[Objectives]To compare the leaf morphology and leaf venation patterns of Hedyotis auricularia L.and its adulterants,such as Mitracarpus hirtus(L.)DC.,Spermacoce pusilla Wall.and Spermacoce alata Aubl.,produced in Guan...[Objectives]To compare the leaf morphology and leaf venation patterns of Hedyotis auricularia L.and its adulterants,such as Mitracarpus hirtus(L.)DC.,Spermacoce pusilla Wall.and Spermacoce alata Aubl.,produced in Guangxi,so as to provide a simple and rapid identification method for the identification of H.auricularia L.in Guangxi.[Methods]LMVP(leaf morphology-venation pattern)identification method was used to study the characteristics of leaf morphology-venation pattern.[Results]Characteristics of leaf morphology-venation pattern:(i)H.auricularia L.:circular knotted-curved pinnate leaf venation without reaching the margin;the primary veins run straight without branches,the number of secondary veins is 5 to 9,and the angles included in tertiary veins are mostly near right angles or obtuse angles.(ii)M.hirtus(L.)DC.:curved pinnate leaf venation without reaching the margin;the primary veins run straight without obvious changes,the number of secondary veins is 3 to 5,and the tertiary veins are slender,and the included angles are mostly acute.(iii)S.pusilla Wall.:curved leaf venation without reaching the leaf margin;there are 4 to 5 pairs of secondary veins,most of which are opposite;the angle between the secondary veins and the primary veins in the middle and near the petiole is mostly medium acute angle,and the extension of the tertiary veins has no fixed direction.(iv)S.alata Aubl.:circular knotted-curved pinnate leaf venation;the primary veins are convex and straight without branches,and there are 4 to 6 pairs of secondary veins,alternating.The angle between secondary veins and primary veins is mostly medium acute angle,the angle between tertiary veins and secondary veins is near right angle,and the tertiary veins are mostly transversely extended.[Conclusions]The leaf morphology-venation patterns of the above-mentioned H.auricularia L.and M.hirtus(L.)DC.,S.pusilla Wall.,and S.alata Aubl.can be used as their identification features,and the identification method has certain operability,which provides an identification idea for the identification of Chinese herbal medicines.展开更多
We quantitatively determined four nucleosides, including cytidine, uridine, guanosine, and adenosine, in Carthamus tinctorius L. and Safflower injection. Separation was performed on a Zorbax Eclipse XDB-18 column usin...We quantitatively determined four nucleosides, including cytidine, uridine, guanosine, and adenosine, in Carthamus tinctorius L. and Safflower injection. Separation was performed on a Zorbax Eclipse XDB-18 column using a gradient elution with mobile phases of 0.05% trifluoroacetic acid （TFA） aqueous solution （A） and methanol （B）. The assay was carried out at a flow rate of 1 mL/min at 25 ℃ with detection at 260 nm. Cytidine, uridine, adenosine and guanosine showed good linearity in the ranges of4.02-503μg/mL （r2= 0.9998）, 9.38-1407 μg/mL （rz = 0.9999）, 80.6-8060μg/mL （r2 = 0.9999） and 2.10---630μg/mL （r2 = 0.9987） with average recoveries of 97.2%, 94.5%, 98.6% and 108.6%, respectively. The contents of cytidine, uridine, adenosine and guanosine in different Carthamus tinctorius L. and Safflower injection were significantly different. This is the first report on the quantitative determination of nucleosides in Carthamus tinctorius L. and Safflower injection.展开更多
Some wild species of Oryza possess agronomi-cally useful traits such as disease/insect resis-tance and salt tolerance.However,the use ofwild Oryza species for rice improvement hasbeen hampered by the sexual incompatib...Some wild species of Oryza possess agronomi-cally useful traits such as disease/insect resis-tance and salt tolerance.However,the use ofwild Oryza species for rice improvement hasbeen hampered by the sexual incompatibility orsterility of hybrids obtained by conventionalmethods.We have used protoplast fusion as amethod to incorporate useful trait of wild O-ryza species into cultivated rice and~ obtainedsomatic hybrid plants between O.sativa and展开更多
Male sterile NEA plants were identified in progenies of the radiated 92P x Aggregate-silique in Brassica napus L. in 1993. Their progeny plants from test crossing and open pollination were 100% male sterile. The doubl...Male sterile NEA plants were identified in progenies of the radiated 92P x Aggregate-silique in Brassica napus L. in 1993. Their progeny plants from test crossing and open pollination were 100% male sterile. The double-low male sterile lines JL-4 and JL-18 were bred through successive backcrossing of the double -low variety No.4 and No. 18 in Brassica napus L.to NEA. Restorer lines 6720 and 6722 with significant heterosis in F1 were developed. The results from investigating the restoring-ntaintaining relationship and inheritance of the restorer gene indicated that JL-4 and JL-18 were different from both PolCMS and Shan 2A type, and their restorer gene was controlled by a pair of dominant genie genes.展开更多
In order to utilize the wasted saline-sodic soils under shallow groundwater condition, a 3-year field study was carried in a field cropped with Lycium barbarum L. and irrigated by drip irrigation with saline groundwat...In order to utilize the wasted saline-sodic soils under shallow groundwater condition, a 3-year field study was carried in a field cropped with Lycium barbarum L. and irrigated by drip irrigation with saline groundwater under the water table depth of 30-40 cm in the northern Yinchuan Plain, China. Effects of cropping duration （one, two, and three years） on soil salinity, soil solution composition, and pH in three adjacent plots were investigated in 2008. Results showed that a high irrigation frequency maintained high soil water potential and subsequently facilitated infiltration and downward movement of water and salt in the crop root zone. Salt accumulated on the edges of the ridges, and soil saturated-paste electrical conductivity （ECe） was higher in the edge. Concentrations of Na^＋, Ca^2＋, Mg^2＋, Cl^-, and SO24^- in the soil increased with the soil depth as did the ECe, while HCO3 and pH had a relative uniform distribution in soil profile. As planting year increased, the ECe and soil salts in the field had a decreasing tendency, while in the root zone they decreased immediately after irrigation and then remained relatively stable in the following growing seasons. HCO3 and pH had little change with the planting year. Results suggested that the application of drip irrigation with saline water could ameliorate saline-sodic soil and provide a relatively feasible soil environment for saline-sodic soils with shallow groundwater.展开更多
A new isochroman-4-one, 7,8-dihydroxy-3-methylisochroman-4-one was isolated from water soluble fraction of Musa sapientum L. Its structure was determined by spectroscopic evidences and its total synthesis has also bee...A new isochroman-4-one, 7,8-dihydroxy-3-methylisochroman-4-one was isolated from water soluble fraction of Musa sapientum L. Its structure was determined by spectroscopic evidences and its total synthesis has also been reported. The compound showed potent antihypertensive activity.展开更多
An undescribed pyrrole acid,1-(4′-methoxy-4′-oxobutyl)-1 H-pyrrole-2,5-dicarboxylic acid(1)and one known pyr-role acid(2)were isolated from the fruits of Phyllanthus emblica.The structures of these compounds were el...An undescribed pyrrole acid,1-(4′-methoxy-4′-oxobutyl)-1 H-pyrrole-2,5-dicarboxylic acid(1)and one known pyr-role acid(2)were isolated from the fruits of Phyllanthus emblica.The structures of these compounds were elucidated via the comprehensive analyses of IR,HRESIMS,1D and 2D spectroscopic data.A series of biological assays revealed that compounds 1 and 2 could inhibit LPS-induced over-production of nitric oxide(NO),interleukin-6(IL-6),monocyte chemotactic protein 1(MCP-1)and tumor necrosis factor-α(TNF-α)by reducing the phosphorylation of extracellular regulated protein kinases(ERK)and c-Jun N-terminal kinases(JNK)in RAW 264.7 cells.Additionally,compounds 1 and 2 were found to reduce lipid deposition and increase the mRNA expression of ATP-binding cassette transporter A1 in oxidized low-density lipoprotein-treated RAW264.7 macrophages.展开更多
In this study,the fibers of invasive species Agave americana L.and Ricinus communis L.were successfully used for the first time as new sources to produce cytocompatible and highly crystalline cellulose nanofibers.Cell...In this study,the fibers of invasive species Agave americana L.and Ricinus communis L.were successfully used for the first time as new sources to produce cytocompatible and highly crystalline cellulose nanofibers.Cellulose nanofibers were obtained by two methods,based on either alkaline or acid hydrolysis.The morphology,chemical composition,and crystallinity of the obtained materials were characterized by scanning electron microscopy(SEM)together with energy-dispersive X-ray spectroscopy(EDX),dynamic light scattering(DLS),X-ray diffraction(XRD),and Fourier transform infrared(FTIR)spectroscopy.The crystallinity indexes(CIs)of the cellulose nanofibers extracted from A.americana and R.communis were very high(94.1%and 92.7%,respectively).Biological studies evaluating the cytotoxic effects of the prepared cellulose nanofibers on human embryonic kidney 293 T(HEK293 T)cells were also performed.The nanofibers obtained using the two different extraction methods were all shown to be cytocompatible in the concentration range assayed(i.e.,0-500μg/mL).Our results showed that the nanocellulose extracted from A.americana and R.communis fibers has high potential as a new renewable green source of highly crystalline cellulose-based cytocompatible nanomaterials for biomedical applications.展开更多
The effects of different electrode on Solanum nigrum L.seed germination were determined.The result showed that germination percentage(GP)of seeds in treatment T2(titanium elec-trode)was 26.6%higher than in control(CK,...The effects of different electrode on Solanum nigrum L.seed germination were determined.The result showed that germination percentage(GP)of seeds in treatment T2(titanium elec-trode)was 26.6%higher than in control(CK,without electric field).High potassium and calcium concentrations were beneficial for seed enzymatic activity in treatment T2,which could partly explain the increase in GP.Cd accumulation(μg/pot)in S.nigrum treated with any electric field was significantly higher(p<0.05)than in CK without electric field.Specif-ically,Cd accumulation under the treatment T3(stainless steel electrode)was the highest both in roots and shoots;this accumulation in shoots and roots were 74.7%and 67.4%higher for stainless steel than in CK.This increase must have been associated with a higher Cd concentration in plants and did not exert a significant effect on the biomass.In par-ticular,Cd concentrations in roots and shoots under stainless steel treatment were both significantly higher than in CK(p<0.05),which had to be related to the higher available Cd concentration in the soil in the middle region.Furthermore,it could be attributed to al-tered soil pH and other soil properties.Moreover,none of the biomasses were significantly affected(p<0.05)by different electrode materials compared to CK.展开更多
The effects of ethanol extract of Bidens pilosa L.(EEB)on acute exercise fatigue and its underlying biochemical mechanism were investigated in this study.Sixty adult male ICR mice were divided into control,model,vitam...The effects of ethanol extract of Bidens pilosa L.(EEB)on acute exercise fatigue and its underlying biochemical mechanism were investigated in this study.Sixty adult male ICR mice were divided into control,model,vitamin C(VC)100,EEB40,EEB80,and EEB160 groups,receiving VC(100 mg/kg)or EEB(40,80,160 mg/kg)for 28 days(intragastrically,I.G.).The mice underwent tailsuspension,elevated plus maze(EPM),rotarod,and loaded swimming tasks and biochemical indices were measured.There were no significant differences in body weight,tail suspension time,EPM open arm time/entries and serum cortisone levels among the groups.Compared with the model group,there was an increase in rotarod latency in the VC100/EEB80 groups and an increase in loaded swimming time in the EEB80/EEB160 groups.Furthermore,the haptic and muscle glycogen levels decreased in the model group,while the haptic glycogen levels increased in the all VC/EEB groups.Similarly,the serum lactate and creatinine levels increased in the model group,but decreased in lactate(except for EEB160),creatinine(except for EEB40)and lactate dehydrogenase in the EEB80 group.In the liver,malonaldehyde(MDA)and oxidized glutathione(GSSG)levels increased in the model group;however,glutathione reductase(GR)(except for EEB40),glutathione(GSH)and GSH/GSSG ratios increased,with GSSG levels decreasing in all VC/EEB groups.In the quadriceps,the GR levels increased in the model,whereas it decreased in the VC100,EEB40 and EEB80 groups.These results suggest that EEB has anti-acute fatigue effect,potentially attributed to mitigate metabolite accumulation,enhancing glycogen reserves,and fortifying the antioxidant mechanism.展开更多
Solanum nigrum L.,belonging to the genus Solanum of Solanaceae family,is widely distributed in temperate and tropical regions of the world.It possesses the effects of clearing away heat and detoxification.A wide spect...Solanum nigrum L.,belonging to the genus Solanum of Solanaceae family,is widely distributed in temperate and tropical regions of the world.It possesses the effects of clearing away heat and detoxification.A wide spectrum of chemical constituents have been isolated from the Solanum nigrum L.,including lignans,steroids,etc.The structures of the eight compounds were determined by comprehensive spectroscopic analyses.It is noteworthy that compounds 1–4,6 and 8 have been isolated from Solanum nigrum L.for the first time.All compounds were evaluated for their antioxidant activities by ABTS and DPPH assays.Based on the results,Solanum nigrum L.could be used as a new natural antioxidant in biomedical applications.展开更多
Normal-phase and reversed-phase silica gel column chromatography and semi-preparative high performance liquid chromatography were applied to study the chemical constituents of the stems and twigs of the Hainan mangrov...Normal-phase and reversed-phase silica gel column chromatography and semi-preparative high performance liquid chromatography were applied to study the chemical constituents of the stems and twigs of the Hainan mangrove plant,Excoecaria agallocha L.A new 3,4-seco-ent-kaurane diterpenoid,named agallochanin acid A(1),and a rare ent-tetranorlabdane diterpenoid,named agallochanin acid B(2),along with four known diterpenoids were isolated.Their structures including absolute configurations were characterized via HR-ESI-MS,extensive 1D and 2D NMR investigations and calculated and experimental electronic circular dichroism(ECD)data.All isolates were tested for their inhibitory effects on NO production in lipopolysaccharide(LPS)-activated BV-2 cells.ent-3,4-Seco-17-oxo-kaur-4(19),15(16)-dien-3-oic acid(3)and ent-15-hydroxy-labda-8(17),13E-dien-3-one(5)exhibited considerable anti-neuroinflammatory activities with IC50 values of(14.32±1.99)and(4.24±0.37)μmol/L.展开更多
[Objectives]To make pharmacognostic identification of Euphorbia maculata L.and its related species.[Methods]The classical pharmacognostic identification method was adopted.[Results]The four main medicinal materials ar...[Objectives]To make pharmacognostic identification of Euphorbia maculata L.and its related species.[Methods]The classical pharmacognostic identification method was adopted.[Results]The four main medicinal materials are very similar,the fluff can be seen in E.maculata and Euphorbia thymifolia L.,but not in Euphorbia prostrata Ait.and Euphorbia taihsiensis(Chaw et Koutnik)Oudejians;the tissue structure is basically the same;except for E.taihsiensis without non-glandular hairs,the powder has secretory cells,laticifers,cells,calcium oxalate crystals,fibers,vessels,and seed coat fragments.Through ultrasonic extraction with 80%ethanol,extraction with isobutanol,extending with chloroform∶ethyl acetate∶formic acid(5∶3∶0.3),developing color with 3%aluminum trichloride ethanol solution,under ultraviolet light(365 nm),the fluorescent spots of the same color appeared on the corresponding position of the chromatogram of the reference substance(quercetin,kaempferol).[Conclusions]It is not easy to distinguish the four medicinal materials by character identification and microscopic identification,while the thin-layer chromatography(TLC)is more reliable.展开更多
Botanical morphological characteristics of Physalis alkekengi L.is first introduced,and its medicinal and edible value is elaborated.Cultivation techniques of P.alkekengi L.are mainly introduced.The research aims to p...Botanical morphological characteristics of Physalis alkekengi L.is first introduced,and its medicinal and edible value is elaborated.Cultivation techniques of P.alkekengi L.are mainly introduced.The research aims to provide a certain basis for the better development and application of P.alkekengi L.展开更多
On the basis of research on the origin of Mongolian medicine Veratrum nigrum L.,this paper summarizes the research situation of Mongolian medicine Veratrum nigrum L.from the aspects of clinical application,chemical co...On the basis of research on the origin of Mongolian medicine Veratrum nigrum L.,this paper summarizes the research situation of Mongolian medicine Veratrum nigrum L.from the aspects of clinical application,chemical composition,pharmacological action and toxicological action,so as to provide a reference basis for further research and further development and utilization of Mongolian medicine.展开更多
This paper introduces the biological characteristics,medicinal value,chemical component,and pharmacological effects of Crocus sativus L.,and explores its therapeutic effects on cardiovascular and cerebrovascular disea...This paper introduces the biological characteristics,medicinal value,chemical component,and pharmacological effects of Crocus sativus L.,and explores its therapeutic effects on cardiovascular and cerebrovascular diseases such as angina pectoris and coronary heart disease.展开更多
Sophora alopecuroides L.,a perennial plant belonging to Leguminosae family,is used as medicinal materials to treat a variety of skin diseases.Its chemical components are complex and diverse,mainly including alkaloids ...Sophora alopecuroides L.,a perennial plant belonging to Leguminosae family,is used as medicinal materials to treat a variety of skin diseases.Its chemical components are complex and diverse,mainly including alkaloids and flavonoids.Modern pharmacological studies have found that the isolated compounds from S.alopecuroides have a variety of pharmacological activities.In particular,alkaloids represented by matrine,aloperine and oxymatrine have significant anti-inflammatory,anti-viral,anti-tumor,anti-bacterial and immunomodulatory activities.This paper summarized the chemical constituents and pharmacological activities of S.alopecuroides,aiming to provide the basis for further research.展开更多
Parkinson’s disease(PD)is a common neurodegenerative disease.Despite a great deal of research on this disease,there is still no definitive treatment plan.Literature investigations have shown that Piper nigrum L.has a...Parkinson’s disease(PD)is a common neurodegenerative disease.Despite a great deal of research on this disease,there is still no definitive treatment plan.Literature investigations have shown that Piper nigrum L.has a protective effect on the nerve center.Therefore,network pharmacology was used in this study to investigate the therapeutic potential of P.nigrum for PD.As a result,six active alkaloid ingredients and four potential targets were obtained.This study laid the foundation for finding the lead compounds from P.nigrum to treat PD.展开更多
The essential oils from Mentha viridis (L). L. and Mentha pulegium L. were studied to assess their inhibitory potential on phospholipase from snake venoms and to determine their cytogenotoxic action on human cells. Th...The essential oils from Mentha viridis (L). L. and Mentha pulegium L. were studied to assess their inhibitory potential on phospholipase from snake venoms and to determine their cytogenotoxic action on human cells. These essential oils were able to inhibit the breakdown of phospholipids induced by venoms of snakes of the Bothrops genus. Both oils presented hemolytic activity, although the Mentha viridis (L). L. oil induced hemolysis only at the highest concentrations (14.6 and 29 μL·mL-1). The essential oil from M. viridis induced 3.9;8.6 and 16.2 times greater damage to human leukocyte DNA than that observed with the positive control (100 μg·μL-1 doxorubicin) at concentrations of 0.25;0.5 and 1.0 μL·mL-1, respectively. A similar effect was observed for the oil from M. pulegium (2.1, 2.5 and 15.8 times greater damage). The results extend the characterization of these essential oils and demonstrate their potential use in industries.展开更多
基金Supported by Guangxi Key Laboratory of Zhuang and Yao Medicine(GuiKeJiZi 201432)Zhuang and Yao Medicine Collaborative Innovation Center(GuiJiaoKeYan 201320)+3 种基金Guangxi Young Talent Project of Guangxi University of Chinese Medicine(2022C030)Ethnic Medicine Resources and Application Engineering Research Center in Guangxi Zhuang Autonomous Region(GuiFaGaiGaoJiHan 20202605)Key Discipline Project of Traditional Chinese Medicine in Guangxi—Zhuang Medicine(GZXK-Z-20-64)Guangxi First-class Discipline of Traditional Chinese Medicine—Ethnopharmacology(GuiJiaoKeYan 201812).
文摘[Objectives]To compare the leaf morphology and leaf venation patterns of Hedyotis auricularia L.and its adulterants,such as Mitracarpus hirtus(L.)DC.,Spermacoce pusilla Wall.and Spermacoce alata Aubl.,produced in Guangxi,so as to provide a simple and rapid identification method for the identification of H.auricularia L.in Guangxi.[Methods]LMVP(leaf morphology-venation pattern)identification method was used to study the characteristics of leaf morphology-venation pattern.[Results]Characteristics of leaf morphology-venation pattern:(i)H.auricularia L.:circular knotted-curved pinnate leaf venation without reaching the margin;the primary veins run straight without branches,the number of secondary veins is 5 to 9,and the angles included in tertiary veins are mostly near right angles or obtuse angles.(ii)M.hirtus(L.)DC.:curved pinnate leaf venation without reaching the margin;the primary veins run straight without obvious changes,the number of secondary veins is 3 to 5,and the tertiary veins are slender,and the included angles are mostly acute.(iii)S.pusilla Wall.:curved leaf venation without reaching the leaf margin;there are 4 to 5 pairs of secondary veins,most of which are opposite;the angle between the secondary veins and the primary veins in the middle and near the petiole is mostly medium acute angle,and the extension of the tertiary veins has no fixed direction.(iv)S.alata Aubl.:circular knotted-curved pinnate leaf venation;the primary veins are convex and straight without branches,and there are 4 to 6 pairs of secondary veins,alternating.The angle between secondary veins and primary veins is mostly medium acute angle,the angle between tertiary veins and secondary veins is near right angle,and the tertiary veins are mostly transversely extended.[Conclusions]The leaf morphology-venation patterns of the above-mentioned H.auricularia L.and M.hirtus(L.)DC.,S.pusilla Wall.,and S.alata Aubl.can be used as their identification features,and the identification method has certain operability,which provides an identification idea for the identification of Chinese herbal medicines.
基金Program for Changjiang Scholar and Innovative Team in University(Grant No.985-2-063-112)
文摘We quantitatively determined four nucleosides, including cytidine, uridine, guanosine, and adenosine, in Carthamus tinctorius L. and Safflower injection. Separation was performed on a Zorbax Eclipse XDB-18 column using a gradient elution with mobile phases of 0.05% trifluoroacetic acid （TFA） aqueous solution （A） and methanol （B）. The assay was carried out at a flow rate of 1 mL/min at 25 ℃ with detection at 260 nm. Cytidine, uridine, adenosine and guanosine showed good linearity in the ranges of4.02-503μg/mL （r2= 0.9998）, 9.38-1407 μg/mL （rz = 0.9999）, 80.6-8060μg/mL （r2 = 0.9999） and 2.10---630μg/mL （r2 = 0.9987） with average recoveries of 97.2%, 94.5%, 98.6% and 108.6%, respectively. The contents of cytidine, uridine, adenosine and guanosine in different Carthamus tinctorius L. and Safflower injection were significantly different. This is the first report on the quantitative determination of nucleosides in Carthamus tinctorius L. and Safflower injection.
文摘Some wild species of Oryza possess agronomi-cally useful traits such as disease/insect resis-tance and salt tolerance.However,the use ofwild Oryza species for rice improvement hasbeen hampered by the sexual incompatibility orsterility of hybrids obtained by conventionalmethods.We have used protoplast fusion as amethod to incorporate useful trait of wild O-ryza species into cultivated rice and~ obtainedsomatic hybrid plants between O.sativa and
文摘Male sterile NEA plants were identified in progenies of the radiated 92P x Aggregate-silique in Brassica napus L. in 1993. Their progeny plants from test crossing and open pollination were 100% male sterile. The double-low male sterile lines JL-4 and JL-18 were bred through successive backcrossing of the double -low variety No.4 and No. 18 in Brassica napus L.to NEA. Restorer lines 6720 and 6722 with significant heterosis in F1 were developed. The results from investigating the restoring-ntaintaining relationship and inheritance of the restorer gene indicated that JL-4 and JL-18 were different from both PolCMS and Shan 2A type, and their restorer gene was controlled by a pair of dominant genie genes.
基金Supported by the Chinese Academy of Sciences Action Plan for the Development of Western China (No. KZCX2-XB2-13)the Chinese Academy of Sciences Knowledge Innovation Program (No. KSCX2-YW-N-003)the 100 Talents Program of Chinese Academy of Sciences
文摘In order to utilize the wasted saline-sodic soils under shallow groundwater condition, a 3-year field study was carried in a field cropped with Lycium barbarum L. and irrigated by drip irrigation with saline groundwater under the water table depth of 30-40 cm in the northern Yinchuan Plain, China. Effects of cropping duration （one, two, and three years） on soil salinity, soil solution composition, and pH in three adjacent plots were investigated in 2008. Results showed that a high irrigation frequency maintained high soil water potential and subsequently facilitated infiltration and downward movement of water and salt in the crop root zone. Salt accumulated on the edges of the ridges, and soil saturated-paste electrical conductivity （ECe） was higher in the edge. Concentrations of Na^＋, Ca^2＋, Mg^2＋, Cl^-, and SO24^- in the soil increased with the soil depth as did the ECe, while HCO3 and pH had a relative uniform distribution in soil profile. As planting year increased, the ECe and soil salts in the field had a decreasing tendency, while in the root zone they decreased immediately after irrigation and then remained relatively stable in the following growing seasons. HCO3 and pH had little change with the planting year. Results suggested that the application of drip irrigation with saline water could ameliorate saline-sodic soil and provide a relatively feasible soil environment for saline-sodic soils with shallow groundwater.
文摘A new isochroman-4-one, 7,8-dihydroxy-3-methylisochroman-4-one was isolated from water soluble fraction of Musa sapientum L. Its structure was determined by spectroscopic evidences and its total synthesis has also been reported. The compound showed potent antihypertensive activity.
基金Scientific and technological innovation project of China Academy of Chinese Medical Sciences(CI2021A04409,CI2021A04404,CI2021A04405)the fundamental research funds for the central public welfare research institutes(No.ZZ13-YQ-061,ZXKT22012,ZXKT22039).
文摘An undescribed pyrrole acid,1-(4′-methoxy-4′-oxobutyl)-1 H-pyrrole-2,5-dicarboxylic acid(1)and one known pyr-role acid(2)were isolated from the fruits of Phyllanthus emblica.The structures of these compounds were elucidated via the comprehensive analyses of IR,HRESIMS,1D and 2D spectroscopic data.A series of biological assays revealed that compounds 1 and 2 could inhibit LPS-induced over-production of nitric oxide(NO),interleukin-6(IL-6),monocyte chemotactic protein 1(MCP-1)and tumor necrosis factor-α(TNF-α)by reducing the phosphorylation of extracellular regulated protein kinases(ERK)and c-Jun N-terminal kinases(JNK)in RAW 264.7 cells.Additionally,compounds 1 and 2 were found to reduce lipid deposition and increase the mRNA expression of ATP-binding cassette transporter A1 in oxidized low-density lipoprotein-treated RAW264.7 macrophages.
基金the Programa de Cooperación Territorial INTERREG V-A MAC 2014-2020 and Inv2Mac Project(MAC/4.6d/229)the partial support of FCT-Funda??o para a Ciência e a Tecnologia(Base Fund UIDB/00674/2020)+1 种基金ARDITI-Agência Regional para o Desenvolvimento da Investiga??o Tecnologia e Inova??o supported the study through the project M1420-01-0145FEDER-000005-CQM+(Madeira 14-20 Program)the Postdoc Grant(M1420-09-5369-FSE-000001,002458/2015/132)for Carla S.ALVES is also acknowledged。
文摘In this study,the fibers of invasive species Agave americana L.and Ricinus communis L.were successfully used for the first time as new sources to produce cytocompatible and highly crystalline cellulose nanofibers.Cellulose nanofibers were obtained by two methods,based on either alkaline or acid hydrolysis.The morphology,chemical composition,and crystallinity of the obtained materials were characterized by scanning electron microscopy(SEM)together with energy-dispersive X-ray spectroscopy(EDX),dynamic light scattering(DLS),X-ray diffraction(XRD),and Fourier transform infrared(FTIR)spectroscopy.The crystallinity indexes(CIs)of the cellulose nanofibers extracted from A.americana and R.communis were very high(94.1%and 92.7%,respectively).Biological studies evaluating the cytotoxic effects of the prepared cellulose nanofibers on human embryonic kidney 293 T(HEK293 T)cells were also performed.The nanofibers obtained using the two different extraction methods were all shown to be cytocompatible in the concentration range assayed(i.e.,0-500μg/mL).Our results showed that the nanocellulose extracted from A.americana and R.communis fibers has high potential as a new renewable green source of highly crystalline cellulose-based cytocompatible nanomaterials for biomedical applications.
基金This work was supported by the National Natural Science Foundation of China(Nos.31870488,41571300)the Open Fund of Cultivation State Key Laboratory of Qinba Biological Resources and Ecological Environment of Shaanxi University of Technology(No.SLGPT2019KF04-02)the project of Foreign Experts Bureau of Shaanxi province of China(No.G20200241015).
文摘The effects of different electrode on Solanum nigrum L.seed germination were determined.The result showed that germination percentage(GP)of seeds in treatment T2(titanium elec-trode)was 26.6%higher than in control(CK,without electric field).High potassium and calcium concentrations were beneficial for seed enzymatic activity in treatment T2,which could partly explain the increase in GP.Cd accumulation(μg/pot)in S.nigrum treated with any electric field was significantly higher(p<0.05)than in CK without electric field.Specif-ically,Cd accumulation under the treatment T3(stainless steel electrode)was the highest both in roots and shoots;this accumulation in shoots and roots were 74.7%and 67.4%higher for stainless steel than in CK.This increase must have been associated with a higher Cd concentration in plants and did not exert a significant effect on the biomass.In par-ticular,Cd concentrations in roots and shoots under stainless steel treatment were both significantly higher than in CK(p<0.05),which had to be related to the higher available Cd concentration in the soil in the middle region.Furthermore,it could be attributed to al-tered soil pH and other soil properties.Moreover,none of the biomasses were significantly affected(p<0.05)by different electrode materials compared to CK.
基金Supported by the National Natural Science Foundation of China(31760278)。
文摘The effects of ethanol extract of Bidens pilosa L.(EEB)on acute exercise fatigue and its underlying biochemical mechanism were investigated in this study.Sixty adult male ICR mice were divided into control,model,vitamin C(VC)100,EEB40,EEB80,and EEB160 groups,receiving VC(100 mg/kg)or EEB(40,80,160 mg/kg)for 28 days(intragastrically,I.G.).The mice underwent tailsuspension,elevated plus maze(EPM),rotarod,and loaded swimming tasks and biochemical indices were measured.There were no significant differences in body weight,tail suspension time,EPM open arm time/entries and serum cortisone levels among the groups.Compared with the model group,there was an increase in rotarod latency in the VC100/EEB80 groups and an increase in loaded swimming time in the EEB80/EEB160 groups.Furthermore,the haptic and muscle glycogen levels decreased in the model group,while the haptic glycogen levels increased in the all VC/EEB groups.Similarly,the serum lactate and creatinine levels increased in the model group,but decreased in lactate(except for EEB160),creatinine(except for EEB40)and lactate dehydrogenase in the EEB80 group.In the liver,malonaldehyde(MDA)and oxidized glutathione(GSSG)levels increased in the model group;however,glutathione reductase(GR)(except for EEB40),glutathione(GSH)and GSH/GSSG ratios increased,with GSSG levels decreasing in all VC/EEB groups.In the quadriceps,the GR levels increased in the model,whereas it decreased in the VC100,EEB40 and EEB80 groups.These results suggest that EEB has anti-acute fatigue effect,potentially attributed to mitigate metabolite accumulation,enhancing glycogen reserves,and fortifying the antioxidant mechanism.
文摘Solanum nigrum L.,belonging to the genus Solanum of Solanaceae family,is widely distributed in temperate and tropical regions of the world.It possesses the effects of clearing away heat and detoxification.A wide spectrum of chemical constituents have been isolated from the Solanum nigrum L.,including lignans,steroids,etc.The structures of the eight compounds were determined by comprehensive spectroscopic analyses.It is noteworthy that compounds 1–4,6 and 8 have been isolated from Solanum nigrum L.for the first time.All compounds were evaluated for their antioxidant activities by ABTS and DPPH assays.Based on the results,Solanum nigrum L.could be used as a new natural antioxidant in biomedical applications.
文摘Normal-phase and reversed-phase silica gel column chromatography and semi-preparative high performance liquid chromatography were applied to study the chemical constituents of the stems and twigs of the Hainan mangrove plant,Excoecaria agallocha L.A new 3,4-seco-ent-kaurane diterpenoid,named agallochanin acid A(1),and a rare ent-tetranorlabdane diterpenoid,named agallochanin acid B(2),along with four known diterpenoids were isolated.Their structures including absolute configurations were characterized via HR-ESI-MS,extensive 1D and 2D NMR investigations and calculated and experimental electronic circular dichroism(ECD)data.All isolates were tested for their inhibitory effects on NO production in lipopolysaccharide(LPS)-activated BV-2 cells.ent-3,4-Seco-17-oxo-kaur-4(19),15(16)-dien-3-oic acid(3)and ent-15-hydroxy-labda-8(17),13E-dien-3-one(5)exhibited considerable anti-neuroinflammatory activities with IC50 values of(14.32±1.99)and(4.24±0.37)μmol/L.
基金Supported by Program of Key Laboratory of Zhuang and Yao Medicine[Gui Ke Ji Zi[2014]No.32]Project of Guangxi University of Chinese Medicine(H2014015).
文摘[Objectives]To make pharmacognostic identification of Euphorbia maculata L.and its related species.[Methods]The classical pharmacognostic identification method was adopted.[Results]The four main medicinal materials are very similar,the fluff can be seen in E.maculata and Euphorbia thymifolia L.,but not in Euphorbia prostrata Ait.and Euphorbia taihsiensis(Chaw et Koutnik)Oudejians;the tissue structure is basically the same;except for E.taihsiensis without non-glandular hairs,the powder has secretory cells,laticifers,cells,calcium oxalate crystals,fibers,vessels,and seed coat fragments.Through ultrasonic extraction with 80%ethanol,extraction with isobutanol,extending with chloroform∶ethyl acetate∶formic acid(5∶3∶0.3),developing color with 3%aluminum trichloride ethanol solution,under ultraviolet light(365 nm),the fluorescent spots of the same color appeared on the corresponding position of the chromatogram of the reference substance(quercetin,kaempferol).[Conclusions]It is not easy to distinguish the four medicinal materials by character identification and microscopic identification,while the thin-layer chromatography(TLC)is more reliable.
文摘Botanical morphological characteristics of Physalis alkekengi L.is first introduced,and its medicinal and edible value is elaborated.Cultivation techniques of P.alkekengi L.are mainly introduced.The research aims to provide a certain basis for the better development and application of P.alkekengi L.
基金National Natural Science Foundation of China(81560702)Joint Research Project of Inner Mongolia Minzu University and Mongolian Academy of Sciences(NMDGJ0014)。
文摘On the basis of research on the origin of Mongolian medicine Veratrum nigrum L.,this paper summarizes the research situation of Mongolian medicine Veratrum nigrum L.from the aspects of clinical application,chemical composition,pharmacological action and toxicological action,so as to provide a reference basis for further research and further development and utilization of Mongolian medicine.
文摘This paper introduces the biological characteristics,medicinal value,chemical component,and pharmacological effects of Crocus sativus L.,and explores its therapeutic effects on cardiovascular and cerebrovascular diseases such as angina pectoris and coronary heart disease.
基金the College Student Innovation and Entrepreneurship Training Program(X202010163166).
文摘Sophora alopecuroides L.,a perennial plant belonging to Leguminosae family,is used as medicinal materials to treat a variety of skin diseases.Its chemical components are complex and diverse,mainly including alkaloids and flavonoids.Modern pharmacological studies have found that the isolated compounds from S.alopecuroides have a variety of pharmacological activities.In particular,alkaloids represented by matrine,aloperine and oxymatrine have significant anti-inflammatory,anti-viral,anti-tumor,anti-bacterial and immunomodulatory activities.This paper summarized the chemical constituents and pharmacological activities of S.alopecuroides,aiming to provide the basis for further research.
文摘Parkinson’s disease(PD)is a common neurodegenerative disease.Despite a great deal of research on this disease,there is still no definitive treatment plan.Literature investigations have shown that Piper nigrum L.has a protective effect on the nerve center.Therefore,network pharmacology was used in this study to investigate the therapeutic potential of P.nigrum for PD.As a result,six active alkaloid ingredients and four potential targets were obtained.This study laid the foundation for finding the lead compounds from P.nigrum to treat PD.
基金the support of the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico(CNPq) the Fundacao de Amparo a Pesquisa do Estado de Minas Gerais(FAPEMIG)for financial support the Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior(CAPES)for a PVNS.
文摘The essential oils from Mentha viridis (L). L. and Mentha pulegium L. were studied to assess their inhibitory potential on phospholipase from snake venoms and to determine their cytogenotoxic action on human cells. These essential oils were able to inhibit the breakdown of phospholipids induced by venoms of snakes of the Bothrops genus. Both oils presented hemolytic activity, although the Mentha viridis (L). L. oil induced hemolysis only at the highest concentrations (14.6 and 29 μL·mL-1). The essential oil from M. viridis induced 3.9;8.6 and 16.2 times greater damage to human leukocyte DNA than that observed with the positive control (100 μg·μL-1 doxorubicin) at concentrations of 0.25;0.5 and 1.0 μL·mL-1, respectively. A similar effect was observed for the oil from M. pulegium (2.1, 2.5 and 15.8 times greater damage). The results extend the characterization of these essential oils and demonstrate their potential use in industries.
