Extracellular polymeric substances(EPS)are present externally to the microorganisms and play an important role in attachment and biofilm formation.These polymers possess antibacterial and antifouling activities.In thi...Extracellular polymeric substances(EPS)are present externally to the microorganisms and play an important role in attachment and biofilm formation.These polymers possess antibacterial and antifouling activities.In this study,the antifouling activity of EPS produced by an epibiotic bacterium associated with macroalga Ulva lactuca was assessed against fouling bacteria and barnacle larvae.Results indicate that the EPS isolated from the epibiotic bacterium inhibits the biofilm formation of the bacteria without much antibacterial activity.Also,the EPS reduced the settlement of barnacle larvae on the hard substrate under laboratory conditions.The epibiotic bacterium was identified as Kocuria flava based on 16 S rRNA gene sequencing.The EPS was further analysed using Fourier transform infrared(FT-IR),nuclear magnetic resonance(NMR)and X-ray diffraction(XRD)to understand the biochemical composition.NMR analysis revealed the presence of polysaccharides,proteins,acetyl amine and succinyl groups.Scanning electron microscope analysis indicated that the EPS consisted of aggregated and irregular sphere-shaped particles.展开更多
从甘蔗渣滤泥中筛得一株具有漆酶活性的细菌SYBC star X,通过16S r RNA基因序列分析,并结合生理生化实验及形态学观察,鉴定为考克氏菌属(Kocuria);利用液态发酵法研究了碳源、氮源、金属离子对其发酵产漆酶的影响,确定菌株SYBC star X...从甘蔗渣滤泥中筛得一株具有漆酶活性的细菌SYBC star X,通过16S r RNA基因序列分析,并结合生理生化实验及形态学观察,鉴定为考克氏菌属(Kocuria);利用液态发酵法研究了碳源、氮源、金属离子对其发酵产漆酶的影响,确定菌株SYBC star X的最优碳源为15 g/Lα-乳糖,最佳氮源为40 g/L大豆蛋白胨,最适铜离子和锰离子的浓度分别为3 mmol/L和0.4 mmol/L。展开更多
BACKGROUND Adult retropharyngeal abscess(RPA)is extremely rare,and most cases reported in the literature were related to tuberculous infection.We present a case of RPA with acute airway obstruction as the main manifes...BACKGROUND Adult retropharyngeal abscess(RPA)is extremely rare,and most cases reported in the literature were related to tuberculous infection.We present a case of RPA with acute airway obstruction as the main manifestation in a 66-year-old woman that was considered to be non-tubercular suppurative inflammation in the retropharyngeal space.CASE SUMMARY A 66-year-old woman complaining of chills and fever was admitted to our hospital.She was initially diagnosed with an acute upper respiratory tract infection.She lost consciousness twice during hospitalization.She regained consciousness immediately upon emergency tracheal intubation.Acute upper airway obstruction was suspected as arterial blood gas analysis showed obvious acute retention of carbon dioxide before the second tracheal intubation.The diagnosis of RPA was confirmed by computed tomography and magnetic resonance imaging.Kocuria kristinae was isolated from blood samples taken from both hands.The patient recovered and was subsequently discharged after receiving antibiotic therapy together with surgical incision and drainage of the abscess.CONCLUSION Clinicians should be alert to the possibility of RPA in patients with acute airway obstruction.Surgical incision and drainage is an effective treatment for RPA.展开更多
Ixodid ticks play an important role in the transmission of a variety of zoonoses of viral, bacterial and protozoan origin, and they also harbor a wealth of microorganisms. To gain more detailed insights into the poten...Ixodid ticks play an important role in the transmission of a variety of zoonoses of viral, bacterial and protozoan origin, and they also harbor a wealth of microorganisms. To gain more detailed insights into the potential interactions between bacterial flora and tick-borne pathogens, we investigated the midgut bacterial flora of laboratory-reared Haemaphysalis longicornis, Hyalomma asiaticum and Rhipicephalus haemaphysaloides. Based on morphological, biochemical, and 16 S rDNA sequencing results, we identified 15 species belonging to 12 genera in the midgut of the three ticks. The bacterial communities were similar to those found in other studies of hematophagous arthropods. Kocuria sp. was the most frequently isolated species and its 16 S rDNA gene sequence was very similar to Kocuria koreensis P31 T. To our knowledge, this is the first report of the bacterial flora of tick midguts and the results show that there were many different bacterial species in each tick species. Among the most common genera, there may have been a novel species in the genus Kocuria. The results might be the first step for looking for different aspects of the pathogen and tick interaction.展开更多
通过16S r RNA序列分析,结合微生物的形态学观察、生理生化试验对甘蔗糖厂成糖工序生产线检出的部分细菌进行了分离鉴定。结果显示,从甘蔗糖生产线样品中获得常见的4株菌,菌株S-1鉴定为克氏库克菌(Kocuria kristinae)、菌株S-2为蜡样芽...通过16S r RNA序列分析,结合微生物的形态学观察、生理生化试验对甘蔗糖厂成糖工序生产线检出的部分细菌进行了分离鉴定。结果显示,从甘蔗糖生产线样品中获得常见的4株菌,菌株S-1鉴定为克氏库克菌(Kocuria kristinae)、菌株S-2为蜡样芽胞杆菌(Bacillus cereus)、菌株S-3为解淀粉芽孢杆菌(Bacillus amyloliquefaciens)、菌株S-4为枯草芽孢杆菌(Bacillus subtilis)。因此,糖厂应该加强微生物污染源和污染途径的监控,以此保证甘蔗糖品质。展开更多
对某番茄酱生产企业提供的辐照杀菌（辐照剂量≥15 k Gy）的5 L无菌袋进行菌落计数、分离和鉴定。3 L无菌水反复漂洗,滤膜过滤后进行平板菌落计数,分离纯化后采用16S r DNA和dna J序列分析分别鉴定到属和种。结果表明,5批次的样品中均...对某番茄酱生产企业提供的辐照杀菌（辐照剂量≥15 k Gy）的5 L无菌袋进行菌落计数、分离和鉴定。3 L无菌水反复漂洗,滤膜过滤后进行平板菌落计数,分离纯化后采用16S r DNA和dna J序列分析分别鉴定到属和种。结果表明,5批次的样品中均有细菌检出,数量在5-132 CFU/袋,9个典型菌落经过16S rDNA序列分析后,分别属6个属,其中栖水菌属（Enhydrobacter）、不动杆菌属（Acinetobacter）和考克斯菌属（Kocuria）各1株,微球菌属（Micrococcus）、微杆菌属（Microbacterium）和芽孢杆菌属（Bacillus）各2株;dna J序列分析则将一株葡萄球菌C8-3鉴定到种-表皮葡萄球菌（Staphylococcus epidermidis）。辐照杀菌的无菌袋虽然微生物数量不高,但是依然残留了一些耐辐照的细菌,它们或多或少会给内装食品带来一定的食品安全隐患或影响食品保质期。展开更多
目的对雾霾中分离的库克细菌进行种属鉴定和药敏实验,研究环境中库克菌属细菌的分布状态、生化特性和耐药特征。方法于2016年冬季雾霾环境下使用空气撞击法收集距地面10~15 m的空气微生物,将分离的细菌纯化培养后进行革兰染色、16S r DN...目的对雾霾中分离的库克细菌进行种属鉴定和药敏实验,研究环境中库克菌属细菌的分布状态、生化特性和耐药特征。方法于2016年冬季雾霾环境下使用空气撞击法收集距地面10~15 m的空气微生物,将分离的细菌纯化培养后进行革兰染色、16S r DNA序列分析、生化鉴定和耐药性分析。结果 8株细菌经16S r DNA测序分析确定为库克菌属细菌,为革兰阳性球菌;经VITEK 2革兰阳性细菌鉴定卡分析,8株菌鉴定为玫瑰色库克菌;其对青霉素类、碳青霉烯类、氨基糖苷类、丙酰胺类、四环素类、安沙霉素类等6类药物有不同程度的敏感,个别菌株对氟喹诺酮类、头孢类、大环内酯类、林可霉素类药物的耐药性表现为中介,未发现耐药株。结论雾霾空气中存在少量的条件致病菌玫瑰色库克菌,该菌对大部分抗生素敏感。展开更多
基金The Deanship of Scientific Research of King Abdulaziz University under contract No.G-153-150-39。
文摘Extracellular polymeric substances(EPS)are present externally to the microorganisms and play an important role in attachment and biofilm formation.These polymers possess antibacterial and antifouling activities.In this study,the antifouling activity of EPS produced by an epibiotic bacterium associated with macroalga Ulva lactuca was assessed against fouling bacteria and barnacle larvae.Results indicate that the EPS isolated from the epibiotic bacterium inhibits the biofilm formation of the bacteria without much antibacterial activity.Also,the EPS reduced the settlement of barnacle larvae on the hard substrate under laboratory conditions.The epibiotic bacterium was identified as Kocuria flava based on 16 S rRNA gene sequencing.The EPS was further analysed using Fourier transform infrared(FT-IR),nuclear magnetic resonance(NMR)and X-ray diffraction(XRD)to understand the biochemical composition.NMR analysis revealed the presence of polysaccharides,proteins,acetyl amine and succinyl groups.Scanning electron microscope analysis indicated that the EPS consisted of aggregated and irregular sphere-shaped particles.
文摘从甘蔗渣滤泥中筛得一株具有漆酶活性的细菌SYBC star X,通过16S r RNA基因序列分析,并结合生理生化实验及形态学观察,鉴定为考克氏菌属(Kocuria);利用液态发酵法研究了碳源、氮源、金属离子对其发酵产漆酶的影响,确定菌株SYBC star X的最优碳源为15 g/Lα-乳糖,最佳氮源为40 g/L大豆蛋白胨,最适铜离子和锰离子的浓度分别为3 mmol/L和0.4 mmol/L。
文摘BACKGROUND Adult retropharyngeal abscess(RPA)is extremely rare,and most cases reported in the literature were related to tuberculous infection.We present a case of RPA with acute airway obstruction as the main manifestation in a 66-year-old woman that was considered to be non-tubercular suppurative inflammation in the retropharyngeal space.CASE SUMMARY A 66-year-old woman complaining of chills and fever was admitted to our hospital.She was initially diagnosed with an acute upper respiratory tract infection.She lost consciousness twice during hospitalization.She regained consciousness immediately upon emergency tracheal intubation.Acute upper airway obstruction was suspected as arterial blood gas analysis showed obvious acute retention of carbon dioxide before the second tracheal intubation.The diagnosis of RPA was confirmed by computed tomography and magnetic resonance imaging.Kocuria kristinae was isolated from blood samples taken from both hands.The patient recovered and was subsequently discharged after receiving antibiotic therapy together with surgical incision and drainage of the abscess.CONCLUSION Clinicians should be alert to the possibility of RPA in patients with acute airway obstruction.Surgical incision and drainage is an effective treatment for RPA.
基金supported by grants from the Special Fund for National Commonweal Institute of China (2012JB12)
文摘Ixodid ticks play an important role in the transmission of a variety of zoonoses of viral, bacterial and protozoan origin, and they also harbor a wealth of microorganisms. To gain more detailed insights into the potential interactions between bacterial flora and tick-borne pathogens, we investigated the midgut bacterial flora of laboratory-reared Haemaphysalis longicornis, Hyalomma asiaticum and Rhipicephalus haemaphysaloides. Based on morphological, biochemical, and 16 S rDNA sequencing results, we identified 15 species belonging to 12 genera in the midgut of the three ticks. The bacterial communities were similar to those found in other studies of hematophagous arthropods. Kocuria sp. was the most frequently isolated species and its 16 S rDNA gene sequence was very similar to Kocuria koreensis P31 T. To our knowledge, this is the first report of the bacterial flora of tick midguts and the results show that there were many different bacterial species in each tick species. Among the most common genera, there may have been a novel species in the genus Kocuria. The results might be the first step for looking for different aspects of the pathogen and tick interaction.
文摘目的对雾霾中分离的库克细菌进行种属鉴定和药敏实验,研究环境中库克菌属细菌的分布状态、生化特性和耐药特征。方法于2016年冬季雾霾环境下使用空气撞击法收集距地面10~15 m的空气微生物,将分离的细菌纯化培养后进行革兰染色、16S r DNA序列分析、生化鉴定和耐药性分析。结果 8株细菌经16S r DNA测序分析确定为库克菌属细菌,为革兰阳性球菌;经VITEK 2革兰阳性细菌鉴定卡分析,8株菌鉴定为玫瑰色库克菌;其对青霉素类、碳青霉烯类、氨基糖苷类、丙酰胺类、四环素类、安沙霉素类等6类药物有不同程度的敏感,个别菌株对氟喹诺酮类、头孢类、大环内酯类、林可霉素类药物的耐药性表现为中介,未发现耐药株。结论雾霾空气中存在少量的条件致病菌玫瑰色库克菌,该菌对大部分抗生素敏感。
