Objectives:Among cutaneous malignancies,melanoma stands out for its particularly aggressive nature,with therapeutic interventions becoming notably limited once the disease progresses.In this research,we investigate th...Objectives:Among cutaneous malignancies,melanoma stands out for its particularly aggressive nature,with therapeutic interventions becoming notably limited once the disease progresses.In this research,we investigate the tumor-suppressing capabilities of water-extracted Korean mistletoe(Viscum album var.coloratum)and its purified lectin component(V.album var.coloratum agglutinin,VCA)using two distinct mouse melanoma models:B16BL6 and B16F10 cell lines.Methods:The impact of water extract and VCA treatments on melanoma cells was assessed through multiple experimental approaches,examining cellular survival rates,programmed cell death pathways,multicaspase activity,and cell cycle distribution patterns.To elucidate the interconnections among various cellular responses,we employed a suite of statistical techniques encompassing correlation studies,principal component analysis(PCA)-based dimensionality reduction,and dendrogram-based clusteringmethodologies.Results:Thewater extract exhibited dosedependent cytotoxicity with IC50 values of 372.3±8.7μg/mL and 202.5±8.4μg/mL for B16BL6 and B16F10 cells,respectively.VCA showed more significant effects,with IC50 values of 0.1992±0.0041 and 0.1981±0.0098μg/mL for B16BL6 and B16F10 cells,respectively.Both agents induced substantial apoptosis with a significant progression from early to late apoptotic stages,reaching up to 59.4%total apoptotic cells for VCA treatment.This was confirmed by strong multicaspase activation,particularly in VCA-treated cells(up to 88.4%caspase-positive cells).The water extract showed modest effects on cell cycle distribution,with increases in G0/G1 phase(74.6%)in B16BL6 cells and S phase(19.2%)in B16F10 cells,while VCA treatment resulted in G2/M phase reduction(10.0%)in B16F10 cells.Correlation analysis revealed strong negative associations between cell viability and caspase activity(r=−0.843 to−0.878),while hierarchical clustering demonstrated distinct response patterns between low and high concentrations of both agents.Effect size analysis confirmed strong treatment impacts on cell viability(d=−5.89 to−6.12)and caspase activation(d=3.45 to 5.23).Conclusion:These findings suggest that Korean mistletoe water extract and its isolated lectin may affect both primary and metastatic melanoma cells through distinct mechanisms,demonstrating particular potency in caspase-dependent apoptosis induction.Our findings establish a robust foundation for developing novel therapeutic interventions derived from natural compounds to combat malignant melanoma with high metastatic potential.展开更多
本文研究两组同龄新疆杨(Populus alba L.var.pyramidalis)(一组土壤含盐量较低,树木长势良好,另一组土壤盐分高,树木长势差)对盐分离子的吸收选择性系数和从根至边材,从边材至叶的运输选择性系数,结合盐分离子在树体各部位的分布状况,...本文研究两组同龄新疆杨(Populus alba L.var.pyramidalis)(一组土壤含盐量较低,树木长势良好,另一组土壤盐分高,树木长势差)对盐分离子的吸收选择性系数和从根至边材,从边材至叶的运输选择性系数,结合盐分离子在树体各部位的分布状况,探讨新疆杨对长期盐胁迫的生理反应。主要结论为:盐胁迫下新疆杨通过选择性吸收K+,抑制过多Na+进入根系,新疆杨对盐分离子的运输选择性主要体现在根向边材的运输中,新疆杨根系对Na+具有较强的截留作用,对Cl-和SO42-也有一定截留作用。展开更多
文摘Objectives:Among cutaneous malignancies,melanoma stands out for its particularly aggressive nature,with therapeutic interventions becoming notably limited once the disease progresses.In this research,we investigate the tumor-suppressing capabilities of water-extracted Korean mistletoe(Viscum album var.coloratum)and its purified lectin component(V.album var.coloratum agglutinin,VCA)using two distinct mouse melanoma models:B16BL6 and B16F10 cell lines.Methods:The impact of water extract and VCA treatments on melanoma cells was assessed through multiple experimental approaches,examining cellular survival rates,programmed cell death pathways,multicaspase activity,and cell cycle distribution patterns.To elucidate the interconnections among various cellular responses,we employed a suite of statistical techniques encompassing correlation studies,principal component analysis(PCA)-based dimensionality reduction,and dendrogram-based clusteringmethodologies.Results:Thewater extract exhibited dosedependent cytotoxicity with IC50 values of 372.3±8.7μg/mL and 202.5±8.4μg/mL for B16BL6 and B16F10 cells,respectively.VCA showed more significant effects,with IC50 values of 0.1992±0.0041 and 0.1981±0.0098μg/mL for B16BL6 and B16F10 cells,respectively.Both agents induced substantial apoptosis with a significant progression from early to late apoptotic stages,reaching up to 59.4%total apoptotic cells for VCA treatment.This was confirmed by strong multicaspase activation,particularly in VCA-treated cells(up to 88.4%caspase-positive cells).The water extract showed modest effects on cell cycle distribution,with increases in G0/G1 phase(74.6%)in B16BL6 cells and S phase(19.2%)in B16F10 cells,while VCA treatment resulted in G2/M phase reduction(10.0%)in B16F10 cells.Correlation analysis revealed strong negative associations between cell viability and caspase activity(r=−0.843 to−0.878),while hierarchical clustering demonstrated distinct response patterns between low and high concentrations of both agents.Effect size analysis confirmed strong treatment impacts on cell viability(d=−5.89 to−6.12)and caspase activation(d=3.45 to 5.23).Conclusion:These findings suggest that Korean mistletoe water extract and its isolated lectin may affect both primary and metastatic melanoma cells through distinct mechanisms,demonstrating particular potency in caspase-dependent apoptosis induction.Our findings establish a robust foundation for developing novel therapeutic interventions derived from natural compounds to combat malignant melanoma with high metastatic potential.
文摘本文研究两组同龄新疆杨(Populus alba L.var.pyramidalis)(一组土壤含盐量较低,树木长势良好,另一组土壤盐分高,树木长势差)对盐分离子的吸收选择性系数和从根至边材,从边材至叶的运输选择性系数,结合盐分离子在树体各部位的分布状况,探讨新疆杨对长期盐胁迫的生理反应。主要结论为:盐胁迫下新疆杨通过选择性吸收K+,抑制过多Na+进入根系,新疆杨对盐分离子的运输选择性主要体现在根向边材的运输中,新疆杨根系对Na+具有较强的截留作用,对Cl-和SO42-也有一定截留作用。
