目的 为了开发我国珍稀动物——吉林双阳梅花鹿的基因资源 ,在分子水平上揭示鹿药材的药理作用。方法 构建了梅花鹿脾脏细胞 c DNA质粒文库 ,克隆了一些看家基因并进行了序列分析。结果 其中的一个序列在核酸及推导的氨基酸序列水平...目的 为了开发我国珍稀动物——吉林双阳梅花鹿的基因资源 ,在分子水平上揭示鹿药材的药理作用。方法 构建了梅花鹿脾脏细胞 c DNA质粒文库 ,克隆了一些看家基因并进行了序列分析。结果 其中的一个序列在核酸及推导的氨基酸序列水平上与编码人、犬、大鼠、小鼠核蛋白体蛋白 L 2 7(ribosom al protein L 2 7) c DNA序列和对应的氨基酸序列高度同源 ,并具有完整的开放阅读框架 (ORF)。结论 发现了双阳梅花鹿核蛋白体蛋白 L2 7全长 c DNA序列 ,此序列已经在 Genbank中登录 ,登录号为 :AF3732 31。展开更多
目的:研究益气活血中药复方对CVB3大鼠心肌细胞感染模型核糖体蛋白L27a(ribosomal protein L27a)表达的作用机制,进一步证实益气活血中药复方是治疗CVB3病毒性心肌炎的有效方剂。方法:本实验用新生2～3天大鼠心肌细胞建立柯萨奇病毒B3(C...目的:研究益气活血中药复方对CVB3大鼠心肌细胞感染模型核糖体蛋白L27a(ribosomal protein L27a)表达的作用机制,进一步证实益气活血中药复方是治疗CVB3病毒性心肌炎的有效方剂。方法:本实验用新生2～3天大鼠心肌细胞建立柯萨奇病毒B3(CVB3)感染模型,通过改良的抑制性消减杂交技术(SSH),克隆了受CVB3攻击的宿主细胞中被中药(益气活血中药复方)调控的基因。结果:核糖体蛋白L27a在中药组中呈高表达,病毒组中表达减弱或被抑制。结论:益气活血中药复方能够影响受CVB3攻击的宿主细胞核糖体蛋白L27a的表达,有效保护心肌、阻断病程进度,实现治疗病毒性心肌炎的目的。展开更多
The DNA replication stress(RS)response is crucial for maintaining cellular homeostasis and promoting physiological longevity.However,the mechanisms by which long-lived species,such as bats,regulate RS to maintain geno...The DNA replication stress(RS)response is crucial for maintaining cellular homeostasis and promoting physiological longevity.However,the mechanisms by which long-lived species,such as bats,regulate RS to maintain genomic stability remain unclear.Also,recent studies have uncovered noncanonical roles of ribosome-associated factors in maintaining genomic stability.In this study,somatic skin fibroblasts from the long-lived big-footed bat(Myotis pilosus)were examined,with results showing that bat cells exhibited enhanced RS tolerance compared to mouse cells.Comparative transcriptome analysis under RS conditions revealed pronounced species-specific transcriptional differences,including robust up-regulation of ribosome biogenesis genes in bat cells and a markedly reduced activation of the P53 signaling pathway.These features emphasize a distinct homeostatic strategy in bat cells.Nuclear fragile X mental retardation-interacting protein 1(Nufip1),a ribosome-associated factor highly expressed in bat fibroblasts,was identified as a potential integrator of ribosomal and P53 signaling via its association with ribosomal protein S27-like(Rps27l).These findings provide direct cellular and molecular evidence for a noncanonical RS response in bats,highlighting a deeper understanding of the biological characteristics and genomic maintenance mechanisms of long-lived species.展开更多
Different Ziegler-Natta catalysts were employed to polymerize ethylene. To investigate the influences of reaction parameters, namely Al/Ti molar ratio, hydrogen and processing parameters, i.e. ethylene pressure and te...Different Ziegler-Natta catalysts were employed to polymerize ethylene. To investigate the influences of reaction parameters, namely Al/Ti molar ratio, hydrogen and processing parameters, i.e. ethylene pressure and temperature, a Taguchi experimental design was worked out. An L27 orthogonal array was chosen to take the above-mentioned parameters and relevant interactions into account. Response surface method was the tool used to analyze the experimental design results. Al/Ti, ethylene pressure and temperature were selected as experimental design factors, and catalyst activity and polymerization yield were the response parameters. Increasing pressure, due to an increment in monomer accessibility, and rising Al/Ti, because of higher reduction in the catalysts, cause an increase in both polymerization yield and catalyst activity. Nonetheless, a higher temperature, thanks to reducing ethylene solubility in the slurry medium and partially catalyst destruction, lead to a reduction in both response parameters. A synergistic effect was also observed between temperature and pressure. All catalyst activities will reduce in the presence of hydrogen. Molecular weight also shows a decline in the presence of hydrogen as a transfer agent. However, the polydispersity index remains approximately intact. Using SEM, various morphologies, owing to different catalyst morphologies, were seen for the polyethylene.展开更多
In recent times, lithium-ion batteries have been widely used owing to their high energy density, extended cycle lifespan, and minimal self-discharge rate. The design of high-speed rechargeable lithium-ion batteries fa...In recent times, lithium-ion batteries have been widely used owing to their high energy density, extended cycle lifespan, and minimal self-discharge rate. The design of high-speed rechargeable lithium-ion batteries faces a significant challenge owing to the need to increase average electric power during charging. This challenge results from the direct influence of the power level on the rate of chemical reactions occurring in the battery electrodes. In this study, the Taguchi optimization method was used to enhance the average electric power during the charging process of lithium-ion batteries. The Taguchi technique is a statistical strategy that facilitates the systematic and efficient evaluation of numerous experimental variables. The proposed method involved varying seven input factors, including positive electrode thickness, positive electrode material, positive electrode active material volume fraction, negative electrode active material volume fraction, separator thickness, positive current collector thickness, and negative current collector thickness. Three levels were assigned to each control factor to identify the optimal conditions and maximize the average electric power during charging. Moreover, a variance assessment analysis was conducted to validate the results obtained from the Taguchi analysis. The results revealed that the Taguchi method was an eff ective approach for optimizing the average electric power during the charging of lithium-ion batteries. This indicates that the positive electrode material, followed by the separator thickness and the negative electrode active material volume fraction, was key factors significantly infl uencing the average electric power during the charging of lithium-ion batteries response. The identification of optimal conditions resulted in the improved performance of lithium-ion batteries, extending their potential in various applications. Particularly, lithium-ion batteries with average electric power of 16 W and 17 W during charging were designed and simulated in the range of 0-12000 s using COMSOL Multiphysics software. This study efficiently employs the Taguchi optimization technique to develop lithium-ion batteries capable of storing a predetermined average electric power during the charging phase. Therefore, this method enables the battery to achieve complete charging within a specific timeframe tailored to a specificapplication. The implementation of this method can save costs, time, and materials compared with other alternative methods, such as the trial-and-error approach.展开更多
自引进联邦德国 MAN 公司 L+V20/27系列柴油机专利技术以来,上海新中动力机厂已累计生产100多台专利机。从 MAN 公司有关专利会议论文资料以及新中厂 L20/27机研制情况和运转一年后用户反馈信息来看,L+V20/27系列机的燃油耗率尚需进一...自引进联邦德国 MAN 公司 L+V20/27系列柴油机专利技术以来,上海新中动力机厂已累计生产100多台专利机。从 MAN 公司有关专利会议论文资料以及新中厂 L20/27机研制情况和运转一年后用户反馈信息来看,L+V20/27系列机的燃油耗率尚需进一步改善,为此,上海新中动力机厂采取了一系列技术改进措施。展开更多
文摘目的 为了开发我国珍稀动物——吉林双阳梅花鹿的基因资源 ,在分子水平上揭示鹿药材的药理作用。方法 构建了梅花鹿脾脏细胞 c DNA质粒文库 ,克隆了一些看家基因并进行了序列分析。结果 其中的一个序列在核酸及推导的氨基酸序列水平上与编码人、犬、大鼠、小鼠核蛋白体蛋白 L 2 7(ribosom al protein L 2 7) c DNA序列和对应的氨基酸序列高度同源 ,并具有完整的开放阅读框架 (ORF)。结论 发现了双阳梅花鹿核蛋白体蛋白 L2 7全长 c DNA序列 ,此序列已经在 Genbank中登录 ,登录号为 :AF3732 31。
文摘目的:研究益气活血中药复方对CVB3大鼠心肌细胞感染模型核糖体蛋白L27a(ribosomal protein L27a)表达的作用机制,进一步证实益气活血中药复方是治疗CVB3病毒性心肌炎的有效方剂。方法:本实验用新生2～3天大鼠心肌细胞建立柯萨奇病毒B3(CVB3)感染模型,通过改良的抑制性消减杂交技术(SSH),克隆了受CVB3攻击的宿主细胞中被中药(益气活血中药复方)调控的基因。结果:核糖体蛋白L27a在中药组中呈高表达,病毒组中表达减弱或被抑制。结论:益气活血中药复方能够影响受CVB3攻击的宿主细胞核糖体蛋白L27a的表达,有效保护心肌、阻断病程进度,实现治疗病毒性心肌炎的目的。
基金supported by the Applied Basic Research Programs of Science and Technology Commission Foundation of Yunnan Province(202401AT070186 to K.Q.L.,202201AS070044 to B.Z.)Yunnan Province(202305AH340006 to B.Z.)Kunming Science and Technology Bureau(2022SCP007 to B.Z.)。
文摘The DNA replication stress(RS)response is crucial for maintaining cellular homeostasis and promoting physiological longevity.However,the mechanisms by which long-lived species,such as bats,regulate RS to maintain genomic stability remain unclear.Also,recent studies have uncovered noncanonical roles of ribosome-associated factors in maintaining genomic stability.In this study,somatic skin fibroblasts from the long-lived big-footed bat(Myotis pilosus)were examined,with results showing that bat cells exhibited enhanced RS tolerance compared to mouse cells.Comparative transcriptome analysis under RS conditions revealed pronounced species-specific transcriptional differences,including robust up-regulation of ribosome biogenesis genes in bat cells and a markedly reduced activation of the P53 signaling pathway.These features emphasize a distinct homeostatic strategy in bat cells.Nuclear fragile X mental retardation-interacting protein 1(Nufip1),a ribosome-associated factor highly expressed in bat fibroblasts,was identified as a potential integrator of ribosomal and P53 signaling via its association with ribosomal protein S27-like(Rps27l).These findings provide direct cellular and molecular evidence for a noncanonical RS response in bats,highlighting a deeper understanding of the biological characteristics and genomic maintenance mechanisms of long-lived species.
文摘Different Ziegler-Natta catalysts were employed to polymerize ethylene. To investigate the influences of reaction parameters, namely Al/Ti molar ratio, hydrogen and processing parameters, i.e. ethylene pressure and temperature, a Taguchi experimental design was worked out. An L27 orthogonal array was chosen to take the above-mentioned parameters and relevant interactions into account. Response surface method was the tool used to analyze the experimental design results. Al/Ti, ethylene pressure and temperature were selected as experimental design factors, and catalyst activity and polymerization yield were the response parameters. Increasing pressure, due to an increment in monomer accessibility, and rising Al/Ti, because of higher reduction in the catalysts, cause an increase in both polymerization yield and catalyst activity. Nonetheless, a higher temperature, thanks to reducing ethylene solubility in the slurry medium and partially catalyst destruction, lead to a reduction in both response parameters. A synergistic effect was also observed between temperature and pressure. All catalyst activities will reduce in the presence of hydrogen. Molecular weight also shows a decline in the presence of hydrogen as a transfer agent. However, the polydispersity index remains approximately intact. Using SEM, various morphologies, owing to different catalyst morphologies, were seen for the polyethylene.
文摘In recent times, lithium-ion batteries have been widely used owing to their high energy density, extended cycle lifespan, and minimal self-discharge rate. The design of high-speed rechargeable lithium-ion batteries faces a significant challenge owing to the need to increase average electric power during charging. This challenge results from the direct influence of the power level on the rate of chemical reactions occurring in the battery electrodes. In this study, the Taguchi optimization method was used to enhance the average electric power during the charging process of lithium-ion batteries. The Taguchi technique is a statistical strategy that facilitates the systematic and efficient evaluation of numerous experimental variables. The proposed method involved varying seven input factors, including positive electrode thickness, positive electrode material, positive electrode active material volume fraction, negative electrode active material volume fraction, separator thickness, positive current collector thickness, and negative current collector thickness. Three levels were assigned to each control factor to identify the optimal conditions and maximize the average electric power during charging. Moreover, a variance assessment analysis was conducted to validate the results obtained from the Taguchi analysis. The results revealed that the Taguchi method was an eff ective approach for optimizing the average electric power during the charging of lithium-ion batteries. This indicates that the positive electrode material, followed by the separator thickness and the negative electrode active material volume fraction, was key factors significantly infl uencing the average electric power during the charging of lithium-ion batteries response. The identification of optimal conditions resulted in the improved performance of lithium-ion batteries, extending their potential in various applications. Particularly, lithium-ion batteries with average electric power of 16 W and 17 W during charging were designed and simulated in the range of 0-12000 s using COMSOL Multiphysics software. This study efficiently employs the Taguchi optimization technique to develop lithium-ion batteries capable of storing a predetermined average electric power during the charging phase. Therefore, this method enables the battery to achieve complete charging within a specific timeframe tailored to a specificapplication. The implementation of this method can save costs, time, and materials compared with other alternative methods, such as the trial-and-error approach.
文摘自引进联邦德国 MAN 公司 L+V20/27系列柴油机专利技术以来,上海新中动力机厂已累计生产100多台专利机。从 MAN 公司有关专利会议论文资料以及新中厂 L20/27机研制情况和运转一年后用户反馈信息来看,L+V20/27系列机的燃油耗率尚需进一步改善,为此,上海新中动力机厂采取了一系列技术改进措施。
文摘以人外周血为研究对象,研究照射后RPS27L基因的时间效应和剂量效应以及本底水平,探究其作为辐射损伤早期生物标志物的可行性。采集3名健康成人外周血,利用γ射线对其进行照射至吸收剂量分别为0、0.5、1、2、4、6和10 Gy,受照后37℃培养2、4、8、12和24 h,利用实时荧光定量PCR检测RPS27L基因的表达变化,并分析在37个健康人个体中的本底水平。结果显示,受照后的4、8、12和24 h RPS27L基因表达变化显著,而且具有很好的剂量依赖关系;在不同年龄和性别的个体中RPS27L本底水平相对稳定。因此,RPS27L基因具有作为新的辐射损伤标志物的潜力。
