Objective:Prunella vulgaris L.has long been used for liver protection according to traditional Chinese medicine theory and has been proven by modern pharmacological research to have multiple potential liver-protective...Objective:Prunella vulgaris L.has long been used for liver protection according to traditional Chinese medicine theory and has been proven by modern pharmacological research to have multiple potential liver-protective effects.However,its effects on non-alcoholic steatohepatitis(NASH)are currently uncertain.Our study explores the effects of P.vulgaris polysaccharides on NASH and intestinal homeostasis.Methods:An aqueous extract of the dried fruit spikes of P.vulgaris was precipitated in an 85%ethanol solution(PVE85)to extract crude polysaccharides from the herb.A choline-deficient,L-amino aciddefined,high-fat diet(CDAHFD)was administrated to male C57BL/6 mice to establish a NASH animal model.After 4 weeks,the PVE85 group was orally administered PVE85(200 mg/[kg·d]),while the control group and CDAHFD group were orally administered vehicle for 6 weeks.Quantitative real-time polymerase chain reaction analysis,Western blotting,immunohistochemistry and other methods were used to assess the impact of PVE85 on the liver in mice with NASH.16S rRNA gene amplicon analysis was employed to evaluate the gut microbiota abundance and diversity in each group to examine alterations at various taxonomic levels.Results:PVE85 significantly reversed the course of NASH in mice.mRNA levels of inflammatory mediators associated with NASH and protein expression of hepatic nucleotide-binding leucine-rich repeat and pyrin domain-containing protein 3(NLRP3)were significantly reduced after PVE85 treatment.Moreover,PVE85 attenuated the thickening and cross-linking of collagen fibres and inhibited the expression of fibrosis-related mRNAs in the livers of NASH mice.Intriguingly,PVE85 restored changes in the gut microbiota and improved intestinal barrier dysfunction induced by NASH by increasing the abundance of Actinobacteria and reducing the abundance of Proteobacteria at the phylum level.PVE85 had significant activity in reducing the relative abundance of Clostridiaceae at the family levels.PVE85 markedly enhanced the abundance of some beneficial micro-organisms at various taxonomic levels as well.Additionally,the physicochemical environment of the intestine was effectively improved,involving an increase in the density of intestinal villi,normalization of the intestinal pH,and improvement of intestinal permeability.Conclusion:PVE85 can reduce hepatic lipid overaccumulation,inflammation,and fibrosis in an animal model of CDAHFD-induced NASH and improve the intestinal microbial composition and intestinal structure.展开更多
[Objective] This experiment was conducted to investigate the optimal ex- traction process and antioxidant activity of polysaccharide in Sagittaria sagittifo/ia L. [Method] The effects of extraction temperature, extrac...[Objective] This experiment was conducted to investigate the optimal ex- traction process and antioxidant activity of polysaccharide in Sagittaria sagittifo/ia L. [Method] The effects of extraction temperature, extraction time, extraction times and material-to-liquid ratio on content of polysaccharides in S. sagittifo/ia were investi- gated. On the basis of single-factor tests, an orthogonal test was carried out to de- termine the optimal process parameters. In addition, the in-vitro antioxidant activity of S. sagittifo/ia polysaccharides was evaluated on the basis of determination of their scavenging activities against DPPH and hydroxyl free radicals and reducing power. [Result] The results showed that the optimized process parameters were as follows： material-to-liquid ratio at 1：40 （g/ml）, extraction temperature at 90 ℃, extrac- tion time of 4 h, and extraction times of 3 times. Under this condition, the content of S. sagittifo/ia polysaccharides was 29.32%. For 1 mg/ml S. sagittifo/ia polysaccha- rides, the scavenging ratios of DPPH and hydroxyl free radicals were 70.62% and 35.82%, respectively, and in the determination of reducing power, the absorbance at 700 nm was 0.453 1. [Conclusion] It could be concluded that S. sagittifolia polysac- charides have strong antioxidant capacity to have free radicals scavenged in vitro.展开更多
基金supported by the Launch fee for talent introduction and research at Shanghai University of Traditional Chinese Medicine(A1-U23-205-020411)the Guangdong Province Key Area R&D Program of China(2020B1111110003)。
文摘Objective:Prunella vulgaris L.has long been used for liver protection according to traditional Chinese medicine theory and has been proven by modern pharmacological research to have multiple potential liver-protective effects.However,its effects on non-alcoholic steatohepatitis(NASH)are currently uncertain.Our study explores the effects of P.vulgaris polysaccharides on NASH and intestinal homeostasis.Methods:An aqueous extract of the dried fruit spikes of P.vulgaris was precipitated in an 85%ethanol solution(PVE85)to extract crude polysaccharides from the herb.A choline-deficient,L-amino aciddefined,high-fat diet(CDAHFD)was administrated to male C57BL/6 mice to establish a NASH animal model.After 4 weeks,the PVE85 group was orally administered PVE85(200 mg/[kg·d]),while the control group and CDAHFD group were orally administered vehicle for 6 weeks.Quantitative real-time polymerase chain reaction analysis,Western blotting,immunohistochemistry and other methods were used to assess the impact of PVE85 on the liver in mice with NASH.16S rRNA gene amplicon analysis was employed to evaluate the gut microbiota abundance and diversity in each group to examine alterations at various taxonomic levels.Results:PVE85 significantly reversed the course of NASH in mice.mRNA levels of inflammatory mediators associated with NASH and protein expression of hepatic nucleotide-binding leucine-rich repeat and pyrin domain-containing protein 3(NLRP3)were significantly reduced after PVE85 treatment.Moreover,PVE85 attenuated the thickening and cross-linking of collagen fibres and inhibited the expression of fibrosis-related mRNAs in the livers of NASH mice.Intriguingly,PVE85 restored changes in the gut microbiota and improved intestinal barrier dysfunction induced by NASH by increasing the abundance of Actinobacteria and reducing the abundance of Proteobacteria at the phylum level.PVE85 had significant activity in reducing the relative abundance of Clostridiaceae at the family levels.PVE85 markedly enhanced the abundance of some beneficial micro-organisms at various taxonomic levels as well.Additionally,the physicochemical environment of the intestine was effectively improved,involving an increase in the density of intestinal villi,normalization of the intestinal pH,and improvement of intestinal permeability.Conclusion:PVE85 can reduce hepatic lipid overaccumulation,inflammation,and fibrosis in an animal model of CDAHFD-induced NASH and improve the intestinal microbial composition and intestinal structure.
基金Supported by Scientific Research Project of Education Department of Sichuan Province(16ZB0199)~~
文摘[Objective] This experiment was conducted to investigate the optimal ex- traction process and antioxidant activity of polysaccharide in Sagittaria sagittifo/ia L. [Method] The effects of extraction temperature, extraction time, extraction times and material-to-liquid ratio on content of polysaccharides in S. sagittifo/ia were investi- gated. On the basis of single-factor tests, an orthogonal test was carried out to de- termine the optimal process parameters. In addition, the in-vitro antioxidant activity of S. sagittifo/ia polysaccharides was evaluated on the basis of determination of their scavenging activities against DPPH and hydroxyl free radicals and reducing power. [Result] The results showed that the optimized process parameters were as follows： material-to-liquid ratio at 1：40 （g/ml）, extraction temperature at 90 ℃, extrac- tion time of 4 h, and extraction times of 3 times. Under this condition, the content of S. sagittifo/ia polysaccharides was 29.32%. For 1 mg/ml S. sagittifo/ia polysaccha- rides, the scavenging ratios of DPPH and hydroxyl free radicals were 70.62% and 35.82%, respectively, and in the determination of reducing power, the absorbance at 700 nm was 0.453 1. [Conclusion] It could be concluded that S. sagittifolia polysac- charides have strong antioxidant capacity to have free radicals scavenged in vitro.
