Acorn is a promising starch-rich resource in fermentation industry,while it contains inevitable tannins that have significantly bio-inhibition.In view of tannins as anti-nutritional factors that are easy to complex wi...Acorn is a promising starch-rich resource in fermentation industry,while it contains inevitable tannins that have significantly bio-inhibition.In view of tannins as anti-nutritional factors that are easy to complex with proteins,a regulatory strategy for in-situ separation of tannins was established to realize the effective valorization of acorn kernel starch.In this strategy,1.14 wt%of milk protein as cheap sacrificial proteins was employed to complex with tannins,thereby successfully precipitating soluble tannins in acorn starch in-situ and regulating the concentration to 3%.As results,this in-situ separation approach could significantly alleviate the inhibitory effect of tannins on the enzymolysis process,resulting in a remarkably increase in enzymolysis yield from 75.8%to 89.4%.Additionally,3%tannins had bi-direction regulatory effect on the subsequent bioconversion of glucose by Gluconobacter oxydans,which could effectively mitigate glucose consumption due to endogenous metabolic processes,thereby substantially enhancing economic competitiveness.Under the sacrifice of 1.14%milk protein,273.3 g 2-ketogluconic acid and 104.3 g 5-ketogluconic acid were obtained from 1000 g tannin,with a yield of 77.9%(based on starch)and 92.1%(based on glucose derived from hydrolysis).The regulation strategy accelerated the process of acorns replacing traditional food resources,and was of significance for the economic prosperity impoverished mountainous and forest areas.展开更多
基金supported by National Natural Science Foundation of China(2240080901,32171730)National Key R&D Program of China(2022YFD1300903)+2 种基金China Postdoctoral Science Foundation(2024M751425)Chinese Postdoctoral Fellowship Program of CPSF(GZB20230306)Postgraduate Research&Practice Innovation Program of Jiangsu Province(KYCX24_1280).
文摘Acorn is a promising starch-rich resource in fermentation industry,while it contains inevitable tannins that have significantly bio-inhibition.In view of tannins as anti-nutritional factors that are easy to complex with proteins,a regulatory strategy for in-situ separation of tannins was established to realize the effective valorization of acorn kernel starch.In this strategy,1.14 wt%of milk protein as cheap sacrificial proteins was employed to complex with tannins,thereby successfully precipitating soluble tannins in acorn starch in-situ and regulating the concentration to 3%.As results,this in-situ separation approach could significantly alleviate the inhibitory effect of tannins on the enzymolysis process,resulting in a remarkably increase in enzymolysis yield from 75.8%to 89.4%.Additionally,3%tannins had bi-direction regulatory effect on the subsequent bioconversion of glucose by Gluconobacter oxydans,which could effectively mitigate glucose consumption due to endogenous metabolic processes,thereby substantially enhancing economic competitiveness.Under the sacrifice of 1.14%milk protein,273.3 g 2-ketogluconic acid and 104.3 g 5-ketogluconic acid were obtained from 1000 g tannin,with a yield of 77.9%(based on starch)and 92.1%(based on glucose derived from hydrolysis).The regulation strategy accelerated the process of acorns replacing traditional food resources,and was of significance for the economic prosperity impoverished mountainous and forest areas.
