白凤菜含有丰富的矿质元素、氨基酸和锌,在许多地区颇受欢迎,是公认的保健蔬菜,具有一定的药用和食用价值。本研究利用Illumina HiSeq X Ten技术平台对白凤菜进行了转录组测序,并与生化提取、q RT-PCR结果进行交叉验证。结果发现白凤菜...白凤菜含有丰富的矿质元素、氨基酸和锌,在许多地区颇受欢迎,是公认的保健蔬菜,具有一定的药用和食用价值。本研究利用Illumina HiSeq X Ten技术平台对白凤菜进行了转录组测序,并与生化提取、q RT-PCR结果进行交叉验证。结果发现白凤菜一年生长期叶片中的总黄酮含量显著高于半年生长期叶片中的总黄酮含量;转录组结果则显示在一年生长期叶片中,与黄酮生物合成相关的基因中共有10个基因显著上调表达;利用qRT-PCR深入挖掘在总黄酮上调表达路径中的关键基因,发现一年生长期叶片中无色花青素双加氧酶/花青素合成酶(LDOX/ANS)与类黄酮-3’-羟化酶(F3'H)呈显著上调表达。综上,生化提取与转录组数据均显示,一年生长期叶片中总黄酮含量高于半年期叶片,且LDOX/ANS与F3'H在其中起关键调控作用。本研究结果为白凤菜分子生物学研究奠定基础,为白凤菜药用活性成分的生物合成调控、品质改良、品种选育等提供参考依据。展开更多
BACKGROUND Atractylodes japonica Koidz.ex Kitam.(A.japonica,Chinese name:Guan-Cangzhu,Japanese name:Byaku-jutsu),a perennial herb,which is mainly distributed in northeast area of China,it’s often used to treat digest...BACKGROUND Atractylodes japonica Koidz.ex Kitam.(A.japonica,Chinese name:Guan-Cangzhu,Japanese name:Byaku-jutsu),a perennial herb,which is mainly distributed in northeast area of China,it’s often used to treat digestive system diseases such as gastric ulcer(GU).However,the mechanism of its potential protective effects against GU remains unclear.AIM To investigate the protective effects of A.japonica on acetic acid-induced GU rats.METHODS The chemical constituents of A.japonica were determined by ultra performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS)analysis.The rat model of GU was simulated by acetic acid method.The pathological changes of gastric tissues were evaluated by hematoxylin-eosin stain,the levels of epidermal growth factor(EGF),EGF receptor(EGFR),nuclear factor kappa-B(NF-κB),interleukin-1β(IL-1β),IL-10,Na^(+)-K^(+)-ATPase(NKA)in serum and gastric tissues were determined by enzyme-linked immunosorbent assay,and the mRNA expressions of EGFR,NF-κBp65,IkappaBalpha(IκBα)and Zonula Occludens-1(ZO-1)in gastric tissues were determined by real-time reverse transcription polymerase chain reaction,and the efficacy was observed.Then,plasma metabolomic analysis was performed by UPLC-MS/MS to screen the specific potential biomarkers,metabolic pathways and to explore the possible mechani-sms.RESULTS 48 chemical constituents were identified.Many of them have strong pharmacological activity,the results also revealed that A.japonica significantly improved the pathological damage of gastric tissues,increased the expression levels of IL-10,IκBαrelated to anti-inflammatory factors,decreased the expression levels of IL-1β,NF-κB,NF-κBp65,related to proinflammatory factors,restored the levels of factors about EGF,EGFR,ZO-1 associated with ulcer healing and the levels of factors about NKA associated with energy metabolism.Metabolomic analysis identified 10 potential differential metabolites and enriched 7 related metabolic pathways.CONCLUSION These findings contribute to the understanding of the potential mechanism of A.japonica to improve acetic acidinduced GU,and will be of great importance for the development and clinical application of natural drugs related to A.japonica.展开更多
文摘白凤菜含有丰富的矿质元素、氨基酸和锌,在许多地区颇受欢迎,是公认的保健蔬菜,具有一定的药用和食用价值。本研究利用Illumina HiSeq X Ten技术平台对白凤菜进行了转录组测序,并与生化提取、q RT-PCR结果进行交叉验证。结果发现白凤菜一年生长期叶片中的总黄酮含量显著高于半年生长期叶片中的总黄酮含量;转录组结果则显示在一年生长期叶片中,与黄酮生物合成相关的基因中共有10个基因显著上调表达;利用qRT-PCR深入挖掘在总黄酮上调表达路径中的关键基因,发现一年生长期叶片中无色花青素双加氧酶/花青素合成酶(LDOX/ANS)与类黄酮-3’-羟化酶(F3'H)呈显著上调表达。综上,生化提取与转录组数据均显示,一年生长期叶片中总黄酮含量高于半年期叶片,且LDOX/ANS与F3'H在其中起关键调控作用。本研究结果为白凤菜分子生物学研究奠定基础,为白凤菜药用活性成分的生物合成调控、品质改良、品种选育等提供参考依据。
基金Supported by National Natural Science Foundation of China,No.81973478Liaoning Revitalization Talents Program,China,No.XLYC2002004Natural Science Foundation of Liaoning Province,China,No.2019-ZD-0443.
文摘BACKGROUND Atractylodes japonica Koidz.ex Kitam.(A.japonica,Chinese name:Guan-Cangzhu,Japanese name:Byaku-jutsu),a perennial herb,which is mainly distributed in northeast area of China,it’s often used to treat digestive system diseases such as gastric ulcer(GU).However,the mechanism of its potential protective effects against GU remains unclear.AIM To investigate the protective effects of A.japonica on acetic acid-induced GU rats.METHODS The chemical constituents of A.japonica were determined by ultra performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS)analysis.The rat model of GU was simulated by acetic acid method.The pathological changes of gastric tissues were evaluated by hematoxylin-eosin stain,the levels of epidermal growth factor(EGF),EGF receptor(EGFR),nuclear factor kappa-B(NF-κB),interleukin-1β(IL-1β),IL-10,Na^(+)-K^(+)-ATPase(NKA)in serum and gastric tissues were determined by enzyme-linked immunosorbent assay,and the mRNA expressions of EGFR,NF-κBp65,IkappaBalpha(IκBα)and Zonula Occludens-1(ZO-1)in gastric tissues were determined by real-time reverse transcription polymerase chain reaction,and the efficacy was observed.Then,plasma metabolomic analysis was performed by UPLC-MS/MS to screen the specific potential biomarkers,metabolic pathways and to explore the possible mechani-sms.RESULTS 48 chemical constituents were identified.Many of them have strong pharmacological activity,the results also revealed that A.japonica significantly improved the pathological damage of gastric tissues,increased the expression levels of IL-10,IκBαrelated to anti-inflammatory factors,decreased the expression levels of IL-1β,NF-κB,NF-κBp65,related to proinflammatory factors,restored the levels of factors about EGF,EGFR,ZO-1 associated with ulcer healing and the levels of factors about NKA associated with energy metabolism.Metabolomic analysis identified 10 potential differential metabolites and enriched 7 related metabolic pathways.CONCLUSION These findings contribute to the understanding of the potential mechanism of A.japonica to improve acetic acidinduced GU,and will be of great importance for the development and clinical application of natural drugs related to A.japonica.
