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Historical Development in L2 Writing Pedagogy
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作者 叶洪 《海外英语》 2012年第9X期17-18,21,共3页
This paper reviews a succession of pedagogical orientations in second/foreign language (L2 hereafter) writing,each representing a different conception of the nature of writing.The author argues that the historical dev... This paper reviews a succession of pedagogical orientations in second/foreign language (L2 hereafter) writing,each representing a different conception of the nature of writing.The author argues that the historical development reflects persistent conflicts in the pragmatist and critical approaches in L2 writing,and calls for the search of new writing pedagogies that can satisfy both students'personal and social needs in writing. 展开更多
关键词 l2 WRITING PEDAGOGICAl development SOCIAl FUNCTION
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Molecular Cloning and Characterization of Pollen Development Related Gene RsMF2 from Raphanus sativus L.
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作者 ZHANGTao CAOJia-shu 《Agricultural Sciences in China》 CAS CSCD 2005年第7期494-500,共7页
In the paper, the full length cDNA of RsMF2 gene, homologous with the BcMF2 gene encoding pollen-specificpolygalacturonase of Chinese cabbage-pak-choi (Brassica campestris L. ssp. chinensis Makino) was cloned from Rap... In the paper, the full length cDNA of RsMF2 gene, homologous with the BcMF2 gene encoding pollen-specificpolygalacturonase of Chinese cabbage-pak-choi (Brassica campestris L. ssp. chinensis Makino) was cloned from Raphanussativus L. cv. Yuanbai by PCR, with a pair of primer designed according to the coding sequence of BcMF2. The largestopening reading frame of RsMF2 gene is 1 266 bp in length and encodes a protein of 421 amino acids with a predictedmolecular mass of 43.9 kDa. Sequence analysis revealed that it has three potential N-glycosylation sites and onepolygalacturonase active position (RVTCGPGHGLSVGS). And the first 32 amino acids of the predicted RsMF2 proteinform a N-terminal hydrophobic domain which displays the properties of a signal peptide. The predicted secondarystructure composition for the protein has 6.9% helix, 42.0% sheet and 51.1% loop. Four domains which are highly conservedin the whole plant and fungal PGs is present in RsMF2. Phylogenetic analysis showed that RsMF2 falls into the categoryof clade-C, which includes PGs related to pollen. These results indicate that RsMF2 may act as polygalacturonase relatedto pollen development. 展开更多
关键词 Raphanus sativus l. RsMF2 Pollen development ClONING CHARACTERIZATION
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Intercultural Competence Development of Chinese Students After a Short-Term Study Abroad Experience 被引量:1
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作者 Shuangmei Gao Teresa J. Kennedy 《Sino-US English Teaching》 2019年第5期177-196,共20页
This study investigated Chinese students’ intercultural competence and linguistic growth during a six-week study abroad program. The students and their professor participated in pre- and post-tests covering written a... This study investigated Chinese students’ intercultural competence and linguistic growth during a six-week study abroad program. The students and their professor participated in pre- and post-tests covering written and oral language competency as well as intercultural competence. The Global Scale of English assessed student language development to determine linguistic growth and the Assessment of Intercultural Competence of Chinese College Students (AIC-CCS) was used to determine student growth of intercultural competence skills, specifically related to cultural knowledge, skills, attitudes, and awareness as a result of their experiences in a non-Western context. Results indicated that students experienced a change in the salience of intercultural knowledge themes over their period abroad, as well as improved their individual language abilities. This study provides suggestions for future programming focused on the development of Chinese student intercultural competencies during short-term study abroad experiences in Western university environments. 展开更多
关键词 ENGlISH lANGUAGE development linguistic growth Global Scale of ENGlISH second lANGUAGE (l2) INTERCUlTURAl COMMUNICATIVE competence (ICC) CHINESE college students CHINESE context
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Development of EFL Students' Writing in Secondary Education
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作者 Ana Cristina Lahuerta Martinez Alberto Isusi Alabarte 《Journal of Linguistics and Education Research》 2018年第1期1-13,共13页
The aim of this paper is to explore and measure language learners'performance in L2 writing production using the complexity,accuracy,and fluency constructs.A total of 123 secondary education students took part in ... The aim of this paper is to explore and measure language learners'performance in L2 writing production using the complexity,accuracy,and fluency constructs.A total of 123 secondary education students took part in the study.Results are manifold.In the first place,they show that the measures of fluency,accuracy,grammatical and lexical complexity progress in a significant way:fourth grade students outperform first graders in the aforementioned measures.Secondly,fewer correlations between the writing measures used and the general quality of the compositions are found among the older students than among the younger ones,indicating that the correlations change depending on learners'age.Thirdly,1st year students exhibit a higher ratio of errors,both in general and also by error category,although only two types decrease significantly in 4th year students:syntactic and spelling errors.Lastly,we find that errors tend to develop in a non-linear way. 展开更多
关键词 l2 writing development Accuracy FlUENCY GRAMMATICAl COMPlEXITY lEXICAl COMPlEXITY Secondary education
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The Relation Between L1 and L2
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作者 王慧 《海外英语》 2017年第12期218-219,236,共3页
The distinctions between L1 acquisition and L2 acquisition have always been one of the significant factors in the language acquisition field. we all know the fact that different learners in different situations learn ... The distinctions between L1 acquisition and L2 acquisition have always been one of the significant factors in the language acquisition field. we all know the fact that different learners in different situations learn L2 s in different ways. The paper compares the natural route of development between L1 and L2 and the result demonstrates the relation between L1 and L2, to be exact, the similarities and differences. 展开更多
关键词 l1 and l2 similarities and differences natural process of development
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环境激素2,4-二氯苯酚对家蚕生殖发育的影响 被引量:3
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作者 袁红霞 裔洪根 +1 位作者 陈息林 徐世清 《农业环境科学学报》 CAS CSCD 北大核心 2009年第11期2259-2263,共5页
为了探讨环境激素对鳞翅目昆虫的影响,用添加2,4-二氯苯酚(2,4-DCP)的人工饲料饲养家蚕,调查2,4-DCP对家蚕生殖发育的影响。结果显示,1.60 mmol.kg-1以下浓度的2,4-DCP,对幼虫和蛹的卵巢生长有促进作用(P<0.05);1.60 mmol.kg-1的2,4-... 为了探讨环境激素对鳞翅目昆虫的影响,用添加2,4-二氯苯酚(2,4-DCP)的人工饲料饲养家蚕,调查2,4-DCP对家蚕生殖发育的影响。结果显示,1.60 mmol.kg-1以下浓度的2,4-DCP,对幼虫和蛹的卵巢生长有促进作用(P<0.05);1.60 mmol.kg-1的2,4-DCP对5龄后期快速生长阶段的卵巢表现出抑制作用,但在蛹期卵巢的生长获得补偿。高浓度的2,4-DCP对幼虫期、蛹期卵细胞的生长和发育有一定的促进作用(P<0.01)。2,4-DCP对家蚕雄性生殖腺的生长、特别是5龄后期精巢的快速生长期表现出一定的抑制作用,在蛹期抑制作用表现十分强烈,1.60 mmol.kg-1的2,4-DCP使雄蛾蚕的精巢几乎完全退化。2,4-DCP使5龄幼虫的精细胞数量微弱减少,至蛹期精细胞和蛾期精子的数量则显著少于对照(P<0.01)。1.60 mmol.kg-1的2,4-DCP使雌蛾的产卵数下降到对照的20%,但造卵数比对照高1.3倍,0.80 mmol.kg-1以上浓度的2,4-DCP使不受精卵率显著增高(P<0.05)。2,4-DCP主要通过强烈抑制家蚕雄性生殖腺和生殖细胞的发育以表现出很强的雌激素效应。 展开更多
关键词 环境激素 2 4-二氯苯酚 鳞翅目昆虫 家蚕 生殖发育
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杂种鹅掌楸花粉发育过程中细胞游离Ca^(2+)的动态变化 被引量:1
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作者 尹增芳 宁代锋 +2 位作者 李玉春 席梦利 施季森 《林业科学》 EI CAS CSCD 北大核心 2007年第11期27-31,175-176,共7页
利用钙离子荧光探针Fluo-3/AM低温装载技术,在激光扫描共聚焦显微镜下观察杂种鹅掌楸花粉发育不同阶段细胞内游离Ca2+的分布,以其相对荧光强度的强弱来衡量细胞内游离Ca2+水平的动态变化。研究发现在初生造孢细胞中Ca2+荧光较弱,次生造... 利用钙离子荧光探针Fluo-3/AM低温装载技术,在激光扫描共聚焦显微镜下观察杂种鹅掌楸花粉发育不同阶段细胞内游离Ca2+的分布,以其相对荧光强度的强弱来衡量细胞内游离Ca2+水平的动态变化。研究发现在初生造孢细胞中Ca2+荧光较弱,次生造孢细胞和小孢子母细胞时期Ca2+荧光增强。早期四分体小孢子细胞质中Ca2+荧光强度较强,胼胝质壁无荧光;后期细胞质中的Ca2+荧光减弱,而胼胝质壁处Ca2+荧光增强。小孢子内Ca2+分布不均匀,细胞质中的Ca2+荧光较弱,细胞壁处Ca2+荧光较强,二细胞花粉时期,细胞呈现较强的Ca2+荧光。在花药壁组织内Ca2+分布也呈现规律性的变化:造孢组织时期,花药壁组织Ca2+荧光强度在不同壁层组织中分布均匀;小孢子母细胞时期,药壁中层细胞Ca2+荧光最强,绒毡层细胞次之;单核小孢子时期,绒毡层细胞呈解体状态,Ca2+荧光最强,并保持到二核花粉时期直至绒毡层完全消失,但此时花药纤维层发育形成,表现出较强的Ca2+荧光。花药组织中Ca2+分布动态显示出Ca2+由外而内流动的迹象,而且细胞内游离Ca2+分布特征与花粉发育过程的重要转变环节密切相关。 展开更多
关键词 杂种鹅掌楸 花粉发育 游离CA^2%PlUS% 低温装载
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6-BA和2,4-D对黄独带芽茎段生长发育的影响 被引量:1
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作者 洪森荣 程细云 《南方农业学报》 CAS CSCD 北大核心 2012年第3期286-289,共4页
【目的】探讨6-BA和2,4-D对黄独带芽茎段生长发育的影响,为黄独的产业化生产提供理论依据。【方法】采用植物组织培养的方法,将黄独带芽茎段(0.5~1.5cm)接种至液体培养基进行单因子试验。【结果】黄独带芽茎段芽增殖的最佳培养基为MS;... 【目的】探讨6-BA和2,4-D对黄独带芽茎段生长发育的影响,为黄独的产业化生产提供理论依据。【方法】采用植物组织培养的方法,将黄独带芽茎段(0.5~1.5cm)接种至液体培养基进行单因子试验。【结果】黄独带芽茎段芽增殖的最佳培养基为MS;黄独带芽茎段芽增长的最佳培养基为MS+6-BA1mg/L+2,4-D0.5mg/L;黄独带芽茎段芽增粗的最佳培养基为MS+6-BA2mg/L+2,4-D0.1mg/L;黄独带芽茎段生根的最佳培养基为MS;黄独带芽茎段茎长增加的最佳培养基为MS+6-BA2mg/L+2,4-D0.1mg/L;黄独带芽茎段茎粗增加的最佳培养基为MS。【结论】黄独带芽茎段的芽增殖和生根最好不要使用6-BA与2,4-D组合,但黄独带芽茎段如在其他培养基诱导出新芽后,则可利用6-BA与2,4-D组合来促进芽的增长、增粗以及茎长的增加。 展开更多
关键词 黄独 带芽茎段 6-BA 2 4-D 生长发育
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2-萘酚及其衍生产品合成工艺进展 被引量:7
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作者 梁诚 《染料工业》 2001年第5期17-19,25,共4页
本文介绍了2-萘酚及其衍生物吐氏酸、J酸、2,3酸的合成技术进展,并就它们的合成技术、三废处理及技术发展方向等作简述。
关键词 2-萘酚 吐氏酸 J酸 2 3酸 技术进展 合成工艺
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光敏核不育水稻农垦58S花粉败育过程中Ca^(2+)-ATPase的变化 被引量:1
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作者 史银连 邓芳 +1 位作者 曹剑波 姚家玲 《作物学报》 CAS CSCD 北大核心 2008年第12期2106-2111,共6页
运用电镜细胞化学技术(铅盐沉淀法),对长日照条件下光敏核不育水稻农垦58S和可育株系58N花药发育的不同时期进行了Ca2+-ATPase定位研究。结果表明,Ca2+-ATPase颗粒在可育与不育水稻的花药壁、花粉及药隔中的出现时间和数量具有明显差异... 运用电镜细胞化学技术(铅盐沉淀法),对长日照条件下光敏核不育水稻农垦58S和可育株系58N花药发育的不同时期进行了Ca2+-ATPase定位研究。结果表明,Ca2+-ATPase颗粒在可育与不育水稻的花药壁、花粉及药隔中的出现时间和数量具有明显差异。可育花药在花粉母细胞时期,药壁细胞内出现Ca2+-ATPase颗粒,至单核花粉后期,解体的绒毡层细胞和乌氏体表面分布大量的Ca2+-ATPase颗粒;与可育材料相比,不育花药在花粉发育的同时期,Ca2+-ATPase颗粒在药壁各层细胞内不仅出现的时间滞后,且分布的数量较少,到二核花粉时期(花粉已畸形空瘪),药壁表皮细胞的液泡膜上、绒毡层细胞质中及乌氏体表面才出现明显的Ca2+-ATPase颗粒。可育花粉单核早期,花粉细胞内线粒体膜上有少量的Ca2+-ATPase分布;单核花粉中期,花粉外壁上有少量的Ca2+-ATPase分布;到单核花粉后期,Ca2+-ATPase颗粒大量分布在花粉外壁、内壁及细胞质膜和细胞核中。而不育花粉在单核花粉发育中都未见Ca2+-ATPase的分布,到花粉败育空瘪时才出现明显的Ca2+-ATPase颗粒,但数量较可育花粉同期同部位少。可育花药药隔细胞中Ca2+-ATPase在花粉母细胞减数分裂期就有分布,而不育花药到单核花粉早期药隔中才有少量的Ca2+-ATPase分布。由此推测,水稻不育系在其花粉发育中,由于细胞壁和质膜上Ca2+-ATPase颗粒出现时间滞后和数量减少影响到细胞膜钙泵将Ca2+由胞质向胞外转运的功能,致使胞质内Ca2+过多积累,导致花粉败育。 展开更多
关键词 光敏雄性核不育 水稻 花药发育 CA^2%PlUS%-ATPASE
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斑马鱼和鲤miR-1-2/133a-1基因间序列活性和调控研究
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作者 龚葭薇 何梅 +2 位作者 闫学春 孔德麟 梁洋 《水生生物学报》 CAS CSCD 北大核心 2021年第3期507-513,共7页
分别以斑马鱼(Danio rerio)和鲤(Cyprinus carpio L.)为研究材料,通过克隆斑马鱼和鲤miR-1-2和133a-1的基因间增强子序列,利用活体和离体实验探讨其是否具有肌肉特异性;并通过荧光素酶报告系统等研究转录因子MyoD是否调控该序列。研究... 分别以斑马鱼(Danio rerio)和鲤(Cyprinus carpio L.)为研究材料,通过克隆斑马鱼和鲤miR-1-2和133a-1的基因间增强子序列,利用活体和离体实验探讨其是否具有肌肉特异性;并通过荧光素酶报告系统等研究转录因子MyoD是否调控该序列。研究结果显示:在活体实验中,无论斑马鱼还是鲤, miR-1-2/133a-1基因间序列均有肌肉特异性,且保留有保守区域(cr,含有E-box)的序列,注射72h后GFP表达量明显高于其他突变体。体外细胞实验也显示,转染含有cr序列的实验组,分化后的荧光素酶活性明显高于分化前。进一步探讨MyoD对miR-1-2/133a-1基因间序列的调控作用结果显示:无论是斑马鱼还是鲤, miR-1-2/133a-1基因间序列活性均受MyoD调控。结果为完善鱼类肌肉发育机理,及未来的鱼类分子育种奠定基础。 展开更多
关键词 斑马鱼 肌肉发育 MYOD miR-1-2/133a-1 基因间序列
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Task Repetition and the Development of L2 Oral Production
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作者 ZHI Na LI Aijun JIA Yuan 《中国语音学报》 2016年第1期62-70,共9页
The paper reports the developmental trajectory of Chinese EFL( English as a foreign language) learners’ oral performance in terms of their speech quantity and quality in repeated topic-based tasks. It has been a conc... The paper reports the developmental trajectory of Chinese EFL( English as a foreign language) learners’ oral performance in terms of their speech quantity and quality in repeated topic-based tasks. It has been a concerned issue in the theoretical and pedagogical interests that how L2 learners can enhance their oral proficiency. However,as pointed in[22],there are few studies focused particularly on the L2 speakers’ performance change,while more studies are devoted to the performance status. The current research presents a case study of six Chinese students’ English oral development addressing to the issue of learners’ dynamic change. By investigating each speaker’s variation in the speech quantity( the total amount of produced speech) and speech quality( indicated by fluency,accuracy and complexity) during the three-week intervals,we find that task repetition can positively affect the speakers’ oral production. 展开更多
关键词 TASK REPETITION the development l2 ORAl PRODUCTION
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欧文氏菌和棒杆菌的属间融合研究 被引量:26
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作者 林红雨 陈策实 尹光琳 《微生物学通报》 CAS CSCD 北大核心 1999年第1期3-6,共4页
研究了用原生质体融合技术获得欧文氏菌和棒杆菌的融合细胞。串联发酵D-葡萄糖产生2-酮基-L-古龙酸的第一步发酵菌株欧文氏菌SCB247经0.8mg/mL溶菌酶酶解0.5h后,原生质体的形成率和再生率分别为99.8%和... 研究了用原生质体融合技术获得欧文氏菌和棒杆菌的融合细胞。串联发酵D-葡萄糖产生2-酮基-L-古龙酸的第一步发酵菌株欧文氏菌SCB247经0.8mg/mL溶菌酶酶解0.5h后,原生质体的形成率和再生率分别为99.8%和27.8%。第二步发酵菌株棒杆菌SCB3058经预处理后由1.3mg/mL溶菌酶酶解2h,原生质体的形成率和再生率分别为99.5%和56.3%。用携带氨苄青霉素抗性标记的SCB247和经热灭活的SCB3058为亲本,在40%PEG6000和0.2mol/L新生磷酸钙等适宜条件下融合,融合频率为3.6×10^(-6)。在非选择和选择培养基上连续传代十几次后,对融合子的单菌形态、染色结果、菌落形态、色泽、总蛋白量、同工酶、发酵性能等方面与亲本进行了比较。结果表明,融合子确系棒杆菌和欧文氏菌的融合细胞。摇瓶发酵结果显示,所得的38株稳定的融合子中约40%能转化葡萄糖为维生素C前体2-酮基-L-古龙酸。 展开更多
关键词 原生质体 融合子 棒杆菌 欧文氏菌
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枸杞脂肪酰基还原酶基因的克隆及表达分析 被引量:2
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作者 石晶 石鑫强 +4 位作者 管翠萍 郑蕊 杨亚珺 王丽娟 梁文裕 《植物遗传资源学报》 CAS CSCD 北大核心 2018年第2期351-360,共10页
脂肪酰基还原酶基因广泛参与植物的脂类代谢过程,影响植物雄配子体花药发育以及表皮蜡酯合成等。本研究利用RACE方法从宁夏枸杞(宁杞1号)花药中克隆脂肪酰基还原酶LbMS2-2基因,开放阅读框全长1800bp,编码599个氨基酸,等电点为9.00。生... 脂肪酰基还原酶基因广泛参与植物的脂类代谢过程,影响植物雄配子体花药发育以及表皮蜡酯合成等。本研究利用RACE方法从宁夏枸杞(宁杞1号)花药中克隆脂肪酰基还原酶LbMS2-2基因,开放阅读框全长1800bp,编码599个氨基酸,等电点为9.00。生物信息学分析表明,LbMS2-2蛋白定位于叶绿体中,该蛋白序列与茄科植物甜辣椒、烟草和马铃薯中的脂肪酰基还原酶表现出较高的序列相似性;实时荧光定量PCR显示,LbMS2-2基因在枸杞花器官中表达,且在枸杞花药发育的四分体时期、单核花粉时期和双核花粉时期表达量较高。原位杂交结果证实该基因只在花药绒毡层和小孢子中表达。亚细胞定位结果进一步验证LbMS2-2基因的叶绿体定位。以上结果表明,枸杞脂肪酰基还原酶基因是枸杞花器官发育过程中的重要基因。 展开更多
关键词 枸杞 花药发育 脂肪酰基还原酶 lbMS2-2 表达分析
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留坝西洋参栽培制约性问题研究与产业发展模式探索 被引量:3
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作者 田小曼 胡小平 +2 位作者 陈志敏 苏景文 刘德明 《陕西农业科学》 2022年第4期88-92,共5页
西洋参是我国引种的贵重中药材,留坝是我国七五攻关成功引种西洋参的三大种植基地之一,留坝西洋参品质是最接近北美洲原产地的引种西洋参。近年来,留坝西洋参连作障碍问题直接制约产业健康发展,引发栽培区经济社会问题,并对生态环境造... 西洋参是我国引种的贵重中药材,留坝是我国七五攻关成功引种西洋参的三大种植基地之一,留坝西洋参品质是最接近北美洲原产地的引种西洋参。近年来,留坝西洋参连作障碍问题直接制约产业健康发展,引发栽培区经济社会问题,并对生态环境造成威胁。本文通过西洋参栽培综合技术研究示范推广、“2+2”栽培模式、全产业链产品开发等措施,探索构建留坝西洋参产业发展新模式,以期为连作障碍问题严重、生态环境脆弱区域产业可持续发展提供借鉴。 展开更多
关键词 留坝西洋参 连作障碍 2%PlUS%2”栽培模式 可持续发展
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IS NOTICING VITAL FOR L2 LEARNING?——A CRITICAL REVIEW OF SCHMIDT’S "NOTICING HYPOTHESIS" 被引量:2
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作者 温植胜 《Chinese Journal of Applied Linguistics》 2008年第3期3-8,127,共7页
二语习得研究学者Schmidt提出的"注意假设"认为,"注意是二语发展的前提条件"。本文作者对此观点提出质疑。文章首先根据二语习得研究的输入理论指出该假设在理论方面存在片面性;然后进一步指出该假设所引证的Schmid... 二语习得研究学者Schmidt提出的"注意假设"认为,"注意是二语发展的前提条件"。本文作者对此观点提出质疑。文章首先根据二语习得研究的输入理论指出该假设在理论方面存在片面性;然后进一步指出该假设所引证的Schmidt自身的研究以及其他二语习得研究者的相关研究均未能支持该假设的成立。作者最后指出,尽管"注意假设"对外语教学与研究带来一定程度的启示作用,由于这方面的实证研究数目相当有限,而且在研究方法上也有待改善,因此Schmidt关于"注意是二语发展的前提条件"之假设不能成立。语言学界仍需继续深入探讨二语习得所牵涉的复杂认知心理过程。 展开更多
关键词 noticing hypothesis l2 interlanguage development l2 cognitive processes
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以主要流行EV71 VP1基因高度保守区为靶点的TaqMan荧光定量PCR检测方法的建立 被引量:4
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作者 胡晓星 彭杰 +3 位作者 冯悦 刘丽 张阿梅 夏雪山 《生命科学研究》 CAS CSCD 2016年第3期189-195,共7页
在我国,肠道病毒71型(enterovirus 71,EV71)C4型是引起手足口病的主要流行基因型。为建立EV71C4型TaqMan荧光定量PCR检测方法,在C4型EV71VP1基因的高保守区,设计合成引物和TaqMan探针,将包含此目的区段的基因片段克隆到pcDNA3.1载体中,... 在我国,肠道病毒71型(enterovirus 71,EV71)C4型是引起手足口病的主要流行基因型。为建立EV71C4型TaqMan荧光定量PCR检测方法,在C4型EV71VP1基因的高保守区,设计合成引物和TaqMan探针,将包含此目的区段的基因片段克隆到pcDNA3.1载体中,通过体外转录获得标准品,并以梯度稀释的标准品为模板建立工作曲线,进而在优化反应条件的基础上建立TaqMan荧光实时定量PCR检测方法。实验中,所设计引物、探针的高度保守性保证了C4型EV71的高效扩增。经反应条件优化,引物和探针的最佳工作浓度分别为300 nmol/L和200 nmol/L,在1×10~301×10~3拷贝数检测范围内具有良好的线性关系(R^2=1),灵敏度可达到10~2 copies/μL。通过对该方法进行检验发现,批间和组间重复实验的变异系数均小于0.5%,且该方法对柯萨奇A16(coxsackievirus A16,CA16)柯萨奇B1(coxsackievirus B1,CB1)人轮状病毒(human rotavirus,HRV)单纯疱疹病毒2型(herpes Simplex virus type 2,HSV-2)均无交叉反应,对6份EV71阳性样本检出率为100%。以上数据表明,文中建立的TaqMan荧光定量PCR方法可为我国主要流行C4型EV71感染的快速诊断及疾病监控提供有效途径。 展开更多
关键词 肠道病毒71型 VP1基因 高保守区 TAQMAN探针 荧光定量PCR 检测
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A Malvaceae-specific miRNA targeting the newly duplicated GaZIP1L to regulate Zn^(2+)ion transporter capacity in cotton ovules 被引量:5
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作者 Xingpeng Wen Gai Huang +1 位作者 Chenyu Li Yuxian Zhu 《Science China(Life Sciences)》 SCIE CAS CSCD 2021年第3期339-351,共13页
MicroRNAs(miRNAs)play critical roles in regulating gene expression in plants,yet their functions underlying cultivated diploid Gossypium arboreum cotton ovule development are largely unknown.Here,we acquired small RNA... MicroRNAs(miRNAs)play critical roles in regulating gene expression in plants,yet their functions underlying cultivated diploid Gossypium arboreum cotton ovule development are largely unknown.Here,we acquired small RNA profiles from G.arboreum ovules and fibers collected at different growth stages,and identified 46 novel miRNAs that accounted for 23.7%of all miRNAs in G.arboreum reported in the latest plant sRNA database.Through analysis of 84(including 38 conserved)differentially expressed G.arboreum miRNAs,we detected 215 putative protein-coding genes in 26 biological processes as their potential targets.A Malvaceae-specific novel miRNA named gar-miRN44 was found to likely regulate cotton ovule growth by targeting to a newly duplicated Zn^(2+)ion transporter gene GaZIP1L.During cotton ovule development,gar-miRN44 transcript level decreased sharply after 10 to 15 days post-anthesis(DPA),while that of the GaZIP1L increased significantly,with a concomitant increase of Zn^(2+)ion concentration in late ovule developmental stages.Molecular dynamics simulation and ion absorption analysis showed that GaZIP1L has stronger Zn2+ion binding ability than the original GaZIP1,indicating that the newly evolved GaZIP1L may be more suitable for maintaining high Zn2+ion transport capacity that is likely required for cotton ovule growth via enhanced cellulose synthase activities.Our systematic miRNA profiling in G.arboreum and characterization of gar-miRN44 not only contribute to the understanding of miRNA function in cotton,but also provide potential targets for plant breeding. 展开更多
关键词 gar-miRN44 MAlVACEAE GaZIP1l Zn^(2%PlUS%)ion transport cotton ovule development
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Developing Chinese Complimenting in a Study Abroad Program
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作者 金丽 《Chinese Journal of Applied Linguistics》 2015年第3期277-300,368,共25页
This article reports on an empirical study investigating what and how two college-level American learners of Mandarin Chinese developed their own understanding of the Chinese complimenting speech act while participati... This article reports on an empirical study investigating what and how two college-level American learners of Mandarin Chinese developed their own understanding of the Chinese complimenting speech act while participating in a 7-week intensive language study abroad program. A case study approach was adopted with a focus on participants' self-reflection data supplemented with the researcher's observation data to unpack each participant's pragmatic developmental process throughout the program. Guided by sociocultural theory, the study uncovered the dynamic, complex and highly individualized developmental process each participant experienced. The findings revealed that Chinese people's special treatment of the two American students provided more constraints than opportunities for them to acquire Chinese complimenting. However, the learners' own motivation and approaches to learning also significantly shaped their learning process and outcomes. Pedagogical suggestions for Chinese pragmatic development in study abroad contexts are provided. 展开更多
关键词 l2 Pragmatic development Study Abroad Mandarin Chinese Complimenting American College-level learners of Chinese
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A CROSS-SECTIONAL STUDY OF CHINESE LEARNERS' ACQUISITION OF ENGLISH REQUESTS 被引量:1
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作者 杨仙菊 《Chinese Journal of Applied Linguistics》 2008年第6期31-43,127,共14页
Following a cross-sectional design, the present study investigated the acquisition of English requests by Chinese learners at three proficiency levels with open production questionnaires. It was found that L2 linguist... Following a cross-sectional design, the present study investigated the acquisition of English requests by Chinese learners at three proficiency levels with open production questionnaires. It was found that L2 linguistic proficiency, L1 pragmatic transfer and classroom instruction have interactive effect on learners' L2 pragmatic development. A range of acquisitional features were found-learners' employment of direct request strategies decreased, the employment of conventionally indirect strategies increased and the number and variety of internal modifiers increased with the increase of proficiency. It was also found that L1 pragmatic transfer worked on various aspects of learners' requestive behavior and that inadequate or inappropriate input from the teaching materials and the EFL classroom arrangements might constrain their pragmatic development. 展开更多
关键词 Interlanguage Pragmatics speech act of requests l2 pragmatic development cross-sectional design
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