Objectives:Among cutaneous malignancies,melanoma stands out for its particularly aggressive nature,with therapeutic interventions becoming notably limited once the disease progresses.In this research,we investigate th...Objectives:Among cutaneous malignancies,melanoma stands out for its particularly aggressive nature,with therapeutic interventions becoming notably limited once the disease progresses.In this research,we investigate the tumor-suppressing capabilities of water-extracted Korean mistletoe(Viscum album var.coloratum)and its purified lectin component(V.album var.coloratum agglutinin,VCA)using two distinct mouse melanoma models:B16BL6 and B16F10 cell lines.Methods:The impact of water extract and VCA treatments on melanoma cells was assessed through multiple experimental approaches,examining cellular survival rates,programmed cell death pathways,multicaspase activity,and cell cycle distribution patterns.To elucidate the interconnections among various cellular responses,we employed a suite of statistical techniques encompassing correlation studies,principal component analysis(PCA)-based dimensionality reduction,and dendrogram-based clusteringmethodologies.Results:Thewater extract exhibited dosedependent cytotoxicity with IC50 values of 372.3±8.7μg/mL and 202.5±8.4μg/mL for B16BL6 and B16F10 cells,respectively.VCA showed more significant effects,with IC50 values of 0.1992±0.0041 and 0.1981±0.0098μg/mL for B16BL6 and B16F10 cells,respectively.Both agents induced substantial apoptosis with a significant progression from early to late apoptotic stages,reaching up to 59.4%total apoptotic cells for VCA treatment.This was confirmed by strong multicaspase activation,particularly in VCA-treated cells(up to 88.4%caspase-positive cells).The water extract showed modest effects on cell cycle distribution,with increases in G0/G1 phase(74.6%)in B16BL6 cells and S phase(19.2%)in B16F10 cells,while VCA treatment resulted in G2/M phase reduction(10.0%)in B16F10 cells.Correlation analysis revealed strong negative associations between cell viability and caspase activity(r=−0.843 to−0.878),while hierarchical clustering demonstrated distinct response patterns between low and high concentrations of both agents.Effect size analysis confirmed strong treatment impacts on cell viability(d=−5.89 to−6.12)and caspase activation(d=3.45 to 5.23).Conclusion:These findings suggest that Korean mistletoe water extract and its isolated lectin may affect both primary and metastatic melanoma cells through distinct mechanisms,demonstrating particular potency in caspase-dependent apoptosis induction.Our findings establish a robust foundation for developing novel therapeutic interventions derived from natural compounds to combat malignant melanoma with high metastatic potential.展开更多
分别以库拉索芦荟(Aloe barbadensis Mill.)和中华芦荟(Aloe vera L.var.Chinesis)老叶、中叶、嫩叶的整个叶片及叶片中间凝胶部分为样品,比较2种芦荟不同部位的抗氧化酶活性。结果表明,库拉索芦荟、中华芦荟不同部位抗氧化酶间活性差...分别以库拉索芦荟(Aloe barbadensis Mill.)和中华芦荟(Aloe vera L.var.Chinesis)老叶、中叶、嫩叶的整个叶片及叶片中间凝胶部分为样品,比较2种芦荟不同部位的抗氧化酶活性。结果表明,库拉索芦荟、中华芦荟不同部位抗氧化酶间活性差异较大。库拉索芦荟超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)活性最高部位分别是老叶中间凝胶、中叶整个叶片、老叶整个叶片;中华芦荟SOD、CAT、POD活性最高部位分别是嫩叶中间凝胶、老叶整个叶片、老叶整个叶片。结合生物学产量,开发应用SOD方面,应以2种芦荟中叶中间凝胶为原料;开发应用CAT和POD方面,应以2种芦荟老叶整个叶片为原料。展开更多
The factors which effected the extraction of total flavones from Polygonum aviculare L. var. aviculare were discussed with respects to solvent, concentration and the extracting temperature. The rutin was used as a sta...The factors which effected the extraction of total flavones from Polygonum aviculare L. var. aviculare were discussed with respects to solvent, concentration and the extracting temperature. The rutin was used as a standard compound, the total flavones extracted by the different extracting procedure were quantitated by measurement of absorbance at 510 nm. The results show that 80 ℃ and 35% alcohol are optimal and the extracting rate of the total flavones is as high as 4.349%.展开更多
文摘Objectives:Among cutaneous malignancies,melanoma stands out for its particularly aggressive nature,with therapeutic interventions becoming notably limited once the disease progresses.In this research,we investigate the tumor-suppressing capabilities of water-extracted Korean mistletoe(Viscum album var.coloratum)and its purified lectin component(V.album var.coloratum agglutinin,VCA)using two distinct mouse melanoma models:B16BL6 and B16F10 cell lines.Methods:The impact of water extract and VCA treatments on melanoma cells was assessed through multiple experimental approaches,examining cellular survival rates,programmed cell death pathways,multicaspase activity,and cell cycle distribution patterns.To elucidate the interconnections among various cellular responses,we employed a suite of statistical techniques encompassing correlation studies,principal component analysis(PCA)-based dimensionality reduction,and dendrogram-based clusteringmethodologies.Results:Thewater extract exhibited dosedependent cytotoxicity with IC50 values of 372.3±8.7μg/mL and 202.5±8.4μg/mL for B16BL6 and B16F10 cells,respectively.VCA showed more significant effects,with IC50 values of 0.1992±0.0041 and 0.1981±0.0098μg/mL for B16BL6 and B16F10 cells,respectively.Both agents induced substantial apoptosis with a significant progression from early to late apoptotic stages,reaching up to 59.4%total apoptotic cells for VCA treatment.This was confirmed by strong multicaspase activation,particularly in VCA-treated cells(up to 88.4%caspase-positive cells).The water extract showed modest effects on cell cycle distribution,with increases in G0/G1 phase(74.6%)in B16BL6 cells and S phase(19.2%)in B16F10 cells,while VCA treatment resulted in G2/M phase reduction(10.0%)in B16F10 cells.Correlation analysis revealed strong negative associations between cell viability and caspase activity(r=−0.843 to−0.878),while hierarchical clustering demonstrated distinct response patterns between low and high concentrations of both agents.Effect size analysis confirmed strong treatment impacts on cell viability(d=−5.89 to−6.12)and caspase activation(d=3.45 to 5.23).Conclusion:These findings suggest that Korean mistletoe water extract and its isolated lectin may affect both primary and metastatic melanoma cells through distinct mechanisms,demonstrating particular potency in caspase-dependent apoptosis induction.Our findings establish a robust foundation for developing novel therapeutic interventions derived from natural compounds to combat malignant melanoma with high metastatic potential.
文摘分别以库拉索芦荟(Aloe barbadensis Mill.)和中华芦荟(Aloe vera L.var.Chinesis)老叶、中叶、嫩叶的整个叶片及叶片中间凝胶部分为样品,比较2种芦荟不同部位的抗氧化酶活性。结果表明,库拉索芦荟、中华芦荟不同部位抗氧化酶间活性差异较大。库拉索芦荟超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)活性最高部位分别是老叶中间凝胶、中叶整个叶片、老叶整个叶片;中华芦荟SOD、CAT、POD活性最高部位分别是嫩叶中间凝胶、老叶整个叶片、老叶整个叶片。结合生物学产量,开发应用SOD方面,应以2种芦荟中叶中间凝胶为原料;开发应用CAT和POD方面,应以2种芦荟老叶整个叶片为原料。
文摘The factors which effected the extraction of total flavones from Polygonum aviculare L. var. aviculare were discussed with respects to solvent, concentration and the extracting temperature. The rutin was used as a standard compound, the total flavones extracted by the different extracting procedure were quantitated by measurement of absorbance at 510 nm. The results show that 80 ℃ and 35% alcohol are optimal and the extracting rate of the total flavones is as high as 4.349%.
