High yield remains the primary objective of peanut breeding.Key yield components,100-pod weight(HPW),100-seed weight(HSW),and shelling percentage(SP),are critical determinants of overall productivity.This study aimed ...High yield remains the primary objective of peanut breeding.Key yield components,100-pod weight(HPW),100-seed weight(HSW),and shelling percentage(SP),are critical determinants of overall productivity.This study aimed to construct a high-density linkage map using resequencing data from recombinant inbred lines(RILs)derived from a cross between‘Silihong'(Arachis hypogaea var.fastigiate)and‘Jinonghei 3'(A.hypogaea var.hypogaea).The resulting map comprised 4,499 bins distributed across 20 chromosomes,spanning a total length of 1,712.32 cM with an average inter-marker distance of 0.38 cM.A total of 46 quantitative trait loci(QTLs)were identified across three environments.Major QTLs,including qHPW5.2,qHPW18.1,qSP7.1,qSP8.1,qSP8.2,qSP18.1,and qSP18.2,explained phenotypic variation(PVE)of 12.04,11.41,16.53,24.17,10.49,10.82,and 29.89%,respectively.Fourteen QTLs detected across multiple environments were considered stable.Notably,one QTL region(qHPW7,qHSW7.1,and qSP7)was associated with all three traits,accounting for PVE values of 8.91,9.04,and 16.53%for HPW,HSW,and SP,respectively.To validate the accuracy of QTL mapping,a genome-wide association study(GWAS)was conducted using the US mini-core collection.Across two environments,115 single-nucleotide polymorphisms(SNPs)were significantly associated with HPW,HSW,and SP in the association panel.Six SNPs were linked to two traits,explaining an average phenotypic variation of 13.84%.Integration of both mapping populations revealed that AX-176802178,detected on chromosome 7 in the association panel and associated with SP,was located within the confidence interval of QTL qSP7 defined by the recombined inbred lines(RIL)population.Furthermore,three KASP markers were developed and validated in peanut landraces and cultivated varieties.These findings provide valuable insights into the genetic architecture underlying HPW,HSW,and SP,and offer useful molecular tools for marker-assisted selection in peanut breeding programs.展开更多
为了提高洋葱雄性不育恢复基因Ms位点的选择效率,加快育种进程,利用AcSKP1等位基因序列上的SNP差异位点,开发了适用于高通量检测的竞争性等位基因特异性PCR(kompetitive allele specific PCR,KASP)标记。结果表明,KASP标记KMs4可以有效...为了提高洋葱雄性不育恢复基因Ms位点的选择效率,加快育种进程,利用AcSKP1等位基因序列上的SNP差异位点,开发了适用于高通量检测的竞争性等位基因特异性PCR(kompetitive allele specific PCR,KASP)标记。结果表明,KASP标记KMs4可以有效地将F_(2)分离群体Ms位点的基因型分为MsMs、Msms、msms三类,并将BC_(1)分离群体Ms位点的基因型分为Msms和msms两类,其分型结果与AcSKP1标记鉴定及表型观察结果完全一致。此外,该标记在不同遗传背景已知基因型的材料中展现出良好的通用性。利用该标记对洋葱开放授粉群体进行育性基因型筛选系统评价,筛选出具有不同育性特征的育种系,为后续杂交选育提供了优质亲本材料。研究表明,KMs4标记是与洋葱雄性不育恢复基因Ms位点紧密连锁的分子标记,能够实现大规模育种材料的高效基因分型。展开更多
基金financially sponsored by the National Natural Science Foundation of China(320720977)the China Agriculture Research System(CARS-13)+4 种基金the Hebei Agriculture Research System,China(HBCT2024040205)the S&T Program of Hebei,China(23567601H)the Peanut Modern Seed Industry Technology Innovation Team in Hebei Province,China(21326316D-2)the State Key Laboratory of North China Crop Improvement and Regulation,China(NCCIR2020RC-2)the Startup Fund of Hebei Agricultural University,China(YJ2020044)。
文摘High yield remains the primary objective of peanut breeding.Key yield components,100-pod weight(HPW),100-seed weight(HSW),and shelling percentage(SP),are critical determinants of overall productivity.This study aimed to construct a high-density linkage map using resequencing data from recombinant inbred lines(RILs)derived from a cross between‘Silihong'(Arachis hypogaea var.fastigiate)and‘Jinonghei 3'(A.hypogaea var.hypogaea).The resulting map comprised 4,499 bins distributed across 20 chromosomes,spanning a total length of 1,712.32 cM with an average inter-marker distance of 0.38 cM.A total of 46 quantitative trait loci(QTLs)were identified across three environments.Major QTLs,including qHPW5.2,qHPW18.1,qSP7.1,qSP8.1,qSP8.2,qSP18.1,and qSP18.2,explained phenotypic variation(PVE)of 12.04,11.41,16.53,24.17,10.49,10.82,and 29.89%,respectively.Fourteen QTLs detected across multiple environments were considered stable.Notably,one QTL region(qHPW7,qHSW7.1,and qSP7)was associated with all three traits,accounting for PVE values of 8.91,9.04,and 16.53%for HPW,HSW,and SP,respectively.To validate the accuracy of QTL mapping,a genome-wide association study(GWAS)was conducted using the US mini-core collection.Across two environments,115 single-nucleotide polymorphisms(SNPs)were significantly associated with HPW,HSW,and SP in the association panel.Six SNPs were linked to two traits,explaining an average phenotypic variation of 13.84%.Integration of both mapping populations revealed that AX-176802178,detected on chromosome 7 in the association panel and associated with SP,was located within the confidence interval of QTL qSP7 defined by the recombined inbred lines(RIL)population.Furthermore,three KASP markers were developed and validated in peanut landraces and cultivated varieties.These findings provide valuable insights into the genetic architecture underlying HPW,HSW,and SP,and offer useful molecular tools for marker-assisted selection in peanut breeding programs.
文摘为了提高洋葱雄性不育恢复基因Ms位点的选择效率,加快育种进程,利用AcSKP1等位基因序列上的SNP差异位点,开发了适用于高通量检测的竞争性等位基因特异性PCR(kompetitive allele specific PCR,KASP)标记。结果表明,KASP标记KMs4可以有效地将F_(2)分离群体Ms位点的基因型分为MsMs、Msms、msms三类,并将BC_(1)分离群体Ms位点的基因型分为Msms和msms两类,其分型结果与AcSKP1标记鉴定及表型观察结果完全一致。此外,该标记在不同遗传背景已知基因型的材料中展现出良好的通用性。利用该标记对洋葱开放授粉群体进行育性基因型筛选系统评价,筛选出具有不同育性特征的育种系,为后续杂交选育提供了优质亲本材料。研究表明,KMs4标记是与洋葱雄性不育恢复基因Ms位点紧密连锁的分子标记,能够实现大规模育种材料的高效基因分型。
