Insulin resistance(IR)is a central event in the pathogenesis of type 2 diabetes mellitus(T2DM).In recent years,the global burden of T2DM has intensified,with conventional therapeutic agents carrying certain side effec...Insulin resistance(IR)is a central event in the pathogenesis of type 2 diabetes mellitus(T2DM).In recent years,the global burden of T2DM has intensified,with conventional therapeutic agents carrying certain side effects.Therefore,there is an urgent need to identify safer and more effective alternative therapies,which has accelerated research on natural green plants.Lonicera caerulea var.edulis is abundant in diverse bioactive constituents,however,the hypoglycemic potential of its important bioactive component—polysaccharides—has not yet been fully exploited.Within this investigation,a native polysaccharide(LP)was separated from L.caerulea var.edulis.Structural analysis revealed that LP was an acidic heteropolysaccharide possessing a molecular weight of 109.64 kDa,consisting mainly of galacturonic acid,galactose,and arabinose.Additionally,LP contained both HG and RG-I domains and showed a porous layered structure with favorable thermal stability.In insulin resistant HepG2(IR-HepG2)cell model,LP ameliorated insulin resistance,as evidenced by increased glucose consumption(up to 1.39-fold),glycogen synthesis(up to 2.34-fold),and enhanced activities of hexokinase(up to 1.48-fold)and pyruvate kinase(up to 1.98-fold)relative to the model group,through modulation of the IRS-1/PI3K/Akt signaling pathway.Notably,at concentrations of 100-200μg/mL,LP demonstrated significant hypoglycemic effects,markedly improving these metabolic parameters and thereby alleviating insulin resistance.These findings confirm LP's capability to regulate T2DM,establishing a scientific basis for the prospective use of L.caerulea var.edulis as a promising functional food resource.展开更多
Objectives:Among cutaneous malignancies,melanoma stands out for its particularly aggressive nature,with therapeutic interventions becoming notably limited once the disease progresses.In this research,we investigate th...Objectives:Among cutaneous malignancies,melanoma stands out for its particularly aggressive nature,with therapeutic interventions becoming notably limited once the disease progresses.In this research,we investigate the tumor-suppressing capabilities of water-extracted Korean mistletoe(Viscum album var.coloratum)and its purified lectin component(V.album var.coloratum agglutinin,VCA)using two distinct mouse melanoma models:B16BL6 and B16F10 cell lines.Methods:The impact of water extract and VCA treatments on melanoma cells was assessed through multiple experimental approaches,examining cellular survival rates,programmed cell death pathways,multicaspase activity,and cell cycle distribution patterns.To elucidate the interconnections among various cellular responses,we employed a suite of statistical techniques encompassing correlation studies,principal component analysis(PCA)-based dimensionality reduction,and dendrogram-based clusteringmethodologies.Results:Thewater extract exhibited dosedependent cytotoxicity with IC50 values of 372.3±8.7μg/mL and 202.5±8.4μg/mL for B16BL6 and B16F10 cells,respectively.VCA showed more significant effects,with IC50 values of 0.1992±0.0041 and 0.1981±0.0098μg/mL for B16BL6 and B16F10 cells,respectively.Both agents induced substantial apoptosis with a significant progression from early to late apoptotic stages,reaching up to 59.4%total apoptotic cells for VCA treatment.This was confirmed by strong multicaspase activation,particularly in VCA-treated cells(up to 88.4%caspase-positive cells).The water extract showed modest effects on cell cycle distribution,with increases in G0/G1 phase(74.6%)in B16BL6 cells and S phase(19.2%)in B16F10 cells,while VCA treatment resulted in G2/M phase reduction(10.0%)in B16F10 cells.Correlation analysis revealed strong negative associations between cell viability and caspase activity(r=−0.843 to−0.878),while hierarchical clustering demonstrated distinct response patterns between low and high concentrations of both agents.Effect size analysis confirmed strong treatment impacts on cell viability(d=−5.89 to−6.12)and caspase activation(d=3.45 to 5.23).Conclusion:These findings suggest that Korean mistletoe water extract and its isolated lectin may affect both primary and metastatic melanoma cells through distinct mechanisms,demonstrating particular potency in caspase-dependent apoptosis induction.Our findings establish a robust foundation for developing novel therapeutic interventions derived from natural compounds to combat malignant melanoma with high metastatic potential.展开更多
The factors which effected the extraction of total flavones from Polygonum aviculare L. var. aviculare were discussed with respects to solvent, concentration and the extracting temperature. The rutin was used as a sta...The factors which effected the extraction of total flavones from Polygonum aviculare L. var. aviculare were discussed with respects to solvent, concentration and the extracting temperature. The rutin was used as a standard compound, the total flavones extracted by the different extracting procedure were quantitated by measurement of absorbance at 510 nm. The results show that 80 ℃ and 35% alcohol are optimal and the extracting rate of the total flavones is as high as 4.349%.展开更多
以蓝靛果(Lonicera caerulea L. var. edulis Jurcz et Herd)带芽茎段为外植体进行组培快繁,考察了激素种类和浓度对外植体增殖及不定芽生根的影响。结果表明,适合外植体增殖的培养基为MS+2.0mg/L6-BA+0.2 mg/L IBA,增殖系数达3.3,适合...以蓝靛果(Lonicera caerulea L. var. edulis Jurcz et Herd)带芽茎段为外植体进行组培快繁,考察了激素种类和浓度对外植体增殖及不定芽生根的影响。结果表明,适合外植体增殖的培养基为MS+2.0mg/L6-BA+0.2 mg/L IBA,增殖系数达3.3,适合不定芽生根的培养基为1/2MS+0.5 mg/L NAA+0.5 mg/L IBA,生根率达72%。展开更多
基金supported by the National Key Research and Development Program of China(2023YFF1104001).
文摘Insulin resistance(IR)is a central event in the pathogenesis of type 2 diabetes mellitus(T2DM).In recent years,the global burden of T2DM has intensified,with conventional therapeutic agents carrying certain side effects.Therefore,there is an urgent need to identify safer and more effective alternative therapies,which has accelerated research on natural green plants.Lonicera caerulea var.edulis is abundant in diverse bioactive constituents,however,the hypoglycemic potential of its important bioactive component—polysaccharides—has not yet been fully exploited.Within this investigation,a native polysaccharide(LP)was separated from L.caerulea var.edulis.Structural analysis revealed that LP was an acidic heteropolysaccharide possessing a molecular weight of 109.64 kDa,consisting mainly of galacturonic acid,galactose,and arabinose.Additionally,LP contained both HG and RG-I domains and showed a porous layered structure with favorable thermal stability.In insulin resistant HepG2(IR-HepG2)cell model,LP ameliorated insulin resistance,as evidenced by increased glucose consumption(up to 1.39-fold),glycogen synthesis(up to 2.34-fold),and enhanced activities of hexokinase(up to 1.48-fold)and pyruvate kinase(up to 1.98-fold)relative to the model group,through modulation of the IRS-1/PI3K/Akt signaling pathway.Notably,at concentrations of 100-200μg/mL,LP demonstrated significant hypoglycemic effects,markedly improving these metabolic parameters and thereby alleviating insulin resistance.These findings confirm LP's capability to regulate T2DM,establishing a scientific basis for the prospective use of L.caerulea var.edulis as a promising functional food resource.
文摘Objectives:Among cutaneous malignancies,melanoma stands out for its particularly aggressive nature,with therapeutic interventions becoming notably limited once the disease progresses.In this research,we investigate the tumor-suppressing capabilities of water-extracted Korean mistletoe(Viscum album var.coloratum)and its purified lectin component(V.album var.coloratum agglutinin,VCA)using two distinct mouse melanoma models:B16BL6 and B16F10 cell lines.Methods:The impact of water extract and VCA treatments on melanoma cells was assessed through multiple experimental approaches,examining cellular survival rates,programmed cell death pathways,multicaspase activity,and cell cycle distribution patterns.To elucidate the interconnections among various cellular responses,we employed a suite of statistical techniques encompassing correlation studies,principal component analysis(PCA)-based dimensionality reduction,and dendrogram-based clusteringmethodologies.Results:Thewater extract exhibited dosedependent cytotoxicity with IC50 values of 372.3±8.7μg/mL and 202.5±8.4μg/mL for B16BL6 and B16F10 cells,respectively.VCA showed more significant effects,with IC50 values of 0.1992±0.0041 and 0.1981±0.0098μg/mL for B16BL6 and B16F10 cells,respectively.Both agents induced substantial apoptosis with a significant progression from early to late apoptotic stages,reaching up to 59.4%total apoptotic cells for VCA treatment.This was confirmed by strong multicaspase activation,particularly in VCA-treated cells(up to 88.4%caspase-positive cells).The water extract showed modest effects on cell cycle distribution,with increases in G0/G1 phase(74.6%)in B16BL6 cells and S phase(19.2%)in B16F10 cells,while VCA treatment resulted in G2/M phase reduction(10.0%)in B16F10 cells.Correlation analysis revealed strong negative associations between cell viability and caspase activity(r=−0.843 to−0.878),while hierarchical clustering demonstrated distinct response patterns between low and high concentrations of both agents.Effect size analysis confirmed strong treatment impacts on cell viability(d=−5.89 to−6.12)and caspase activation(d=3.45 to 5.23).Conclusion:These findings suggest that Korean mistletoe water extract and its isolated lectin may affect both primary and metastatic melanoma cells through distinct mechanisms,demonstrating particular potency in caspase-dependent apoptosis induction.Our findings establish a robust foundation for developing novel therapeutic interventions derived from natural compounds to combat malignant melanoma with high metastatic potential.
文摘The factors which effected the extraction of total flavones from Polygonum aviculare L. var. aviculare were discussed with respects to solvent, concentration and the extracting temperature. The rutin was used as a standard compound, the total flavones extracted by the different extracting procedure were quantitated by measurement of absorbance at 510 nm. The results show that 80 ℃ and 35% alcohol are optimal and the extracting rate of the total flavones is as high as 4.349%.
文摘以蓝靛果(Lonicera caerulea L. var. edulis Jurcz et Herd)带芽茎段为外植体进行组培快繁,考察了激素种类和浓度对外植体增殖及不定芽生根的影响。结果表明,适合外植体增殖的培养基为MS+2.0mg/L6-BA+0.2 mg/L IBA,增殖系数达3.3,适合不定芽生根的培养基为1/2MS+0.5 mg/L NAA+0.5 mg/L IBA,生根率达72%。
