Objective:To determine the anticandidal activities of Salvia officinalis L.(S.officinalis)essential oil against Candida albicans(C.albicans)and the inhibitory effects on the adhesion of C.albicans to polymethyl methac...Objective:To determine the anticandidal activities of Salvia officinalis L.(S.officinalis)essential oil against Candida albicans(C.albicans)and the inhibitory effects on the adhesion of C.albicans to polymethyl methacrylate(PMMA)resin surface.Methods:Disc diffusion method was first used to test the anticandidal activities of the S.officinalis L.essential oil against the reference strain(ATCC 90028)and 2 clinical strains of C.albicans.Then the minimal inhibitory concentration(MIC)and minimal lethal concentration(MLX)were determined by modified membrane method.The adhesion of C.albicans to PMMA resin surface was assessed after immersion with S.officinalis L.essential oil at various concentrations of 1XMIC.0.5XMIC and 0.25XMIC at room temperature for30 min.One-way ANOVA was used to compare the Candida cell adhesion with the pretreatment agents and Tukey's test was used for multiple comparisons.Results:5.officinalis L.essential oil exhibited anticandidal activity against all strains of C.albicans with inhibition zone ranging from 40.5 mm to 19.5 mm.The MIC and MLC of the oil were determined as 2.780 g/L against all test strains.According to the effects on C.albicans adhesion to PMMA resin surface,it was found that immersion in the essential oil at concentrations of 1XMIC(2.780 g/L).0.5XMIC(1.390 g/L)and0.25XMIC(0.695 g/L)for 30 min significantly reduced the adhesion of all 3 test strains to PMMA resin surface in a dose dependent manner(P<0.05).Conclusions:S.officinalis L.essential oil exhibited anticandidal activities against C.albicans and had inhibitory effects on the adhesion of the cells to PMMA resin surface.With further testing and development,S.officinalis essential oil may be used as an antifungal denture cleanser to prevent candidal adhesion and thus reduce the risk of candida-associated denture stomatitis.展开更多
The growing demand for natural bioactive compounds has renewed the interest in optimizing essential oil extraction methods from aromatic plants.In this study,two extraction techniques for the recovery of essential oil...The growing demand for natural bioactive compounds has renewed the interest in optimizing essential oil extraction methods from aromatic plants.In this study,two extraction techniques for the recovery of essential oils from Mentha spicata L.leaves,Microwave-Assisted Hydrodistillation(MAHD)and Steam Distillation(SD),were compared,focusing on yield,chemical profile,antioxidant,and antifungal activity.Both methods achieved comparable yields(MAHD:0.24%,SD:0.28%),but MAHD significantly reduced extraction time and energy consumption.GC-MS and ATR-FTIR analyses revealed a higher content of oxygenated monoterpenoids in MAHD oils(e.g.,carvone,32.60%),while SD oils were richer in hydrocarbons(e.g.,limonene,52.09%).MAHD extracts showed superior antioxidant and antifungal properties,especially against Saccharomyces cerevisiae and Kluyveromyces marxianus,probably due to the preservation of thermolabile and bioactive compounds.These results highlight MAHD as a sustainable and efficient technique for obtaining bioactive-rich essential oils with potential applications in the food,pharmaceutical,and cosmetic sectors.The choice of extraction method should be aligned with specific application goals,balancing yield,composition,and environmental impact.展开更多
基金Supported by Maxillofacial Prosthetic Service Research Fund,Faculty of Dentistry,Mahidol University,Bangkok,Thailand(Grant No.496/2011)
文摘Objective:To determine the anticandidal activities of Salvia officinalis L.(S.officinalis)essential oil against Candida albicans(C.albicans)and the inhibitory effects on the adhesion of C.albicans to polymethyl methacrylate(PMMA)resin surface.Methods:Disc diffusion method was first used to test the anticandidal activities of the S.officinalis L.essential oil against the reference strain(ATCC 90028)and 2 clinical strains of C.albicans.Then the minimal inhibitory concentration(MIC)and minimal lethal concentration(MLX)were determined by modified membrane method.The adhesion of C.albicans to PMMA resin surface was assessed after immersion with S.officinalis L.essential oil at various concentrations of 1XMIC.0.5XMIC and 0.25XMIC at room temperature for30 min.One-way ANOVA was used to compare the Candida cell adhesion with the pretreatment agents and Tukey's test was used for multiple comparisons.Results:5.officinalis L.essential oil exhibited anticandidal activity against all strains of C.albicans with inhibition zone ranging from 40.5 mm to 19.5 mm.The MIC and MLC of the oil were determined as 2.780 g/L against all test strains.According to the effects on C.albicans adhesion to PMMA resin surface,it was found that immersion in the essential oil at concentrations of 1XMIC(2.780 g/L).0.5XMIC(1.390 g/L)and0.25XMIC(0.695 g/L)for 30 min significantly reduced the adhesion of all 3 test strains to PMMA resin surface in a dose dependent manner(P<0.05).Conclusions:S.officinalis L.essential oil exhibited anticandidal activities against C.albicans and had inhibitory effects on the adhesion of the cells to PMMA resin surface.With further testing and development,S.officinalis essential oil may be used as an antifungal denture cleanser to prevent candidal adhesion and thus reduce the risk of candida-associated denture stomatitis.
基金supported by the project“IRPINIA ESSENZIALEPSR CAMPANIA 2014/2020.MISURA 19(DTA.AD002.674)"GAL IRPINIA,Misura 16“Cooperazione”Tipologia di Intervento16.1.1“Sostegno per la costituzione e il funzionamento dei GO del PEI in materia di produttivita e sostenibilita dell’agricoltura”-Azione 2“Sostegno ai Progetti Operativi di Innovazione(POI)”and in part by the CNR NUTRAGE FOE-2021 DBAAD005.225 project and by the CNR FUNZIONAMENTO ISA grant(DBA.AD005.178-CUP B32F11000540005).
文摘The growing demand for natural bioactive compounds has renewed the interest in optimizing essential oil extraction methods from aromatic plants.In this study,two extraction techniques for the recovery of essential oils from Mentha spicata L.leaves,Microwave-Assisted Hydrodistillation(MAHD)and Steam Distillation(SD),were compared,focusing on yield,chemical profile,antioxidant,and antifungal activity.Both methods achieved comparable yields(MAHD:0.24%,SD:0.28%),but MAHD significantly reduced extraction time and energy consumption.GC-MS and ATR-FTIR analyses revealed a higher content of oxygenated monoterpenoids in MAHD oils(e.g.,carvone,32.60%),while SD oils were richer in hydrocarbons(e.g.,limonene,52.09%).MAHD extracts showed superior antioxidant and antifungal properties,especially against Saccharomyces cerevisiae and Kluyveromyces marxianus,probably due to the preservation of thermolabile and bioactive compounds.These results highlight MAHD as a sustainable and efficient technique for obtaining bioactive-rich essential oils with potential applications in the food,pharmaceutical,and cosmetic sectors.The choice of extraction method should be aligned with specific application goals,balancing yield,composition,and environmental impact.
