Reactive dyes are the main dyes in printing and dyeing of cellulosic fibers.Reactive dyes fixation rate is a vital indicator to measure the degree of the covalent bond between cellulose and reactive dyes.However,the d...Reactive dyes are the main dyes in printing and dyeing of cellulosic fibers.Reactive dyes fixation rate is a vital indicator to measure the degree of the covalent bond between cellulose and reactive dyes.However,the determination of the fixation rate is tedious and time-consumptive.Based on the theory of reactive dyes dyeing and application of modern computer color matching technology,the relationship between K/S value and the fixation rate with the reactive dyes on cotton fabric was studied.The feasibility of K/S value instead of the traditional washing method for the determination of reactive dyes fixation rate was proved.In this study,the K/S value of the fabric has an excellent linear relationship to the reactive dyes fixation rate obtained by the washing method.The reactive dyes fixation rate can be obtained through the K/S correction value.展开更多
Objective To investigate the effects of electroacupuncture(EA)on the expression and phosphorylation of insulin signal transduction-related proteins in the hypothalamus of insulin resistance(IR)rats.Methods There were ...Objective To investigate the effects of electroacupuncture(EA)on the expression and phosphorylation of insulin signal transduction-related proteins in the hypothalamus of insulin resistance(IR)rats.Methods There were totally seventy-five Wistar rats.Ten rats were randomly assigned to the Normal group(N).The remaining 65 rats were fed a high-fat diet,fifty successfully modeled rats were randomly divided into the Model(M),EA,Sham EA(S+EA),l-leucine(L),and l-leucine+EA(L+EA)groups,with 10 rats in each group.EA was applied at acupoints“Guanyuan(CV 4)”,“Zhongwan(CV 12)”,“Zusanli(ST 36)”,and“Fenglong(ST 40)”,with each session lasting 10 min,three times per week for 8 weeks.The S+EA group received needle insertion to a depth of≤2 mm without electrical stimulation,with the same treatment duration and same acupoint selection.Body weight,fasting blood glucose(FBG),and insulin sensitivity(glucose infusion rate,GIR)were measured.Western blot analysis was used to assess insulin receptor substrate-1(IRS-1),(Protein kinase B)Akt,glycogen synthase kinase-3β(GSK-3β),mechanistic target of rapamycin complex 1(mTORC1),and Ribosomal S6 kinase 1(S6K1),along with their phosphorylated forms.PCR was used to evaluate mRNA expression of IRS-1,Akt,and GSK-3β.Immunofluorescence was used to detect hypothalamic Akt localization.Results(1)Compared to the N group,the M group exhibited increased body weight,FBG,and phosphorylation of GSK-3β,mTORC1,and S6K1,with decreased GIR,IRS-1,Akt phosphorylation,and mRNA expression(P<0.05,P<0.01).(2)Compared to the M group,the EA and S+EA groups showed reduced body weight,FBG,GSK-3β,mTORC1,and S6K1 phosphorylation,with increased GIR,IRS-1,Akt phosphorylation,and mRNA expression(P<0.05,P<0.01).(3)Compared to the EA group,the S+EA group had higher body weight,GSK-3βphosphorylation,and mRNA expression,with reduced p-IRS-1 and p-Akt expression(P<0.05);the L and L+EA groups showed increased GSK-3β,mTORC1,and S6K1 phosphorylation,with decreased GIR,IRS-1,and Akt mRNA expression(P<0.05).(4)Compared to the L+EA group,the L group exhibited higher GSK-3β,mTORC1,and S6K1 phosphorylation,with lower GIR,Akt mRNA,and p-Akt expression(P<0.05,P<0.01).Conclusion EA positively influences body weight,glucose-lipid metabolism,and insulin sensitivity in IR rats,with regulatory effects on central insulin signal transduction-related proteins,potentially linked to its suppression of hypothalamic mTORC1/S6K1 pathway activity.展开更多
文摘Reactive dyes are the main dyes in printing and dyeing of cellulosic fibers.Reactive dyes fixation rate is a vital indicator to measure the degree of the covalent bond between cellulose and reactive dyes.However,the determination of the fixation rate is tedious and time-consumptive.Based on the theory of reactive dyes dyeing and application of modern computer color matching technology,the relationship between K/S value and the fixation rate with the reactive dyes on cotton fabric was studied.The feasibility of K/S value instead of the traditional washing method for the determination of reactive dyes fixation rate was proved.In this study,the K/S value of the fabric has an excellent linear relationship to the reactive dyes fixation rate obtained by the washing method.The reactive dyes fixation rate can be obtained through the K/S correction value.
基金Supported by the Hubei Natural Science Foundation Joint Fund Project:2023AFD139,2023AFD140the "Shizhen Young Talent" Training Program of the College of Acupuncture and Orthopedics,Hubei University of Chinese Medicinethe National Famous Traditional Chinese Medicine Expert Inheritance Studio Construction Project。
文摘Objective To investigate the effects of electroacupuncture(EA)on the expression and phosphorylation of insulin signal transduction-related proteins in the hypothalamus of insulin resistance(IR)rats.Methods There were totally seventy-five Wistar rats.Ten rats were randomly assigned to the Normal group(N).The remaining 65 rats were fed a high-fat diet,fifty successfully modeled rats were randomly divided into the Model(M),EA,Sham EA(S+EA),l-leucine(L),and l-leucine+EA(L+EA)groups,with 10 rats in each group.EA was applied at acupoints“Guanyuan(CV 4)”,“Zhongwan(CV 12)”,“Zusanli(ST 36)”,and“Fenglong(ST 40)”,with each session lasting 10 min,three times per week for 8 weeks.The S+EA group received needle insertion to a depth of≤2 mm without electrical stimulation,with the same treatment duration and same acupoint selection.Body weight,fasting blood glucose(FBG),and insulin sensitivity(glucose infusion rate,GIR)were measured.Western blot analysis was used to assess insulin receptor substrate-1(IRS-1),(Protein kinase B)Akt,glycogen synthase kinase-3β(GSK-3β),mechanistic target of rapamycin complex 1(mTORC1),and Ribosomal S6 kinase 1(S6K1),along with their phosphorylated forms.PCR was used to evaluate mRNA expression of IRS-1,Akt,and GSK-3β.Immunofluorescence was used to detect hypothalamic Akt localization.Results(1)Compared to the N group,the M group exhibited increased body weight,FBG,and phosphorylation of GSK-3β,mTORC1,and S6K1,with decreased GIR,IRS-1,Akt phosphorylation,and mRNA expression(P<0.05,P<0.01).(2)Compared to the M group,the EA and S+EA groups showed reduced body weight,FBG,GSK-3β,mTORC1,and S6K1 phosphorylation,with increased GIR,IRS-1,Akt phosphorylation,and mRNA expression(P<0.05,P<0.01).(3)Compared to the EA group,the S+EA group had higher body weight,GSK-3βphosphorylation,and mRNA expression,with reduced p-IRS-1 and p-Akt expression(P<0.05);the L and L+EA groups showed increased GSK-3β,mTORC1,and S6K1 phosphorylation,with decreased GIR,IRS-1,and Akt mRNA expression(P<0.05).(4)Compared to the L+EA group,the L group exhibited higher GSK-3β,mTORC1,and S6K1 phosphorylation,with lower GIR,Akt mRNA,and p-Akt expression(P<0.05,P<0.01).Conclusion EA positively influences body weight,glucose-lipid metabolism,and insulin sensitivity in IR rats,with regulatory effects on central insulin signal transduction-related proteins,potentially linked to its suppression of hypothalamic mTORC1/S6K1 pathway activity.
文摘依据FFT→优化窗→IFFT思路,突破线性时频变换的窗函数积分性能桎梏,实现高性能优化窗函数的线性时频变换应用,建立新型时频变换算法——K-S变换.对信号x(t)的FFT频谱向量进行频移处理后,与该频移点下Kaiser优化窗的频谱向量进行Hadamard乘积,再将乘积结果进行FFT逆变换(IFFT),构造出K-S变换复时频矩阵,由此获得x(t)的时间-频率-幅值、时间-频率-相位三维信息;给出逆变换的数学推导与局部性质、线性性质和变分辨率特性;0~150 kHz电网的稳态与时变超谐波信号仿真实验表明,K-S变换的时域、频域分辨能力均优于流行的短时傅里叶变换、S变换,具有优良的变分辨率性能;0~40 kHz超谐波信号的实测证明,基于K-S变换的超谐波电压幅值测量绝对误差均小于0.032 3 V.
