State Key Laboratory of Baiyun Obo Rare Earth Resource Researches and Comprehensive Utilization was approved by the Ministry of Science and Technology to be one of the national key laboratories in November 2022.
Objective This study reports the first imported case of Lassa fever(LF)in China.Laboratory detection and molecular epidemiological analysis of the Lassa virus(LASV)from this case offer valuable insights for the preven...Objective This study reports the first imported case of Lassa fever(LF)in China.Laboratory detection and molecular epidemiological analysis of the Lassa virus(LASV)from this case offer valuable insights for the prevention and control of LF.Methods Samples of cerebrospinal fluid(CSF),blood,urine,saliva,and environmental materials were collected from the patient and their close contacts for LASV nucleotide detection.Whole-genome sequencing was performed on positive samples to analyze the genetic characteristics of the virus.Results LASV was detected in the patient’s CSF,blood,and urine,while all samples from close contacts and the environment tested negative.The virus belongs to the lineage IV strain and shares the highest homology with strains from Sierra Leone.The variability in the glycoprotein complex(GPC)among different strains ranged from 3.9%to 15.1%,higher than previously reported for the seven known lineages.Amino acid mutation analysis revealed multiple mutations within the GPC immunogenic epitopes,increasing strain diversity and potentially impacting immune response.Conclusion The case was confirmed through nucleotide detection,with no evidence of secondary transmission or viral spread.The LASV strain identified belongs to lineage IV,with broader GPC variability than previously reported.Mutations in the immune-related sites of GPC may affect immune responses,necessitating heightened vigilance regarding the virus.展开更多
State Key Laboratory of Baiyun Obo Rare Earth Resource Researches and Comprehensive Utilization was approved by the Ministry of Science and Technology to be one of the national key laboratories in November 2022.The la...State Key Laboratory of Baiyun Obo Rare Earth Resource Researches and Comprehensive Utilization was approved by the Ministry of Science and Technology to be one of the national key laboratories in November 2022.The laboratory was reconstructed based on former State Key Laboratory of Baiyun Obo Rare Earth Resources Researches and Comprehensive Utilization.展开更多
Background:Medical laboratory diagnostic services play a critical role in the diagnosis,treatment,and manage-ment of diseases,forming the cornerstone of effective healthcare systems.Despite the crucial role of laborat...Background:Medical laboratory diagnostic services play a critical role in the diagnosis,treatment,and manage-ment of diseases,forming the cornerstone of effective healthcare systems.Despite the crucial role of laboratory services,the quality and accessibility of medical laboratory services in sub-Saharan Africa(SSA)face signifi-cant challenges.Global health development aid has been pivotal in supporting SSA laboratory services.This study aimed to examine global health development aid initiatives that have successfully enhanced the quality of medical laboratory services and the challenges and barriers to effectively improving medical laboratory services through global health development aid in SSA.Methods:We used a narrative review study design.We searched PubMed,Web of Science and Scopus for articles published in the last 15 years.These three databases are generally considered premier databases for peer-reviewed articles in global health,public health,health systems,and biomedical sciences.The inclusion criteria for this review included research studies,reports,and grey literature.Only articles published in English from 2010 on-ward were considered.The analysis followed a qualitative approach,emphasizing thematic synthesis and critical interpretation.Results:Forty articles were included in this study.Of these,18 were primary research studies,11 were reports,7 were commentaries,and 4 were reviews.Five sub-themes from the successful global health development aid initiative themes were capacity building and training programs,infrastructure development,partnership models,policy advocacy and regulatory support,quality control and standardization of laboratory services.The sub-themes from the challenges and barrier theme were insufficient funding and resource allocation,human resource constraints,inadequate infrastructure and equipment,and political and institutional barriers.This review re-vealed that several factors,including financial sustainability,human resource capacity,institutional support,resilience,and effective monitoring systems,shape the sustainability of improvements in medical laboratory services in SSA.Conclusion:Achieving long-term sustainability requires strategies that ensure financial self-sufficiency,foster a skilled and stable workforce,and integrate laboratory services into national health frameworks.展开更多
This study aims to understand the effect of injection rate on injection-induced fracture activation in granite.We performed water injection-induced slip tests on samples containing either a smooth or a rough fracture ...This study aims to understand the effect of injection rate on injection-induced fracture activation in granite.We performed water injection-induced slip tests on samples containing either a smooth or a rough fracture at four different injection rates under undrained conditions and monitored the acoustic emission(AE)signals during the tests.Experimental results reveal that the critical activation fluid pressure is related to the injection rate,pressure diffusion rate,stress state,and fracture roughness.For the smooth fracture,as the injection rate increases,the critical activation fluid pressure increases significantly,while the injection rate has little effect on the critical activation fluid pressure of the rough fracture.The quasi-static slip distance of fractures decreases as the injection rate increases,with rough fractures exhibiting a greater overall slip distance compared to smooth fractures.The number of AE events per unit sliding distance increases with the injection rate,while the global b value decreases.These results indicate that higher injection rates produce more large-magnitude AE events and more severe slip instability and asperity damage.We established a linkage between fluid injection volume,injection rate,and AE events using the seismogenic index(Σ).The smooth fracture exhibits a steadily increasingΣwith the elapse of injection time,and the rate of increase is higher at higher injection rates;while the rough fracture is featured by a fluctuatingΣ,signifying the intermittent occurrence of large-magnitude AE events associated with the damage of larger fracture asperities.Our results highlight the importance of fracture surface heterogeneity on injection-induced fracture activation and slip.展开更多
With the continuous advancement of the tiered diagnosis and treatment system,the medical consortium model has gained increasing attention as an important approach to promoting the vertical integration of healthcare re...With the continuous advancement of the tiered diagnosis and treatment system,the medical consortium model has gained increasing attention as an important approach to promoting the vertical integration of healthcare resources.Within this context,laboratory data,as a key component of healthcare information systems,urgently requires efficient sharing and intelligent analysis.This paper designs and constructs an intelligent early warning system for laboratory data based on a cloud platform tailored to the medical consortium model.Through standardized data formats and unified access interfaces,the system enables the integration and cleaning of laboratory data across multiple healthcare institutions.By combining medical rule sets with machine learning models,the system achieves graded alerts and rapid responses to abnormal key indicators and potential outbreaks of infectious diseases.Practical deployment results demonstrate that the system significantly improves the utilization efficiency of laboratory data,strengthens public health event monitoring,and optimizes inter-institutional collaboration.The paper also discusses challenges encountered during system implementation,such as inconsistent data standards,security and compliance concerns,and model interpretability,and proposes corresponding optimization strategies.These findings provide a reference for the broader application of intelligent medical early warning systems.展开更多
Against the backdrop of the rapid intelligent development of medical laboratory technology,vocational education is in urgent need of teaching innovation.This paper explores the integration and practice of information-...Against the backdrop of the rapid intelligent development of medical laboratory technology,vocational education is in urgent need of teaching innovation.This paper explores the integration and practice of information-based teaching methods in vocational education of medical laboratory technology,analyzes the current situation of traditional teaching and the demand for informatization,and elaborates on the practical paths from aspects such as resource construction,model innovation,and evaluation reform.Practice has verified that this integration has improved teaching quality and students’skills,but there are problems such as uneven resources and the need to improve teachers’and students’abilities.Countermeasures such as strengthening co-construction and sharing of resources and carrying out special training are proposed to provide reference for the development of professional education.展开更多
The clinical application of solid lipid particles(SLPs)is hampered due to the need for advanced nano/micro-suspension production technology.This research aims to establish a pilot-scale production line employing high-...The clinical application of solid lipid particles(SLPs)is hampered due to the need for advanced nano/micro-suspension production technology.This research aims to establish a pilot-scale production line employing high-speed shears as emulsification equipment.The primary purpose is to manufacture nano/micro-suspensions using solid lipid particles(SLPs).The study also exhaustively introduces and analyzes the regulatory schemes for process parameters and formulations at various stages of production.The process and formulation endured optimization through orthog-onal or single-factor tests at various production steps:laboratory research,small-scale trial production,and pilot production.Quality standards for the product were determined,and key parameters were obtained at each stage.The laboratory research demonstrated that the optimal SLPs comprised 15 mL 3%polyvinyl alcohol(PVA)per 1.0 g tilmicosin and 2.5 g carnauba wax(WAX).During small-scale production,modifications were made to the volume of the aqueous phase,emulsifier concentration,and emulsification strength,setting them to 16 mL,5%,and 2200 r/min,respectively.In the pilot production stage,the shear time was considered optimal at eight min.The impurity,content,polydispersion coefficient(PDI),and size of the pilot product were<3%,5%,0385 and 2.64μm,respectively.Among the several parameters studied,heating temperature,drug-lipid ratio,and emulsifier concentration were identified as the main factors affecting product quality,and they were regulated at 100℃,1:3,and 5%,respectively.A novel hot melt emulsification shear method aided the development of a new solid lipid-based suspension from its preliminary stages in the laboratory to pilot production.This innovation is expected to enhance solid lipid-based suspensions'industrial evolution extensively.展开更多
Objective Blood culture remains the gold standard for diagnosing bloodstream infections.Clinical laboratories must ensure the quality of blood culture processes from receipt to obtaining definitive results.We examined...Objective Blood culture remains the gold standard for diagnosing bloodstream infections.Clinical laboratories must ensure the quality of blood culture processes from receipt to obtaining definitive results.We examined laboratory analytical indicators associated with positive blood culture results.Methods Blood cultures collected from Peking Union Medical College Hospital between January 1,2020,and December 31,2022,were retrospectively analyzed.The mode of transportation(piping logistics delivery vs.staff),source of blood cultures(outpatient/emergency department vs.inpatient department),rotation of personnel,and time of reception(8:00–19:59 vs.20:00–07:59)were compared between blood culture-positive and-negative results.Results Between 2020 and 2022,the total positive rate of blood culture was 8.07%.The positive rate of blood cultures in the outpatient/emergency department was significantly higher than that in the inpatient department(12.46%vs.5.83%;P<0.0001).The time-to-detection of blood cultures was significantly affected by the delivery mode and personnel rotation.The blood culture positive rate of the total pre-analytical time within 1 h was significantly higher than that within 1–2 h or>2 h(P<0.0170).Conclusion Laboratory analytical indicators such as patient source,transportation mode,and personnel rotation significantly impacted the positive detection rate or time of blood culture.展开更多
This study describes the design and performance of a laboratory-based tender X-ray spectrometer for X-ray absorption spectroscopy.The system enables effective absorption spectra to be measured within the 2.0-9.0 keV r...This study describes the design and performance of a laboratory-based tender X-ray spectrometer for X-ray absorption spectroscopy.The system enables effective absorption spectra to be measured within the 2.0-9.0 keV range using Rowland circle geometry;it covers the K edge of 3d transition metals,the L edge of lanthanides,and the M edge of actinides.The spectrometer is configured with a Rowland circle with a diameter of 500 mm and integrates a 250 W liquid metal jet X-ray source,spherical bent crystal analyzer,and energy-resolving silicon drift detector.The X-ray source is installed outside the vacuum chamber and remains fixed,while the analyzer crystals and detector are adjusted to change the Bragg angle,maintaining the Rowland condition.The energy resolution is 0.36-1.30 eV at 2.0-9.0 keV,and the monochromatic flux is approximately 5×10^(5) counts/s at 7040 eV.This study highlights the primary characteristics of the spectrometer and demon-strates its capabilities using selected experimental examples.The successful development of this spectrometer can facilitate research on actinide elements,which are often constrained in synchrotron radiation experiments owing to their radioactivity,thus fostering advancements in related nuclear energy fields.展开更多
In this study,the design and development of a sensor made of low-cost parts to monitor inclination and acceleration are presented.Αmicro electro-mechanical systems,micro electro mechanical systems,sensor was housed i...In this study,the design and development of a sensor made of low-cost parts to monitor inclination and acceleration are presented.Αmicro electro-mechanical systems,micro electro mechanical systems,sensor was housed in a robust enclosure and interfaced with a Raspberry Pi microcomputer with Internet connectivity into a proposed tilt and acceleration monitoring node.Online capabilities accessible by mobile phone such as real-time graph,early warning notification,and database logging were implemented using Python programming.The sensor response was calibrated for inherent bias and errors,and then tested thoroughly in the laboratory under static and dynamic loading conditions beside high-quality transducers.Satisfactory accuracy was achieved in real time using the Complementary Filter method,and it was further improved in LabVIEW using Kalman Filters with parameter tuning.A sensor interface with LabVIEW and a 600 MHz CPU microcontroller allowed real-time implementation of highspeed embedded filters,further optimizing sensor results.Kalman and embedded filtering results show agreement for the sensor,followed closely by the lowcomplexity complementary filter applied in real time.The sensor's dynamic response was also verified by shaking table tests,simulating past recorded seismic excitations or artificial vibrations,indicating negligible effect of external acceleration on measured tilt;sensor measurements were benchmarked using highquality tilt and acceleration measuring transducers.A preliminary field evaluation shows robustness of the sensor to harsh weather conditions.展开更多
Natural earthquakes and micro-seismicity resulting from hydraulic fracturing or other engineering practices display distinctively different spatial-temporal features,like mixed burst-and swarm-like features or predomi...Natural earthquakes and micro-seismicity resulting from hydraulic fracturing or other engineering practices display distinctively different spatial-temporal features,like mixed burst-and swarm-like features or predominantly swarm-like features.The mechanism(s)contributing to such observations can be diverse.We present the inspections on the dynamic formation process of the single swarm-like tree in laboratory acoustic emission(AE)catalogs.Such largest swarm-like trees can contain>97%AE events from the entire catalog within a test;and all catalogs under investigation display scale-invariance features.The formation of the largest swarm-like tree correlates with the rock fracture process analogue of the source pervasive process,where its AE releases exhibit significant spatial well-organization.Comparison to other laboratory catalogs under different laboratory settings helps us identify the spatial continuity of the rock fracture process as the primary factor in forming the largest swarm-like trees at laboratory scale.The stress transfer process is involved in the rock fracture process for the tests having pre-existing spatial discontinuity.Artificial perturbations on the spatial information induced by the stress transfer process further confirm that stress transfer also serves to shift the pure swarm-like catalog into a mixed burst-and swarm-like catalog.These laboratory observations may provide inspirational insights for understanding the field-scale mechanism(s)shaping the spatial-temporal energy release features.展开更多
Underground mine pillars provide natural stability to the mine area,allowing safe operations for workers and machinery.Extensive prior research has been conducted to understand pillar failure mechanics and design safe...Underground mine pillars provide natural stability to the mine area,allowing safe operations for workers and machinery.Extensive prior research has been conducted to understand pillar failure mechanics and design safe pillar layouts.However,limited studies(mostly based on empirical field observation and small-scale laboratory tests)have considered pillar-support interactions under monotonic loading conditions for the design of pillar-support systems.This study used a series of large-scale laboratory compression tests on porous limestone blocks to analyze rock and support behavior at a sufficiently large scale(specimens with edge length of 0.5 m)for incorporation of actual support elements,with consideration of different w/h ratios.Both unsupported and supported(grouted rebar rockbolt and wire mesh)tests were conducted,and the surface deformations of the specimens were monitored using three-dimensional(3D)digital image correlation(DIC).Rockbolts instrumented with distributed fiber optic strain sensors were used to study rockbolt strain distribution,load mobilization,and localized deformation at different w/h ratios.Both axial and bending strains were observed in the rockbolts,which became more prominent in the post-peak region of the stress-strain curve.展开更多
Layered rock masses represent complex geological formations commonly encountered in the surrounding rock of deep engineering excavations(Hou et al.,2019;Xu et al.,2017;Yang C H et al.,2009;Xian and Tan,1989).These roc...Layered rock masses represent complex geological formations commonly encountered in the surrounding rock of deep engineering excavations(Hou et al.,2019;Xu et al.,2017;Yang C H et al.,2009;Xian and Tan,1989).These rock masses are predominantly composed of sedimentary,para-metamorphic,and volcanic rock types,characterized by a set of prominent,primary bedding structural planes(layers)exhibiting relatively consistent orientations and significant spatial continuity.展开更多
In recent years,with the continuous deepening of educational reform in the medical field,standardized training,as an important stage of providing excellent laboratory physicians to the clinic,not only needs to strengt...In recent years,with the continuous deepening of educational reform in the medical field,standardized training,as an important stage of providing excellent laboratory physicians to the clinic,not only needs to strengthen various professional skills of laboratory physicians,but also needs to integrate medical ethics education into the education.Report analysis uses clinical case test reports as teaching content.Under the guidance of attending physicians,resident physicians are the main body of standardized training.Teaching activities for report analysis are carried out by combining theoretical knowledge and case information.Through detailed planning of report analysis teaching activities and adequate preparation,standardized training activities are smoothly carried out.This targeted and comprehensive teaching model can effectively improve the clinical thinking and clinical laboratory professional ability of resident physicians,strengthen medical ethics,and make biochemistry sub-specialty resident physicians in laboratory medicine more adaptable to clinical work,thus achieving teaching goals.展开更多
For non-English major college students,the listening section of the College English Test Band 4(CET-4)is both a critical and challenging component of exam preparation.Many students struggle with various difficulties d...For non-English major college students,the listening section of the College English Test Band 4(CET-4)is both a critical and challenging component of exam preparation.Many students struggle with various difficulties during their listening practice.To address these challenges,open intelligent laboratories equipped with digital technology provide effective solutions.These laboratories offer a diverse range of listening resources,exposing students to various accents and topics,which helps them adapt to different listening styles and improve comprehension.Additionally,they provide simulated tests and interactive exercises that replicate exam conditions,allowing students to practice time management and develop effective test-taking strategies.Furthermore,artificial intelligence algorithms integrated into these labs analyze students’strengths and weaknesses,offering personalized feedback and targeted recommendations for improvement.By leveraging smart technology in education,intelligent laboratories can significantly enhance students’listening skills and CET-4 performance,facilitating more effective learning and advancing educational practices.This paper examines the primary challenges students face in CET-4 listening preparation and explores how digital tools in open intelligent laboratories can be utilized to develop effective preparation strategies.展开更多
Since the early days of assisted reproductive technology(ART),the importance of sperm processing,employed to separate the motile,morphologically normal sperm from the semen,has been shown to be beneficial.The aim of t...Since the early days of assisted reproductive technology(ART),the importance of sperm processing,employed to separate the motile,morphologically normal sperm from the semen,has been shown to be beneficial.The aim of the semen processing technique has been to remove seminal plasma and facilitate capacitation.Additionally,the presence of leukocytes,bacteria,and dead spermatozoa has been shown to be detrimental as it may cause oxidative stress that has an adverse effect on oocyte fertilization and embryo development.Hence,removal of leukocytes,bacteria,and dead spermatozoa is an important step of sperm processing for assisted reproduction.Currently,several sperm processing techniques have been evolved and optimized in the field of assisted reproduction.The requirements for in vitro fertilization(IVF),intracytoplasmic sperm injection(ICSI),and testicular sperm extraction(TESE)are different than those of intrauterine insemination(IUI).The yield of as many motile,morphologically normal sperm as possible is a prerequisite for the success of IVF insemination procedure.In ICSI,where injection of a single spermatozoon into the oocyte is performed by the embryologist,sperm selection techniques play a crucial role in the ICSI procedure.Finally,sperm retrieval in TESE samples with very low number of sperm may be challenging and requires extra care during sample processing.Additionally,sperm cryopreservation is necessary in TESE cases in order to avoid multiple biopsies.展开更多
Remembrance activities can support the Culture of Care(CoC)in Laboratory Animal Science(LAS)not only by promoting a culture of respect,gratitude and thankfulness for animal life but also by helping the emotional proce...Remembrance activities can support the Culture of Care(CoC)in Laboratory Animal Science(LAS)not only by promoting a culture of respect,gratitude and thankfulness for animal life but also by helping the emotional processing and healing of lab animal researchers and animal facility staff.Even though remembrance activities are practiced in many parts of the world,we did not come across any reported cases in Sri Lanka before 2022.Therefore,here,we report on the various remembrance activities and practices observed within our local scientific community.展开更多
To build a community of scientific innovation,serve the country’s goals of carbon peak and carbon neutrality,promote application of scientific research outcomes in the Guangdong-Hong Kong-Macao Greater Bay Area(GBA),...To build a community of scientific innovation,serve the country’s goals of carbon peak and carbon neutrality,promote application of scientific research outcomes in the Guangdong-Hong Kong-Macao Greater Bay Area(GBA),and accelerate the formation of new quality productive forces in the green energy industry,the Yangjiang Offshore Wind Power Laboratory and the GBA Institute of Industry and Talent(GBAIIT)recently signed a strategic cooperation agreement.展开更多
Medical laboratory science is an essential component of healthcare services.The quality of laboratory services is not only highly correlated with doctors’theoretical knowledge and practical skills but is also greatly...Medical laboratory science is an essential component of healthcare services.The quality of laboratory services is not only highly correlated with doctors’theoretical knowledge and practical skills but is also greatly influenced by their professional ethics and ideological standards.Therefore,it is necessary to implement ideological and political education in the curriculum for medical laboratory students,aiming to elevate their ideological and moral standards,enhance their work enthusiasm,and alleviate job burnout.This article explores the positive impact of ideological and political education on cultivating professionalism in medical laboratory students and discusses its application in this context,considering the current situation of professional ethics training for these students.展开更多
文摘State Key Laboratory of Baiyun Obo Rare Earth Resource Researches and Comprehensive Utilization was approved by the Ministry of Science and Technology to be one of the national key laboratories in November 2022.
基金supported by Public Health Talent Training and Surport Plan(National Administration of Disease Prevention and Control)Research and application of new technology for rapid monitoring and tracing of emergent infectious diseases among entry-exit population(2024YFFK0056)Monitoring,Early warning and Response of Major Infectious Diseases(2022ZDZX0017).
文摘Objective This study reports the first imported case of Lassa fever(LF)in China.Laboratory detection and molecular epidemiological analysis of the Lassa virus(LASV)from this case offer valuable insights for the prevention and control of LF.Methods Samples of cerebrospinal fluid(CSF),blood,urine,saliva,and environmental materials were collected from the patient and their close contacts for LASV nucleotide detection.Whole-genome sequencing was performed on positive samples to analyze the genetic characteristics of the virus.Results LASV was detected in the patient’s CSF,blood,and urine,while all samples from close contacts and the environment tested negative.The virus belongs to the lineage IV strain and shares the highest homology with strains from Sierra Leone.The variability in the glycoprotein complex(GPC)among different strains ranged from 3.9%to 15.1%,higher than previously reported for the seven known lineages.Amino acid mutation analysis revealed multiple mutations within the GPC immunogenic epitopes,increasing strain diversity and potentially impacting immune response.Conclusion The case was confirmed through nucleotide detection,with no evidence of secondary transmission or viral spread.The LASV strain identified belongs to lineage IV,with broader GPC variability than previously reported.Mutations in the immune-related sites of GPC may affect immune responses,necessitating heightened vigilance regarding the virus.
文摘State Key Laboratory of Baiyun Obo Rare Earth Resource Researches and Comprehensive Utilization was approved by the Ministry of Science and Technology to be one of the national key laboratories in November 2022.The laboratory was reconstructed based on former State Key Laboratory of Baiyun Obo Rare Earth Resources Researches and Comprehensive Utilization.
文摘Background:Medical laboratory diagnostic services play a critical role in the diagnosis,treatment,and manage-ment of diseases,forming the cornerstone of effective healthcare systems.Despite the crucial role of laboratory services,the quality and accessibility of medical laboratory services in sub-Saharan Africa(SSA)face signifi-cant challenges.Global health development aid has been pivotal in supporting SSA laboratory services.This study aimed to examine global health development aid initiatives that have successfully enhanced the quality of medical laboratory services and the challenges and barriers to effectively improving medical laboratory services through global health development aid in SSA.Methods:We used a narrative review study design.We searched PubMed,Web of Science and Scopus for articles published in the last 15 years.These three databases are generally considered premier databases for peer-reviewed articles in global health,public health,health systems,and biomedical sciences.The inclusion criteria for this review included research studies,reports,and grey literature.Only articles published in English from 2010 on-ward were considered.The analysis followed a qualitative approach,emphasizing thematic synthesis and critical interpretation.Results:Forty articles were included in this study.Of these,18 were primary research studies,11 were reports,7 were commentaries,and 4 were reviews.Five sub-themes from the successful global health development aid initiative themes were capacity building and training programs,infrastructure development,partnership models,policy advocacy and regulatory support,quality control and standardization of laboratory services.The sub-themes from the challenges and barrier theme were insufficient funding and resource allocation,human resource constraints,inadequate infrastructure and equipment,and political and institutional barriers.This review re-vealed that several factors,including financial sustainability,human resource capacity,institutional support,resilience,and effective monitoring systems,shape the sustainability of improvements in medical laboratory services in SSA.Conclusion:Achieving long-term sustainability requires strategies that ensure financial self-sufficiency,foster a skilled and stable workforce,and integrate laboratory services into national health frameworks.
基金supported by the National Key Research and Development Program of China(Grant No.2023YFB2390300)the National Natural Science Foundation of China(Grant No.42172292)Yinlin Ji is grateful for the support by the Helmholtz Association's Initiative and Networking Fund for the Helmholtz Young Investigator Group ARES(Contract No.VH-NG-1516).
文摘This study aims to understand the effect of injection rate on injection-induced fracture activation in granite.We performed water injection-induced slip tests on samples containing either a smooth or a rough fracture at four different injection rates under undrained conditions and monitored the acoustic emission(AE)signals during the tests.Experimental results reveal that the critical activation fluid pressure is related to the injection rate,pressure diffusion rate,stress state,and fracture roughness.For the smooth fracture,as the injection rate increases,the critical activation fluid pressure increases significantly,while the injection rate has little effect on the critical activation fluid pressure of the rough fracture.The quasi-static slip distance of fractures decreases as the injection rate increases,with rough fractures exhibiting a greater overall slip distance compared to smooth fractures.The number of AE events per unit sliding distance increases with the injection rate,while the global b value decreases.These results indicate that higher injection rates produce more large-magnitude AE events and more severe slip instability and asperity damage.We established a linkage between fluid injection volume,injection rate,and AE events using the seismogenic index(Σ).The smooth fracture exhibits a steadily increasingΣwith the elapse of injection time,and the rate of increase is higher at higher injection rates;while the rough fracture is featured by a fluctuatingΣ,signifying the intermittent occurrence of large-magnitude AE events associated with the damage of larger fracture asperities.Our results highlight the importance of fracture surface heterogeneity on injection-induced fracture activation and slip.
文摘With the continuous advancement of the tiered diagnosis and treatment system,the medical consortium model has gained increasing attention as an important approach to promoting the vertical integration of healthcare resources.Within this context,laboratory data,as a key component of healthcare information systems,urgently requires efficient sharing and intelligent analysis.This paper designs and constructs an intelligent early warning system for laboratory data based on a cloud platform tailored to the medical consortium model.Through standardized data formats and unified access interfaces,the system enables the integration and cleaning of laboratory data across multiple healthcare institutions.By combining medical rule sets with machine learning models,the system achieves graded alerts and rapid responses to abnormal key indicators and potential outbreaks of infectious diseases.Practical deployment results demonstrate that the system significantly improves the utilization efficiency of laboratory data,strengthens public health event monitoring,and optimizes inter-institutional collaboration.The paper also discusses challenges encountered during system implementation,such as inconsistent data standards,security and compliance concerns,and model interpretability,and proposes corresponding optimization strategies.These findings provide a reference for the broader application of intelligent medical early warning systems.
文摘Against the backdrop of the rapid intelligent development of medical laboratory technology,vocational education is in urgent need of teaching innovation.This paper explores the integration and practice of information-based teaching methods in vocational education of medical laboratory technology,analyzes the current situation of traditional teaching and the demand for informatization,and elaborates on the practical paths from aspects such as resource construction,model innovation,and evaluation reform.Practice has verified that this integration has improved teaching quality and students’skills,but there are problems such as uneven resources and the need to improve teachers’and students’abilities.Countermeasures such as strengthening co-construction and sharing of resources and carrying out special training are proposed to provide reference for the development of professional education.
基金supported by the Fundamental Research Funds for the Central Universities(2662020DKPY008)the National Natural Science Foundation of China(grant No.31772797)。
文摘The clinical application of solid lipid particles(SLPs)is hampered due to the need for advanced nano/micro-suspension production technology.This research aims to establish a pilot-scale production line employing high-speed shears as emulsification equipment.The primary purpose is to manufacture nano/micro-suspensions using solid lipid particles(SLPs).The study also exhaustively introduces and analyzes the regulatory schemes for process parameters and formulations at various stages of production.The process and formulation endured optimization through orthog-onal or single-factor tests at various production steps:laboratory research,small-scale trial production,and pilot production.Quality standards for the product were determined,and key parameters were obtained at each stage.The laboratory research demonstrated that the optimal SLPs comprised 15 mL 3%polyvinyl alcohol(PVA)per 1.0 g tilmicosin and 2.5 g carnauba wax(WAX).During small-scale production,modifications were made to the volume of the aqueous phase,emulsifier concentration,and emulsification strength,setting them to 16 mL,5%,and 2200 r/min,respectively.In the pilot production stage,the shear time was considered optimal at eight min.The impurity,content,polydispersion coefficient(PDI),and size of the pilot product were<3%,5%,0385 and 2.64μm,respectively.Among the several parameters studied,heating temperature,drug-lipid ratio,and emulsifier concentration were identified as the main factors affecting product quality,and they were regulated at 100℃,1:3,and 5%,respectively.A novel hot melt emulsification shear method aided the development of a new solid lipid-based suspension from its preliminary stages in the laboratory to pilot production.This innovation is expected to enhance solid lipid-based suspensions'industrial evolution extensively.
基金supported by grants from the National High Level Hospital Clinical Research Funding(2022-PUMCH-B-074)Peking Union Medical College Hospital Research Funding for Postdoc(kyfyjj202320).
文摘Objective Blood culture remains the gold standard for diagnosing bloodstream infections.Clinical laboratories must ensure the quality of blood culture processes from receipt to obtaining definitive results.We examined laboratory analytical indicators associated with positive blood culture results.Methods Blood cultures collected from Peking Union Medical College Hospital between January 1,2020,and December 31,2022,were retrospectively analyzed.The mode of transportation(piping logistics delivery vs.staff),source of blood cultures(outpatient/emergency department vs.inpatient department),rotation of personnel,and time of reception(8:00–19:59 vs.20:00–07:59)were compared between blood culture-positive and-negative results.Results Between 2020 and 2022,the total positive rate of blood culture was 8.07%.The positive rate of blood cultures in the outpatient/emergency department was significantly higher than that in the inpatient department(12.46%vs.5.83%;P<0.0001).The time-to-detection of blood cultures was significantly affected by the delivery mode and personnel rotation.The blood culture positive rate of the total pre-analytical time within 1 h was significantly higher than that within 1–2 h or>2 h(P<0.0170).Conclusion Laboratory analytical indicators such as patient source,transportation mode,and personnel rotation significantly impacted the positive detection rate or time of blood culture.
基金supported by the Instrument and Equipment Development Program of the Chinese Academy of Science(Grant No.YJKYYQ20180066)the National Natural Science Foundation of China(No.22227809)+2 种基金the Shanghai Science and Technology Innovation Action Plan(No.22142200300)the Science and Technology Talents Program of the Shanghai Institute of Applied Physics(No.SINAP-KJZX-202204)the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA 0400000).
文摘This study describes the design and performance of a laboratory-based tender X-ray spectrometer for X-ray absorption spectroscopy.The system enables effective absorption spectra to be measured within the 2.0-9.0 keV range using Rowland circle geometry;it covers the K edge of 3d transition metals,the L edge of lanthanides,and the M edge of actinides.The spectrometer is configured with a Rowland circle with a diameter of 500 mm and integrates a 250 W liquid metal jet X-ray source,spherical bent crystal analyzer,and energy-resolving silicon drift detector.The X-ray source is installed outside the vacuum chamber and remains fixed,while the analyzer crystals and detector are adjusted to change the Bragg angle,maintaining the Rowland condition.The energy resolution is 0.36-1.30 eV at 2.0-9.0 keV,and the monochromatic flux is approximately 5×10^(5) counts/s at 7040 eV.This study highlights the primary characteristics of the spectrometer and demon-strates its capabilities using selected experimental examples.The successful development of this spectrometer can facilitate research on actinide elements,which are often constrained in synchrotron radiation experiments owing to their radioactivity,thus fostering advancements in related nuclear energy fields.
基金Research Committee,National Technical University of Athens。
文摘In this study,the design and development of a sensor made of low-cost parts to monitor inclination and acceleration are presented.Αmicro electro-mechanical systems,micro electro mechanical systems,sensor was housed in a robust enclosure and interfaced with a Raspberry Pi microcomputer with Internet connectivity into a proposed tilt and acceleration monitoring node.Online capabilities accessible by mobile phone such as real-time graph,early warning notification,and database logging were implemented using Python programming.The sensor response was calibrated for inherent bias and errors,and then tested thoroughly in the laboratory under static and dynamic loading conditions beside high-quality transducers.Satisfactory accuracy was achieved in real time using the Complementary Filter method,and it was further improved in LabVIEW using Kalman Filters with parameter tuning.A sensor interface with LabVIEW and a 600 MHz CPU microcontroller allowed real-time implementation of highspeed embedded filters,further optimizing sensor results.Kalman and embedded filtering results show agreement for the sensor,followed closely by the lowcomplexity complementary filter applied in real time.The sensor's dynamic response was also verified by shaking table tests,simulating past recorded seismic excitations or artificial vibrations,indicating negligible effect of external acceleration on measured tilt;sensor measurements were benchmarked using highquality tilt and acceleration measuring transducers.A preliminary field evaluation shows robustness of the sensor to harsh weather conditions.
文摘Natural earthquakes and micro-seismicity resulting from hydraulic fracturing or other engineering practices display distinctively different spatial-temporal features,like mixed burst-and swarm-like features or predominantly swarm-like features.The mechanism(s)contributing to such observations can be diverse.We present the inspections on the dynamic formation process of the single swarm-like tree in laboratory acoustic emission(AE)catalogs.Such largest swarm-like trees can contain>97%AE events from the entire catalog within a test;and all catalogs under investigation display scale-invariance features.The formation of the largest swarm-like tree correlates with the rock fracture process analogue of the source pervasive process,where its AE releases exhibit significant spatial well-organization.Comparison to other laboratory catalogs under different laboratory settings helps us identify the spatial continuity of the rock fracture process as the primary factor in forming the largest swarm-like trees at laboratory scale.The stress transfer process is involved in the rock fracture process for the tests having pre-existing spatial discontinuity.Artificial perturbations on the spatial information induced by the stress transfer process further confirm that stress transfer also serves to shift the pure swarm-like catalog into a mixed burst-and swarm-like catalog.These laboratory observations may provide inspirational insights for understanding the field-scale mechanism(s)shaping the spatial-temporal energy release features.
基金the funding support from Alpha Foundation for the Improvement of Mine Safety and Health Inc.(ALPHAFOUNDATION,Grant No.AFC820-52)。
文摘Underground mine pillars provide natural stability to the mine area,allowing safe operations for workers and machinery.Extensive prior research has been conducted to understand pillar failure mechanics and design safe pillar layouts.However,limited studies(mostly based on empirical field observation and small-scale laboratory tests)have considered pillar-support interactions under monotonic loading conditions for the design of pillar-support systems.This study used a series of large-scale laboratory compression tests on porous limestone blocks to analyze rock and support behavior at a sufficiently large scale(specimens with edge length of 0.5 m)for incorporation of actual support elements,with consideration of different w/h ratios.Both unsupported and supported(grouted rebar rockbolt and wire mesh)tests were conducted,and the surface deformations of the specimens were monitored using three-dimensional(3D)digital image correlation(DIC).Rockbolts instrumented with distributed fiber optic strain sensors were used to study rockbolt strain distribution,load mobilization,and localized deformation at different w/h ratios.Both axial and bending strains were observed in the rockbolts,which became more prominent in the post-peak region of the stress-strain curve.
基金supported by the National Natural Science Foundation of China(Nos.42107211 and U23A20651)the Natural Science Foundation of Sichuan Province(No.2025ZNSFSC0097)。
文摘Layered rock masses represent complex geological formations commonly encountered in the surrounding rock of deep engineering excavations(Hou et al.,2019;Xu et al.,2017;Yang C H et al.,2009;Xian and Tan,1989).These rock masses are predominantly composed of sedimentary,para-metamorphic,and volcanic rock types,characterized by a set of prominent,primary bedding structural planes(layers)exhibiting relatively consistent orientations and significant spatial continuity.
文摘In recent years,with the continuous deepening of educational reform in the medical field,standardized training,as an important stage of providing excellent laboratory physicians to the clinic,not only needs to strengthen various professional skills of laboratory physicians,but also needs to integrate medical ethics education into the education.Report analysis uses clinical case test reports as teaching content.Under the guidance of attending physicians,resident physicians are the main body of standardized training.Teaching activities for report analysis are carried out by combining theoretical knowledge and case information.Through detailed planning of report analysis teaching activities and adequate preparation,standardized training activities are smoothly carried out.This targeted and comprehensive teaching model can effectively improve the clinical thinking and clinical laboratory professional ability of resident physicians,strengthen medical ethics,and make biochemistry sub-specialty resident physicians in laboratory medicine more adaptable to clinical work,thus achieving teaching goals.
文摘For non-English major college students,the listening section of the College English Test Band 4(CET-4)is both a critical and challenging component of exam preparation.Many students struggle with various difficulties during their listening practice.To address these challenges,open intelligent laboratories equipped with digital technology provide effective solutions.These laboratories offer a diverse range of listening resources,exposing students to various accents and topics,which helps them adapt to different listening styles and improve comprehension.Additionally,they provide simulated tests and interactive exercises that replicate exam conditions,allowing students to practice time management and develop effective test-taking strategies.Furthermore,artificial intelligence algorithms integrated into these labs analyze students’strengths and weaknesses,offering personalized feedback and targeted recommendations for improvement.By leveraging smart technology in education,intelligent laboratories can significantly enhance students’listening skills and CET-4 performance,facilitating more effective learning and advancing educational practices.This paper examines the primary challenges students face in CET-4 listening preparation and explores how digital tools in open intelligent laboratories can be utilized to develop effective preparation strategies.
文摘Since the early days of assisted reproductive technology(ART),the importance of sperm processing,employed to separate the motile,morphologically normal sperm from the semen,has been shown to be beneficial.The aim of the semen processing technique has been to remove seminal plasma and facilitate capacitation.Additionally,the presence of leukocytes,bacteria,and dead spermatozoa has been shown to be detrimental as it may cause oxidative stress that has an adverse effect on oocyte fertilization and embryo development.Hence,removal of leukocytes,bacteria,and dead spermatozoa is an important step of sperm processing for assisted reproduction.Currently,several sperm processing techniques have been evolved and optimized in the field of assisted reproduction.The requirements for in vitro fertilization(IVF),intracytoplasmic sperm injection(ICSI),and testicular sperm extraction(TESE)are different than those of intrauterine insemination(IUI).The yield of as many motile,morphologically normal sperm as possible is a prerequisite for the success of IVF insemination procedure.In ICSI,where injection of a single spermatozoon into the oocyte is performed by the embryologist,sperm selection techniques play a crucial role in the ICSI procedure.Finally,sperm retrieval in TESE samples with very low number of sperm may be challenging and requires extra care during sample processing.Additionally,sperm cryopreservation is necessary in TESE cases in order to avoid multiple biopsies.
文摘Remembrance activities can support the Culture of Care(CoC)in Laboratory Animal Science(LAS)not only by promoting a culture of respect,gratitude and thankfulness for animal life but also by helping the emotional processing and healing of lab animal researchers and animal facility staff.Even though remembrance activities are practiced in many parts of the world,we did not come across any reported cases in Sri Lanka before 2022.Therefore,here,we report on the various remembrance activities and practices observed within our local scientific community.
文摘To build a community of scientific innovation,serve the country’s goals of carbon peak and carbon neutrality,promote application of scientific research outcomes in the Guangdong-Hong Kong-Macao Greater Bay Area(GBA),and accelerate the formation of new quality productive forces in the green energy industry,the Yangjiang Offshore Wind Power Laboratory and the GBA Institute of Industry and Talent(GBAIIT)recently signed a strategic cooperation agreement.
文摘Medical laboratory science is an essential component of healthcare services.The quality of laboratory services is not only highly correlated with doctors’theoretical knowledge and practical skills but is also greatly influenced by their professional ethics and ideological standards.Therefore,it is necessary to implement ideological and political education in the curriculum for medical laboratory students,aiming to elevate their ideological and moral standards,enhance their work enthusiasm,and alleviate job burnout.This article explores the positive impact of ideological and political education on cultivating professionalism in medical laboratory students and discusses its application in this context,considering the current situation of professional ethics training for these students.
