleaner way of making garments.展开更多
Rare earth(RE)Y-type zeolite was synthesized in situ by acidic co-hydrolysis route and hydrothermal method.The key process parameters were optimized based on the RE utilization rate.The effect of inducing a rotating p...Rare earth(RE)Y-type zeolite was synthesized in situ by acidic co-hydrolysis route and hydrothermal method.The key process parameters were optimized based on the RE utilization rate.The effect of inducing a rotating packed bed(RPB)in premixing and crystallization on crystallinity and RE utilization rate was further investigated.The results indicate that lanthanide(La)cations are successfully introduced into the sodalite cage of Y-type zeolite.The optimized conditions are that the molar ratio of Si/La is 150,premixing for 5 h,crystallization at 90℃ for 18 h,and calcination at 550℃ for 3.5 h.At this stage,the RE utilization rate reaches 74.5%.Compared with the conventional stirred tank reactor(STR),RPB can effectively shorten the premixing time and crystallization time by 4.3 h and 6 h,improve the crystallinity by 23%and RE utilization rate by 7.5%.The RE utilization rate is more than 80%by RPB,surpassing the effectiveness of using the one-exchange one-calcination process in the traditional liquid ion exchange process.It is expected to provide a reference for the in-situ efficient and green synthesis of RE zeolite.展开更多
Aiming at the problems of lagging curriculum,weak practice,and single evaluation in the cultivation of HarmonyOS Development talents,this study constructs a“teacher-machine-student”ternary interactive teaching model...Aiming at the problems of lagging curriculum,weak practice,and single evaluation in the cultivation of HarmonyOS Development talents,this study constructs a“teacher-machine-student”ternary interactive teaching model based on the Congyou platform.Through the building block curriculum system,the HarmonyOS technology stack is decoupled into dynamic capability units,and a multi-disciplinary cross-case library is jointly built with Huawei,which significantly improves the synchronization of teaching content and industrial technology.This paper innovatively designs an AI collaborative teaching system,which employs knowledge graphs to plan learning paths,utilizes virtual equipment clusters to simulate development environments,and establishes a“diagnosis-feedback-enhancement”closed loop through AI-based review,thereby effectively improving students’development efficiency and code reuse rate.A three-dimensional evaluation model integrating task outcomes,process performance,and innovation is constructed,incorporating indicators such as code standardization and an innovation index to strengthen the cultivation of engineering thinking and innovative ability.Furthermore,a data-driven support platform is built to generate student competency profiles,open up the“credit-competency-certification”pathway,promote the transformation of course achievements into contributions to the Huawei ecosystem,and significantly shorten the job adaptation cycle for graduates.The research results provide a replicable paradigm for the cultivation of domestic operating system talents.展开更多
文摘Objective:To screen potential serum differential proteins in insulin resistance(IR)complicated with polycystic ovary syndrome(PCOS)using isobaric tags for relative and absolute quantitation(i TRAQ)combined with ultra-high performance liquid chromatography-tandem mass spectrometry(LC-MS/MS).Methods:A total of 20 patients diagnosed with PCOS in our hospital were selected,including 10 cases of simple PCOS and 10 cases of PCOS+IR.i TRAQ combined with LC-MS/MS was used for proteomic analysis to identify serum differential proteins.Bioinformatics analyses,including gene ontology(GO),Kyoto encyclopedia of genes and genomes(KEGG),and protein-protein interaction(PPI)analysis,were conducted to understand the biological processes,cellular components,and molecular functions of the differentially expressed proteins detected by the two methods.Results:A total of 454,675 secondary spectra were detected by iTRAQ,with 14,376 matched spectra.Combined with LC-MS/MS,74,386peptides,47,542 unique peptide sequences,and 54,675 proteins were identified.A total of 249 differentially expressed proteins with a fold change≥1.30 or≤0.83 were found,among which 9 had a P-value<0.05.There were 5 up-regulated and 4 down-regulated proteins,namely RPAP3,ALDH6A1,COX20,RASSF3,ALPK2,NANOS1,FAM210A,CHGA,and CGA.These differential proteins were imported into the STRING database for PPI network analysis,which revealed 21 nodes and 69 edges with a PPI enrichment P-value<0.001.KEGG enrichment results included hsa04913(Ovarian steroidogenesis),hsa04024(cAMP signaling pathway),and hsa04080(Neuroactive ligand-receptor interaction).Conclusion:The combination of i TRAQ and LC-MS/MS technologies identified nine differential proteins including CHGA and CGA,which are mainly enriched in pathways related to ovarian steroidogenesis,c AMP signaling,and neuroactive ligand-receptor interaction.This combination provides a powerful platform for exploring the pathogenesis of IR complicated with PCOS and discovering new biomarkers.
文摘目的对红花注射液进行化学成分分析及应用分子对接技术筛选红花注射液中过敏原成分。方法应用高效液相色谱-离子阱-飞行时间质谱高分辨质谱技术(high performance liquid chromatography-ion trap-time-of-flight high resolution mass spectrometry,HPLC-IT-TOF-MS)鉴定红花注射液中化学成分,应用分子对接技术进一步对潜在过敏原成分进行筛选。结果从红花注射液中共鉴定出40个化合物,其中26个查耳酮碳苷类化合物、8个黄酮类化合物、3个生物碱类化合物和3个其他类化合物,所有化合物均在负离子模式下鉴定。将这些化合物分别与人血清白蛋白(human serum albumin,HSA)、Mas相关G蛋白偶联受体X2(Mas-related G protein-coupled receptor X2,MRGPRX2)、Ras同源基因家族成员A(Ras homolog gene family member A)等蛋白进行分子对接,其中与HAS结合的化合物6个,与MRGPRX2结合的化合物有9个,发现红花注射液中过敏成分主要为6-羟基山柰酚类化合物和红花醌类化合物。结论建立了液-质联用与分子对接相结合的过敏原筛选方法,从红花注射液中鉴定出40个化合物,明确6-羟基山柰酚类和红花醌类化合物为潜在过敏原,为红花注射液过敏原筛选及其致敏机制研究提供了重要参考和数据。
文摘With the accelerating aging process of China’s population,the demand for community elderly care services has shown diversified and personalized characteristics.However,problems such as insufficient total care service resources,uneven distribution,and prominent supply-demand contradictions have seriously affected service quality.Big data technology,with core advantages including data collection,analysis and mining,and accurate prediction,provides a new solution for the allocation of community elderly care service resources.This paper systematically studies the application value of big data technology in the allocation of community elderly care service resources from three aspects:resource allocation efficiency,service accuracy,and management intelligence.Combined with practical needs,it proposes optimal allocation strategies such as building a big data analysis platform and accurately grasping the elderly’s care needs,striving to provide operable path references for the construction of community elderly care service systems,promoting the early realization of the elderly care service goal of“adequate support and proper care for the elderly”,and boosting the high-quality development of China’s elderly care service industry.
文摘In response to the Ministry of Education’s requirements for building distinctive model software schools,Software College of Northeastern University and Shenzhen Kingdom Technology Co.,Ltd.jointly developed the specialized course“Application and Practice of RPA Technology in FinTech”.Addressing pain points in financial digital transformation,the course integrates robotic process automation(RPA)principles,financial domain knowledge,and RPA platform practice into a“technology-scenario-capability”trinity teaching system.Through 64 credit hours of integrated theory and practice,it covers RPA fundamentals,financial applications,RPA operations(including core skills like Web/desktop automation),and AI integration,cultivating students’ability to design and implement automated financial workflows.It innovatively features a RPA simulation platform,30+financial case studies,and modular task resources,creating a“teacher-machine-student”interactive model.Practice demonstrates the course effectively enhances students’integration of technical application and business acumen,providing a scalable paradigm for cultivating interdisciplinary FinTech talent.
文摘The moment a media delegation from the Republic of the Congo arrived at the Othello Kitchenware Museum on 18 November 2025,they were greeted with a vivid show of Guangdong’s industrial strength.Standing before them was not a typical exhibition hall,but a building shaped like a gleaming stainless-steel cooking pot.
文摘Jeanologia celebrated its 30th anniversary reaffirming the mission it was born with in 1994,to transform the textile industry into a more sustainable,efficient,and human-centered sector.Three decades later,that vision has become a global reality.Today,more than 40 percent of all jeans produced worldwide are made using technologies developed by the Valencia-based company.What started in a small laboratory in Valencia(Spain),with a pioneering team and a visionary idea,has evolved into a global benchmark in sustainable innovation.From the beginning,Jeanologia believed in technology as a driver of change,introducing solutions that eliminated harmful practices and opened the door to a new,cleaner way of making garments.
基金supported by the NationalKey Research and Development Program of China(2023YFA1507701)National Natural Science Foundation of China(U22B6011,22288102)“Announcement and Challenge”Science and Technology Project of Xinjiang Uygur Autonomous Region(XJKJTJBGS-2023).
文摘Rare earth(RE)Y-type zeolite was synthesized in situ by acidic co-hydrolysis route and hydrothermal method.The key process parameters were optimized based on the RE utilization rate.The effect of inducing a rotating packed bed(RPB)in premixing and crystallization on crystallinity and RE utilization rate was further investigated.The results indicate that lanthanide(La)cations are successfully introduced into the sodalite cage of Y-type zeolite.The optimized conditions are that the molar ratio of Si/La is 150,premixing for 5 h,crystallization at 90℃ for 18 h,and calcination at 550℃ for 3.5 h.At this stage,the RE utilization rate reaches 74.5%.Compared with the conventional stirred tank reactor(STR),RPB can effectively shorten the premixing time and crystallization time by 4.3 h and 6 h,improve the crystallinity by 23%and RE utilization rate by 7.5%.The RE utilization rate is more than 80%by RPB,surpassing the effectiveness of using the one-exchange one-calcination process in the traditional liquid ion exchange process.It is expected to provide a reference for the in-situ efficient and green synthesis of RE zeolite.
文摘Aiming at the problems of lagging curriculum,weak practice,and single evaluation in the cultivation of HarmonyOS Development talents,this study constructs a“teacher-machine-student”ternary interactive teaching model based on the Congyou platform.Through the building block curriculum system,the HarmonyOS technology stack is decoupled into dynamic capability units,and a multi-disciplinary cross-case library is jointly built with Huawei,which significantly improves the synchronization of teaching content and industrial technology.This paper innovatively designs an AI collaborative teaching system,which employs knowledge graphs to plan learning paths,utilizes virtual equipment clusters to simulate development environments,and establishes a“diagnosis-feedback-enhancement”closed loop through AI-based review,thereby effectively improving students’development efficiency and code reuse rate.A three-dimensional evaluation model integrating task outcomes,process performance,and innovation is constructed,incorporating indicators such as code standardization and an innovation index to strengthen the cultivation of engineering thinking and innovative ability.Furthermore,a data-driven support platform is built to generate student competency profiles,open up the“credit-competency-certification”pathway,promote the transformation of course achievements into contributions to the Huawei ecosystem,and significantly shorten the job adaptation cycle for graduates.The research results provide a replicable paradigm for the cultivation of domestic operating system talents.
