[Objective] The purpose of this study is to determine the effects of com-bined use of boron and manganese fertilizers on the nutritional quality and physio-logical indices of Brassica campestris. [Method] In the nutri...[Objective] The purpose of this study is to determine the effects of com-bined use of boron and manganese fertilizers on the nutritional quality and physio-logical indices of Brassica campestris. [Method] In the nutrient solutions for growing B. campestris by hydroponics, boric acid and manganese sulfate were added at 0.5, 2.5, and 7.5 mg/L respectively. Another treatment without boron and manganese was prepared as the control. Quality and physiological indices of B. campestris in the 10 treatments were measured. [Result] Boron and manganese shared obvious in-teraction in improving the quality and physiological indices of B. campestris. To cul-tivate B. campestris with high quality and strong resistance, the optimum concentra-tions of boron and manganese in the nutrient solution should be 2.5 mg/L boric acid and 2.5-7.5 mg/L manganese sulfate. [Conclusion] The findings wil provide refer-ence for studying effects of trace elements on nutrient composition of vegetables.展开更多
Objectives:Among cutaneous malignancies,melanoma stands out for its particularly aggressive nature,with therapeutic interventions becoming notably limited once the disease progresses.In this research,we investigate th...Objectives:Among cutaneous malignancies,melanoma stands out for its particularly aggressive nature,with therapeutic interventions becoming notably limited once the disease progresses.In this research,we investigate the tumor-suppressing capabilities of water-extracted Korean mistletoe(Viscum album var.coloratum)and its purified lectin component(V.album var.coloratum agglutinin,VCA)using two distinct mouse melanoma models:B16BL6 and B16F10 cell lines.Methods:The impact of water extract and VCA treatments on melanoma cells was assessed through multiple experimental approaches,examining cellular survival rates,programmed cell death pathways,multicaspase activity,and cell cycle distribution patterns.To elucidate the interconnections among various cellular responses,we employed a suite of statistical techniques encompassing correlation studies,principal component analysis(PCA)-based dimensionality reduction,and dendrogram-based clusteringmethodologies.Results:Thewater extract exhibited dosedependent cytotoxicity with IC50 values of 372.3±8.7μg/mL and 202.5±8.4μg/mL for B16BL6 and B16F10 cells,respectively.VCA showed more significant effects,with IC50 values of 0.1992±0.0041 and 0.1981±0.0098μg/mL for B16BL6 and B16F10 cells,respectively.Both agents induced substantial apoptosis with a significant progression from early to late apoptotic stages,reaching up to 59.4%total apoptotic cells for VCA treatment.This was confirmed by strong multicaspase activation,particularly in VCA-treated cells(up to 88.4%caspase-positive cells).The water extract showed modest effects on cell cycle distribution,with increases in G0/G1 phase(74.6%)in B16BL6 cells and S phase(19.2%)in B16F10 cells,while VCA treatment resulted in G2/M phase reduction(10.0%)in B16F10 cells.Correlation analysis revealed strong negative associations between cell viability and caspase activity(r=−0.843 to−0.878),while hierarchical clustering demonstrated distinct response patterns between low and high concentrations of both agents.Effect size analysis confirmed strong treatment impacts on cell viability(d=−5.89 to−6.12)and caspase activation(d=3.45 to 5.23).Conclusion:These findings suggest that Korean mistletoe water extract and its isolated lectin may affect both primary and metastatic melanoma cells through distinct mechanisms,demonstrating particular potency in caspase-dependent apoptosis induction.Our findings establish a robust foundation for developing novel therapeutic interventions derived from natural compounds to combat malignant melanoma with high metastatic potential.展开更多
The factors which effected the extraction of total flavones from Polygonum aviculare L. var. aviculare were discussed with respects to solvent, concentration and the extracting temperature. The rutin was used as a sta...The factors which effected the extraction of total flavones from Polygonum aviculare L. var. aviculare were discussed with respects to solvent, concentration and the extracting temperature. The rutin was used as a standard compound, the total flavones extracted by the different extracting procedure were quantitated by measurement of absorbance at 510 nm. The results show that 80 ℃ and 35% alcohol are optimal and the extracting rate of the total flavones is as high as 4.349%.展开更多
基金Supported by a grant from Ministry of Science and Technology for the Project of Science and Technology Talents Serving in Enterprise(2009GJC50042)~~
文摘[Objective] The purpose of this study is to determine the effects of com-bined use of boron and manganese fertilizers on the nutritional quality and physio-logical indices of Brassica campestris. [Method] In the nutrient solutions for growing B. campestris by hydroponics, boric acid and manganese sulfate were added at 0.5, 2.5, and 7.5 mg/L respectively. Another treatment without boron and manganese was prepared as the control. Quality and physiological indices of B. campestris in the 10 treatments were measured. [Result] Boron and manganese shared obvious in-teraction in improving the quality and physiological indices of B. campestris. To cul-tivate B. campestris with high quality and strong resistance, the optimum concentra-tions of boron and manganese in the nutrient solution should be 2.5 mg/L boric acid and 2.5-7.5 mg/L manganese sulfate. [Conclusion] The findings wil provide refer-ence for studying effects of trace elements on nutrient composition of vegetables.
文摘Objectives:Among cutaneous malignancies,melanoma stands out for its particularly aggressive nature,with therapeutic interventions becoming notably limited once the disease progresses.In this research,we investigate the tumor-suppressing capabilities of water-extracted Korean mistletoe(Viscum album var.coloratum)and its purified lectin component(V.album var.coloratum agglutinin,VCA)using two distinct mouse melanoma models:B16BL6 and B16F10 cell lines.Methods:The impact of water extract and VCA treatments on melanoma cells was assessed through multiple experimental approaches,examining cellular survival rates,programmed cell death pathways,multicaspase activity,and cell cycle distribution patterns.To elucidate the interconnections among various cellular responses,we employed a suite of statistical techniques encompassing correlation studies,principal component analysis(PCA)-based dimensionality reduction,and dendrogram-based clusteringmethodologies.Results:Thewater extract exhibited dosedependent cytotoxicity with IC50 values of 372.3±8.7μg/mL and 202.5±8.4μg/mL for B16BL6 and B16F10 cells,respectively.VCA showed more significant effects,with IC50 values of 0.1992±0.0041 and 0.1981±0.0098μg/mL for B16BL6 and B16F10 cells,respectively.Both agents induced substantial apoptosis with a significant progression from early to late apoptotic stages,reaching up to 59.4%total apoptotic cells for VCA treatment.This was confirmed by strong multicaspase activation,particularly in VCA-treated cells(up to 88.4%caspase-positive cells).The water extract showed modest effects on cell cycle distribution,with increases in G0/G1 phase(74.6%)in B16BL6 cells and S phase(19.2%)in B16F10 cells,while VCA treatment resulted in G2/M phase reduction(10.0%)in B16F10 cells.Correlation analysis revealed strong negative associations between cell viability and caspase activity(r=−0.843 to−0.878),while hierarchical clustering demonstrated distinct response patterns between low and high concentrations of both agents.Effect size analysis confirmed strong treatment impacts on cell viability(d=−5.89 to−6.12)and caspase activation(d=3.45 to 5.23).Conclusion:These findings suggest that Korean mistletoe water extract and its isolated lectin may affect both primary and metastatic melanoma cells through distinct mechanisms,demonstrating particular potency in caspase-dependent apoptosis induction.Our findings establish a robust foundation for developing novel therapeutic interventions derived from natural compounds to combat malignant melanoma with high metastatic potential.
文摘The factors which effected the extraction of total flavones from Polygonum aviculare L. var. aviculare were discussed with respects to solvent, concentration and the extracting temperature. The rutin was used as a standard compound, the total flavones extracted by the different extracting procedure were quantitated by measurement of absorbance at 510 nm. The results show that 80 ℃ and 35% alcohol are optimal and the extracting rate of the total flavones is as high as 4.349%.
