For the first time, mass spectrometric (MS) techniques were employed to rapidly detect the pathogen Chalara fraxinea in-vitro and directly in-vivo in tissues of diseased ash trees caused by C. fraxinea, using a range ...For the first time, mass spectrometric (MS) techniques were employed to rapidly detect the pathogen Chalara fraxinea in-vitro and directly in-vivo in tissues of diseased ash trees caused by C. fraxinea, using a range of characteristic novel secondary metabolites of C. fraxinea as chemical markers for the presence of the pathogen. We have found an evident correlation between the presence and amount of these-only for C. fraxinea characteristic and novel-secondary metabolites (named chalarafraxinines) and the degree of disease of respective infected ash seedlings. As demonstrated in this work, the MS based high-throughput-screening approach constitute an alternative to the time consuming and expensive micro biological isolation procedures for detection of the pathogen C. fraxinea and furthermore, can be used to rapidly test ash genotypes for resistance / susceptibility to C. fraxinea infection.展开更多
MALDI-TOF-MS technology was used for identification of lipopeptide antibiotics producedby GEB3 strain, a derivative of Bacillus subtilis 168 which was transformed by lpaB3gene. The result showed GEB3 only produced lip...MALDI-TOF-MS technology was used for identification of lipopeptide antibiotics producedby GEB3 strain, a derivative of Bacillus subtilis 168 which was transformed by lpaB3gene. The result showed GEB3 only produced lipopeptide antibiotic surfactin. The analysisby LC-MS demonstrated that GEB3 produced standard surfactin isoforms with side chainlengths of 13,14 and 15 carbon atoms. The bioactivity detection of surfactin indicatedthat the surfactin produced by GEB3 had inhibition effect on plant pathogens Rhizoctoniasolani and Pyricularia oryzae.展开更多
文摘For the first time, mass spectrometric (MS) techniques were employed to rapidly detect the pathogen Chalara fraxinea in-vitro and directly in-vivo in tissues of diseased ash trees caused by C. fraxinea, using a range of characteristic novel secondary metabolites of C. fraxinea as chemical markers for the presence of the pathogen. We have found an evident correlation between the presence and amount of these-only for C. fraxinea characteristic and novel-secondary metabolites (named chalarafraxinines) and the degree of disease of respective infected ash seedlings. As demonstrated in this work, the MS based high-throughput-screening approach constitute an alternative to the time consuming and expensive micro biological isolation procedures for detection of the pathogen C. fraxinea and furthermore, can be used to rapidly test ash genotypes for resistance / susceptibility to C. fraxinea infection.
基金supported by the National Nature1 Science Foundation of China(30170623)the National 863 Program of China(2001AA246013).
文摘MALDI-TOF-MS technology was used for identification of lipopeptide antibiotics producedby GEB3 strain, a derivative of Bacillus subtilis 168 which was transformed by lpaB3gene. The result showed GEB3 only produced lipopeptide antibiotic surfactin. The analysisby LC-MS demonstrated that GEB3 produced standard surfactin isoforms with side chainlengths of 13,14 and 15 carbon atoms. The bioactivity detection of surfactin indicatedthat the surfactin produced by GEB3 had inhibition effect on plant pathogens Rhizoctoniasolani and Pyricularia oryzae.
