To explore the material basis and mechanisms of the anti-inflammatory effects of Hibiscus mutabilis L..The active ingredients and potential targets of Hibiscus mutabilis L.were obtained through the literature review a...To explore the material basis and mechanisms of the anti-inflammatory effects of Hibiscus mutabilis L..The active ingredients and potential targets of Hibiscus mutabilis L.were obtained through the literature review and SwissADME platform.Genes related to the inflammation were collected using Genecards and OMIM databases,and the intersection genes were submitted on STRING and DAVID websites.Then,the protein interaction network(PPI),gene ontology(GO)and pathway(KEGG)were analyzed.Cytoscape 3.7.2 software was used to construct the“Hibiscus mutabilis L.-active ingredient-target-inflammation”network diagram,and AutoDockTools-1.5.6 software was used for the molecular docking verification.The antiinflammatory effect of Hibiscus mutabilis L.active ingredient was verified by the RAW264.7 inflammatory cell model.The results showed that 11 active components and 94 potential targets,1029 inflammatory targets and 24 intersection targets were obtained from Hibiscus mutabilis L..The key anti-inflammatory active ingredients of Hibiscus mutabilis L.are quercetin,apigenin and luteolin.Its action pathway is mainly related to NF-κB,cancer pathway and TNF signaling pathway.Cell experiments showed that total flavonoids of Hibiscus mutabilis L.could effectively inhibit the expression of tumor necrosis factor(TNF-α),interleukin 8(IL-8)and epidermal growth factor receptor(EGFR)in LPS-induced RAW 264.7 inflammatory cells.It also downregulates the phosphorylation of human nuclear factor ĸB inhibitory protein α(IĸBα)and NF-κB p65 subunit protein(p65).Overall,the anti-inflammatory effect of Hibiscus mutabilis L.is related to many active components,many signal pathways and targets,which provides a theoretical basis for its further development and application.展开更多
Radish(Raphanus sativus L.)is an important cruciferous root vegetable,with bolting regulated by multiple genes.However,the genetic mechanisms underlying bolting regulation remain unclear.Here,the genome of the cultiva...Radish(Raphanus sativus L.)is an important cruciferous root vegetable,with bolting regulated by multiple genes.However,the genetic mechanisms underlying bolting regulation remain unclear.Here,the genome of the cultivar C60213 is assembled into a high-quality,gap-free telomere-to-telomere structure,spanning nine chromosomes and totaling 472.71 Mb,using a combination of Oxford Nanopore,PacBio,and Hi-C sequencing technologies.It identifies 49,768 protein-coding genes,97.38%of which are functionally annotated.Repetitive sequences constitute 59.72%of the genome,primarily comprising long terminal repeats.A high-density genetic linkage map is constructed using an F2 population derived from a cross between early-and late-bolting radishes,identifying seven major quantitative trait loci associated with bolting and flowering.RNA-seq and quantitative real-time PCR analysis reveal that the RsMIPS3 gene is found to be associated with bolting,with its expression decreasing during this process.Notably,RsMIPS3 overexpression in Arabidopsis delays bolting,confirming its role in regulating bolting time.These findings advance radish genome research and provide a valuable target for breeding late-bolting varieties.展开更多
Industrial activities have caused widespread arsenic(As)contamination in soil and medicinal crops across south-ern Asia.This study constructed interplanting systems combing medicinal crops with Pteris vittata L.,aimin...Industrial activities have caused widespread arsenic(As)contamination in soil and medicinal crops across south-ern Asia.This study constructed interplanting systems combing medicinal crops with Pteris vittata L.,aiming to mitigate the risk of As exposure in medicinal crops,while simultaneously achieving ecological remediation of contaminated soil.The results revealed that interplanting with P.vittata significantly enhanced the yield of Gynos-temma pentaphyllum by 31.90%(P<0.05)compared with monoculture systems.Under monoculture conditions,the As concentration in G.pentaphyllum leaves reached 2.34 mg/kg,exceeding the national food safety standard(GB2762–2017,2 mg/kg).However,interplanting with P.vittata effectively reduced the As concentration in G.pentaphyllum leaves to 1.82 mg/kg.Furthermore,the interplanting of P.vittata with Rhus chinensis significantly inhibited As translocation from belowground to aboveground tissues in R.chinensis.Compared to monoculture,the stem biomass of P.vittata was significantly increased by 57.50%and 70.32%when interplanted with G.pentaphyllum and Cassia obtusifolia L.(P<0.05).So the As enrichment of P.vittata was enhanced in interplanting systems,which is beneficial for the As removal from contaminated soil.The study demonstrated that interplant-ing primarily regulates plant As uptake through modifications of rhizosphere physicochemical properties and As bioavailability,especially for water-soluble As that is easily absorbed by plants.In conclusion,the interplant-ing models integrating medicinal crops and P.vittata can achieve the goal of“remediating while producing”in As-contaminated soil.展开更多
The cultivation of Soledad pepper(Capsicum annuum L.)is essential in Oaxaca and Veracruz,but it faces issues with pests and diseases,which affect yield and cause economic losses.To mitigate these impacts,farmers have ...The cultivation of Soledad pepper(Capsicum annuum L.)is essential in Oaxaca and Veracruz,but it faces issues with pests and diseases,which affect yield and cause economic losses.To mitigate these impacts,farmers have started using biostimulants such as chitosan and plant growth promoting bacteria instead of agrochemicals due to their environmental and health benefits.This study evaluated the effect of Bacillus subtilis and chitosan,both individually and combined,on the growth,yield,and fruit quality of Soledad pepper under greenhouse conditions.Four treatments were applied at different stages of the crop cycle:Q(Chitosan),BS(Bacillus subtilis),Q+BS(Chitosan+Bacillus subtilis),and T(Control).The results showed that the combination of chitosan and Bacillus subtilis significantly improved plant growth,especially in height and stem diameter.The chitosan treatment produced the highest number of fruits and plant weight,while the combination of chitosan and Bacillus subtilis enhanced fruit quality,increasing characteristics such as size,weight,pericarp thickness,and physicochemical parameters,notably in brix degrees,citric acid percentage,and pH,outperforming the individual treatments and the control.展开更多
Background:Epidemiological studies have confirmed that longer exposure to insecticides like cypermethrin(CYP)significantly increases the risk of male reproductive toxicity.Crocus sativus L.has been recognized due to i...Background:Epidemiological studies have confirmed that longer exposure to insecticides like cypermethrin(CYP)significantly increases the risk of male reproductive toxicity.Crocus sativus L.has been recognized due to its therapeutic properties,but its exact role and molecular mechanisms in treatment of reproductive dysfunction remain unclear.Methods:During this study,36 rats were randomly divided into six groups(n=6):control,CYP-induced(60 mg/kg),standard(leuprolide 3 mg/kg)and three treatment groups receiving aqueous,ethanolic,and oil extracts(50 mg/kg or 20 mL/kg)for post-toxicity induction.Results:The finding represented that exposure of CYP significantly increased oxidative stress,disrupted testicular architecture,and markedly reduced testosterone levels(P<0.05).Importantly,Crocus sativus L.treatment alleviated these changes by increasing the expression of Nrf2(nuclear factor erythroid 2-related factor 2),restoring the activity of antioxidant enzymes,and enhancing testicular histomorphology.Surprisingly,molecular docking established a high binding affinity of Crocus sativus L.phytoconstituents such as gallic acid,cinnamic acid and quercetin to the Nrf2-Keap1 complex.It is worth noting that,Crocus sativus L.exhibited a high level of protection against reproductive toxicity caused by CYP in male rats,which was mediated by the activation of Nrf2 pathway,reduction of oxidative damage,and favorable ADMET characteristics.Conclusion:Notably,this research provides a more valid,safe,and effective method of developing new drugs for reproductive disorders,however,further investigation is needed to support the research findings and implement it in clinical practice.展开更多
Dry fig is a traditional healthy snack and has important economic value in a number of Mediterranean and Middle Eastern countries.Cultivars with no anthocyanin accumulation in the fruit peel are preferred for dry fig ...Dry fig is a traditional healthy snack and has important economic value in a number of Mediterranean and Middle Eastern countries.Cultivars with no anthocyanin accumulation in the fruit peel are preferred for dry fig production.R2R3-MYB transcription factors have promotive or repressive regulatory roles in plant anthocyanin biosynthesis.In this study,113 R2R3-MYB genes were identified in Ficus carica,3 of which were assigned to the S4 subfamily of flavonoid-biosynthesis repressors.FcMYB57 was further recruited as a candidate anthocyaninbiosynthesis repressor based on its sequence features and expression,which was significantly negatively correlated with that of anthocyanin-biosynthesis structural genes.Transient overexpression of FcMYB57 in strawberry totally inhibited fruit pigmentation and significantly increased fruit firmness.The metabolomic analysis confirmed a significant reduction in the contents of cyanidin-3-O-glucoside and pelargonidin-3-O-glucoside,as well as other flavonoids,and transmission electron microscopy revealed an increment in cell-wall thickness.Transcriptome analysis showed downregulation of anthocyanin-biosynthesis structural genes and upregulation of genes related to xylan synthesis.Yeast one-hybrid and dual luciferase assays demonstrated a negative regulatory effect of FcMYB57 on the promoter of FcUFGT3(UDP glucose-flavonoid 3-O-glcosyl-transferase).Yeast two-hybrid assay showed that FcMYB57 does not interact with FcbHLH42,3,14,MYC2,or FcTTG1,all of which have a previously identified or predicted role in flavonoid biosynthesis,however,interaction was detected with FcTPL(Topless),suggesting that FcMYB57 serves as an active repressor of anthocyanin biosynthesis.This is the first identification of an anthocyaninbiosynthesis repressor in fig,with a possible role in fig fruit quality.展开更多
Long-chain acyl-CoA synthetase(LACS),belonging to the AMP-bound superfamily,catalyzes the activation of long-chain fatty acids to their coenzyme A esters.Candidate Gene Association analysis showed that LACS8 was not o...Long-chain acyl-CoA synthetase(LACS),belonging to the AMP-bound superfamily,catalyzes the activation of long-chain fatty acids to their coenzyme A esters.Candidate Gene Association analysis showed that LACS8 was not only related to the content of fatty acid,but also,surprisingly,to the content of glucosinolates.Here,we cloned BnaLACS8A03 from rapeseed,and demonstrated its association with the glucosinolates content.We found that BnaLACS8A03 was widely expressed in all tissues and organs,and significantly highly expressed in leaf and flower,which are the sites of glucosinolate synthesis.Bioinformatics predicted that it was located in peroxisome,where theβ-oxidation of fatty acids mainly occurred,indicating that BnaLACS8A03 was involved in the metabolism of fatty acids.Phenotypic studies of RNA interference(RNAi)lines revealed that down-regulation of BnaLACS8A03 in rapeseed significantly reduced the glucosinolates content.In addition,the expression of glucosinolates biosynthesis-related genes and transport-related genes is altered in BnaLACS8A03-RNAi plants,suggesting that a possible crosstalk regulation mechanism exists in the regulation network of glucosinolate mechanism.Our results demonstrate that glucosinolates content can be manipulated by altering the expression of long chain acyl-Coenzyme A synthase gene BnaLACS8A03,which provide an available genetic resource for low glucosinolate breeding practice in rapeseed.展开更多
Ceratopteris thalictroides (L.) Brongn (C. thalictroides), a fern from the Pteridaceae family, isan edible species that remains largely underutilized, even though it possesses a diverserange of applications. This revi...Ceratopteris thalictroides (L.) Brongn (C. thalictroides), a fern from the Pteridaceae family, isan edible species that remains largely underutilized, even though it possesses a diverserange of applications. This review aims to provide a comprehensive overview of itsgeographical distribution, ethnomedicinal applications, phytochemical constituents,pharmacological activities, and ecological importance. Various phytochemical analysesidentified the presence of phenols, flavonoids, alkaloids, terpenoids, and fatty acids from C.thalictroides, responsible for its multiple pharmacological properties, including antioxidant,antimicrobial, anti-inflammatory, anti-diabetic, and anticancer activities. Additionally, itsunique antheridiogen activity makes it a valuable model organism for plant genetics andreproductive studies. This review systematically summarizes the phytopharmacologicalpotential and ecological significance of C. thalictroides. The plant holds promise as a sourceof novel therapeutics and as a sustainable bioresource for pharmaceutical andenvironmental applications.展开更多
Lablab purpureus(L.)Sweet(L.purpureus)has been reported to alleviate diarrhea,although the precise mechanism remains unclear.This study identified the primary active components of L.purpureus utilizing ultraperformanc...Lablab purpureus(L.)Sweet(L.purpureus)has been reported to alleviate diarrhea,although the precise mechanism remains unclear.This study identified the primary active components of L.purpureus utilizing ultraperformance liquid chromatography/tandem mass spectrometry(UPLC-MS/MS).A rhubarb-induced diarrhea model in rats was utilized to assess the therapeutic efficacy of L.purpureus.Alterations in gut microbiota and fecal metabolism were analyzed via 16S r DNA analysis and targeted metabolomics.Flora elimination and fecal transplantation techniques were employed to deepen understanding of the role of intestinal flora in L.purpureus treatment.The study findings indicated that the main constituents of L.purpureus included trigonelline,piperidinic acid,and L-(–)-malic acid,among others.L.purpureus treatment significantly alleviated all diarrhea symptoms in rats,encompassing reduced fecal water content,weight loss,shortened colon length,diminished histological damage,and decreased inflammatory factors.Furthermore,L.purpureus significantly enhanced the expression of tight junction markers and restored the dysregulated intestinal flora in diarrheic rats by increasing Prevotella and reducing Lactobacillus.Additionally,the production of propionic acid and other short-chain fatty acids(SCFAs)increased in diarrheic rats treated with L.purpureus,suggesting a substantial alteration in the intestinal environment.Crucially,the protective efficacy of L.purpureus diminishes in the absence of gut flora.Subsequent fecal transplantation tests demonstrated that feces from the L.purpureus-treated group alleviated rhubarb-induced diarrhea,emphasizing the pivotal role of gut microbiota in the antidiarrheal efficacy of L.purpureus.In conclusion,our findings elucidate the underlying mechanisms of L.purpureus'antidiarrheal action and its beneficial impact on intestinal microflora.Moreover,these results provide compelling evidence supporting the therapeutic use of L.purpureus for the treatment of diarrhea and its associated complications.展开更多
[Objectives]To compare the leaf morphology and leaf venation patterns of Hedyotis auricularia L.and its adulterants,such as Mitracarpus hirtus(L.)DC.,Spermacoce pusilla Wall.and Spermacoce alata Aubl.,produced in Guan...[Objectives]To compare the leaf morphology and leaf venation patterns of Hedyotis auricularia L.and its adulterants,such as Mitracarpus hirtus(L.)DC.,Spermacoce pusilla Wall.and Spermacoce alata Aubl.,produced in Guangxi,so as to provide a simple and rapid identification method for the identification of H.auricularia L.in Guangxi.[Methods]LMVP(leaf morphology-venation pattern)identification method was used to study the characteristics of leaf morphology-venation pattern.[Results]Characteristics of leaf morphology-venation pattern:(i)H.auricularia L.:circular knotted-curved pinnate leaf venation without reaching the margin;the primary veins run straight without branches,the number of secondary veins is 5 to 9,and the angles included in tertiary veins are mostly near right angles or obtuse angles.(ii)M.hirtus(L.)DC.:curved pinnate leaf venation without reaching the margin;the primary veins run straight without obvious changes,the number of secondary veins is 3 to 5,and the tertiary veins are slender,and the included angles are mostly acute.(iii)S.pusilla Wall.:curved leaf venation without reaching the leaf margin;there are 4 to 5 pairs of secondary veins,most of which are opposite;the angle between the secondary veins and the primary veins in the middle and near the petiole is mostly medium acute angle,and the extension of the tertiary veins has no fixed direction.(iv)S.alata Aubl.:circular knotted-curved pinnate leaf venation;the primary veins are convex and straight without branches,and there are 4 to 6 pairs of secondary veins,alternating.The angle between secondary veins and primary veins is mostly medium acute angle,the angle between tertiary veins and secondary veins is near right angle,and the tertiary veins are mostly transversely extended.[Conclusions]The leaf morphology-venation patterns of the above-mentioned H.auricularia L.and M.hirtus(L.)DC.,S.pusilla Wall.,and S.alata Aubl.can be used as their identification features,and the identification method has certain operability,which provides an identification idea for the identification of Chinese herbal medicines.展开更多
Passiflora incarnata L.,commonly known as passionflower,is traditionally cultivated as an ornamental plant but has demonstrated diverse therapeutic potential.Its pharmacological effects are attributed to bioactive com...Passiflora incarnata L.,commonly known as passionflower,is traditionally cultivated as an ornamental plant but has demonstrated diverse therapeutic potential.Its pharmacological effects are attributed to bioactive compounds such as flavonoids and alkaloids,which influence multiple biological pathways.This review aims to summarise and critically analyse recent findings on the pharmacological properties of Passiflora incarnata L.,focusing on its neuropsychiatric,antioxidant,antimicrobial,and anticancer activities.A targeted literature search was conducted in PubMed,Scopus,Web of Science,and Google Scholar for peer-reviewed publications between 2000 to 2025.Relevant articles were screened,and a more appropriate article related to the objective of the review was selected.Some classical papers are also cited as per the requirement of the topic.Passiflora incarnata L.showed multifunctional medicinal properties with various applications in neuropsychiatry,oxidative stress management,antimicrobial agent,and as an anticancer agent.The U.S.Food and Drug Administration categorizes passionflower extracts as“generally recognized as safe”.However,most evidence remains preclinical,with methodological variation limiting generalisation.Standardised formulation,robust clinical trials,and in-depth in vivo studies are essential to establish its therapeutic relevance and safety in modern medicine.展开更多
Nigella sativa L.seeds have been traditionally utilized in Chinese folk medicine for centuries to treat vitiligo.This study revealed that the ethanolic extract of Nigella sativa L.(HZC)enhances melanogenesis and mitig...Nigella sativa L.seeds have been traditionally utilized in Chinese folk medicine for centuries to treat vitiligo.This study revealed that the ethanolic extract of Nigella sativa L.(HZC)enhances melanogenesis and mitigates oxidative stress-induced cellular senescence and dysfunction in melanocytes.In accordance with established protocols,the ethanol fraction from Nigella sativa L.seeds was extracted,concentrated,and lyophilized to evaluate its herbal effects via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assays,tyrosinase activity evaluation,measurement of cellular melanin contents,scratch assays,senescence-associatedβ-galactosidase(SA-β-gal)staining,enzyme-linked immunosorbent assay(ELISA),and Western blot analysis for expression profiling of experimentally relevant proteins.The results indicated that HZC significantly enhanced tyrosinase activity and melanin content while notably increasing the protein expression levels of Tyr,Mitf,and gp100 in B16F10 cells.Furthermore,HZC effectively mitigated oxidative stress-induced cellular senescence,improved melanocyte condition,and rectified various functional impairments associated with melanocyte dysfunction.These findings suggest that HZC increases melanin synthesis in melanocytes through the activation of the MAPK,PKA,and Wnt signaling pathways.In addition,HZC attenuates oxidative damage induced by H2O2 therapy by activating the nuclear factor E2-related factor 2-antioxidant response element(Nrf2-ARE)pathway and enhancing the activity of downstream antioxidant enzymes,thus preventing premature senescence and dysfunction in melanocytes.展开更多
From the seeds of Peganum harmala L.,three new alkaloids ofβ-carboline were isolated.Among them,peganumiums A(1)and B(2)were dimers with specific new scaffolds,all with long conjugated systems.Peganumium A and peganu...From the seeds of Peganum harmala L.,three new alkaloids ofβ-carboline were isolated.Among them,peganumiums A(1)and B(2)were dimers with specific new scaffolds,all with long conjugated systems.Peganumium A and peganumium C(3)were ionic alkaloid salts and peganumium B was a hexacycliccondensed alkaloid.The biosynthetic pathways of the three compounds above were also speculated.A preliminary cytotoxicity assay revealed that peganumium B had strong in vitro antiproliferative ability against a variety of cancer cells.The analysis of^(1)H nuclear magnetic resonance(NMR)metabolomics suggested that the antiproliferative mechanism of peganumium B could be associated with the biosynthesis of phenylalanine,tyrosine and tryptophan,the metabolism of glycine,serine,and threonine,the metabolism of taurine and hypotaurine,and the metabolism of nicotinate and nicotinamide.In addition,peganumium B could reduce the mitochondrial content of body-wall muscle cells of a Caenorhabditis elegans(C.elegans)strain in vivo.展开更多
Bentonite is a very useful material for improving soil properties,which enhances the ability of plants to grow and produce in different conditions.The experiment was carried out in an agricultural nursery in one of th...Bentonite is a very useful material for improving soil properties,which enhances the ability of plants to grow and produce in different conditions.The experiment was carried out in an agricultural nursery in one of the areas of the City of Diwaniyah,in a house covered with green netting,with a shade rate of 25%,to study the effect of bentonite and humic acid on the growth and flowering of a Catharanthus roseus L.plant in sandy soil.The experiment included two factors:the first factor was bentonite clay,and the second factor was humic acid.Using a randomized complete block design(R.C.B.D)with three replications,data were analyzed using the analysis of variance(ANOVA)method,and comparison was made according to the least significant difference(L.S.D)test at a probability level of 0.05.The experiment consisted of adding bentonite clay at 0,2,6,and 8 g L-1,humic acid at 0,0.5,1,and 10 g L-1.The results showed that adding bentonite clay and humic acid to sandy soil can have a significant positive effect on the growth and flowering of the Catharanthus roseus plant grown in poor sandy soil conditions.Bentonite,clay and humic acid were added at concentrations of 8 and 10 g L-1,which led to an increase in plant height and number of leaves and leaf area.They reached 30.07,23.84 cm2,76.62,63.42 cm2 for leaf-1 and 24.73,20.22 cm2 for leaf-1,respectively.The results also showed an increase in the content of nitrogen(N),phosphorus(P),and potassium(K)in leaves by 2.27,1.92,1.99%and 1.51,1.22,1.77%.This also led to an increase in chlorophyll pigment and anthocyanin at the highest concentration and gave the highest value.Therefore,adding bentonite and humic acid together gave the highest values in vegetative and chemical characteristics,compared to treatments without addition.展开更多
One of agriculture’s major challenges is the low efficiency of phosphate(Pi)use,which leads to increased costs,harmful environmental impacts,and the depletion of phosphorus(P)resources.The TaPHT1;6 gene,which encodes...One of agriculture’s major challenges is the low efficiency of phosphate(Pi)use,which leads to increased costs,harmful environmental impacts,and the depletion of phosphorus(P)resources.The TaPHT1;6 gene,which encodes a high-affinity Pi transporter(PHT),plays a crucial role in Pi absorption and transport.In this study,the promoter and coding regions of three TaPHT1;6 gene copies on chromosomes 5A,5B,and 5D were individually amplified and sequenced from 167 common wheat(Triticum aestivum L.)cultivars.Sequence analysis revealed 16 allelic variation sites within the promoters of TaPHT1;6-5B among these cultivars,forming three distinct haplotypes:Hap1,Hap2,and Hap3.Field trials were conducted over two years to compare wheat genotypes with these haplotypes,focusing on assessing plant dry weight,grain yield,P content,Pi fertilizer absorption efficiency,and Pi fertilizer utilization efficiency.Results indicated that Hap3 represented the favored Pi-efficient haplotype.Dual-luciferase reporter assay demonstrated that the Hap3 promoter,carrying the identified allelic variation sites,exhibited higher gene-driven capability,leading to increased expression levels of the TaPHT1;6-5B gene.We developed a distributed cleaved amplified polymorphic site marker(dCAPS-571)to distinguish Hap3 from the other two haplotypes based on these allelic variation sites,presenting an opportunity for breeding Pi-efficient wheat cultivars.This study successfully identified polymorphic sites on TaPHT1;6-5B associated with Pi efficiency and developed a functional molecular marker to facilitate future breeding endeavors.展开更多
[Objective] The research aimed to lay a foundation for the screening of cell lines producing secondary metabolites of Brucea javanica(L.)Merr.[Method] The insecticidal activities of the extracts from branch and 3 diff...[Objective] The research aimed to lay a foundation for the screening of cell lines producing secondary metabolites of Brucea javanica(L.)Merr.[Method] The insecticidal activities of the extracts from branch and 3 different types of calluses of Brucea javanica(L.)Merr.was detected through methods of leaf disc and potted seedlings against the diamond back moth.[Result] Extracts from four kinds of Brucea javanica(L.)Merr.tissues assumed both the activities of antifeedant and oviposition deterrency against the diamond back moth.Antifeedant effect of extracts was in turn the callus C< callus B< callus A< branches.Oviposition deterrency activity of the extracts was in turn the callus A> branch > callus B>callus C.The insecticidal activities of callus A and B were higher than that of the callus C.[Conclusion] The results show that insecticidal activity of callus and its growth rate is inversely proportional.展开更多
The aim of the present study was to evaluate the effects of baobab seed washing and origin on the chemical composition of the oil extracted by pressing. Six (6) oil samples were obtained from seeds of three (3) geogra...The aim of the present study was to evaluate the effects of baobab seed washing and origin on the chemical composition of the oil extracted by pressing. Six (6) oil samples were obtained from seeds of three (3) geographical origins. The identification and quantification of fatty acids and the polyphenolic profile were carried out by GC-MS and HPLC-UV, respectively. Analysis of fatty acid methyl esters allowed the identification and quantification of 18 fatty acids. Oils from unwashed seeds were richer in palmitic (C16:0), stearic (C18:0), oleic (C18:1) and arachidic (C20:0) acids. In addition, HPLC-UV analysis at 279 nm shows that oils from unwashed seeds are richer in tyrosol, hydroxytyrosol and caffeic acid. With regard to the polyphenolic profile, gallic acid and quercetin were not detected in these baobab oils. Principal component analysis of fatty acid and phenolic compound content showed that oils from unwashed seeds would best preserve their chemical and associated potential bioactive characteristics.展开更多
Guava(Psidium guajava L.),a plant belonging to the Myrtaceae family,holds significant medicinal and nutritional values.Leaves of the plant are described to be elliptical with a dark green color and are utilized in the...Guava(Psidium guajava L.),a plant belonging to the Myrtaceae family,holds significant medicinal and nutritional values.Leaves of the plant are described to be elliptical with a dark green color and are utilized in the treatment of gastrointestinal and respiratory issues along with increasing the platelet count in patients suffering from dengue.In this review paper,the nutritional composition and bioactive compounds of guava leaves,including proteins,vitamins,minerals,and polysaccharides,have been reviewed.The methods of extraction of bioactive compounds from guava leaves and their bioactivities,including antioxidant,anti-diabetic,and anti-cancer potential,have been explored.Further,encapsulation techniques for improving the transport of bioactive compounds and living cells into foods have been studied.The application of guava leaves in different food systems such as herbal tea,pork sausage,chocolates,jelly,meals for chickens,functional beverages,and as antibacterial agents in food preservatives has also been investigated.The findings of this review illustrated that the phytochemicals present in guava leaves showed excellent antimicrobial and antioxidant properties.Encapsulation appears to be a promising technique for improving the stability,bioavailability,and controlled release of bioactive chemicals extracted from guava leaves.Different methods for the encapsulation of bioactive compounds employed and applied in distinct food systems showed preservation of phytochemicals,improved bioactivities,and nutritional values.Overall,this review paper demonstrated how guava leaves,an abundant source of bioactive compounds,can be used as a multipurpose component to create functional foods,edible coatings,and active packaging to enhance the nutritional,antioxidant,and antimicrobial properties of various food systems that have qualities that promote health.展开更多
Background Unravelling the relationship between trichome density and resistance to jassids in upland cotton,nine parental lines,viz.MCU 5,CO 14,CO 17,TCH 1828,KC 2,KC 3,GISV 323,GTHV 15–34,and RHC 1409 were obtained ...Background Unravelling the relationship between trichome density and resistance to jassids in upland cotton,nine parental lines,viz.MCU 5,CO 14,CO 17,TCH 1828,KC 2,KC 3,GISV 323,GTHV 15–34,and RHC 1409 were obtained from the Tamilnadu Agricultural University.These genotypes were subjected to molecular analysis using 27 primers,merely the JESPR 154 primer amplifying a 150-bp fragment in genotypes exhibiting the pubescence.Result This finding validated the association between pubescence and jassid resistance.Further analysis revealed that resistant genotypes(KC 3,GTHV 15–34,GISV 323,and RHC 1409)exhibited significantly higher trichome densities and length compared with susceptible genotypes.These results stalwartly support the hypothesis that trichomes play a pivotal role in conferring resistance to jassids in upland cotton.Conclusion By breeding cotton varieties with increased trichome density and length,it is possible to reduce jassid infestations,thereby decreasing the reliance on chemical pesticides and promoting a more sustainable agricultural environment.展开更多
Acorn production in oaks(Quercus spp.)shows considerable inter-annual variation,known as masting.The effects of pollen sourced from trees within or outside the stand on acorn production were investigated in pedunculat...Acorn production in oaks(Quercus spp.)shows considerable inter-annual variation,known as masting.The effects of pollen sourced from trees within or outside the stand on acorn production were investigated in pedunculate oak(Quercus robur L.)in an ancient mixed woodland during two moderate masting years.Comparisons were made between natural pollination,hand pollinations with out-of-stand pollen,in-stand pollen or a 1:1 combination of the two pollen sources,and for bagged flowers left unpollinated.After all treatments,>85%of the flowers or developing acorns were aborted between May and August of both years.When flowers were protected with pollen bags and no pollen added,no acorns were produced.In contrast,hand pollination with out-of-stand pollen produced the most acorns both years and significantly more than within-stand pollen or natural pollination in 2022.Hand pollination with out-of-stand or within-stand pollen provided significantly more acorns than natural pollination in 2023.In 2022,hand pollination with a 1:1 mixture of out-of-stand and within-stand pollen yielded an intermediate number of mature acorns between those for the out-of-stand and within-stand pollination treatments.The study provides clear evidence of maternal choice during acorn development in pedunculate oak and of the benefits of pollen supplementation.It also confirms that pedunculate oak is a fruit-maturation masting species;abortion of pollinated flowers and immature acorns determines a mast year(rather than the number of flowers produced)at this site.展开更多
文摘To explore the material basis and mechanisms of the anti-inflammatory effects of Hibiscus mutabilis L..The active ingredients and potential targets of Hibiscus mutabilis L.were obtained through the literature review and SwissADME platform.Genes related to the inflammation were collected using Genecards and OMIM databases,and the intersection genes were submitted on STRING and DAVID websites.Then,the protein interaction network(PPI),gene ontology(GO)and pathway(KEGG)were analyzed.Cytoscape 3.7.2 software was used to construct the“Hibiscus mutabilis L.-active ingredient-target-inflammation”network diagram,and AutoDockTools-1.5.6 software was used for the molecular docking verification.The antiinflammatory effect of Hibiscus mutabilis L.active ingredient was verified by the RAW264.7 inflammatory cell model.The results showed that 11 active components and 94 potential targets,1029 inflammatory targets and 24 intersection targets were obtained from Hibiscus mutabilis L..The key anti-inflammatory active ingredients of Hibiscus mutabilis L.are quercetin,apigenin and luteolin.Its action pathway is mainly related to NF-κB,cancer pathway and TNF signaling pathway.Cell experiments showed that total flavonoids of Hibiscus mutabilis L.could effectively inhibit the expression of tumor necrosis factor(TNF-α),interleukin 8(IL-8)and epidermal growth factor receptor(EGFR)in LPS-induced RAW 264.7 inflammatory cells.It also downregulates the phosphorylation of human nuclear factor ĸB inhibitory protein α(IĸBα)and NF-κB p65 subunit protein(p65).Overall,the anti-inflammatory effect of Hibiscus mutabilis L.is related to many active components,many signal pathways and targets,which provides a theoretical basis for its further development and application.
基金supported by the Joint Funds of the National Natural Science Foundation of China(Grant No.U22A20494)the 1+9 Open Competition Project of Sichuan Academy of Agricultural Sciences(1+9KJGGo02)+4 种基金the National Key R&D Program of China(2024YFA130670O)the“5+1”Agricultural Frontier Technology Research Initiative of Sichuan Academy of Agricultural Sciences(5+1QYGG003)the Project of Sichuan Province Engineering Technology Research Center of Vegetables(2023PZSC0303)the 14th Five-Year Plan Vegetable Breeding Project of Sichuan Province(2021YFYZ0022)the Experts of Sichuan Vegetable Innovation Team(SCCXTD-2025-05).
文摘Radish(Raphanus sativus L.)is an important cruciferous root vegetable,with bolting regulated by multiple genes.However,the genetic mechanisms underlying bolting regulation remain unclear.Here,the genome of the cultivar C60213 is assembled into a high-quality,gap-free telomere-to-telomere structure,spanning nine chromosomes and totaling 472.71 Mb,using a combination of Oxford Nanopore,PacBio,and Hi-C sequencing technologies.It identifies 49,768 protein-coding genes,97.38%of which are functionally annotated.Repetitive sequences constitute 59.72%of the genome,primarily comprising long terminal repeats.A high-density genetic linkage map is constructed using an F2 population derived from a cross between early-and late-bolting radishes,identifying seven major quantitative trait loci associated with bolting and flowering.RNA-seq and quantitative real-time PCR analysis reveal that the RsMIPS3 gene is found to be associated with bolting,with its expression decreasing during this process.Notably,RsMIPS3 overexpression in Arabidopsis delays bolting,confirming its role in regulating bolting time.These findings advance radish genome research and provide a valuable target for breeding late-bolting varieties.
基金supported by the National Key Research and Development Program of China(No.2020YFC1807805)the Science and Technology Planning Project of Guangzhou,Guangdong Province,China(No.202206010176).
文摘Industrial activities have caused widespread arsenic(As)contamination in soil and medicinal crops across south-ern Asia.This study constructed interplanting systems combing medicinal crops with Pteris vittata L.,aiming to mitigate the risk of As exposure in medicinal crops,while simultaneously achieving ecological remediation of contaminated soil.The results revealed that interplanting with P.vittata significantly enhanced the yield of Gynos-temma pentaphyllum by 31.90%(P<0.05)compared with monoculture systems.Under monoculture conditions,the As concentration in G.pentaphyllum leaves reached 2.34 mg/kg,exceeding the national food safety standard(GB2762–2017,2 mg/kg).However,interplanting with P.vittata effectively reduced the As concentration in G.pentaphyllum leaves to 1.82 mg/kg.Furthermore,the interplanting of P.vittata with Rhus chinensis significantly inhibited As translocation from belowground to aboveground tissues in R.chinensis.Compared to monoculture,the stem biomass of P.vittata was significantly increased by 57.50%and 70.32%when interplanted with G.pentaphyllum and Cassia obtusifolia L.(P<0.05).So the As enrichment of P.vittata was enhanced in interplanting systems,which is beneficial for the As removal from contaminated soil.The study demonstrated that interplant-ing primarily regulates plant As uptake through modifications of rhizosphere physicochemical properties and As bioavailability,especially for water-soluble As that is easily absorbed by plants.In conclusion,the interplant-ing models integrating medicinal crops and P.vittata can achieve the goal of“remediating while producing”in As-contaminated soil.
文摘The cultivation of Soledad pepper(Capsicum annuum L.)is essential in Oaxaca and Veracruz,but it faces issues with pests and diseases,which affect yield and cause economic losses.To mitigate these impacts,farmers have started using biostimulants such as chitosan and plant growth promoting bacteria instead of agrochemicals due to their environmental and health benefits.This study evaluated the effect of Bacillus subtilis and chitosan,both individually and combined,on the growth,yield,and fruit quality of Soledad pepper under greenhouse conditions.Four treatments were applied at different stages of the crop cycle:Q(Chitosan),BS(Bacillus subtilis),Q+BS(Chitosan+Bacillus subtilis),and T(Control).The results showed that the combination of chitosan and Bacillus subtilis significantly improved plant growth,especially in height and stem diameter.The chitosan treatment produced the highest number of fruits and plant weight,while the combination of chitosan and Bacillus subtilis enhanced fruit quality,increasing characteristics such as size,weight,pericarp thickness,and physicochemical parameters,notably in brix degrees,citric acid percentage,and pH,outperforming the individual treatments and the control.
文摘Background:Epidemiological studies have confirmed that longer exposure to insecticides like cypermethrin(CYP)significantly increases the risk of male reproductive toxicity.Crocus sativus L.has been recognized due to its therapeutic properties,but its exact role and molecular mechanisms in treatment of reproductive dysfunction remain unclear.Methods:During this study,36 rats were randomly divided into six groups(n=6):control,CYP-induced(60 mg/kg),standard(leuprolide 3 mg/kg)and three treatment groups receiving aqueous,ethanolic,and oil extracts(50 mg/kg or 20 mL/kg)for post-toxicity induction.Results:The finding represented that exposure of CYP significantly increased oxidative stress,disrupted testicular architecture,and markedly reduced testosterone levels(P<0.05).Importantly,Crocus sativus L.treatment alleviated these changes by increasing the expression of Nrf2(nuclear factor erythroid 2-related factor 2),restoring the activity of antioxidant enzymes,and enhancing testicular histomorphology.Surprisingly,molecular docking established a high binding affinity of Crocus sativus L.phytoconstituents such as gallic acid,cinnamic acid and quercetin to the Nrf2-Keap1 complex.It is worth noting that,Crocus sativus L.exhibited a high level of protection against reproductive toxicity caused by CYP in male rats,which was mediated by the activation of Nrf2 pathway,reduction of oxidative damage,and favorable ADMET characteristics.Conclusion:Notably,this research provides a more valid,safe,and effective method of developing new drugs for reproductive disorders,however,further investigation is needed to support the research findings and implement it in clinical practice.
基金supported by the key research project for fig development of Weiyuan County(Grant No.1002-69199007),China.
文摘Dry fig is a traditional healthy snack and has important economic value in a number of Mediterranean and Middle Eastern countries.Cultivars with no anthocyanin accumulation in the fruit peel are preferred for dry fig production.R2R3-MYB transcription factors have promotive or repressive regulatory roles in plant anthocyanin biosynthesis.In this study,113 R2R3-MYB genes were identified in Ficus carica,3 of which were assigned to the S4 subfamily of flavonoid-biosynthesis repressors.FcMYB57 was further recruited as a candidate anthocyaninbiosynthesis repressor based on its sequence features and expression,which was significantly negatively correlated with that of anthocyanin-biosynthesis structural genes.Transient overexpression of FcMYB57 in strawberry totally inhibited fruit pigmentation and significantly increased fruit firmness.The metabolomic analysis confirmed a significant reduction in the contents of cyanidin-3-O-glucoside and pelargonidin-3-O-glucoside,as well as other flavonoids,and transmission electron microscopy revealed an increment in cell-wall thickness.Transcriptome analysis showed downregulation of anthocyanin-biosynthesis structural genes and upregulation of genes related to xylan synthesis.Yeast one-hybrid and dual luciferase assays demonstrated a negative regulatory effect of FcMYB57 on the promoter of FcUFGT3(UDP glucose-flavonoid 3-O-glcosyl-transferase).Yeast two-hybrid assay showed that FcMYB57 does not interact with FcbHLH42,3,14,MYC2,or FcTTG1,all of which have a previously identified or predicted role in flavonoid biosynthesis,however,interaction was detected with FcTPL(Topless),suggesting that FcMYB57 serves as an active repressor of anthocyanin biosynthesis.This is the first identification of an anthocyaninbiosynthesis repressor in fig,with a possible role in fig fruit quality.
基金supported by the National Key R&D Program of China(2016YFD0101900 and 2016YFD0100305)the National Natural Science Foundation of China(Grant No.32001582)the General Project of Natural Science Research in Colleges and Universities of Jiangsu Province(Grant No.20KJB210002)。
文摘Long-chain acyl-CoA synthetase(LACS),belonging to the AMP-bound superfamily,catalyzes the activation of long-chain fatty acids to their coenzyme A esters.Candidate Gene Association analysis showed that LACS8 was not only related to the content of fatty acid,but also,surprisingly,to the content of glucosinolates.Here,we cloned BnaLACS8A03 from rapeseed,and demonstrated its association with the glucosinolates content.We found that BnaLACS8A03 was widely expressed in all tissues and organs,and significantly highly expressed in leaf and flower,which are the sites of glucosinolate synthesis.Bioinformatics predicted that it was located in peroxisome,where theβ-oxidation of fatty acids mainly occurred,indicating that BnaLACS8A03 was involved in the metabolism of fatty acids.Phenotypic studies of RNA interference(RNAi)lines revealed that down-regulation of BnaLACS8A03 in rapeseed significantly reduced the glucosinolates content.In addition,the expression of glucosinolates biosynthesis-related genes and transport-related genes is altered in BnaLACS8A03-RNAi plants,suggesting that a possible crosstalk regulation mechanism exists in the regulation network of glucosinolate mechanism.Our results demonstrate that glucosinolates content can be manipulated by altering the expression of long chain acyl-Coenzyme A synthase gene BnaLACS8A03,which provide an available genetic resource for low glucosinolate breeding practice in rapeseed.
基金support and library facilities in accomplishing this part of the study.
文摘Ceratopteris thalictroides (L.) Brongn (C. thalictroides), a fern from the Pteridaceae family, isan edible species that remains largely underutilized, even though it possesses a diverserange of applications. This review aims to provide a comprehensive overview of itsgeographical distribution, ethnomedicinal applications, phytochemical constituents,pharmacological activities, and ecological importance. Various phytochemical analysesidentified the presence of phenols, flavonoids, alkaloids, terpenoids, and fatty acids from C.thalictroides, responsible for its multiple pharmacological properties, including antioxidant,antimicrobial, anti-inflammatory, anti-diabetic, and anticancer activities. Additionally, itsunique antheridiogen activity makes it a valuable model organism for plant genetics andreproductive studies. This review systematically summarizes the phytopharmacologicalpotential and ecological significance of C. thalictroides. The plant holds promise as a sourceof novel therapeutics and as a sustainable bioresource for pharmaceutical andenvironmental applications.
基金financial support from the State Key Research and Development Plan,China(2022YFD1801105)the 2115 Talent Development Program of China Agricultural University(00109015).
文摘Lablab purpureus(L.)Sweet(L.purpureus)has been reported to alleviate diarrhea,although the precise mechanism remains unclear.This study identified the primary active components of L.purpureus utilizing ultraperformance liquid chromatography/tandem mass spectrometry(UPLC-MS/MS).A rhubarb-induced diarrhea model in rats was utilized to assess the therapeutic efficacy of L.purpureus.Alterations in gut microbiota and fecal metabolism were analyzed via 16S r DNA analysis and targeted metabolomics.Flora elimination and fecal transplantation techniques were employed to deepen understanding of the role of intestinal flora in L.purpureus treatment.The study findings indicated that the main constituents of L.purpureus included trigonelline,piperidinic acid,and L-(–)-malic acid,among others.L.purpureus treatment significantly alleviated all diarrhea symptoms in rats,encompassing reduced fecal water content,weight loss,shortened colon length,diminished histological damage,and decreased inflammatory factors.Furthermore,L.purpureus significantly enhanced the expression of tight junction markers and restored the dysregulated intestinal flora in diarrheic rats by increasing Prevotella and reducing Lactobacillus.Additionally,the production of propionic acid and other short-chain fatty acids(SCFAs)increased in diarrheic rats treated with L.purpureus,suggesting a substantial alteration in the intestinal environment.Crucially,the protective efficacy of L.purpureus diminishes in the absence of gut flora.Subsequent fecal transplantation tests demonstrated that feces from the L.purpureus-treated group alleviated rhubarb-induced diarrhea,emphasizing the pivotal role of gut microbiota in the antidiarrheal efficacy of L.purpureus.In conclusion,our findings elucidate the underlying mechanisms of L.purpureus'antidiarrheal action and its beneficial impact on intestinal microflora.Moreover,these results provide compelling evidence supporting the therapeutic use of L.purpureus for the treatment of diarrhea and its associated complications.
基金Supported by Guangxi Key Laboratory of Zhuang and Yao Medicine(GuiKeJiZi 201432)Zhuang and Yao Medicine Collaborative Innovation Center(GuiJiaoKeYan 201320)+3 种基金Guangxi Young Talent Project of Guangxi University of Chinese Medicine(2022C030)Ethnic Medicine Resources and Application Engineering Research Center in Guangxi Zhuang Autonomous Region(GuiFaGaiGaoJiHan 20202605)Key Discipline Project of Traditional Chinese Medicine in Guangxi—Zhuang Medicine(GZXK-Z-20-64)Guangxi First-class Discipline of Traditional Chinese Medicine—Ethnopharmacology(GuiJiaoKeYan 201812).
文摘[Objectives]To compare the leaf morphology and leaf venation patterns of Hedyotis auricularia L.and its adulterants,such as Mitracarpus hirtus(L.)DC.,Spermacoce pusilla Wall.and Spermacoce alata Aubl.,produced in Guangxi,so as to provide a simple and rapid identification method for the identification of H.auricularia L.in Guangxi.[Methods]LMVP(leaf morphology-venation pattern)identification method was used to study the characteristics of leaf morphology-venation pattern.[Results]Characteristics of leaf morphology-venation pattern:(i)H.auricularia L.:circular knotted-curved pinnate leaf venation without reaching the margin;the primary veins run straight without branches,the number of secondary veins is 5 to 9,and the angles included in tertiary veins are mostly near right angles or obtuse angles.(ii)M.hirtus(L.)DC.:curved pinnate leaf venation without reaching the margin;the primary veins run straight without obvious changes,the number of secondary veins is 3 to 5,and the tertiary veins are slender,and the included angles are mostly acute.(iii)S.pusilla Wall.:curved leaf venation without reaching the leaf margin;there are 4 to 5 pairs of secondary veins,most of which are opposite;the angle between the secondary veins and the primary veins in the middle and near the petiole is mostly medium acute angle,and the extension of the tertiary veins has no fixed direction.(iv)S.alata Aubl.:circular knotted-curved pinnate leaf venation;the primary veins are convex and straight without branches,and there are 4 to 6 pairs of secondary veins,alternating.The angle between secondary veins and primary veins is mostly medium acute angle,the angle between tertiary veins and secondary veins is near right angle,and the tertiary veins are mostly transversely extended.[Conclusions]The leaf morphology-venation patterns of the above-mentioned H.auricularia L.and M.hirtus(L.)DC.,S.pusilla Wall.,and S.alata Aubl.can be used as their identification features,and the identification method has certain operability,which provides an identification idea for the identification of Chinese herbal medicines.
文摘Passiflora incarnata L.,commonly known as passionflower,is traditionally cultivated as an ornamental plant but has demonstrated diverse therapeutic potential.Its pharmacological effects are attributed to bioactive compounds such as flavonoids and alkaloids,which influence multiple biological pathways.This review aims to summarise and critically analyse recent findings on the pharmacological properties of Passiflora incarnata L.,focusing on its neuropsychiatric,antioxidant,antimicrobial,and anticancer activities.A targeted literature search was conducted in PubMed,Scopus,Web of Science,and Google Scholar for peer-reviewed publications between 2000 to 2025.Relevant articles were screened,and a more appropriate article related to the objective of the review was selected.Some classical papers are also cited as per the requirement of the topic.Passiflora incarnata L.showed multifunctional medicinal properties with various applications in neuropsychiatry,oxidative stress management,antimicrobial agent,and as an anticancer agent.The U.S.Food and Drug Administration categorizes passionflower extracts as“generally recognized as safe”.However,most evidence remains preclinical,with methodological variation limiting generalisation.Standardised formulation,robust clinical trials,and in-depth in vivo studies are essential to establish its therapeutic relevance and safety in modern medicine.
基金supported by the National Natural Science Foundation of China (Nos.81973410 and 82473537)the Independent Research Fund of Yunnan Characteristic Plant Extraction Laboratory (Nos.2022YKZY002 and 2022YKZY004)。
文摘Nigella sativa L.seeds have been traditionally utilized in Chinese folk medicine for centuries to treat vitiligo.This study revealed that the ethanolic extract of Nigella sativa L.(HZC)enhances melanogenesis and mitigates oxidative stress-induced cellular senescence and dysfunction in melanocytes.In accordance with established protocols,the ethanol fraction from Nigella sativa L.seeds was extracted,concentrated,and lyophilized to evaluate its herbal effects via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assays,tyrosinase activity evaluation,measurement of cellular melanin contents,scratch assays,senescence-associatedβ-galactosidase(SA-β-gal)staining,enzyme-linked immunosorbent assay(ELISA),and Western blot analysis for expression profiling of experimentally relevant proteins.The results indicated that HZC significantly enhanced tyrosinase activity and melanin content while notably increasing the protein expression levels of Tyr,Mitf,and gp100 in B16F10 cells.Furthermore,HZC effectively mitigated oxidative stress-induced cellular senescence,improved melanocyte condition,and rectified various functional impairments associated with melanocyte dysfunction.These findings suggest that HZC increases melanin synthesis in melanocytes through the activation of the MAPK,PKA,and Wnt signaling pathways.In addition,HZC attenuates oxidative damage induced by H2O2 therapy by activating the nuclear factor E2-related factor 2-antioxidant response element(Nrf2-ARE)pathway and enhancing the activity of downstream antioxidant enzymes,thus preventing premature senescence and dysfunction in melanocytes.
基金Financial support from Beijing Natural Science Foundation(No.7232283)。
文摘From the seeds of Peganum harmala L.,three new alkaloids ofβ-carboline were isolated.Among them,peganumiums A(1)and B(2)were dimers with specific new scaffolds,all with long conjugated systems.Peganumium A and peganumium C(3)were ionic alkaloid salts and peganumium B was a hexacycliccondensed alkaloid.The biosynthetic pathways of the three compounds above were also speculated.A preliminary cytotoxicity assay revealed that peganumium B had strong in vitro antiproliferative ability against a variety of cancer cells.The analysis of^(1)H nuclear magnetic resonance(NMR)metabolomics suggested that the antiproliferative mechanism of peganumium B could be associated with the biosynthesis of phenylalanine,tyrosine and tryptophan,the metabolism of glycine,serine,and threonine,the metabolism of taurine and hypotaurine,and the metabolism of nicotinate and nicotinamide.In addition,peganumium B could reduce the mitochondrial content of body-wall muscle cells of a Caenorhabditis elegans(C.elegans)strain in vivo.
文摘Bentonite is a very useful material for improving soil properties,which enhances the ability of plants to grow and produce in different conditions.The experiment was carried out in an agricultural nursery in one of the areas of the City of Diwaniyah,in a house covered with green netting,with a shade rate of 25%,to study the effect of bentonite and humic acid on the growth and flowering of a Catharanthus roseus L.plant in sandy soil.The experiment included two factors:the first factor was bentonite clay,and the second factor was humic acid.Using a randomized complete block design(R.C.B.D)with three replications,data were analyzed using the analysis of variance(ANOVA)method,and comparison was made according to the least significant difference(L.S.D)test at a probability level of 0.05.The experiment consisted of adding bentonite clay at 0,2,6,and 8 g L-1,humic acid at 0,0.5,1,and 10 g L-1.The results showed that adding bentonite clay and humic acid to sandy soil can have a significant positive effect on the growth and flowering of the Catharanthus roseus plant grown in poor sandy soil conditions.Bentonite,clay and humic acid were added at concentrations of 8 and 10 g L-1,which led to an increase in plant height and number of leaves and leaf area.They reached 30.07,23.84 cm2,76.62,63.42 cm2 for leaf-1 and 24.73,20.22 cm2 for leaf-1,respectively.The results also showed an increase in the content of nitrogen(N),phosphorus(P),and potassium(K)in leaves by 2.27,1.92,1.99%and 1.51,1.22,1.77%.This also led to an increase in chlorophyll pigment and anthocyanin at the highest concentration and gave the highest value.Therefore,adding bentonite and humic acid together gave the highest values in vegetative and chemical characteristics,compared to treatments without addition.
基金supported by the Shennong Laboratory Project of Henan Province,China(SN01-2022-01)the China Postdoctoral Science Foundation(2023M731006)the Project of Science and Technology of Henan Province,China(232102111104)。
文摘One of agriculture’s major challenges is the low efficiency of phosphate(Pi)use,which leads to increased costs,harmful environmental impacts,and the depletion of phosphorus(P)resources.The TaPHT1;6 gene,which encodes a high-affinity Pi transporter(PHT),plays a crucial role in Pi absorption and transport.In this study,the promoter and coding regions of three TaPHT1;6 gene copies on chromosomes 5A,5B,and 5D were individually amplified and sequenced from 167 common wheat(Triticum aestivum L.)cultivars.Sequence analysis revealed 16 allelic variation sites within the promoters of TaPHT1;6-5B among these cultivars,forming three distinct haplotypes:Hap1,Hap2,and Hap3.Field trials were conducted over two years to compare wheat genotypes with these haplotypes,focusing on assessing plant dry weight,grain yield,P content,Pi fertilizer absorption efficiency,and Pi fertilizer utilization efficiency.Results indicated that Hap3 represented the favored Pi-efficient haplotype.Dual-luciferase reporter assay demonstrated that the Hap3 promoter,carrying the identified allelic variation sites,exhibited higher gene-driven capability,leading to increased expression levels of the TaPHT1;6-5B gene.We developed a distributed cleaved amplified polymorphic site marker(dCAPS-571)to distinguish Hap3 from the other two haplotypes based on these allelic variation sites,presenting an opportunity for breeding Pi-efficient wheat cultivars.This study successfully identified polymorphic sites on TaPHT1;6-5B associated with Pi efficiency and developed a functional molecular marker to facilitate future breeding endeavors.
基金Supported by Guangxi Science Foundation(0639027)Science and Technology Developmental Fund of Guangxi Academy of Agricultural Sciences(2006025)~~
文摘[Objective] The research aimed to lay a foundation for the screening of cell lines producing secondary metabolites of Brucea javanica(L.)Merr.[Method] The insecticidal activities of the extracts from branch and 3 different types of calluses of Brucea javanica(L.)Merr.was detected through methods of leaf disc and potted seedlings against the diamond back moth.[Result] Extracts from four kinds of Brucea javanica(L.)Merr.tissues assumed both the activities of antifeedant and oviposition deterrency against the diamond back moth.Antifeedant effect of extracts was in turn the callus C< callus B< callus A< branches.Oviposition deterrency activity of the extracts was in turn the callus A> branch > callus B>callus C.The insecticidal activities of callus A and B were higher than that of the callus C.[Conclusion] The results show that insecticidal activity of callus and its growth rate is inversely proportional.
文摘The aim of the present study was to evaluate the effects of baobab seed washing and origin on the chemical composition of the oil extracted by pressing. Six (6) oil samples were obtained from seeds of three (3) geographical origins. The identification and quantification of fatty acids and the polyphenolic profile were carried out by GC-MS and HPLC-UV, respectively. Analysis of fatty acid methyl esters allowed the identification and quantification of 18 fatty acids. Oils from unwashed seeds were richer in palmitic (C16:0), stearic (C18:0), oleic (C18:1) and arachidic (C20:0) acids. In addition, HPLC-UV analysis at 279 nm shows that oils from unwashed seeds are richer in tyrosol, hydroxytyrosol and caffeic acid. With regard to the polyphenolic profile, gallic acid and quercetin were not detected in these baobab oils. Principal component analysis of fatty acid and phenolic compound content showed that oils from unwashed seeds would best preserve their chemical and associated potential bioactive characteristics.
文摘Guava(Psidium guajava L.),a plant belonging to the Myrtaceae family,holds significant medicinal and nutritional values.Leaves of the plant are described to be elliptical with a dark green color and are utilized in the treatment of gastrointestinal and respiratory issues along with increasing the platelet count in patients suffering from dengue.In this review paper,the nutritional composition and bioactive compounds of guava leaves,including proteins,vitamins,minerals,and polysaccharides,have been reviewed.The methods of extraction of bioactive compounds from guava leaves and their bioactivities,including antioxidant,anti-diabetic,and anti-cancer potential,have been explored.Further,encapsulation techniques for improving the transport of bioactive compounds and living cells into foods have been studied.The application of guava leaves in different food systems such as herbal tea,pork sausage,chocolates,jelly,meals for chickens,functional beverages,and as antibacterial agents in food preservatives has also been investigated.The findings of this review illustrated that the phytochemicals present in guava leaves showed excellent antimicrobial and antioxidant properties.Encapsulation appears to be a promising technique for improving the stability,bioavailability,and controlled release of bioactive chemicals extracted from guava leaves.Different methods for the encapsulation of bioactive compounds employed and applied in distinct food systems showed preservation of phytochemicals,improved bioactivities,and nutritional values.Overall,this review paper demonstrated how guava leaves,an abundant source of bioactive compounds,can be used as a multipurpose component to create functional foods,edible coatings,and active packaging to enhance the nutritional,antioxidant,and antimicrobial properties of various food systems that have qualities that promote health.
文摘Background Unravelling the relationship between trichome density and resistance to jassids in upland cotton,nine parental lines,viz.MCU 5,CO 14,CO 17,TCH 1828,KC 2,KC 3,GISV 323,GTHV 15–34,and RHC 1409 were obtained from the Tamilnadu Agricultural University.These genotypes were subjected to molecular analysis using 27 primers,merely the JESPR 154 primer amplifying a 150-bp fragment in genotypes exhibiting the pubescence.Result This finding validated the association between pubescence and jassid resistance.Further analysis revealed that resistant genotypes(KC 3,GTHV 15–34,GISV 323,and RHC 1409)exhibited significantly higher trichome densities and length compared with susceptible genotypes.These results stalwartly support the hypothesis that trichomes play a pivotal role in conferring resistance to jassids in upland cotton.Conclusion By breeding cotton varieties with increased trichome density and length,it is possible to reduce jassid infestations,thereby decreasing the reliance on chemical pesticides and promoting a more sustainable agricultural environment.
基金supported by Future Trees TrustThe Patsy Wood Trust+2 种基金Scottish Forestry TrustAitchinson Tait TrustAction Oak
文摘Acorn production in oaks(Quercus spp.)shows considerable inter-annual variation,known as masting.The effects of pollen sourced from trees within or outside the stand on acorn production were investigated in pedunculate oak(Quercus robur L.)in an ancient mixed woodland during two moderate masting years.Comparisons were made between natural pollination,hand pollinations with out-of-stand pollen,in-stand pollen or a 1:1 combination of the two pollen sources,and for bagged flowers left unpollinated.After all treatments,>85%of the flowers or developing acorns were aborted between May and August of both years.When flowers were protected with pollen bags and no pollen added,no acorns were produced.In contrast,hand pollination with out-of-stand pollen produced the most acorns both years and significantly more than within-stand pollen or natural pollination in 2022.Hand pollination with out-of-stand or within-stand pollen provided significantly more acorns than natural pollination in 2023.In 2022,hand pollination with a 1:1 mixture of out-of-stand and within-stand pollen yielded an intermediate number of mature acorns between those for the out-of-stand and within-stand pollination treatments.The study provides clear evidence of maternal choice during acorn development in pedunculate oak and of the benefits of pollen supplementation.It also confirms that pedunculate oak is a fruit-maturation masting species;abortion of pollinated flowers and immature acorns determines a mast year(rather than the number of flowers produced)at this site.
