Ⅰ. INTRODUCTIONTrypanosoma evansi has the widest geographic range of all the pathogenic trypanosomes and is an important etiological agent of disease in the domestic animals, especially in buffalo and horse in China....Ⅰ. INTRODUCTIONTrypanosoma evansi has the widest geographic range of all the pathogenic trypanosomes and is an important etiological agent of disease in the domestic animals, especially in buffalo and horse in China. However, we have much less knowledge about T. evansi than T. brucei which is spread by tsetse fly in Africa. T. evansi and T. brucei are closely related to and morphologically indistinguishable from each other, so it is necessary to study them by com-展开更多
基金Project supported by the National Natural Science Foundation of China.
文摘Ⅰ. INTRODUCTIONTrypanosoma evansi has the widest geographic range of all the pathogenic trypanosomes and is an important etiological agent of disease in the domestic animals, especially in buffalo and horse in China. However, we have much less knowledge about T. evansi than T. brucei which is spread by tsetse fly in Africa. T. evansi and T. brucei are closely related to and morphologically indistinguishable from each other, so it is necessary to study them by com-
文摘从养殖病死三疣梭子蟹肝胰脏及心脏血淋巴液中分离到大量优势生长的细菌,人工感染试验证明分离菌对三疣梭子蟹有较强的致病性;对分离菌进行了形态特征、理化特性、胞外酶及溶血素活性等表型生物学性状及16S rRNA和gyrB两种基因的分子鉴定。菌株(JGX080708-1)所扩增的16S rRNA基因序列长度为1453bp(GenBank登录号:FJ824663),所扩增的gyrB基因序列长度为1191bp(GenBank登录号:GQ372985);两种基因序列在NCBI通过blast检索,结果均与弧菌属细菌的基因序列自然聚类;根据分离菌的表型及分子特征,判定分离菌为弧菌属(Vibrio Pacini1954)的副溶血弧菌[Vibrio parahaemolyticus(Fujino et al1951)Sakazaki Nakamura and Takizawa1963]。分离菌的药敏试验结果显示,对供试49种抗菌药物中的安曲南等42种药物高度敏感,对林可霉素敏感,对青霉素G等6种药物耐药。
