VR音频(Spatial Audio)涉及到声源和声场这两个概念以及头部相关传输函数HRTF(head-related transfer function)等技术手段,而最基本的元素是声音对象。本文探索以声音对象制作需求为出发点,以自定义虚拟场景为例,利用Kontakt音频采样...VR音频(Spatial Audio)涉及到声源和声场这两个概念以及头部相关传输函数HRTF(head-related transfer function)等技术手段,而最基本的元素是声音对象。本文探索以声音对象制作需求为出发点,以自定义虚拟场景为例,利用Kontakt音频采样器强大的脚本引擎,通过脚本代码的编辑运行,设计与制作一款特定的虚拟乐器。应用于VR音频前期制作中声音对象的同步录制,是计算机音频采样技术的一种应用尝试。展开更多
Objective To construct and identify the Gpx1-Klk1 vector which contains kidney-specific promoter (Ksp-cadherin). Methods Through PCR amplification, the human Gpx1, Klk1, and Ksp-cadherin cDNA were obtained by taking G...Objective To construct and identify the Gpx1-Klk1 vector which contains kidney-specific promoter (Ksp-cadherin). Methods Through PCR amplification, the human Gpx1, Klk1, and Ksp-cadherin cDNA were obtained by taking Gpx1 cDNA, Klk1 cDNA, and Ksp-cadherin BAC as templates. After being testified, the PCR products were inserted into the expressive vector pIRES-EGFP step-by-step to produce a recombinant vector Ksp-cadherin-Gpx1-Klk1. This vector was examined by restriction enzyme digestion and sequence analysis. Results The recombinant expressive vector Ksp-cadherin-Gpx1-Klk1 was successfully constructed. Conclusion The construction of the recombinant vector Ksp-cadherin-Gpx1-Klk1 laid foundations for investigations in establishing transgenic animal models, the over-expression of Gpx1 and Klk1 in mammal kidney, and gene therapy for ischemia-reperfusion injury during kidney transplantation.展开更多
文摘VR音频(Spatial Audio)涉及到声源和声场这两个概念以及头部相关传输函数HRTF(head-related transfer function)等技术手段,而最基本的元素是声音对象。本文探索以声音对象制作需求为出发点,以自定义虚拟场景为例,利用Kontakt音频采样器强大的脚本引擎,通过脚本代码的编辑运行,设计与制作一款特定的虚拟乐器。应用于VR音频前期制作中声音对象的同步录制,是计算机音频采样技术的一种应用尝试。
基金supported by the National Natural Science Foundation of China (No.30471640)
文摘Objective To construct and identify the Gpx1-Klk1 vector which contains kidney-specific promoter (Ksp-cadherin). Methods Through PCR amplification, the human Gpx1, Klk1, and Ksp-cadherin cDNA were obtained by taking Gpx1 cDNA, Klk1 cDNA, and Ksp-cadherin BAC as templates. After being testified, the PCR products were inserted into the expressive vector pIRES-EGFP step-by-step to produce a recombinant vector Ksp-cadherin-Gpx1-Klk1. This vector was examined by restriction enzyme digestion and sequence analysis. Results The recombinant expressive vector Ksp-cadherin-Gpx1-Klk1 was successfully constructed. Conclusion The construction of the recombinant vector Ksp-cadherin-Gpx1-Klk1 laid foundations for investigations in establishing transgenic animal models, the over-expression of Gpx1 and Klk1 in mammal kidney, and gene therapy for ischemia-reperfusion injury during kidney transplantation.
