A facile strategy was designed to prepare magnetically layered chalcogenide Fe_(3)O_(4)/KMS-1(FK)composite adsorbents combining the advantages of rapid magnetic separation and satisfactory adsorption performance under...A facile strategy was designed to prepare magnetically layered chalcogenide Fe_(3)O_(4)/KMS-1(FK)composite adsorbents combining the advantages of rapid magnetic separation and satisfactory adsorption performance under vigorous stirring in ethanol solution at room temperature.The dispersion degree of Fe_(3)O_(4)in ethanol was superior to that in water,which gave a better result with the preparation in ethanol.Characterization by SEM-EDS,XRD,magnetic test,N_(2)physical-adsorption and TG-DTG demonstrated formation of FK and its adsorption performance.展开更多
Tannases are a family of esterases that catalyze the hydrolysis of ester and depside bonds of hydrolyzable tannins to release small compounds such as glucose.In this work,tannase production from Bacillus cereus strain...Tannases are a family of esterases that catalyze the hydrolysis of ester and depside bonds of hydrolyzable tannins to release small compounds such as glucose.In this work,tannase production from Bacillus cereus strain KMS3-1 was purified and characterized.The standard methods,such as ammonium sulfate precipitation followed by gel filtration chromatography,were used to purify the enzyme tannase from KMS3-1.With 40%enzyme recovery,the purification process yielded a 1.93-fold increase in specific activity.Analysis using SDS-PAGE confirmed a molecular weight of about 40 kDa.Circular dichroism analysis revealed the secondary structure compositionα-helix(12.03%),indicating a dominant role ofβ-turns(38.4%).A pH of 6.0 and a temperature between 50 and 60℃were ideal for enzyme activity.Tannic acid has an enzyme Km value of 3.0×10^(-3) M and a Vmax of 4.45 U/mL.Surfactants,metal ions,organic solvents,and inhibitors significantly influence the enzyme activity.Cyto-toxicity assays revealed the non-toxic nature of the purified enzyme on Vero cells and rat animal models.These results explore and contribute to a better understanding of tannase enzyme properties and their potential ap-plications in the food and beverage industries.展开更多
Considering the expected thermal equilibrium characterizing the physics at the Planck scale, it is here stated, for the first time, that, as a system, the space-time at the Planck scale must be considered as subject t...Considering the expected thermal equilibrium characterizing the physics at the Planck scale, it is here stated, for the first time, that, as a system, the space-time at the Planck scale must be considered as subject to the Kubo-Martin-Schwinger (KMS) condition. Consequently, in the interior of the KMS strip, i.e. from the scale B = 0 to the scale B = lplanck, the fourth coordinate g44 must be considered as complex, the two real poles being 6 = 0 and B = lplanck. This means that within the limits of the KMS strip, the Lorentzian and the Euclidean metric are in a 'quantum superposition state' (or coupled), this entailing a 'unification' (or coupling) between the topological (Euclidean) and the physical (Lorentzian) states of space-time.展开更多
基金supported by the National Natural Science Foundation of China[No.51708535 and 51278481]the Science and Technique Plan Project of Ningbo City[No.2016C51032]the Industry guide(key)project of Fujian province[No.2016H0041].
文摘A facile strategy was designed to prepare magnetically layered chalcogenide Fe_(3)O_(4)/KMS-1(FK)composite adsorbents combining the advantages of rapid magnetic separation and satisfactory adsorption performance under vigorous stirring in ethanol solution at room temperature.The dispersion degree of Fe_(3)O_(4)in ethanol was superior to that in water,which gave a better result with the preparation in ethanol.Characterization by SEM-EDS,XRD,magnetic test,N_(2)physical-adsorption and TG-DTG demonstrated formation of FK and its adsorption performance.
基金the Researchers Supporting Project(no.RSP2024R191),King Saud University,Riyadh,Saudi Arabia.
文摘Tannases are a family of esterases that catalyze the hydrolysis of ester and depside bonds of hydrolyzable tannins to release small compounds such as glucose.In this work,tannase production from Bacillus cereus strain KMS3-1 was purified and characterized.The standard methods,such as ammonium sulfate precipitation followed by gel filtration chromatography,were used to purify the enzyme tannase from KMS3-1.With 40%enzyme recovery,the purification process yielded a 1.93-fold increase in specific activity.Analysis using SDS-PAGE confirmed a molecular weight of about 40 kDa.Circular dichroism analysis revealed the secondary structure compositionα-helix(12.03%),indicating a dominant role ofβ-turns(38.4%).A pH of 6.0 and a temperature between 50 and 60℃were ideal for enzyme activity.Tannic acid has an enzyme Km value of 3.0×10^(-3) M and a Vmax of 4.45 U/mL.Surfactants,metal ions,organic solvents,and inhibitors significantly influence the enzyme activity.Cyto-toxicity assays revealed the non-toxic nature of the purified enzyme on Vero cells and rat animal models.These results explore and contribute to a better understanding of tannase enzyme properties and their potential ap-plications in the food and beverage industries.
文摘Considering the expected thermal equilibrium characterizing the physics at the Planck scale, it is here stated, for the first time, that, as a system, the space-time at the Planck scale must be considered as subject to the Kubo-Martin-Schwinger (KMS) condition. Consequently, in the interior of the KMS strip, i.e. from the scale B = 0 to the scale B = lplanck, the fourth coordinate g44 must be considered as complex, the two real poles being 6 = 0 and B = lplanck. This means that within the limits of the KMS strip, the Lorentzian and the Euclidean metric are in a 'quantum superposition state' (or coupled), this entailing a 'unification' (or coupling) between the topological (Euclidean) and the physical (Lorentzian) states of space-time.
