Set against the cultural and historical backdrop of 18th-century Venice,Giulia Elisabetta Lama’s(1681-1747)remarkable paintings reveal intriguing connections to mythology and science.Lama’s notable depiction of Satu...Set against the cultural and historical backdrop of 18th-century Venice,Giulia Elisabetta Lama’s(1681-1747)remarkable paintings reveal intriguing connections to mythology and science.Lama’s notable depiction of Saturn Devouring His Child,painted in oils in 1729 and now in a private collection,draws significant attention.展开更多
目的探讨LAMA4调控TGF-β1/SMAD Western blot检测LAMA4在肝癌细胞Huh7和HepG2及正常肝细胞L02中的表达;MMT法和流式细胞技术检测LAMA4和TGF-β1/SMAD信号传导途径对Huh7细胞增殖和凋亡的影响;RT-qPCR和Western blot检测LAMA4对TGF-β1/...目的探讨LAMA4调控TGF-β1/SMAD Western blot检测LAMA4在肝癌细胞Huh7和HepG2及正常肝细胞L02中的表达;MMT法和流式细胞技术检测LAMA4和TGF-β1/SMAD信号传导途径对Huh7细胞增殖和凋亡的影响;RT-qPCR和Western blot检测LAMA4对TGF-β1/SMAD信号转导通路和细胞免疫因子TNF-α、IFN-γ、TGF-β1的影响。结果 RT-qPCR和Western blot检测结果显示:LAMA4在肝癌细胞Huh7和HepG2中mRNA表达显著上调;LAMA4在肝癌细胞Huh7和HepG2中蛋白表达显著上调;沉默LAMA4使TGF-β1和SMAD4表达显著上调,SMAD6、IFN-γ和TNF-α的含量显著降低,过表达LAMA4则结果相反;MMT法和流式细胞技术检测结果显示:敲低LAMA4可使Huh7细胞活力下降,细胞凋亡率增加,而过表达LAMA4则结果相反;Ly364947干扰TGF-β1/SMAD信号传导路径后,Huh7细胞的活力增加,细胞凋亡率显著下降,IFN-γ和TNF-α的含量显著升高,TGF-β1显著降低,且Ly364947可部分逆转沉默LAMA4对肝癌细胞Huh7的免疫逃逸因子和凋亡的调控作用。结论 LAMA4可通过调控TGF-β1/SMAD信号传导途径影响肝癌细胞免疫逃逸和凋亡。展开更多
Functional studies have suggested the important role of early growth response I （EGR1） and Laminin a2- chain （LAMA,？.） in human eye development. Genetic studies have reported a significant association of the sing...Functional studies have suggested the important role of early growth response I （EGR1） and Laminin a2- chain （LAMA,？.） in human eye development. Genetic studies have reported a significant association of the single nu- cleotide polymorphism （SNP） in the LAMA2 gene with myopia. This study aimed to evaluate the association of the tagging SNPs （tSNPs） in the EGR1 and LAMA2 genes with high myopia in two independent Han Chinese populations. Four tSNPs （rs11743810 in the EGR1 gene; rs2571575, rs9321170, and rs1889891 in the LAMA2 gene） were se- lected, according to the HapMap database （http：//hapmap.ncbi.nlm.nih.gov）, and were genotyped using the ligase detection reaction （LDR） approach for 167 Hart Chinese nuclear families with extremely highly myopic offspring （〈-10.0 diopters） and an independent group with 485 extremely highly myopic cases （〈-10.0 diopters） and 499 con- trois. Direct sequencing was used to confirm the LDR results in twenty randomly selected subjects. Family-based association analysis was performed using the family-based association test （FBAT） software package （Version 1.5.5）. Population-based association analysis was performed using the Chi-square test. The association analysis power was estimated using online software （http：lldesign.cs.ucla.edu）. The FBAT demonstrated that all four tSNPs tested did not show association with high myopia （P〉0.05）. Haplotype analysis of tSNPs in the LAMA2 genes also did not show a significant association （P〉0.05）. Meanwhile, population-based association analysis also showed no significant asso- ciation results with high myopia （P〉0.05）. On the basis of our family- and population-based analyses for the Han Chinese population, we did not find positive association signals of the four SNPs in the LAMA2 and EGR1 genes with high myopia.展开更多
文摘Set against the cultural and historical backdrop of 18th-century Venice,Giulia Elisabetta Lama’s(1681-1747)remarkable paintings reveal intriguing connections to mythology and science.Lama’s notable depiction of Saturn Devouring His Child,painted in oils in 1729 and now in a private collection,draws significant attention.
基金Project supported by the Natural Science Foundation of Zhejiang Province(No.LY14H120003)the Qianjiang Talent Project of Zhejiang Province(No.2013R10040),China
文摘Functional studies have suggested the important role of early growth response I （EGR1） and Laminin a2- chain （LAMA,？.） in human eye development. Genetic studies have reported a significant association of the single nu- cleotide polymorphism （SNP） in the LAMA2 gene with myopia. This study aimed to evaluate the association of the tagging SNPs （tSNPs） in the EGR1 and LAMA2 genes with high myopia in two independent Han Chinese populations. Four tSNPs （rs11743810 in the EGR1 gene; rs2571575, rs9321170, and rs1889891 in the LAMA2 gene） were se- lected, according to the HapMap database （http：//hapmap.ncbi.nlm.nih.gov）, and were genotyped using the ligase detection reaction （LDR） approach for 167 Hart Chinese nuclear families with extremely highly myopic offspring （〈-10.0 diopters） and an independent group with 485 extremely highly myopic cases （〈-10.0 diopters） and 499 con- trois. Direct sequencing was used to confirm the LDR results in twenty randomly selected subjects. Family-based association analysis was performed using the family-based association test （FBAT） software package （Version 1.5.5）. Population-based association analysis was performed using the Chi-square test. The association analysis power was estimated using online software （http：lldesign.cs.ucla.edu）. The FBAT demonstrated that all four tSNPs tested did not show association with high myopia （P〉0.05）. Haplotype analysis of tSNPs in the LAMA2 genes also did not show a significant association （P〉0.05）. Meanwhile, population-based association analysis also showed no significant asso- ciation results with high myopia （P〉0.05）. On the basis of our family- and population-based analyses for the Han Chinese population, we did not find positive association signals of the four SNPs in the LAMA2 and EGR1 genes with high myopia.
