Objective:To evaluate toxic activities of essential oils obtained from Rosmarinus officinalis and Lavandula stoechas against the fourth larval instars of Orgyia trigotephras.Methods:A total of 1 200 larvae were divide...Objective:To evaluate toxic activities of essential oils obtained from Rosmarinus officinalis and Lavandula stoechas against the fourth larval instars of Orgyia trigotephras.Methods:A total of 1 200 larvae were divided into three groups Ⅰ,Ⅱ,Ⅲ.Group Ⅰ was to investigate the effect of extracted essential oils from these aromatic plants as gastric disturbance.Bacillus thuringiensis and ethanol were used as control group.Group Ⅱ was used as contact action and Group Ⅲ was used as fumigant action.Oecis and ethanol were used as control group.During the three experiments,the effect of essential oils on larvae was assessed.Results:The chemical composition of essential oils from two medicinal plants was determined and,their insecticidal effects on the fourth larval slate of Orgyia trigotephras were assessed.The two simples presented an insecticidal activity,nevertheless Rosmarinus officinalis essential oil was less efficient compared to Lavandula stoechas one are discussed.Conclusions:The relationship between the chemical composition and the biologieal activities is confirmed by the present findings.Therefore the potential uses of these essential oils as bioinsecticides can he considered as an alternative to the use of synthetic products.展开更多
Objective Alzheimer＇s disease（AD） is one of the most important neurodegenerative disorders.It is characterized by dementia including def icits in learning and memory.The present study aimed to evaluate the effects ...Objective Alzheimer＇s disease（AD） is one of the most important neurodegenerative disorders.It is characterized by dementia including def icits in learning and memory.The present study aimed to evaluate the effects of aqueous extract of lavender（Lavandula angustifolia） on spatial performance of AD rats.Methods Male Wistar rats were f irst divided into control and AD groups.Rat model of AD was established by intracerebroventricular injection of 10 ？g A？1-42 20 d prior to administration of the lavender extract.Rats in both groups were then introduced to 2 stages of task learning（with an interval of 20 d） in Morris water maze,each followed by one probe test.After the f irst stage of spatial learning,control and AD animals received different doses（50,100 and 200 mg/kg） of the lavender extract.Results In the first stage of experiment,the latency to locate the hidden platform in AD group was significantly higher than that in control group.However,in the second stage of experiment,control and AD rats that received distilled water（vehicle） showed similar performance,indicating that the maze navigation itself could improve the spatial learning of AD animals.Besides,in the second stage of experiment,control and AD rats that received lavender extract administration at different doses（50,100,and 200 mg/kg） spent less time locating the platform（except for the AD rats with 50 mg/kg extract treatment）,as compared with their counterparts with vehicle treatment,respectively.In addition,lavender extract significantly improved the performance of control and AD rats in the probe test,only at the dose of 200 mg/kg,as compared with their counterparts with vehicle treatment.Conclusion The lavender extract can effectively reverse spatial learning def icits in AD rats.展开更多
Near-infrared and mid-infrared spectroscopies were currently used to analyze natural compounds. During the last ten years various multiblocks methods were developed such as Concatenated PLS, Hierarchical-PLS (H-PLS), ...Near-infrared and mid-infrared spectroscopies were currently used to analyze natural compounds. During the last ten years various multiblocks methods were developed such as Concatenated PLS, Hierarchical-PLS (H-PLS), and MultiBlock-PLS (MB-PLS). These three algorithms were used to analyze 55 lavender (Lavandula angustifolia) essential oil samples. The results obtained were compared to the ones obtained respectively in NIR and MIR ranges. The accuracies of the models depend on the spectroscopic technique, pretreatment and the PLS methods. The results showed that the choice of the factor numbers used to build the multiblock models was the most important parameter for the H-PLS and MB-PLS methods.展开更多
An important marker in neurodegenerative Alzheimer’s disease (AD) is abnormal production of amyloid beta (Aβ) peptide leading to formation of plaques in the brain. Through decreasing Aβ aggregates, anti-inflammator...An important marker in neurodegenerative Alzheimer’s disease (AD) is abnormal production of amyloid beta (Aβ) peptide leading to formation of plaques in the brain. Through decreasing Aβ aggregates, anti-inflammatory agents, phagocytosis, and proteolytic enzymes are known to decline risk of Aβ plaque formation. In the previous study we showed that aqueous extract of Lavandula angustifolia (lavender), with known anti-inflammatory effects, improves memory deficits in animal model of Alzheimer. Here, we assess if lavender play a role in clearance of Aβ plaques in the hippocampus. The Alzheimeric animals were created with intracerebroventricular injection of Aβ 1-42. To confirm formation of Aβ plaques, brain sections were stained by Congo red method. Twenty days post-injection they were administered with different doses (50, 100 and 200 mg/kg) of the aqueous extract of lavender for duration of 20 days. Our results demonstrated that 50 mg/kg of lavender not effectively influenced the Aβ plaques. On the other hand, the herbal medicine at the doses of 100 and 200 mg/kg markedly decreased the extent of Aβ aggregates. We concluded that the lavender extract dose dependently underlies elimination of Aβ plaques. The exact mechanism by which the herbal medicine removes the Aβ aggregates needs to be elucidated.展开更多
本研究采用高通量转录组测序技术,以生长在哈尔滨地区的薰衣草(Lavandula angustifolia Mill.)叶片为材料,对不同温度(26.67℃,F;32.07℃,S;-0.76℃,T)下获得的基因进行功能分类注释,分析了薰衣草转录组的整体表达特征及光合作用通路中...本研究采用高通量转录组测序技术,以生长在哈尔滨地区的薰衣草(Lavandula angustifolia Mill.)叶片为材料,对不同温度(26.67℃,F;32.07℃,S;-0.76℃,T)下获得的基因进行功能分类注释,分析了薰衣草转录组的整体表达特征及光合作用通路中差异表达基因响应温度变化的调控机制。结果表明,薰衣草叶片转录组共有90205条unigenes和315378条transcript,GC含量为42.12%,N50长度为1535 bp,表明转录组组装完整性较高。薰衣草unigenes在生物过程中涉及的功能亚类最多,其中,细胞过程和代谢过程所占比例最高达50.28%。在生物过程中,T vs S、F vs S、T vs F时均为光合作用富集程度最高。CCA分析表明差异基因的表达受温度的显著影响(P<0.01)。其中,T vs S时,下调表达基因比例高于上调表达基因,但在光合作用通路中,PSⅡ中的PsbD呈上调表达,可促进PSⅡ的修复;同时,PSⅠ中的PsaA呈上调表达,可保护PSI复合物,有助于植物在低温下维持正常光合作用。在光合天线蛋白通路中,F vs S时,上调表达基因比例高于下调表达基因,捕光复合体Ⅱ叶绿素a/b结合蛋白基因Lhcb3、Lhcb4、Lhcb5上调表达,利于植物维持PSII-LHCⅡ超复合体的稳定性,发挥光保护作用。本研究结果可为薰衣草的分子遗传育种及在中国东北地区种植推广奠定理论基础。展开更多
Objective:To evaluate toxic activities of essential oils obtained from Rosmarinus officinalis and Lavandula stoechas against the fourth larval instars of Orgyia trigotephras.Methods:A total of 1200 larvae were divided...Objective:To evaluate toxic activities of essential oils obtained from Rosmarinus officinalis and Lavandula stoechas against the fourth larval instars of Orgyia trigotephras.Methods:A total of 1200 larvae were divided into three groups-I,II,III.Group I was to investigate the effect of extracted essential oils from these aromatic plants as gastric disturbance.Bacillus thuringiensis was used as referencee and ethanol as control.Group II was used as contact action and Group III was used as fumigant action.For both Groups II and III,Decis was used as reference and ethanol as control.During the three experiments,the effect of essential oils on larvae was assessed.Results:The chemical composition of essential oils from two medicinal plants was determined,and their insecticidal effects on the fourth larval state of Orgyia trigotephras were assessed.They presented an insecticidal activity.Rosmarinus officinalis essential oil was less efficient compared to Lavandula stoechas.Conclusions:The relationship between the chemical composition and the biological activities is confirmed by the present findings.Therefore the potential uses of these essential oils as bioinsecticides can be considered as an alternative to the use of synthetic products.展开更多
文摘Objective:To evaluate toxic activities of essential oils obtained from Rosmarinus officinalis and Lavandula stoechas against the fourth larval instars of Orgyia trigotephras.Methods:A total of 1 200 larvae were divided into three groups Ⅰ,Ⅱ,Ⅲ.Group Ⅰ was to investigate the effect of extracted essential oils from these aromatic plants as gastric disturbance.Bacillus thuringiensis and ethanol were used as control group.Group Ⅱ was used as contact action and Group Ⅲ was used as fumigant action.Oecis and ethanol were used as control group.During the three experiments,the effect of essential oils on larvae was assessed.Results:The chemical composition of essential oils from two medicinal plants was determined and,their insecticidal effects on the fourth larval slate of Orgyia trigotephras were assessed.The two simples presented an insecticidal activity,nevertheless Rosmarinus officinalis essential oil was less efficient compared to Lavandula stoechas one are discussed.Conclusions:The relationship between the chemical composition and the biologieal activities is confirmed by the present findings.Therefore the potential uses of these essential oils as bioinsecticides can he considered as an alternative to the use of synthetic products.
基金supported by Deputy of Research,Kashan University of Medical Sciences,Kashan,I.R. Iran (No. 8835)
文摘Objective Alzheimer＇s disease（AD） is one of the most important neurodegenerative disorders.It is characterized by dementia including def icits in learning and memory.The present study aimed to evaluate the effects of aqueous extract of lavender（Lavandula angustifolia） on spatial performance of AD rats.Methods Male Wistar rats were f irst divided into control and AD groups.Rat model of AD was established by intracerebroventricular injection of 10 ？g A？1-42 20 d prior to administration of the lavender extract.Rats in both groups were then introduced to 2 stages of task learning（with an interval of 20 d） in Morris water maze,each followed by one probe test.After the f irst stage of spatial learning,control and AD animals received different doses（50,100 and 200 mg/kg） of the lavender extract.Results In the first stage of experiment,the latency to locate the hidden platform in AD group was significantly higher than that in control group.However,in the second stage of experiment,control and AD rats that received distilled water（vehicle） showed similar performance,indicating that the maze navigation itself could improve the spatial learning of AD animals.Besides,in the second stage of experiment,control and AD rats that received lavender extract administration at different doses（50,100,and 200 mg/kg） spent less time locating the platform（except for the AD rats with 50 mg/kg extract treatment）,as compared with their counterparts with vehicle treatment,respectively.In addition,lavender extract significantly improved the performance of control and AD rats in the probe test,only at the dose of 200 mg/kg,as compared with their counterparts with vehicle treatment.Conclusion The lavender extract can effectively reverse spatial learning def icits in AD rats.
文摘Near-infrared and mid-infrared spectroscopies were currently used to analyze natural compounds. During the last ten years various multiblocks methods were developed such as Concatenated PLS, Hierarchical-PLS (H-PLS), and MultiBlock-PLS (MB-PLS). These three algorithms were used to analyze 55 lavender (Lavandula angustifolia) essential oil samples. The results obtained were compared to the ones obtained respectively in NIR and MIR ranges. The accuracies of the models depend on the spectroscopic technique, pretreatment and the PLS methods. The results showed that the choice of the factor numbers used to build the multiblock models was the most important parameter for the H-PLS and MB-PLS methods.
文摘An important marker in neurodegenerative Alzheimer’s disease (AD) is abnormal production of amyloid beta (Aβ) peptide leading to formation of plaques in the brain. Through decreasing Aβ aggregates, anti-inflammatory agents, phagocytosis, and proteolytic enzymes are known to decline risk of Aβ plaque formation. In the previous study we showed that aqueous extract of Lavandula angustifolia (lavender), with known anti-inflammatory effects, improves memory deficits in animal model of Alzheimer. Here, we assess if lavender play a role in clearance of Aβ plaques in the hippocampus. The Alzheimeric animals were created with intracerebroventricular injection of Aβ 1-42. To confirm formation of Aβ plaques, brain sections were stained by Congo red method. Twenty days post-injection they were administered with different doses (50, 100 and 200 mg/kg) of the aqueous extract of lavender for duration of 20 days. Our results demonstrated that 50 mg/kg of lavender not effectively influenced the Aβ plaques. On the other hand, the herbal medicine at the doses of 100 and 200 mg/kg markedly decreased the extent of Aβ aggregates. We concluded that the lavender extract dose dependently underlies elimination of Aβ plaques. The exact mechanism by which the herbal medicine removes the Aβ aggregates needs to be elucidated.
文摘本研究采用高通量转录组测序技术,以生长在哈尔滨地区的薰衣草(Lavandula angustifolia Mill.)叶片为材料,对不同温度(26.67℃,F;32.07℃,S;-0.76℃,T)下获得的基因进行功能分类注释,分析了薰衣草转录组的整体表达特征及光合作用通路中差异表达基因响应温度变化的调控机制。结果表明,薰衣草叶片转录组共有90205条unigenes和315378条transcript,GC含量为42.12%,N50长度为1535 bp,表明转录组组装完整性较高。薰衣草unigenes在生物过程中涉及的功能亚类最多,其中,细胞过程和代谢过程所占比例最高达50.28%。在生物过程中,T vs S、F vs S、T vs F时均为光合作用富集程度最高。CCA分析表明差异基因的表达受温度的显著影响(P<0.01)。其中,T vs S时,下调表达基因比例高于上调表达基因,但在光合作用通路中,PSⅡ中的PsbD呈上调表达,可促进PSⅡ的修复;同时,PSⅠ中的PsaA呈上调表达,可保护PSI复合物,有助于植物在低温下维持正常光合作用。在光合天线蛋白通路中,F vs S时,上调表达基因比例高于下调表达基因,捕光复合体Ⅱ叶绿素a/b结合蛋白基因Lhcb3、Lhcb4、Lhcb5上调表达,利于植物维持PSII-LHCⅡ超复合体的稳定性,发挥光保护作用。本研究结果可为薰衣草的分子遗传育种及在中国东北地区种植推广奠定理论基础。
文摘Objective:To evaluate toxic activities of essential oils obtained from Rosmarinus officinalis and Lavandula stoechas against the fourth larval instars of Orgyia trigotephras.Methods:A total of 1200 larvae were divided into three groups-I,II,III.Group I was to investigate the effect of extracted essential oils from these aromatic plants as gastric disturbance.Bacillus thuringiensis was used as referencee and ethanol as control.Group II was used as contact action and Group III was used as fumigant action.For both Groups II and III,Decis was used as reference and ethanol as control.During the three experiments,the effect of essential oils on larvae was assessed.Results:The chemical composition of essential oils from two medicinal plants was determined,and their insecticidal effects on the fourth larval state of Orgyia trigotephras were assessed.They presented an insecticidal activity.Rosmarinus officinalis essential oil was less efficient compared to Lavandula stoechas.Conclusions:The relationship between the chemical composition and the biological activities is confirmed by the present findings.Therefore the potential uses of these essential oils as bioinsecticides can be considered as an alternative to the use of synthetic products.
