长链酰基辅酶A合成酶(long-chain acyl-coenzyme A synthetase,LACS)在脂肪酸代谢、角质层角质和蜡的合成中发挥着重要作用。为研究小麦中LACS基因的特征,以中国春为材料,进行全基因组鉴定和分析。结果共鉴定出148个小麦LACS基因(TaLAC...长链酰基辅酶A合成酶(long-chain acyl-coenzyme A synthetase,LACS)在脂肪酸代谢、角质层角质和蜡的合成中发挥着重要作用。为研究小麦中LACS基因的特征,以中国春为材料,进行全基因组鉴定和分析。结果共鉴定出148个小麦LACS基因(TaLACS),不均匀分布于21条染色体上,且有成簇分布的现象,编码蛋白大部分为中性、亲水的水溶性蛋白,性质稳定。系统进化分析结果显示,148个TaLACS基因聚为6组,有55对旁系同源基因。表达谱分析表明,TaLACS基因在不同组织和逆境胁迫下存在明显的差异表达,说明TaLACS基因具有组织特异性,在抗低温、干旱和赤霉病过程中TaLACS基因发挥重要作用。该家族中含有响应低温、干旱和脱落酸元件的基因分别占34.9%、46.0%和85.8%,揭示TaLACS基因参与生物胁迫和非生物胁迫过程。展开更多
Long-chain acyl-Coenzyme A （CoA） synthetases （LACSs） catalyze the formation of long-chain acyl-CoA, and play important roles in fatty acid metabolism including phospholipids, triacylglycerol （TAG） biosynthesis a...Long-chain acyl-Coenzyme A （CoA） synthetases （LACSs） catalyze the formation of long-chain acyl-CoA, and play important roles in fatty acid metabolism including phospholipids, triacylglycerol （TAG） biosynthesis and fatty acid 13-oxidation. Here, we report the characterization of a LACS gene from Brassica napus. It is highly homologous to Arabidopsis LACS4 and thus designated as BnLACS4. The cloned gene BnLACS4 could complement a LACS-deficient yeast strain YB525. It is mainly expressed in flowers and developing seeds where lipid biosynthesis is at high rate in Brassiea napus. When transiently expressed in tobacco leaves, BnLACS4 is localized in endoplasmic reticulum （ER）, the common site for eukaryotic pathway of lipid biosynthesis. Expression of BnLACS4 in the yeast strain pep4 increased its lipid content. Taken together, our results suggest that BnLACS4 may be involved in lipid biosynthesis in B. napus.展开更多
The arbitrary discharge of tetracycline(TC)residuals has seriously influenced the ecosystem and human health.Laccase(Lac)-based biodegradation technology is considered a more effective way to remove TC due to its high...The arbitrary discharge of tetracycline(TC)residuals has seriously influenced the ecosystem and human health.Laccase(Lac)-based biodegradation technology is considered a more effective way to remove TC due to its high catalytic efficiency and less by-product.Nevertheless,free Lac suffers from poor stability,easy inactivation and difficult recovery,restricting its application.Immobilization of Lac is considered an efficient strategy for addressing these obstacles.In this study,a magnetic metal-organic framework of Fe_(3)O_(4)@SiO_(2)@UiO-66-NH_(2)(MMOF)was prepared and used as a carrier to immobilize Lac(Lac@MMOF)for TC degradation.Benefiting from the multiple binding sites,adsorption,and protection effect of MMOF,Lac@MMOF displayed a wider pH application range(2–7)and better thermal(15–85℃),repeatability,and storage stability than free Lac.Furthermore,owing to the synergism of MOF adsorption and Lac biocatalysis,the removal rate of Lac@MMOF for TC could be up to 98%at pH=7 within 1 hr,which was 1.29 and 1.24 times that of free Lac and MMOF,respectively.More importantly,Lac@MMOF could easily be separated from aqueous solution under a magnetic field and maintained good removal performance(80%)after five cycles.The degradation products were identified by applying LC-MS/MS,and possible degradation mechanisms and pathways were proposed.Finally,the antibacterial activity of intermediate products was evaluated using Escherichia coli,which revealed that the toxicity of TC was reduced effectively by the degradation of Lac@MMOF.Overall,Lac@MMOF is a green alternative for residual antibiotic removal in water.展开更多
On September 1st,the 18th Chi na-LAC Business Summit Promotion Conference opened in Beijing.Chinese and foreign vips gathered to prepare for the 18th China LAC Business Summit,planned for Zhengzhou,Henan,from Novemb...On September 1st,the 18th Chi na-LAC Business Summit Promotion Conference opened in Beijing.Chinese and foreign vips gathered to prepare for the 18th China LAC Business Summit,planned for Zhengzhou,Henan,from November 3rd to 4th.展开更多
Zhengzhou,located at the eastern foot of Mount Song and along the banks of the Yel low River,sits at the heart of China.It is the capital of Henan Province and one of China’s eight great ancient capitals.Historically...Zhengzhou,located at the eastern foot of Mount Song and along the banks of the Yel low River,sits at the heart of China.It is the capital of Henan Province and one of China’s eight great ancient capitals.Historically known as the“Center of Heaven and Earth”,it is now praised as the“Green City”As a national central city expl icit ly suppor ted by the Chinese government and a core growth pole for high-quality regional development.展开更多
文摘长链酰基辅酶A合成酶(long-chain acyl-coenzyme A synthetase,LACS)在脂肪酸代谢、角质层角质和蜡的合成中发挥着重要作用。为研究小麦中LACS基因的特征,以中国春为材料,进行全基因组鉴定和分析。结果共鉴定出148个小麦LACS基因(TaLACS),不均匀分布于21条染色体上,且有成簇分布的现象,编码蛋白大部分为中性、亲水的水溶性蛋白,性质稳定。系统进化分析结果显示,148个TaLACS基因聚为6组,有55对旁系同源基因。表达谱分析表明,TaLACS基因在不同组织和逆境胁迫下存在明显的差异表达,说明TaLACS基因具有组织特异性,在抗低温、干旱和赤霉病过程中TaLACS基因发挥重要作用。该家族中含有响应低温、干旱和脱落酸元件的基因分别占34.9%、46.0%和85.8%,揭示TaLACS基因参与生物胁迫和非生物胁迫过程。
基金supported by the Natural Science Foundation of China(31271760)the Opening Funds of the Key Laboratory of Biology and Genetic Improvement of Oilcrops,Ministry of Agriculture,China
文摘Long-chain acyl-Coenzyme A （CoA） synthetases （LACSs） catalyze the formation of long-chain acyl-CoA, and play important roles in fatty acid metabolism including phospholipids, triacylglycerol （TAG） biosynthesis and fatty acid 13-oxidation. Here, we report the characterization of a LACS gene from Brassica napus. It is highly homologous to Arabidopsis LACS4 and thus designated as BnLACS4. The cloned gene BnLACS4 could complement a LACS-deficient yeast strain YB525. It is mainly expressed in flowers and developing seeds where lipid biosynthesis is at high rate in Brassiea napus. When transiently expressed in tobacco leaves, BnLACS4 is localized in endoplasmic reticulum （ER）, the common site for eukaryotic pathway of lipid biosynthesis. Expression of BnLACS4 in the yeast strain pep4 increased its lipid content. Taken together, our results suggest that BnLACS4 may be involved in lipid biosynthesis in B. napus.
文摘为探讨缢蛏(Sinonovacula constricta)耐受高硫化物环境的分子机理,利用RNA-seq技术开展395μmol/L硫化物胁迫0 h、6 h和96 h缢蛏鳃转录组动态变化的研究,构建9个转录组文库,获得27.09 Gb有效数据,组装得到65747条Unigenes,平均长度为971.46 bp,N50达到1434 bp,注释得到22921条Unigenes。按照FPKM值获得基因表达丰度后,采用DEGseq软件进行差异表达分析,分别在6 h vs.0 h、96 h vs.0 h和96 h vs.6 h组中获得61138、60912和62824条DEGs。按照DEGs筛选阈值,分别在6 h vs.0 h、96 h vs.0 h和96 h vs.6 h组中获得17、10和12个高表达DEGs。KEGG分析显示差异基因(DEGs)显著富集到硫代谢通路,发现高表达的硫醌氧化还原酶基因(SQR),推测线粒体硫化物氧化是其解毒的关键。GO、KEGG分析发现DEGs分别富集到呼吸电子传递链(ETC)及氧化磷酸化通路,细胞色素b的表达显著下调,可能引发线粒体氧化呼吸链受阻,从而产生过量ROS,以激活机体的应激反应。DEGs还富集到转移含磷基团的转移酶活性、核小体组装等GO类别,推测ROS造成DNA氧化损伤后,机体通过DNA复制、组装新染色体以维持遗传物质稳定。采用qRT-PCR进行差异基因组蛋白(H2B)、漆酶(LAC)的响应研究,结果呈时间依赖性的表达模式,推测H2B通过修复受损DNA并增强免疫能力、LAC通过清除ROS以提高机体硫化物抗性。研究结果系统解析了缢蛏耐硫的分子调控机理,为缢蛏耐硫新品种的遗传育种研究奠定了理论基础。
基金supported by the National Natural Science Foundation of China(No.U20A20133)the National Key Research and Development Program of China(No.2022YFF0606703).
文摘The arbitrary discharge of tetracycline(TC)residuals has seriously influenced the ecosystem and human health.Laccase(Lac)-based biodegradation technology is considered a more effective way to remove TC due to its high catalytic efficiency and less by-product.Nevertheless,free Lac suffers from poor stability,easy inactivation and difficult recovery,restricting its application.Immobilization of Lac is considered an efficient strategy for addressing these obstacles.In this study,a magnetic metal-organic framework of Fe_(3)O_(4)@SiO_(2)@UiO-66-NH_(2)(MMOF)was prepared and used as a carrier to immobilize Lac(Lac@MMOF)for TC degradation.Benefiting from the multiple binding sites,adsorption,and protection effect of MMOF,Lac@MMOF displayed a wider pH application range(2–7)and better thermal(15–85℃),repeatability,and storage stability than free Lac.Furthermore,owing to the synergism of MOF adsorption and Lac biocatalysis,the removal rate of Lac@MMOF for TC could be up to 98%at pH=7 within 1 hr,which was 1.29 and 1.24 times that of free Lac and MMOF,respectively.More importantly,Lac@MMOF could easily be separated from aqueous solution under a magnetic field and maintained good removal performance(80%)after five cycles.The degradation products were identified by applying LC-MS/MS,and possible degradation mechanisms and pathways were proposed.Finally,the antibacterial activity of intermediate products was evaluated using Escherichia coli,which revealed that the toxicity of TC was reduced effectively by the degradation of Lac@MMOF.Overall,Lac@MMOF is a green alternative for residual antibiotic removal in water.
文摘On September 1st,the 18th Chi na-LAC Business Summit Promotion Conference opened in Beijing.Chinese and foreign vips gathered to prepare for the 18th China LAC Business Summit,planned for Zhengzhou,Henan,from November 3rd to 4th.
文摘Zhengzhou,located at the eastern foot of Mount Song and along the banks of the Yel low River,sits at the heart of China.It is the capital of Henan Province and one of China’s eight great ancient capitals.Historically known as the“Center of Heaven and Earth”,it is now praised as the“Green City”As a national central city expl icit ly suppor ted by the Chinese government and a core growth pole for high-quality regional development.
