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淫羊藿苷调控LAP自噬促进酒精抑制的MC3T3-E1细胞成骨分化 被引量:2
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作者 曾麒 陈跃平 +2 位作者 宋世雷 赖渝 吴华华 《中国中药杂志》 北大核心 2025年第3期590-599,共10页
研究细胞自噬在MC3T3-E1细胞成骨诱导(生理)、酒精(alcohol,AL)干预(病理)分化过程中的影响机制,以及淫羊藿苷(icariin,ICA)对AL干预病理状态下MC3T3-E1细胞成骨分化的作用机制。成骨矿化结节染色鉴定该细胞可分化为成骨细胞。CCK-8实... 研究细胞自噬在MC3T3-E1细胞成骨诱导(生理)、酒精(alcohol,AL)干预(病理)分化过程中的影响机制,以及淫羊藿苷(icariin,ICA)对AL干预病理状态下MC3T3-E1细胞成骨分化的作用机制。成骨矿化结节染色鉴定该细胞可分化为成骨细胞。CCK-8实验筛选合适的AL和ICA浓度后,实验分为7组:完全培养基(complete medium,CM)组,成骨诱导培养基(osteogenic induction medium,OIM)组,OIM+0.25 mol·L^(-1)AL组,OIM+0.25 mol·L^(-1)AL+1×10^(-8)mol·L^(-1)ICA组,OIM+0.25 mol·L^(-1)AL+1×10^(-7)mol·L^(-1)ICA组,OIM+0.25 mol·L^(-1)AL+1×10^(-6)mol·L^(-1)ICA组,OIM+0.25 mol·L^(-1)AL+1×10^(-5)mol·L^(-1)ICA组,培养7 d。碱性磷酸酶(alkaline phosphatase,ALP)染色检测ALP相对面积,Western blot、RT-qPCR检测成骨、自噬相关蛋白及mRNA表达情况,活性氧(reactive oxygen species,ROS)染色检测ROS水平,细胞凋亡线粒体膜电位实验检测细胞凋亡情况。结果显示,ICA可上调AL干预后下调的ALP相对面积;AL下调成骨相关蛋白Wnt家族成员1(Wnt family member 1 gene,Wnt1)及成骨相关基因Wnt1、β-连环蛋白(β-catenin)、核心结合因子2(Runt-related transcription factor 2,Runx2)、骨保护素(osteoprotegerin,OPG)、ALP的表达水平,抑制细胞成骨分化,ICA可上调AL抑制的成骨相关蛋白及mRNA的表达水平,促进细胞成骨分化;AL抑制典型自噬,ICA调控Rubicon抑制LC3相关吞噬作用(LC3-associated phagocytosis,LAP)促进典型自噬;ICA下调AL诱导后上升的ROS水平;ICA下调AL干预后导致的成骨细胞凋亡。综上,ICA可调控Rubicon抑制LAP促进典型自噬,清除ROS减少细胞凋亡,最终通过调控Wnt/β-catenin信号通路促进AL干预病理状态下MC3T3-E1细胞的成骨分化。 展开更多
关键词 淫羊藿苷 酒精 成骨细胞 MC3T3-E1细胞 Wnt1/β-catenin通路 成骨分化 骨代谢失衡 自噬 lap
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Achievement of superb-strength lap joint via opposite-directions flowing friction stir lap welding of 2024 aluminum alloys
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作者 Zhiqing ZHANG Peng GONG +4 位作者 Shude JI Lin MA Chen JIN Xiuyan LI Qi SONG 《Chinese Journal of Aeronautics》 2025年第8期588-603,共16页
It is common for the rotating pin largely plunging into lower sheet to break up lap interface of Friction Stir Lap Welding(FSLW)joint,but the unavoidable up-bending morphology of hook outside Nugget Zone(NZ)largely re... It is common for the rotating pin largely plunging into lower sheet to break up lap interface of Friction Stir Lap Welding(FSLW)joint,but the unavoidable up-bending morphology of hook outside Nugget Zone(NZ)largely reduces the joint bearing ability.Based on the novel Opposite-directions Flowing FSLW(OF-FSLW)by the self-developed rotating tool with an Xshaped right-left thread pin,the 2024 aluminum alloys lap joint was successfully welded in this study.The migration law of lap interface during welding was investigated by the experimental and numerical methods,and then how the rotating pin and its rotating velocity affect the formation and strength of OF-FSLW joint was further analyzed.The results show that the Material Concentrated Zone(MCZ)which formed above the original lap interface made the hook bend downward,the NZ greatly enlarged and the beginning part of cold lap compressed and thickened,thereby heightening the joint bearing ability.For the OF-FSLW joint,its maximum tensile strength was403 MPa,and the corresponding joint efficiency of 90.8%was an incredible and superb value for the 2000 series heat-treatment strengthened aluminum alloys friction stir welded joint.The OF-FSLW technology by the rotating tool with an X-shaped right-left thread pin is proven to be a greatly effective approach for manufacturing the aluminum alloys lap joint with superb strength. 展开更多
关键词 2024-T4 aluminum alloys Opposite-directions flowing friction stir lapwelding Rotatingtool withanXshaped right-left thread pin lap interface Tensile strength
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Dissimilar Friction Stir Lap Welding of Ti Alloy and Al-Li Alloy:Microstructure and Mechanical Property 被引量:1
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作者 Zhang Wenxin Zhang Xiankun +3 位作者 Shi Lei Li Shengli Jiang Yuanning Wu Chuansong 《稀有金属材料与工程》 北大核心 2025年第2期311-318,共8页
Friction stir lap welding of AA2195 Al-Li alloy and Ti alloy was conducted to investigate the formation,microstructure,and mechanical properties of the joints.Results show that under different welding parameters,with ... Friction stir lap welding of AA2195 Al-Li alloy and Ti alloy was conducted to investigate the formation,microstructure,and mechanical properties of the joints.Results show that under different welding parameters,with the decrease in welding heat input,the weld surface is smoother.The Ti/Al joint interface is flat without obvious Ti and Al mixed structure,and the hook structure is not formed under optimal parameters.Due to the enhanced breaking effect of the stirring head,the hook structural defects and intermetallic compounds are more likely to form at the Ti/Al interface at high rotational speed of 1000 r/min,thereby deteriorating the mechanical properties of joints.Decreasing the heat input is beneficial to hardness enhancement of the aluminum alloy in the weld nugget zone.Under the optimal parameters of rotation speed of 800 r/min and welding speed of 120 mm/min,the maximum tensile shear strength of joint is 289 N/mm. 展开更多
关键词 Ti/Al dissimilar welding friction stir lap welding Ti alloy Al-Li alloy interface bonding mechanical property
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Elucidating the process mechanism in Mg-to-Al friction stir lap welding enhanced by ultrasonic vibration 被引量:3
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作者 Ming Zhai Lei Shi ChuanSong Wu 《Journal of Magnesium and Alloys》 2025年第1期338-355,共18页
The composite structures/components made by friction stir lap welding(FSLW)of Mg alloy sheet and Al alloy sheet are of wide application potentials in the manufacturing sector of transportation vehicles.To further impr... The composite structures/components made by friction stir lap welding(FSLW)of Mg alloy sheet and Al alloy sheet are of wide application potentials in the manufacturing sector of transportation vehicles.To further improve the joint quality,the ultrasonic vibration(UV)is exerted in FSLW,and the UV enhanced FSLW(UVeFSLW)was developed for making Mg-to-Al dissimilar joints.The numerical analysis and experimental investigation were combined to study the process mechanism in Mg/Al UVeFSLW.An equation related to the temperature and strain rate was derived to calculate the grain size at different locations of the weld nugget zone,and the effect of grain size distribution on the threshold thermal stress was included,so that the prediction accuracy of flow stress was further improved.With such modified constitutive equation,the numerical simulation was conducted to compare the heat generation,temperature profiles and material flow behaviors in Mg/Al UVeFSLW/FSLW processes.It was found that the exerted UV decreased the temperature at two checking points on the tool/workpiece interface from 707/671 K in FSLW to 689/660 K in UVeFSLW,which suppressed the IMCs thickness at Mg-Al interface from 1.7μm in FSLW to 1.1μm in UVeFSLW.The exerted UV increased the horizontal materials flow ability,and decreased the upward flow ability,which resulted in the increase of effective sheet thickness/effective lap width from 2.01/3.70 mm in FSLW to 2.04/4.84 mm in UVeFSLW.Therefore,the ultrasonic vibration improved the tensile shear strength of Mg-to-Al lap joints by 18%. 展开更多
关键词 Friction stir lap welding Mg-to-Al dissimilar alloys Ultrasonic vibration Numerical simulation Experimental investigation
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Theoretical and Experimental Investigations on Thickness Uniformity in Double-sided Lapping
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作者 Zhuolin Cai Zhe Yang +4 位作者 Bo Pan Jiale Lian Lianlin Wang Sergei Pronkevich Jiang Guo 《Chinese Journal of Mechanical Engineering》 2025年第3期151-163,共13页
The double-sided lapping process is extensively employed in the manufacturing of wafers,optical windows,and seal rings due to its high efficiency and ability to achieve precise flatness.However,limited research has ex... The double-sided lapping process is extensively employed in the manufacturing of wafers,optical windows,and seal rings due to its high efficiency and ability to achieve precise flatness.However,limited research has explored the thickness uniformity among different workpieces after double-sided lapping,and the underlying mechanism remains unclear.To address the demand for higher precision,this paper first analyzed the relative kinematic model between the workpiece and the lapping plate to clarify the causes of thickness variations among workpieces after double-sided lapping.Subsequently,a finite element method(FEM)model was developed to account for the pressure distribution on the workpiece surfaces at the initial stage of the process.The results indicate that the number of workpieces influences the final thickness variation.Then,various sets of thin copper plates with different thicknesses were lapped,and the findings revealed that five copper plates processed simultaneously exhibited more uniform thickness compared to the three plates.The experimental results align well with the theoretical analysis.Ultimately,a thickness variation of less than 6μm was achieved on five copper plates measuringΦ100×2.9 mm.This study presents a comprehensive analysis of the mechanisms influencing thickness uniformity in the double-sided lapping process and provides practical guidelines for optimizing the process to achieve stringent precision standards in industrial applications. 展开更多
关键词 Double-sided lapping Thickness uniformity Theoretical analysis FEM Thin copper plate
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Hole inhibition mechanisms of Mg/steel lap joint by pinless friction stir spot welding
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作者 Tao-tao Li Hong-de Ding +5 位作者 Rui-feng Li Kai Qi Zhen-guang Liu Xiao-qiang Zhang Yue Zhao Lei Qiao 《Journal of Iron and Steel Research International》 2025年第4期1085-1101,共17页
Pinless friction stir spot welding(P-FSSW)was performed to manufacture Mg/steel lap joints.Orthogonal tests for P-FSSW of Mg/steel were investigated,and the main factors affecting the properties of Mg/steel lap joints... Pinless friction stir spot welding(P-FSSW)was performed to manufacture Mg/steel lap joints.Orthogonal tests for P-FSSW of Mg/steel were investigated,and the main factors affecting the properties of Mg/steel lap joints were derived.The shear force of the Mg/steel lap joints gradually increased and then decreased as the welding time increased.Maximum shear force was 5.3 kN.Fe-Al intermetallic compound(IMC)was formed at the Mg/steel interface near the steel side,and Mg-Al IMCs were formed at the Mg/steel interface near the Mg alloy side.Mg/steel lap joint was transformed from an initial solid-state welding to fusion-brazing welding as the welding time increased.No hole defects were formed in Mg/steel solid-state welding joints,whereas hole defects appeared in Mg/steel fusion-brazing welding joints.The temperature field of Mg/steel lap joints was simulated to analyze hole defects generated during the welding process.Hole defects can be eliminated by changing the spindle deflection angle,and the shear force decreased.Excessive spindle deflection can also lead to failure to form a stable joint.Hole defects were removed because the spindle deflection angle reduced the interfacial reaction temperature,and a solid-state welding joint was formed,which resulted in an absence of fusion-brazing welding hole formation. 展开更多
关键词 Mg/steel lap joint Pinless friction stir spot welding Hole inhibition SIMULATION
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Improving microstructure,tensile properties and corrosion resistance of AA6016 and AA2519 alloys friction stir lap joints via TIG arc
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作者 Tie YI Sheng-dan LIU +2 位作者 Chen FANG Geng-duo JIANG Xuan-xuan DAI 《Transactions of Nonferrous Metals Society of China》 2025年第2期405-417,共13页
The effects of tungsten inert gas arc-assisted friction stir welding(TIG-FSW)on the microstructure,tensile properties and corrosion resistance of AA6016 and AA2519 alloys lap joints were investigated by means of optic... The effects of tungsten inert gas arc-assisted friction stir welding(TIG-FSW)on the microstructure,tensile properties and corrosion resistance of AA6016 and AA2519 alloys lap joints were investigated by means of optical microscope,scanning electron microscope,tensile test at room temperature,corrosion immersion tests and electrochemical measurements.The results show that the introduction of TIG arc during FSW process results in a more uniform microstructure of the joint with no tunnel hole defects.Furthermore,it enhances tensile strength and elongation of the joint with increased rates of 11.5%and 50.0%,respectively;meanwhile,the corrosion current density and largest corrosion depth are decreased with reduction rates of 78.2%and 45.7%,respectively.TIG-FSW can promote flow,contact and diffusion of materials,thus improving microstructure of the joint.Additionally,it reduces the size and number of secondary phase particles.Consequently,these factors contribute to the higher tensile properties and corrosion resistance of the joints. 展开更多
关键词 friction stir welding lap joint dissimilar aluminum alloys secondary phase particles MICROSTRUCTURE tensile properties corrosion resistance
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LAP3经JAK2/STAT3途径促进胃癌MGC-803细胞的增殖
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作者 伍石华 全婧丹 +2 位作者 王化修 吕小元 张志伟 《湘南学院学报(医学版)》 2025年第3期1-6,共6页
目的探讨胃癌相关蛋白质LAP3对胃癌细胞增殖的影响及其分子机制。方法采用慢病毒感染胃癌MGC-803细胞,筛选后建立LAP3稳定低表达MGC-803细胞系,分为对照组(MGC-803-shCtrl)、干扰组(MGC-803-shLAP3-1和MGC-803-shLAP3-2),实时荧光定量PC... 目的探讨胃癌相关蛋白质LAP3对胃癌细胞增殖的影响及其分子机制。方法采用慢病毒感染胃癌MGC-803细胞,筛选后建立LAP3稳定低表达MGC-803细胞系,分为对照组(MGC-803-shCtrl)、干扰组(MGC-803-shLAP3-1和MGC-803-shLAP3-2),实时荧光定量PCR和Western Blot检测LAP3表达情况;通过CCK-8法和平板克隆形成实验检测LAP3低表达对胃癌MGC-803细胞增殖的影响;采用Western Blot检测LAP3低表达对胃癌MGC-803细胞中JAK2/STAT3途径的影响;构建裸鼠皮下荷瘤模型,观察LAP3低表达对胃癌MGC-803细胞体内成瘤能力的影响,并通过免疫组化染色检测瘤组织中Ki-67的表达情况。结果MGC-803-shLAP3-1和MGC-803-shLAP3-2组细胞增殖率较MGC-803-shCtrl组均下降,细胞集落数减少(均P<0.01)。MGC-803-shLAP3-1和MGC-803-shLAP3-2组JAK2和STAT3蛋白的磷酸化水平较MGC-803-shCtrl组均降低(均P<0.001)。裸鼠成瘤结果显示,MGC-803-shLAP3-1和MGC-803-shLAP3-2组的肿瘤体积与重量较MGC-803-shCtrl组均下降(均P<0.001);MGC-803-shLAP3-1和MGC-803-shLAP3-2组的肿瘤组织Ki-67阳性表达率较MGC-803-shCtrl组均降低(均P<0.001)。结论LAP3可能通过活化JAK2/STAT3途径促进胃癌细胞的增殖。 展开更多
关键词 胃癌 lap3 JAK2/STAT3途径 细胞增殖
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莱斯特评估包(LAP)结合PDCA循环质控指导全科规培住院医师门诊接诊能力的教学实践研究
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作者 尹向辉 孙军奎 +7 位作者 贺连栋 刘宝明 韩芳 李索南措 薛兴翠 汪丽玲 郭蕊 白小林 《青海医药杂志》 2024年第8期60-63,共4页
目的:探讨莱斯特评估包(LAP)结合PDCA循环质控指导全科规范化培训住院医师(简称全科住培医师)在门诊接诊能力的教学实践效果。方法:收集2023年1月—2023年12月于青海红十字医院及西宁市城北区马坊社区、城东区东关社区参与全科住培医师... 目的:探讨莱斯特评估包(LAP)结合PDCA循环质控指导全科规范化培训住院医师(简称全科住培医师)在门诊接诊能力的教学实践效果。方法:收集2023年1月—2023年12月于青海红十字医院及西宁市城北区马坊社区、城东区东关社区参与全科住培医师培训的2021级及2022级医师40名,随机分为对照组和观察组各20名。对照组接受LAP的教学模式,观察组接受LAP联合PDCA循环质控指导的教学模式。每周进行考核,4周考核结束后进行匿名填写满意度调查表。结果:通过定期的门诊培训及考核,培训前及培训第二周,两组全科住培医师LAP总分比较,差异均无统计学意义(P>0.05),培训第一周、第三周、第四周,观察组全科住培医师LAP总分分别为(75.58±2.48)分、(82.13±2.17)分、(86.80±2.18)分,均高于对照组(P<0.05)。在带教第四周后,观察组全科住培医师首诊病史采集评分为(17.10±0.84)分、解决问题评分为(17.88±1.11)分,均高于对照组(P <0.05),两组全科住培医师体格检查评分、患者管理评分、医患关系评分、预防保健评分及病历记录评分比较,差异均无统计学意义(P>0.05)。教学满意度调查中观察组非常满意16名(80.0%),对照组3名(15.0%),两组比较,差异有统计学意义(P <0.05)。结论:LAP结合PDCA循环质控指导应用于全科住培医师门诊接诊能力临床教学中有较好的效果,值得临床推广应用。 展开更多
关键词 lap PDCA 全科 规培住院医师门诊教学
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Strengthening strategy for high-performance friction stir lap welded joints based on 5083 Al alloy 被引量:1
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作者 Yujia Shen Jijie Wang +5 位作者 Beibei Wang Peng Xue Fengchao Liu Dingrui Ni Bolv Xiao Zongyi Ma 《International Journal of Minerals,Metallurgy and Materials》 SCIE EI CAS CSCD 2024年第11期2498-2507,共10页
During aircraft,ship,and automobile manufacturing,lap structures are frequently produced among Al alloy skins,wall panels,and stiffeners.The occurrence of welding defects severely decreases mechanical properties durin... During aircraft,ship,and automobile manufacturing,lap structures are frequently produced among Al alloy skins,wall panels,and stiffeners.The occurrence of welding defects severely decreases mechanical properties during friction stir lap welding(FSLW).This study focuses on investigating the effects of rotation rate,multipass welding,and cooling methods on lap defect formation,microstructural evolution,and mechanical properties.Hook defects were eliminated by decreasing welding speed,applying two-pass FLSW with a small welding tool,and introducing additional water cooling,thus leading to a remarkable increase in effective sheet thickness and lap width.This above strategy yielded defect-free joints with an ultrafine-grained microstructure and increased tensile shear force from 298 to 551 N/mm.The fracture behavior of FSLW joints was systematically studied,and a fracture factor of lap joints was proposed to predict their fracture mode.By reducing the rotation rate,using two-pass welding,and employing additional water cooling strategies,an enlarged,strengthened,and defect-free lap zone with refined ultrafine grains was achieved with a quality comparable to that of lap welds based on 7xxx Al alloys.Importantly,this study provides a valuable FSLW method for eliminating hook defects and improving joint performance. 展开更多
关键词 friction stir lap welding defect control microstructure fracture mechanisms mechanical properties
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Bulging Distortion of Austenitic Stainless Steel Sheet on the Partially Penetrated Side of Non-Penetration Lap Laser Welding Joint
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作者 Chengwu Yao Enze Liu +1 位作者 Jiaming Ni Binying Nie 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2024年第1期286-295,共10页
Non-penetration laser welding of lap joints in austenitic stainless steel sheets is commonly preferred in fields where the surface quality is of utmost importance.However,the application of non-penetration welded aust... Non-penetration laser welding of lap joints in austenitic stainless steel sheets is commonly preferred in fields where the surface quality is of utmost importance.However,the application of non-penetration welded austenitic stainless steel parts is limited owing to the micro bulging distortion that occurs on the back surface of the partial penetration side.In this paper,non-penetration lap laser welding experiments,were conducted on galvanized and SUS304 austenitic stainless steel plates using a fiber laser,to investigate the mechanism of bulging distortion.A comparative experiment of DC01 galvanized steel-Q235 carbon steel lap laser welding was carried out,and the deflection and distortion profile of partially penetrated side of the sheets were measured using a noncontact laser interferometer.In addition,the cold-rolled SUS304 was subjected to heat holding at different temperatures and water quenching after bending to characterize its microstructure under tensile and compressive stress.The results show that,during the heating stage of the thermal cycle of laser lap welding,the partial penetration side of the SUS304 steel sheet generates compressive stress,which extrudes the material in the heat-affected zone to the outside of the back of the SUS304 steel sheet,thereby forming a bulge.The findings of these experiments can be of great value for controlling the distortion of the partial penetrated side of austenitic stainless steel sheet during laser non-penetration lap welding. 展开更多
关键词 Non-penetration lap laser welding Bulging distortion Austenitic stainless steel Compressive stress Tension stress
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Photo-responsive Carboxymethyl Chitosan/Laponite Hydrogel as a Potential Spinal Cord Injury Scaffold:Characterization and Cytocompatibility Study
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作者 Jayanti Parajuli LI Yongtao +3 位作者 CHANG Likun YE Liyuan HAN Yingchao YIN Yixia 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS CSCD 2024年第6期1628-1636,共9页
We synthesized photo-responsive carboxymethyl chitosan(CMC-MA)via free radical polymerization and utilized nanoclay laponite(LAP)as an inorganic crosslinking agent to develop an injectable and 3D-printable CMC-MA/LAP ... We synthesized photo-responsive carboxymethyl chitosan(CMC-MA)via free radical polymerization and utilized nanoclay laponite(LAP)as an inorganic crosslinking agent to develop an injectable and 3D-printable CMC-MA/LAP hydrogel.We determined the optimal ratio of 2.5 w/v%CMC-MA/7.5 w/v%LAP based on injection molding,compression modulus,swelling properties,rheological properties,and 3D printing properties of the hydrogel system.In-vitro cytocompatibility experiments showed that both CMC-MA and CMC-MA/LAP hydrogel had no inhibitory effect on cell proliferation and can promote cell growth when cultured on the surface of the hydrogel matrix.Moreover,the hydrogel containing LAP particles significantly facilitated cell adhesion(>60%)compared with the hydrogel without LAP(20%).Our findings demonstrate that the CMC-MA/LAP hydrogel has great potential for tissue repair in neural tissue engineering. 展开更多
关键词 neural injectable hydrogels spinal cord injury CMC-MA/lap hydrogel CYTOCOMPATIBILITY
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A novel seal-flow multi-vortex friction stir lap welding of metal to polymer matrix composites
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作者 Shuaiqiang NIAN Mingshen LI +3 位作者 Shude JI Wei HU Zhiqing ZHANG Zelin SUN 《Chinese Journal of Aeronautics》 SCIE EI CAS CSCD 2024年第1期451-462,共12页
The friction stir lap welding(FSLW)of metal to polymer is a challenging work due to the unavoidable polymer overflowing.Facing this problem,a novel seal-flow multi-vortex friction stir lap welding(SM-FSLW)technology b... The friction stir lap welding(FSLW)of metal to polymer is a challenging work due to the unavoidable polymer overflowing.Facing this problem,a novel seal-flow multi-vortex friction stir lap welding(SM-FSLW)technology based on the subversively-designed multi-step pin was put forward.Choosing 7075 aluminum alloy and short glass fiber-reinforced polyether ether ketone(PEEK)as research subjects,the welding temperature,material flow,formation and tensile shear strength of dissimilar materials lap joint under the SM-FSLW were studied and compared with those under traditional FSLW based on the conical pin.The multi-step pin rather than the conical pin effectively hindered the polymer overflowing due to the formation of vortexes by the step,thereby attaining a joint with a smooth surface.Compared with traditional FSLW,the SMFSLW obtained the higher welding temperature,the more violent material flow and the larger area with high flow velocity,thereby producing the macro-mechanical and micro-mechanical interlockings and then heightening the joint loading capacity.The tensile shear strength of lap joint under SM-FSLW was 27.8% higher than that under traditional FSLW.The SM-FSLW technology using the multi-step pin provides an effective way on obtaining a heterogeneous lap joint of metal to polymer with the excellent formation and high strength. 展开更多
关键词 Friction stir lap welding Metal to polymer Multi-step pin Material flow Tensile shear strength
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RPE细胞光损伤相关新基因s-lap编码区克隆及s-lap/GFP融合蛋白在B16黑色素瘤细胞中的表达与定位 被引量:1
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作者 宋忆淑 李玉新 +2 位作者 费向东 鲍永利 谭大鹏 《眼科新进展》 CAS 2005年第1期11-13,共3页
目的 克隆新基因s lap编码区序列 ,研究其编码的蛋白质在B16黑色素瘤细胞内的表达与定位。方法 分析s lapcDNA序列 ,建立视网膜色素上皮 (retinalpigmentep ithelium ,RPE)细胞光损伤模型 ,RT PCR克隆其编码区序列 ,构建了带有绿色荧... 目的 克隆新基因s lap编码区序列 ,研究其编码的蛋白质在B16黑色素瘤细胞内的表达与定位。方法 分析s lapcDNA序列 ,建立视网膜色素上皮 (retinalpigmentep ithelium ,RPE)细胞光损伤模型 ,RT PCR克隆其编码区序列 ,构建了带有绿色荧光蛋白的目的基因真核表达重组质粒 pcDNA3 .1 GFP/s lap ,转染B16黑色素瘤细胞 ,观察s lap/GFP融合蛋白在B16黑色素瘤细胞内的表达与定位。结果 s lapcDNA序列含有编码10 1个氨基酸的开放读码框架 ,有 2个可能的N 糖基化位点 ,1个可能的caseinkinaseII磷酸化位点和 2个可能的PKC磷酸化位点 ;成功地克隆了s lap蛋白编码区序列 ,构建了其真核表达载体 ,荧光显微镜观察 ,在转染 pcDNA3 .1 GFP质粒的B16黑色素瘤细胞中 ,荧光呈网状分布于细胞浆内 ,而细胞核内低表达 ;在转染s lap/GFP融合基因的B16黑色素瘤细胞中 ,荧光均匀分布于整个细胞 ,尤其以细胞核内高表达。结论 新基因s lap编码的蛋白质可在B16黑色素瘤细胞中获得表达 ,并以细胞核内高表达。 展开更多
关键词 s-lap基因 克隆 s-lap/GFP 融合蛋白 基因表达 基因定位
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无机-有机杂化非线性光学晶体材料LAP和d-LAP晶体红外吸收边计算
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作者 莽朝永 吴克琛 +4 位作者 林晨升 刘萍 周张锋 洒荣建 庄伯涛 《光谱学与光谱分析》 SCIE EI CAS CSCD 北大核心 2003年第6期1076-1078,共3页
根据双原子谐振子模型近似,提出了估算分子晶体材料红外吸收边的理论方法,然后利用超分子量子化学从头算,计算了L-精氨酸一水磷酸盐(LAP)晶体和氘化L-精氨酸一水磷酸盐(d-LAP)晶体单元超分子的红外振动光谱,其中LAP晶体超分子计算值与... 根据双原子谐振子模型近似,提出了估算分子晶体材料红外吸收边的理论方法,然后利用超分子量子化学从头算,计算了L-精氨酸一水磷酸盐(LAP)晶体和氘化L-精氨酸一水磷酸盐(d-LAP)晶体单元超分子的红外振动光谱,其中LAP晶体超分子计算值与晶体红外光谱实验值吻合,表明超分子计算能有效地模拟无机-有机杂化非线性光学晶体的红外振动光谱。在归属了超分子重要基频线后,分析其红外强度,并估算了泛频频率。根据我们提出的方法,估算了这两种晶体的红外吸收边,结果与实验值较吻合。表明我们建议的理论方法是合理的。此外,通过计算表明,如果非线性光学晶体材料的红外吸收边是由与活泼H有关的伸缩振动泛频频率决定,那么活泼H的氘化是一种改善红外吸收边的有效途径。 展开更多
关键词 无机-有机杂化非线性光学晶体 L-精氨酸-水磷酸盐晶体 氘化L-精氨酸-水磷酸盐晶体 lap d-lap 红外吸收边 红外振动光谱 超分子计算
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基于卫星资料同化和LAPS-WRF模式系统的云天太阳辐射数值模拟改进方法 被引量:18
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作者 程兴宏 刘瑞霞 +4 位作者 申彦波 朱蓉 彭继达 杨振斌 徐洪雄 《大气科学》 CSCD 北大核心 2014年第3期577-589,共13页
太阳能光伏发电已成为仅次于水电和风能的第三大可再生能源,光伏发电受云量时空变化的影响较大,因此准确模拟云天太阳辐射的时空变化对电网安全运行至关重要。围绕如何减小中尺度气象模式的云初始场误差,进而改进云天的太阳辐射模拟这... 太阳能光伏发电已成为仅次于水电和风能的第三大可再生能源,光伏发电受云量时空变化的影响较大,因此准确模拟云天太阳辐射的时空变化对电网安全运行至关重要。围绕如何减小中尺度气象模式的云初始场误差,进而改进云天的太阳辐射模拟这一关键科学问题,本文通过研究基于卫星资料同化的LAPS(Local Analysis Prediction System)多时间层三维云分析同化方法,改进三维云结构,并将LAPS模式输出结果作为WRF(Weather Research and Forecasting)模式的初始场,模拟了2008年1月及夏季(6~8月)北京地区的总云量和总辐射的时空分布,重点分析了多云和有降水天气过程总辐射的模拟改进效果及其原因。结果表明,同化前后的总云量模拟值与观测值的时间变化趋势基本一致,但大部分时次总云量的模拟值低于观测值;大部分多云及降水时段同化后总云量模拟值较接近于实测值。1月晴天、多云天以及夏季晴天同化前后总辐射模拟值与实测值的时间变化趋势较一致,但同化前后两者的相关性差异不明显;晴天条件下同化前后总辐射模拟值均低于实测值,1月多云条件下多数时段同化后总辐射模拟误差减小不明显,与总云量的改进效果不显著有关。夏季多云、有降水及6月典型降水三种天气条件下同化前后总辐射模拟值与观测值的相关性稍差,同化后两者的相关性较同化前有所改进,尤其是6月典型降水过程改进效果较明显;同化前总辐射模拟误差较大,而同化后误差显著减小,尤其是6月典型降水过程同化后均方根误差和平均相对误差较同化前分别减小了102.6 W m^(-2)和355.9%,最大相对误差减小更显著;同化后总辐射模拟误差小于同化前的比例高达75%,即大部分时刻同化后模拟误差小于同化前。多云和有降水天气过程总辐射模拟效果的显著改进与总云量的改进密切相关,即同化后总云量模拟值增加,云的反射和散射作用增强,导致模拟总辐射减小,即更接近于实测总辐射值。研究结果对于多云和降水天气条件下太阳辐射的模拟效果改进、太阳能资源客观评估以及光伏电站的发电量预测具有一定的科学和实际应用价值。 展开更多
关键词 卫星资料同化 太阳辐射 光伏发电 太阳能 lapS—WRF模式系统
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中尺度分析系统LAPS应用雷达资料的个例研究 被引量:32
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作者 李红莉 崔春光 +2 位作者 王志斌 王晓芳 谢有才 《高原气象》 CSCD 北大核心 2009年第6期1443-1452,共10页
武汉暴雨所引进国际先进的中尺度分析系统LAPS,经成功本地移植后,该系统能融合华中区域多种非常规观测资料,提供包含丰富中尺度信息的高分辨率中尺度分析场。针对一次梅雨期强降水过程,利用LAPS系统进行中尺度分析,通过对比该系统融合... 武汉暴雨所引进国际先进的中尺度分析系统LAPS,经成功本地移植后,该系统能融合华中区域多种非常规观测资料,提供包含丰富中尺度信息的高分辨率中尺度分析场。针对一次梅雨期强降水过程,利用LAPS系统进行中尺度分析,通过对比该系统融合多普勒雷达资料前后各种物理量场的差异,研究了多普勒雷达资料在该系统中的作用。结果表明,LAPS融合雷达径向风后,对风场的改善比较明显,能揭示流场的中尺度信息。融合多种资料后的LAPS中尺度分析场比NCEP再分析场更接近实况,能清楚地反映中尺度特征,可作为分析此次强降水过程发生、发展的工具。融合雷达资料反射率后,LAPS云分析能提供与实况接近的较细致的云信息,有利于了解暴雨过程中云参数的演变,同时也为数值模式热启动提供条件。 展开更多
关键词 lapS系统 中尺度分析 云分析 多普勒雷达资料
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LAPS分析场资料在暴雨中尺度分析中的应用 被引量:28
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作者 周后福 郭品文 +2 位作者 翟菁 邱学兴 倪允琪 《高原气象》 CSCD 北大核心 2010年第2期461-470,共10页
利用常规地面探测、高密度自动雨量站、高空探测资料,并且融合多普勒雷达拼图产品,LAPS可输出比较精细的分析场。针对2008年6月21~22日一次暴雨天气过程,基于LAPS不同时空分辨率的中尺度分析场,分析了雷达回波、风场、高度场以及物理量... 利用常规地面探测、高密度自动雨量站、高空探测资料,并且融合多普勒雷达拼图产品,LAPS可输出比较精细的分析场。针对2008年6月21~22日一次暴雨天气过程,基于LAPS不同时空分辨率的中尺度分析场,分析了雷达回波、风场、高度场以及物理量场,比较不同时空分辨率LAPS分析场之间的差异。结果表明:(1)此次强降水过程可划分为2个阶段。第一阶段雨量集中在沿淮地区,呈纬向带状分布;第二阶段集中在大别山地区,其雨强更大,是中尺度局地性降水。(2)大别山区的地形抬升对低涡增强或减弱的演变具有重要影响,是低涡增强、发展的触发机制。当低涡接近大别山区时,低涡明显加强;当低涡逐渐远离大别山区时,低涡则减弱。(3)第一阶段有风的切变线,第二阶段有风的辐合,说明风场的辐合和切变促进云和降水的发生、发展,因此它们是产生暴雨的触发条件。(4)高分辨率的LAPS结果对于风场和云的变化刻画地更为细致。雷达回波发生在风的切变线处,或者风场辐合处;大别山区暴雨过程中,在其西侧有闭合性的涡旋,低涡缓慢东移,回波位于涡旋东侧,强降水发生在低涡东侧。(5)K指数、沙氏指数、整层可降水量与这次大别山地区暴雨过程具有较好的对应关系,与暴雨落区基本相同。这些指数在暴雨落区预报与应用方面具有一定参考价值。 展开更多
关键词 lapS分析场 暴雨过程 中尺度系统
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尿mAlb/Cr、NAG、LAP检测对早期糖尿病肾病的诊断价值 被引量:19
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作者 张晓青 宗成国 +1 位作者 栾旭华 赵黎明 《中国现代医学杂志》 CAS CSCD 北大核心 2009年第1期122-124,共3页
目的研究糖尿病患者的尿液微量白蛋白/肌酐(mAlb/Cr)、N-乙酰-β-D氨基葡萄糖苷酶(NAG)、亮氨酸氨基肽酶(LAP)与早期糖尿病肾病(DN)的诊断价值关系。方法将216例糖尿病患者分为两组:糖尿病无肾病组与早期糖尿病肾病组,采用免疫比浊法测... 目的研究糖尿病患者的尿液微量白蛋白/肌酐(mAlb/Cr)、N-乙酰-β-D氨基葡萄糖苷酶(NAG)、亮氨酸氨基肽酶(LAP)与早期糖尿病肾病(DN)的诊断价值关系。方法将216例糖尿病患者分为两组:糖尿病无肾病组与早期糖尿病肾病组,采用免疫比浊法测定尿微量白蛋白/肌酐,采用速率法检测尿NAG及LAP值,并与49例健康对照组比较。结果糖尿病肾病患者尿mAlb/Cr、NAG、LAP明显高于对照组(P<0.01),且尿微量白蛋白/肌酐与NAG,LAP成正相关(r=0.909,r=0.410,P<0.01)。结论检测尿mAlb/Cr、NAG、LAP有助于尿蛋白阴性的糖尿病肾病的早期诊断。 展开更多
关键词 mAlb/Cr NAG lap 糖尿病肾病
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LAPS与STMAS地面气温融合效果对比试验 被引量:30
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作者 张涛 苗春生 王新 《高原气象》 CSCD 北大核心 2014年第3期743-752,共10页
利用局地分析和预报系统LAPS(Local Analysis and Prediction System)和多时空尺度分析系统STMAS(Space Time Multiscale Analysis System)对北京、华北和中国3个不同区域的区域自动站观测资料和全球预报系统GFS(Global Forecast System... 利用局地分析和预报系统LAPS(Local Analysis and Prediction System)和多时空尺度分析系统STMAS(Space Time Multiscale Analysis System)对北京、华北和中国3个不同区域的区域自动站观测资料和全球预报系统GFS(Global Forecast System)背景场资料,在1,5,10和20 km等4种分辨率的网格上进行融合试验,从算法的角度对比分析LAPS和STMAS在融合效果上的差异,并利用国家级自动站数据对LAPS和STMAS一年内逐时融合结果进行独立性检验。结果表明,在东部观测密集区,LAPS和STMAS都有不错的表现,STMAS能够解析出观测中细小的特征,融合结果更接近实际观测,相对而言LAPS则平滑作用明显,容易损失观测信息;在资料稀疏地区,STMAS的优势更为明显,由粗网格到细网格逐层分尺度进行分析,使粗网格尺度的误差得到快速修正,避免不同尺度观测信息的混淆,青藏高原等地区的融合结果得到有效改善。此外,地形高度调整的不合理性对LAPS、STMAS在山区的融合效果有所影响。 展开更多
关键词 lapS STMAS 地面气温 数据融合
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