For monomer reactivity ratios study, the copolymerization of D,L-3-methylglycolide (MG) with glycolide (GA) or D,L-lactide (LA) was carried out in bulk to a certain low conversion in the presence of stannous octoate a...For monomer reactivity ratios study, the copolymerization of D,L-3-methylglycolide (MG) with glycolide (GA) or D,L-lactide (LA) was carried out in bulk to a certain low conversion in the presence of stannous octoate at 140 degrees C. The copolymer compositions were determined by H-1 NMR spectroscopy. The monomer reactivity ratios were evaluated by Fineman-Ross method, Kelen-Tudos method and linear least-squares method. The monomer reactivity ratios of D,L-3-methylglycolide and glycolide or D,L-lactide are r(mg)= 0.73, r(ga)= 1.47; r(mg)= 1.71, r(la)= 0.92, respectively.展开更多
[Objective] The aim of the study is to clone and analyze the gene encoding 14-3-3 protein from banana. [Method] Combined with PCR amplification, RACE (rapid amplification of cDNA ends) technique was employed to clone ...[Objective] The aim of the study is to clone and analyze the gene encoding 14-3-3 protein from banana. [Method] Combined with PCR amplification, RACE (rapid amplification of cDNA ends) technique was employed to clone 14-3-3 gene from banana; then the amplified sequence was sequenced and homologically analyzed. [Result] A new cDNA homologous with 14-3-3 protein genes were obtained by RT-PCR and RACE ( rapid amplification of cDNA ends ) approaches. The full length of this cDNA was 866 bp encoding 197 amino acids. Alignment of deduced amino acid sequence with those from other plants revealed that the cDNA shared high homology with 14-3-3 protein genes from other plants, and was designated as Musa acuminata 14-3-3 gene (Ma-14-3-3d). Phylogenetic analysis reveals that Ma-14-3-3d has closer genetic relationship with those from monocotyledon species than those from other species. [Conclusion] Ma-14-3-3d belongs to the same lineage of 14-3-3 from monocotyledon.展开更多
目的利用3D景深合成技术、显微偏光技术对茜草及其易混淆品进行鉴别,建立一种快速鉴别茜草的方法并提供相应的鉴别特征高清图。方法采用3D景深合成技术对茜草及其混淆品的表面及断面进行鉴别,并拍摄其微形态特征图。通过徒手切片法制作...目的利用3D景深合成技术、显微偏光技术对茜草及其易混淆品进行鉴别,建立一种快速鉴别茜草的方法并提供相应的鉴别特征高清图。方法采用3D景深合成技术对茜草及其混淆品的表面及断面进行鉴别,并拍摄其微形态特征图。通过徒手切片法制作其临时装片,并利用偏光技术对其针晶束及其它组织结构进行观察、鉴别,拍摄其正常光与偏光下的横切面特征高清图。结果茜草的混淆品主要有茜草茎埋在地下部分、大茜草Rubia magna P.G.Xiao、蓬子菜Galium verum L.两种,可以通过其表面的粗糙程度、木栓层细胞的形态、针晶束的密度以及木质部、木栓层、韧皮部的径向比例进行区分。结论采用3D景深合成技术、偏光技术能够对茜草及其混淆品进行区分,为茜草的鉴别提供了新的思路。该方法操作简便、制作快速、鉴别特征明显,非常适合现场快检。展开更多
目的探讨复杂胫骨平台骨折患者应用3D打印技术辅助改良后内侧倒L入路切开复位内固定术治疗的临床疗效及安全性。方法 41例复杂胫骨平台骨折患者根据治疗方式分为3D组18例和常规组23例,2组均行改良后内侧倒L入路切开复位内固定术。常规...目的探讨复杂胫骨平台骨折患者应用3D打印技术辅助改良后内侧倒L入路切开复位内固定术治疗的临床疗效及安全性。方法 41例复杂胫骨平台骨折患者根据治疗方式分为3D组18例和常规组23例,2组均行改良后内侧倒L入路切开复位内固定术。常规组术前行膝关节CT平扫,3D组在常规组基础上采用3D技术打印实体模型并在实体模型上模拟手术。比较2组手术时间、术中出血量、手术并发症发生率,以及术后第3天胫骨平台骨折复位放射学Rasmussen评分,术后12个月膝关节功能(hospital for special surgery,HSS)评分。结果 3D组手术时间[(127.3±11.7)min]较常规组[(167.4±15.8)min]短,术中出血量[(211.7±18.7)mL]较常规组[(291.6±25.3)mL]少,手术并发症发生率(5.55%)较常规组(26.08%)低,术后第3天Rasmussen评分及术后12个月HSS评分[(15.2±1.9)、(84.3±11.2)分]较常规组[(9.9±2.1)、(71.0±5.7)分]高(P<0.05)。结论复杂胫骨平台骨折患者行3D打印技术辅助改良后内侧倒L入路切开复位内固定术可缩短手术时间,减少术中出血量,提高骨折复位质量,改善膝关节功能,且并发症发生率低。展开更多
基金This work was supported by the Key Project of the National Natural Science Foundation of China!(59833 140).
文摘For monomer reactivity ratios study, the copolymerization of D,L-3-methylglycolide (MG) with glycolide (GA) or D,L-lactide (LA) was carried out in bulk to a certain low conversion in the presence of stannous octoate at 140 degrees C. The copolymer compositions were determined by H-1 NMR spectroscopy. The monomer reactivity ratios were evaluated by Fineman-Ross method, Kelen-Tudos method and linear least-squares method. The monomer reactivity ratios of D,L-3-methylglycolide and glycolide or D,L-lactide are r(mg)= 0.73, r(ga)= 1.47; r(mg)= 1.71, r(la)= 0.92, respectively.
文摘[Objective] The aim of the study is to clone and analyze the gene encoding 14-3-3 protein from banana. [Method] Combined with PCR amplification, RACE (rapid amplification of cDNA ends) technique was employed to clone 14-3-3 gene from banana; then the amplified sequence was sequenced and homologically analyzed. [Result] A new cDNA homologous with 14-3-3 protein genes were obtained by RT-PCR and RACE ( rapid amplification of cDNA ends ) approaches. The full length of this cDNA was 866 bp encoding 197 amino acids. Alignment of deduced amino acid sequence with those from other plants revealed that the cDNA shared high homology with 14-3-3 protein genes from other plants, and was designated as Musa acuminata 14-3-3 gene (Ma-14-3-3d). Phylogenetic analysis reveals that Ma-14-3-3d has closer genetic relationship with those from monocotyledon species than those from other species. [Conclusion] Ma-14-3-3d belongs to the same lineage of 14-3-3 from monocotyledon.
文摘目的利用3D景深合成技术、显微偏光技术对茜草及其易混淆品进行鉴别,建立一种快速鉴别茜草的方法并提供相应的鉴别特征高清图。方法采用3D景深合成技术对茜草及其混淆品的表面及断面进行鉴别,并拍摄其微形态特征图。通过徒手切片法制作其临时装片,并利用偏光技术对其针晶束及其它组织结构进行观察、鉴别,拍摄其正常光与偏光下的横切面特征高清图。结果茜草的混淆品主要有茜草茎埋在地下部分、大茜草Rubia magna P.G.Xiao、蓬子菜Galium verum L.两种,可以通过其表面的粗糙程度、木栓层细胞的形态、针晶束的密度以及木质部、木栓层、韧皮部的径向比例进行区分。结论采用3D景深合成技术、偏光技术能够对茜草及其混淆品进行区分,为茜草的鉴别提供了新的思路。该方法操作简便、制作快速、鉴别特征明显,非常适合现场快检。
文摘目的探讨复杂胫骨平台骨折患者应用3D打印技术辅助改良后内侧倒L入路切开复位内固定术治疗的临床疗效及安全性。方法 41例复杂胫骨平台骨折患者根据治疗方式分为3D组18例和常规组23例,2组均行改良后内侧倒L入路切开复位内固定术。常规组术前行膝关节CT平扫,3D组在常规组基础上采用3D技术打印实体模型并在实体模型上模拟手术。比较2组手术时间、术中出血量、手术并发症发生率,以及术后第3天胫骨平台骨折复位放射学Rasmussen评分,术后12个月膝关节功能(hospital for special surgery,HSS)评分。结果 3D组手术时间[(127.3±11.7)min]较常规组[(167.4±15.8)min]短,术中出血量[(211.7±18.7)mL]较常规组[(291.6±25.3)mL]少,手术并发症发生率(5.55%)较常规组(26.08%)低,术后第3天Rasmussen评分及术后12个月HSS评分[(15.2±1.9)、(84.3±11.2)分]较常规组[(9.9±2.1)、(71.0±5.7)分]高(P<0.05)。结论复杂胫骨平台骨折患者行3D打印技术辅助改良后内侧倒L入路切开复位内固定术可缩短手术时间,减少术中出血量,提高骨折复位质量,改善膝关节功能,且并发症发生率低。
