Long-chain acyl-CoA synthetase(LACS),belonging to the AMP-bound superfamily,catalyzes the activation of long-chain fatty acids to their coenzyme A esters.Candidate Gene Association analysis showed that LACS8 was not o...Long-chain acyl-CoA synthetase(LACS),belonging to the AMP-bound superfamily,catalyzes the activation of long-chain fatty acids to their coenzyme A esters.Candidate Gene Association analysis showed that LACS8 was not only related to the content of fatty acid,but also,surprisingly,to the content of glucosinolates.Here,we cloned BnaLACS8A03 from rapeseed,and demonstrated its association with the glucosinolates content.We found that BnaLACS8A03 was widely expressed in all tissues and organs,and significantly highly expressed in leaf and flower,which are the sites of glucosinolate synthesis.Bioinformatics predicted that it was located in peroxisome,where theβ-oxidation of fatty acids mainly occurred,indicating that BnaLACS8A03 was involved in the metabolism of fatty acids.Phenotypic studies of RNA interference(RNAi)lines revealed that down-regulation of BnaLACS8A03 in rapeseed significantly reduced the glucosinolates content.In addition,the expression of glucosinolates biosynthesis-related genes and transport-related genes is altered in BnaLACS8A03-RNAi plants,suggesting that a possible crosstalk regulation mechanism exists in the regulation network of glucosinolate mechanism.Our results demonstrate that glucosinolates content can be manipulated by altering the expression of long chain acyl-Coenzyme A synthase gene BnaLACS8A03,which provide an available genetic resource for low glucosinolate breeding practice in rapeseed.展开更多
The arbitrary discharge of tetracycline(TC)residuals has seriously influenced the ecosystem and human health.Laccase(Lac)-based biodegradation technology is considered a more effective way to remove TC due to its high...The arbitrary discharge of tetracycline(TC)residuals has seriously influenced the ecosystem and human health.Laccase(Lac)-based biodegradation technology is considered a more effective way to remove TC due to its high catalytic efficiency and less by-product.Nevertheless,free Lac suffers from poor stability,easy inactivation and difficult recovery,restricting its application.Immobilization of Lac is considered an efficient strategy for addressing these obstacles.In this study,a magnetic metal-organic framework of Fe_(3)O_(4)@SiO_(2)@UiO-66-NH_(2)(MMOF)was prepared and used as a carrier to immobilize Lac(Lac@MMOF)for TC degradation.Benefiting from the multiple binding sites,adsorption,and protection effect of MMOF,Lac@MMOF displayed a wider pH application range(2–7)and better thermal(15–85℃),repeatability,and storage stability than free Lac.Furthermore,owing to the synergism of MOF adsorption and Lac biocatalysis,the removal rate of Lac@MMOF for TC could be up to 98%at pH=7 within 1 hr,which was 1.29 and 1.24 times that of free Lac and MMOF,respectively.More importantly,Lac@MMOF could easily be separated from aqueous solution under a magnetic field and maintained good removal performance(80%)after five cycles.The degradation products were identified by applying LC-MS/MS,and possible degradation mechanisms and pathways were proposed.Finally,the antibacterial activity of intermediate products was evaluated using Escherichia coli,which revealed that the toxicity of TC was reduced effectively by the degradation of Lac@MMOF.Overall,Lac@MMOF is a green alternative for residual antibiotic removal in water.展开更多
On September 1st,the 18th Chi na-LAC Business Summit Promotion Conference opened in Beijing.Chinese and foreign vips gathered to prepare for the 18th China LAC Business Summit,planned for Zhengzhou,Henan,from Novemb...On September 1st,the 18th Chi na-LAC Business Summit Promotion Conference opened in Beijing.Chinese and foreign vips gathered to prepare for the 18th China LAC Business Summit,planned for Zhengzhou,Henan,from November 3rd to 4th.展开更多
Zhengzhou,located at the eastern foot of Mount Song and along the banks of the Yel low River,sits at the heart of China.It is the capital of Henan Province and one of China’s eight great ancient capitals.Historically...Zhengzhou,located at the eastern foot of Mount Song and along the banks of the Yel low River,sits at the heart of China.It is the capital of Henan Province and one of China’s eight great ancient capitals.Historically known as the“Center of Heaven and Earth”,it is now praised as the“Green City”As a national central city expl icit ly suppor ted by the Chinese government and a core growth pole for high-quality regional development.展开更多
In this study,the apsidal motion analyses of the eclipsing binary systems V398 Lac,V2544 Cyg,and V785 Cas are presented.The(O-C)diagrams of the selected systems were constructed using all available and reliable times ...In this study,the apsidal motion analyses of the eclipsing binary systems V398 Lac,V2544 Cyg,and V785 Cas are presented.The(O-C)diagrams of the selected systems were constructed using all available and reliable times of minimum light compiled from the literature.As a result,the apsidal motion periods were determined to be 353±50 yr,43.3±5.1 yr and 83.4±8.5 yr,respectively.Furthermore,the TESS light curves of the three systems were analyzed to derive their photometric and absolute parameters.The component masses were determined as follows:for V398 Lac,M_(1)=3.83±0.35 M_(⊙)and M2=3.27±0.35 M_(⊙);for V2544 Cyg,M_(1)=1.75±0.38 M_(⊙)and M2=1.40±0.38 M_(⊙);for V785 Cas,M_(1)=5.64±0.41 M_(⊙)and M2=5.18±0.41 M_(⊙).The parameters related to apsidal motion,as well as the observational and theoretical internal structure constants(K_(2,obs) and K_(2,teo))were calculated for studied binaries.The relativistic contribution to the observed apsidal motion rate was found to be relatively small,constituting less than approximately 5%of the total rate in each system.The theoretical internal structure constants were derived from the evolutionary models,assuming a standard chemical composition of(X,Z)=(0.70,0.01).展开更多
基金supported by the National Key R&D Program of China(2016YFD0101900 and 2016YFD0100305)the National Natural Science Foundation of China(Grant No.32001582)the General Project of Natural Science Research in Colleges and Universities of Jiangsu Province(Grant No.20KJB210002)。
文摘Long-chain acyl-CoA synthetase(LACS),belonging to the AMP-bound superfamily,catalyzes the activation of long-chain fatty acids to their coenzyme A esters.Candidate Gene Association analysis showed that LACS8 was not only related to the content of fatty acid,but also,surprisingly,to the content of glucosinolates.Here,we cloned BnaLACS8A03 from rapeseed,and demonstrated its association with the glucosinolates content.We found that BnaLACS8A03 was widely expressed in all tissues and organs,and significantly highly expressed in leaf and flower,which are the sites of glucosinolate synthesis.Bioinformatics predicted that it was located in peroxisome,where theβ-oxidation of fatty acids mainly occurred,indicating that BnaLACS8A03 was involved in the metabolism of fatty acids.Phenotypic studies of RNA interference(RNAi)lines revealed that down-regulation of BnaLACS8A03 in rapeseed significantly reduced the glucosinolates content.In addition,the expression of glucosinolates biosynthesis-related genes and transport-related genes is altered in BnaLACS8A03-RNAi plants,suggesting that a possible crosstalk regulation mechanism exists in the regulation network of glucosinolate mechanism.Our results demonstrate that glucosinolates content can be manipulated by altering the expression of long chain acyl-Coenzyme A synthase gene BnaLACS8A03,which provide an available genetic resource for low glucosinolate breeding practice in rapeseed.
基金supported by the National Natural Science Foundation of China(No.U20A20133)the National Key Research and Development Program of China(No.2022YFF0606703).
文摘The arbitrary discharge of tetracycline(TC)residuals has seriously influenced the ecosystem and human health.Laccase(Lac)-based biodegradation technology is considered a more effective way to remove TC due to its high catalytic efficiency and less by-product.Nevertheless,free Lac suffers from poor stability,easy inactivation and difficult recovery,restricting its application.Immobilization of Lac is considered an efficient strategy for addressing these obstacles.In this study,a magnetic metal-organic framework of Fe_(3)O_(4)@SiO_(2)@UiO-66-NH_(2)(MMOF)was prepared and used as a carrier to immobilize Lac(Lac@MMOF)for TC degradation.Benefiting from the multiple binding sites,adsorption,and protection effect of MMOF,Lac@MMOF displayed a wider pH application range(2–7)and better thermal(15–85℃),repeatability,and storage stability than free Lac.Furthermore,owing to the synergism of MOF adsorption and Lac biocatalysis,the removal rate of Lac@MMOF for TC could be up to 98%at pH=7 within 1 hr,which was 1.29 and 1.24 times that of free Lac and MMOF,respectively.More importantly,Lac@MMOF could easily be separated from aqueous solution under a magnetic field and maintained good removal performance(80%)after five cycles.The degradation products were identified by applying LC-MS/MS,and possible degradation mechanisms and pathways were proposed.Finally,the antibacterial activity of intermediate products was evaluated using Escherichia coli,which revealed that the toxicity of TC was reduced effectively by the degradation of Lac@MMOF.Overall,Lac@MMOF is a green alternative for residual antibiotic removal in water.
文摘On September 1st,the 18th Chi na-LAC Business Summit Promotion Conference opened in Beijing.Chinese and foreign vips gathered to prepare for the 18th China LAC Business Summit,planned for Zhengzhou,Henan,from November 3rd to 4th.
文摘Zhengzhou,located at the eastern foot of Mount Song and along the banks of the Yel low River,sits at the heart of China.It is the capital of Henan Province and one of China’s eight great ancient capitals.Historically known as the“Center of Heaven and Earth”,it is now praised as the“Green City”As a national central city expl icit ly suppor ted by the Chinese government and a core growth pole for high-quality regional development.
基金Funding for TESS is provided by the NASA Science Mission Directorate。
文摘In this study,the apsidal motion analyses of the eclipsing binary systems V398 Lac,V2544 Cyg,and V785 Cas are presented.The(O-C)diagrams of the selected systems were constructed using all available and reliable times of minimum light compiled from the literature.As a result,the apsidal motion periods were determined to be 353±50 yr,43.3±5.1 yr and 83.4±8.5 yr,respectively.Furthermore,the TESS light curves of the three systems were analyzed to derive their photometric and absolute parameters.The component masses were determined as follows:for V398 Lac,M_(1)=3.83±0.35 M_(⊙)and M2=3.27±0.35 M_(⊙);for V2544 Cyg,M_(1)=1.75±0.38 M_(⊙)and M2=1.40±0.38 M_(⊙);for V785 Cas,M_(1)=5.64±0.41 M_(⊙)and M2=5.18±0.41 M_(⊙).The parameters related to apsidal motion,as well as the observational and theoretical internal structure constants(K_(2,obs) and K_(2,teo))were calculated for studied binaries.The relativistic contribution to the observed apsidal motion rate was found to be relatively small,constituting less than approximately 5%of the total rate in each system.The theoretical internal structure constants were derived from the evolutionary models,assuming a standard chemical composition of(X,Z)=(0.70,0.01).
