目的:观察siRNA沉默核糖体蛋白L31(ribosomal protein L31,RPL31)基因表达对人胰腺癌PANC-1细胞的影响.方法:针对RPL31基因设计了3条siRNA,使用脂质体LipofectamineTM2000转染人胰腺癌PANC-1细胞,同时设立空白对照组和阴性对照组,转染...目的:观察siRNA沉默核糖体蛋白L31(ribosomal protein L31,RPL31)基因表达对人胰腺癌PANC-1细胞的影响.方法:针对RPL31基因设计了3条siRNA,使用脂质体LipofectamineTM2000转染人胰腺癌PANC-1细胞,同时设立空白对照组和阴性对照组,转染成功后通过实时定量PCR及Western blot分别从mRNA和蛋白水平检测RNA干扰沉默RPL31基因的效果,四氮唑盐比色法(MTT)检测细胞增殖,流式细胞术检测细胞周期分布,Transwell小室检测细胞迁移,ELISA检测血管内皮生长因子(vascular endothelial growth factor,VEGF)的表达.结果:转染48h后,3条siRNA能显著抑制RPL31基因mRNA和蛋白的表达;MTT结果显示,PANC-1细胞增殖明显受到抑制;细胞周期分布中G0/G1期明显增加、S期减少(G0/G1:59.85%±5.47%vs45.71%±3.44%;S:28.63%±4.52%vs45.13%±2.64%,均P<0.05);转染后细胞迁移数目(178.6个±30.3个)较空白对照组(470.5个±22.8个)与阴性对照组(474.2个±20.4个)明显减少,差异显著(P<0.05);转染后PANC-1细胞的VEGF表达量也明显减少[1563.45pg/(106cell24h)±24.95pg/(106cell24h)vs2804.6pg/(106cell24h)±40.46pg/(106cell24h),2791.5pg/(106cell24h)±44.77pg/(106cell24h)].结论:RPL31siRNA能明显抑制PANC-1细胞RPL31的表达,抑制细胞增殖,降低细胞迁移及VEGF表达,RPL31基因有可能成为胰腺癌基因治疗的新靶点.展开更多
目的:研究siRNA沉默糖体蛋白L31(ribosomal protein L31,RPL31)对人胰腺癌BxPC-3细胞裸鼠皮下移植瘤的抑制作用,探讨RPL31基因在胰腺癌中可能的作用机制.方法:设计合成靶向人RPL31基因的siRNA及阴性对照siRNA;建立人胰腺癌BxPC-3细胞的B...目的:研究siRNA沉默糖体蛋白L31(ribosomal protein L31,RPL31)对人胰腺癌BxPC-3细胞裸鼠皮下移植瘤的抑制作用,探讨RPL31基因在胰腺癌中可能的作用机制.方法:设计合成靶向人RPL31基因的siRNA及阴性对照siRNA;建立人胰腺癌BxPC-3细胞的Balb/c裸鼠皮下移植瘤模型,按照移植瘤体积随机化分为3组:溶剂对照组、阴性对照组及RPL31-siRNA干预组;使用RNAi-Mate转染试剂将RPL31-siRNA进行移植瘤瘤内注射,观察各组裸鼠体内瘤体生长速度,绘制肿瘤生长曲线;采用实时定量PCR及Western blot的方法检测移植瘤组织RPL31基因的表达;免疫组织化学法(immunohistochemistry,IHC)检测裸鼠皮下移植瘤Ki-67及CD31的表达;原位末端标记技术(TUNEL)检测移植瘤组织的细胞凋亡.结果:与溶剂对照组和阴性对照组相比,RPL31-siRNA注射组裸鼠皮下移植瘤生长缓慢,移植瘤体积明显缩小,差异具有统计学意义(P<0.05);移植瘤组织中RPL31基因的mRNA水平和蛋白水平表达下调;另外,RPL31-siRNA注射组裸鼠移植瘤中Ki-67表达下调(55.78%±4.63%、51.37%±5.05%vs11.08%±1.31%),CD31的表达下调(56.53%±6.03%、44.84%±5.24%vs9.67%±1.39%);RPL31-siRNA注射组裸鼠移植瘤细胞凋亡增加(2.92%±0.54%、3.85%±0.87%vs39.58%±4.02%).结论:RPL31-siRNA可以下调胰腺癌BxPC-3细胞裸鼠皮下移植瘤中RPL31基因的表达,抑制移植瘤的生长,并且还可以抑制移植瘤中Ki-67及CD31的表达,诱导移植瘤细胞的凋亡.以RPL31为靶点的基因治疗有潜在临床应用前景.展开更多
The abilities of typhoon (TC) track prediction by a medium-range forecast model T213L31 at National Meteorological Center are analyzed and its ability to improve its TC forecasts is discussed. The results show that ab...The abilities of typhoon (TC) track prediction by a medium-range forecast model T213L31 at National Meteorological Center are analyzed and its ability to improve its TC forecasts is discussed. The results show that about 57% of the TCs could be predicted by T213L31 but the initial position errors are large. The 43% area without the prediction of TC tracks is concentrated between 13°N and 20°N and east of 120°E and lack of conventional observation data is the main reason for the absence of TC prediction in this area. The adding of bogus TC could improve the ability of TC track prediction when there is no TC vortex in the analysis field, but could only have positive effects on the short-range TC track prediction when there is TC vortex in the T213L31 analysis field.展开更多
文摘目的:研究siRNA沉默糖体蛋白L31(ribosomal protein L31,RPL31)对人胰腺癌BxPC-3细胞裸鼠皮下移植瘤的抑制作用,探讨RPL31基因在胰腺癌中可能的作用机制.方法:设计合成靶向人RPL31基因的siRNA及阴性对照siRNA;建立人胰腺癌BxPC-3细胞的Balb/c裸鼠皮下移植瘤模型,按照移植瘤体积随机化分为3组:溶剂对照组、阴性对照组及RPL31-siRNA干预组;使用RNAi-Mate转染试剂将RPL31-siRNA进行移植瘤瘤内注射,观察各组裸鼠体内瘤体生长速度,绘制肿瘤生长曲线;采用实时定量PCR及Western blot的方法检测移植瘤组织RPL31基因的表达;免疫组织化学法(immunohistochemistry,IHC)检测裸鼠皮下移植瘤Ki-67及CD31的表达;原位末端标记技术(TUNEL)检测移植瘤组织的细胞凋亡.结果:与溶剂对照组和阴性对照组相比,RPL31-siRNA注射组裸鼠皮下移植瘤生长缓慢,移植瘤体积明显缩小,差异具有统计学意义(P<0.05);移植瘤组织中RPL31基因的mRNA水平和蛋白水平表达下调;另外,RPL31-siRNA注射组裸鼠移植瘤中Ki-67表达下调(55.78%±4.63%、51.37%±5.05%vs11.08%±1.31%),CD31的表达下调(56.53%±6.03%、44.84%±5.24%vs9.67%±1.39%);RPL31-siRNA注射组裸鼠移植瘤细胞凋亡增加(2.92%±0.54%、3.85%±0.87%vs39.58%±4.02%).结论:RPL31-siRNA可以下调胰腺癌BxPC-3细胞裸鼠皮下移植瘤中RPL31基因的表达,抑制移植瘤的生长,并且还可以抑制移植瘤中Ki-67及CD31的表达,诱导移植瘤细胞的凋亡.以RPL31为靶点的基因治疗有潜在临床应用前景.
文摘The abilities of typhoon (TC) track prediction by a medium-range forecast model T213L31 at National Meteorological Center are analyzed and its ability to improve its TC forecasts is discussed. The results show that about 57% of the TCs could be predicted by T213L31 but the initial position errors are large. The 43% area without the prediction of TC tracks is concentrated between 13°N and 20°N and east of 120°E and lack of conventional observation data is the main reason for the absence of TC prediction in this area. The adding of bogus TC could improve the ability of TC track prediction when there is no TC vortex in the analysis field, but could only have positive effects on the short-range TC track prediction when there is TC vortex in the T213L31 analysis field.
