Detector and event visualization are crucial components of high-energy physics(HEP)experimental software.Virtual reality(VR)technologies and multimedia development platforms,such as Unity,offer enhanced display effect...Detector and event visualization are crucial components of high-energy physics(HEP)experimental software.Virtual reality(VR)technologies and multimedia development platforms,such as Unity,offer enhanced display effects and flexible extensibility for visualization in HEP experiments.In this study,we present a VR-based method for detector and event displays in the Jiangmen Underground Neutrino Observatory(JUNO)experiment.This method shares the same detector geometry descriptions and event data model as those in the offline software and provides the necessary data conversion interfaces.The VR methodology facilitates an immersive exploration of the virtual environment in JUNO,enabling users to investigate the detector geometry,visualize event data,and tune the detector simulation and event reconstruction algorithms.Additionally,this approach supports applications in data monitoring,physics data analysis,and public outreach initiatives.展开更多
The Taishan Antineutrino Observatory(TAO)is a satellite experiment of the Jiangmen Underground Neutrino Observatory,located near the Taishan nuclear power plant(NPP).The TAO aims to measure the energy spectrum of reac...The Taishan Antineutrino Observatory(TAO)is a satellite experiment of the Jiangmen Underground Neutrino Observatory,located near the Taishan nuclear power plant(NPP).The TAO aims to measure the energy spectrum of reactor antineutrinos with unprecedented precision,which would benefit both reactor neutrino physics and the nuclear database.A detector geometry and event visualization system was developed for the TAO.The software was based on ROOT packages and embedded in the TAO offline software framework.This provided an intuitive tool for visualizing the detector geometry,tuning the reconstruction algorithm,understanding neutrino physics,and monitoring the operation of reactors at NPP.Further applications of the visualization system in the experimental operation of TAO and its future development are discussed.展开更多
A 20-kiloton liquid scintillator detector is under construction at the Jiangmen Underground Neutrino Observatory(JUNO)for several physics purposes.Detecting neutrinos released from nuclear reactors,the sun,supernova b...A 20-kiloton liquid scintillator detector is under construction at the Jiangmen Underground Neutrino Observatory(JUNO)for several physics purposes.Detecting neutrinos released from nuclear reactors,the sun,supernova bursts,and Earth's atmosphere across a wide energy range necessitates efficient reconstruction algorithms.In this study,we introduce a novel method for reconstructing event energy by counting 3-inch photomultiplier tubes(PMTs)with or without signals.The proposed algorithm demonstrated excellent performance in accurate energy reconstruction,validated with electron Monte Carlo samples covering kinetic energies ranging from 10 MeV to 1 GeV.展开更多
It has been hypothesized that under iron stress high ferric chelate reductase (FCR) activity in the absorptive root of plants tolerant to iron_deficiency will be induced and result in subsequent Fe 2+ transport a...It has been hypothesized that under iron stress high ferric chelate reductase (FCR) activity in the absorptive root of plants tolerant to iron_deficiency will be induced and result in subsequent Fe 2+ transport across the plasmalemma. The activity of FCR and expression of FCR gene (FRO2) in Citrus junos Sieb. ex Tanaka tolerant to iron_deficiency and Poncirus trifoliata (L.) Raf. susceptible to iron_deficiency were determined to elucidate the physiological difference which causes the different tolerance of the two citrus rootstocks to iron stress. The activity of FCR was detectable in excised roots and was stimulated about 20_times in C. junos and only about 3_times in P. trifoliata under iron deficiency for four weeks. The FRO2 of Arabidopsis was used as a probe, the tissue print technique was used to ascertain the expression of the FCR gene in C. junos and P. trifoliata under iron stress. High_level transcripts were observed in the absorptive root, young green stem as well as new leaf of C. junos under iron stress for two weeks, and the transcripts were accumulated only slightly in P. trifoliata at the same time. The results showed that the obvious increase of FCR activity was an important reason for the tolerance of C. junos to iron_deficiency, and the regulation of FCR activity seemed to be at the transcriptional level, and the expression of FRO2 occurred in the root, stem and leaf.展开更多
精卵融合是受精过程中最为重要的步骤。目前公认IZUMO1、JUNO和CD9(cluster of differentiation antigen 9)为精卵融合的必需蛋白质,其中IZUMO1与JUNO在精卵识别时会形成复合体。有研究表明IZUMO1、JUNO和CD9主要参与精卵融合最初的黏...精卵融合是受精过程中最为重要的步骤。目前公认IZUMO1、JUNO和CD9(cluster of differentiation antigen 9)为精卵融合的必需蛋白质,其中IZUMO1与JUNO在精卵识别时会形成复合体。有研究表明IZUMO1、JUNO和CD9主要参与精卵融合最初的黏附过程,且它们之间是相互关联发挥作用的。近年来由于X射线晶体学相关技术的发展,IZUMO1-JUNO晶体结构基本被阐明,然而精卵融合的具体分子机制仍未被完全揭示。所以,对哺乳动物精卵融合必需蛋白质IZUMO1-JUNO/CD9的结构、功能和分子机制进行阐述是十分必要的。展开更多
Background:JUNO and IZUMO1 are the first receptor-ligand protein pairs discovered to be essential for spermoocyte fusion;their interaction is indispensable for fertilization.Methods:PCR was used to clone the full-leng...Background:JUNO and IZUMO1 are the first receptor-ligand protein pairs discovered to be essential for spermoocyte fusion;their interaction is indispensable for fertilization.Methods:PCR was used to clone the full-length DNA sequence of the Juno gene in sheep.The single nucleotide polymorphism(SNP)loci of Juno were genotyped by Sequenom MassARRAY®.PCR combined with rapid amplification of cDNA Ends were used to clone the full-length cDNA sequence of Juno and Izumo1.Reverse transcriptase-PCR(RT-PCR)and real time-quantitative-PCR(RT-qPCR)were used to analyze the genes’expression in tissues of sheep,and single cell RNA-seq was used to analyze the genes’expression in oocytes,granulosa cells and follicular theca of polytocous and monotocous Small Tail Han ewes.Bioinformatics was used to analyze advanced structure and phylogeny of JUNO and IZUMO1 proteins.Results:The full-length DNA sequence of the Juno gene in sheep was cloned and nine SNPs were screened.We found a significant association between the g.848253 C>A locus of Juno and litter size of Small Tail Han sheep(P<0.05).The full-length cDNA sequence of Juno and Izumo1 genes from Small Tail Han sheep were obtained.We found a new segment of the Izumo1 CDS consisting of 35 bp,and we confirmed the Izumo1 gene has 9 exons,not 8.RT-qPCR showed that Juno and Izumo1 genes were highly expressed in ovarian and testicular tissues,respectively(P<0.01).Single cell RNA-seq showed Juno was specifically expressed in oocytes,but not in granulosa cells or follicular theca,while Izumo1 displayed little to no expression in all three cell types.There was no difference in expression of the Juno gene in oocyte and ovarian tissue in sheep with different litter sizes,indicating expression of Juno is not related to litter size traits.Bioinformatic analysis revealed the g.848253 C>A locus of Juno results in a nonconservative missense point mutation leading to a change from Phe to Leu at position 219 in the amino acid sequence.Conclusions:For the first time,this study systematically analyzed the expression,structure and function of Juno and Izumo1 genes and their encoded proteins in Small Tail Han sheep,providing the basis for future studies of the regulatory mechanisms of Juno and Izumo1 genes.展开更多
In this study,we present the large photomultiplier tube(PMT)afterpulse measurement results obtained from the Jiangmen underground neutrino observatory(JUNO)experiment.A total of 11 dynode-PMTs(R12860)from the Hamamats...In this study,we present the large photomultiplier tube(PMT)afterpulse measurement results obtained from the Jiangmen underground neutrino observatory(JUNO)experiment.A total of 11 dynode-PMTs(R12860)from the Hamamatsu company(Hamamatsu Photonics K.K.(HPK))and 150 micro-channel plate PMTs(MCP-PMTs,GDB-6201)from the NNVT company(North Night Vision Technology Co.,Ltd.(NNVT))were tested.Subsequently,an afterpulse model was built according to the afterpulse time distribution and the probability of occurrence for these two types of PMTs.The average ratio of the total afterpulse charge with a delay between 0.5μs and 20μs to the primary pulse charge is∼5.7%(13.2%)for the tested MCPPMTs(dynode-PMTs).The JUNO experiment will deploy 20,01220-inch PMTs;this study will benefit detector simulation,event reconstruction,and data analysis regarding the JUNO experiment.展开更多
The Jiangmen Underground Neutrino Observatory(JUNO)is a multi-purpose neutrino experiment.One of the main goals is to determine the neutrino mass ordering by precisely measuring the energy spectrum of reactor antineut...The Jiangmen Underground Neutrino Observatory(JUNO)is a multi-purpose neutrino experiment.One of the main goals is to determine the neutrino mass ordering by precisely measuring the energy spectrum of reactor antineutrinos.For the detection of reactor antineutrinos,cosmogenic backgrounds,such as^(9)Li/^(8)He and fast neutrons induced by cosmic muons,should be rejected carefully by applying muon veto cuts,which require good muon track reconstruction.With a 20-kton liquid scintillator detector,the simulation shows the proportion of muon bundles(muon multiplicity≥2)to be approximately 8%in JUNO,whereas its reconstruction has been rarely discussed in previous experiments.This study proposes an efficient algorithm for muon track reconstruction based on the charge response of a photomultiplier tube array.This is the first reconstruction of muon bundles in a large-volume liquid scintillator detector.In addition,the algorithm shows good performance and potential for reconstruction for both a single muon and double muons(muon multiplicity=2).The spatial resolution of a single-muon reconstruction was20 cm,and the angular resolution was 0.5°.For doublemuon reconstruction,the spatial and angular resolutions could be 30 cm and 1.0°,respectively.Moreover,this paper also discusses muon classification and the veto strategy.展开更多
Aroma touch therapy is widely used in clinical fields for alleviating pain-related symptoms;however, few studies have reported the pain-relief mechanisms. The present study aimed to elucidate the analgesic effects of ...Aroma touch therapy is widely used in clinical fields for alleviating pain-related symptoms;however, few studies have reported the pain-relief mechanisms. The present study aimed to elucidate the analgesic effects of aroma touch therapy with Citrus junos oil based on the quantitative evaluation of deep brain network (DBN) activity using electroencephalogram (EEG) occipital alpha-2 rhythm (10-13 Hz) powers. Experimental investigations were performed with 13 healthy volunteers using the cold pressor task for simulating chronic pain in three different sessions: a baseline session with no therapies, a control session with a touch therapy, and an aroma touch therapy. We have found for the first time that the interviewed pain ratings represented by Numeric Rating Scale (NRS) scores were strongly correlated with a DBN activity index, which was derived from the slow fluctuation components of occipital EEG alpha-2 rhythm powers. The correlation was characterized by a V-shaped curve in the DBN activity index versus the pain rating, i.e., the NRS score, which provided the complete analgesic states (NRS = 0) for some subjects under aroma touch therapy at an appropriate DBN activity index. Such analgesic states were not so strongly correlated with emotional valence. In conclusion, aroma touch therapy may directly modulate DBN activity so that pain-induced outcomes are minimized.展开更多
The morpho-anatomical structure of the aboveground and underground organs of the rare endemic species </span><i><span style="font-family:Verdana;">Iris </span></i><span style...The morpho-anatomical structure of the aboveground and underground organs of the rare endemic species </span><i><span style="font-family:Verdana;">Iris </span></i><span style="font-family:Verdana;">(</span><i><span style="font-family:Verdana;">Juno</span></i><span style="font-family:Verdana;">)</span><i> <span style="font-family:Verdana;">magnifica</span></i><span style="font-family:Verdana;">, growing under natural conditions of the Zeravshan ridge, Samarkand mountains, has been studied for the first time. A comparative analysis of the morpho-anatomical structure of the aboveground and underground organs revealed characteristic diagnostic signs of a microscopic structure. The complex of anatomical features of the aboveground and underground organs of the studied species are species-specific and can be used to solve taxonomic problems of this genus of plants, as well as the Red Book endemic species of </span><span style="font-family:Verdana;">juno</span><span style="font-family:Verdana;"> irises, are of particular interest in connection with the potential for their vegetative reproduction.展开更多
This article involves the study of the psychic remnants commonly known as Archetypes in Sean O'Casey's play Juno and the Paycock. Archetypes are the images or patterns recurring again and again in literature through...This article involves the study of the psychic remnants commonly known as Archetypes in Sean O'Casey's play Juno and the Paycock. Archetypes are the images or patterns recurring again and again in literature through the ages. It also focuses on the minute difference between archetype and archetypal image as discussed by Jung with special reference to the characters and other images in the play beating relevance with the universal archetypes. This article also highlights the play as a connective link in the series of the different pieces of literature via the unique use of the psychic remnants. It emphasises on the different use of those remnants interwoven in the mosaic of the characters and plot of the play.展开更多
基金supported by the National Natural Science Foundation of China(Nos.12175321,W2443004,11975021,11675275,U1932101)Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA10010900)+2 种基金National Key Research and Development Program of China(Nos.2023YFA1606000 and 2020YFA0406400)National College Students Science and Technology Innovation ProjectUndergraduate Base Scientific Research Project of Sun Yat-sen University。
文摘Detector and event visualization are crucial components of high-energy physics(HEP)experimental software.Virtual reality(VR)technologies and multimedia development platforms,such as Unity,offer enhanced display effects and flexible extensibility for visualization in HEP experiments.In this study,we present a VR-based method for detector and event displays in the Jiangmen Underground Neutrino Observatory(JUNO)experiment.This method shares the same detector geometry descriptions and event data model as those in the offline software and provides the necessary data conversion interfaces.The VR methodology facilitates an immersive exploration of the virtual environment in JUNO,enabling users to investigate the detector geometry,visualize event data,and tune the detector simulation and event reconstruction algorithms.Additionally,this approach supports applications in data monitoring,physics data analysis,and public outreach initiatives.
基金supported by the National Natural Science Foundation of China(Nos.12175321,11975021,and 11675275)the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA10010900)。
文摘The Taishan Antineutrino Observatory(TAO)is a satellite experiment of the Jiangmen Underground Neutrino Observatory,located near the Taishan nuclear power plant(NPP).The TAO aims to measure the energy spectrum of reactor antineutrinos with unprecedented precision,which would benefit both reactor neutrino physics and the nuclear database.A detector geometry and event visualization system was developed for the TAO.The software was based on ROOT packages and embedded in the TAO offline software framework.This provided an intuitive tool for visualizing the detector geometry,tuning the reconstruction algorithm,understanding neutrino physics,and monitoring the operation of reactors at NPP.Further applications of the visualization system in the experimental operation of TAO and its future development are discussed.
基金supported by National Key R&D Program of China(No.2023YFA1606103)National Natural Science Foundation of China(Nos.12375105 and 12005044)the Program of Bagui Scholars Program(XF)。
文摘A 20-kiloton liquid scintillator detector is under construction at the Jiangmen Underground Neutrino Observatory(JUNO)for several physics purposes.Detecting neutrinos released from nuclear reactors,the sun,supernova bursts,and Earth's atmosphere across a wide energy range necessitates efficient reconstruction algorithms.In this study,we introduce a novel method for reconstructing event energy by counting 3-inch photomultiplier tubes(PMTs)with or without signals.The proposed algorithm demonstrated excellent performance in accurate energy reconstruction,validated with electron Monte Carlo samples covering kinetic energies ranging from 10 MeV to 1 GeV.
文摘It has been hypothesized that under iron stress high ferric chelate reductase (FCR) activity in the absorptive root of plants tolerant to iron_deficiency will be induced and result in subsequent Fe 2+ transport across the plasmalemma. The activity of FCR and expression of FCR gene (FRO2) in Citrus junos Sieb. ex Tanaka tolerant to iron_deficiency and Poncirus trifoliata (L.) Raf. susceptible to iron_deficiency were determined to elucidate the physiological difference which causes the different tolerance of the two citrus rootstocks to iron stress. The activity of FCR was detectable in excised roots and was stimulated about 20_times in C. junos and only about 3_times in P. trifoliata under iron deficiency for four weeks. The FRO2 of Arabidopsis was used as a probe, the tissue print technique was used to ascertain the expression of the FCR gene in C. junos and P. trifoliata under iron stress. High_level transcripts were observed in the absorptive root, young green stem as well as new leaf of C. junos under iron stress for two weeks, and the transcripts were accumulated only slightly in P. trifoliata at the same time. The results showed that the obvious increase of FCR activity was an important reason for the tolerance of C. junos to iron_deficiency, and the regulation of FCR activity seemed to be at the transcriptional level, and the expression of FRO2 occurred in the root, stem and leaf.
文摘精卵融合是受精过程中最为重要的步骤。目前公认IZUMO1、JUNO和CD9(cluster of differentiation antigen 9)为精卵融合的必需蛋白质,其中IZUMO1与JUNO在精卵识别时会形成复合体。有研究表明IZUMO1、JUNO和CD9主要参与精卵融合最初的黏附过程,且它们之间是相互关联发挥作用的。近年来由于X射线晶体学相关技术的发展,IZUMO1-JUNO晶体结构基本被阐明,然而精卵融合的具体分子机制仍未被完全揭示。所以,对哺乳动物精卵融合必需蛋白质IZUMO1-JUNO/CD9的结构、功能和分子机制进行阐述是十分必要的。
基金This research was funded by National Natural Science Foundation of China,grant number 31501941Central Public-interest Scientific Institution Basal Research Fund,grant number 2018-YWF-YB-1,and 2015ywf-zd-2+1 种基金the Earmarked Fund for China Agriculture Research System,grant number CARS-38the Agricultural Science and Technology Innovation Program of China,grant number ASTIP-IAS13.
文摘Background:JUNO and IZUMO1 are the first receptor-ligand protein pairs discovered to be essential for spermoocyte fusion;their interaction is indispensable for fertilization.Methods:PCR was used to clone the full-length DNA sequence of the Juno gene in sheep.The single nucleotide polymorphism(SNP)loci of Juno were genotyped by Sequenom MassARRAY®.PCR combined with rapid amplification of cDNA Ends were used to clone the full-length cDNA sequence of Juno and Izumo1.Reverse transcriptase-PCR(RT-PCR)and real time-quantitative-PCR(RT-qPCR)were used to analyze the genes’expression in tissues of sheep,and single cell RNA-seq was used to analyze the genes’expression in oocytes,granulosa cells and follicular theca of polytocous and monotocous Small Tail Han ewes.Bioinformatics was used to analyze advanced structure and phylogeny of JUNO and IZUMO1 proteins.Results:The full-length DNA sequence of the Juno gene in sheep was cloned and nine SNPs were screened.We found a significant association between the g.848253 C>A locus of Juno and litter size of Small Tail Han sheep(P<0.05).The full-length cDNA sequence of Juno and Izumo1 genes from Small Tail Han sheep were obtained.We found a new segment of the Izumo1 CDS consisting of 35 bp,and we confirmed the Izumo1 gene has 9 exons,not 8.RT-qPCR showed that Juno and Izumo1 genes were highly expressed in ovarian and testicular tissues,respectively(P<0.01).Single cell RNA-seq showed Juno was specifically expressed in oocytes,but not in granulosa cells or follicular theca,while Izumo1 displayed little to no expression in all three cell types.There was no difference in expression of the Juno gene in oocyte and ovarian tissue in sheep with different litter sizes,indicating expression of Juno is not related to litter size traits.Bioinformatic analysis revealed the g.848253 C>A locus of Juno results in a nonconservative missense point mutation leading to a change from Phe to Leu at position 219 in the amino acid sequence.Conclusions:For the first time,this study systematically analyzed the expression,structure and function of Juno and Izumo1 genes and their encoded proteins in Small Tail Han sheep,providing the basis for future studies of the regulatory mechanisms of Juno and Izumo1 genes.
基金supported by Strategic Priority Research Program of the Chinese Academy of Sciences (No. XDA10011100),Joint Institute of Nuclear Research (JINR),Russia and Lomonosov Moscow State University in Russia,joint Russian Science Foundation (RSF),DFG (Deutsche Forschungsgemeinschaft)National Natural Science Foundation of China (Nos. 12090062 and 12075087)
文摘In this study,we present the large photomultiplier tube(PMT)afterpulse measurement results obtained from the Jiangmen underground neutrino observatory(JUNO)experiment.A total of 11 dynode-PMTs(R12860)from the Hamamatsu company(Hamamatsu Photonics K.K.(HPK))and 150 micro-channel plate PMTs(MCP-PMTs,GDB-6201)from the NNVT company(North Night Vision Technology Co.,Ltd.(NNVT))were tested.Subsequently,an afterpulse model was built according to the afterpulse time distribution and the probability of occurrence for these two types of PMTs.The average ratio of the total afterpulse charge with a delay between 0.5μs and 20μs to the primary pulse charge is∼5.7%(13.2%)for the tested MCPPMTs(dynode-PMTs).The JUNO experiment will deploy 20,01220-inch PMTs;this study will benefit detector simulation,event reconstruction,and data analysis regarding the JUNO experiment.
基金supported by the National Natural Science Foundation of China(Nos.12005044 and 11975258)the Strategic Priority Research Program of the Chinese Academy of Sciences(Nos.XDA10011200 and XDA10010900)。
文摘The Jiangmen Underground Neutrino Observatory(JUNO)is a multi-purpose neutrino experiment.One of the main goals is to determine the neutrino mass ordering by precisely measuring the energy spectrum of reactor antineutrinos.For the detection of reactor antineutrinos,cosmogenic backgrounds,such as^(9)Li/^(8)He and fast neutrons induced by cosmic muons,should be rejected carefully by applying muon veto cuts,which require good muon track reconstruction.With a 20-kton liquid scintillator detector,the simulation shows the proportion of muon bundles(muon multiplicity≥2)to be approximately 8%in JUNO,whereas its reconstruction has been rarely discussed in previous experiments.This study proposes an efficient algorithm for muon track reconstruction based on the charge response of a photomultiplier tube array.This is the first reconstruction of muon bundles in a large-volume liquid scintillator detector.In addition,the algorithm shows good performance and potential for reconstruction for both a single muon and double muons(muon multiplicity=2).The spatial resolution of a single-muon reconstruction was20 cm,and the angular resolution was 0.5°.For doublemuon reconstruction,the spatial and angular resolutions could be 30 cm and 1.0°,respectively.Moreover,this paper also discusses muon classification and the veto strategy.
文摘Aroma touch therapy is widely used in clinical fields for alleviating pain-related symptoms;however, few studies have reported the pain-relief mechanisms. The present study aimed to elucidate the analgesic effects of aroma touch therapy with Citrus junos oil based on the quantitative evaluation of deep brain network (DBN) activity using electroencephalogram (EEG) occipital alpha-2 rhythm (10-13 Hz) powers. Experimental investigations were performed with 13 healthy volunteers using the cold pressor task for simulating chronic pain in three different sessions: a baseline session with no therapies, a control session with a touch therapy, and an aroma touch therapy. We have found for the first time that the interviewed pain ratings represented by Numeric Rating Scale (NRS) scores were strongly correlated with a DBN activity index, which was derived from the slow fluctuation components of occipital EEG alpha-2 rhythm powers. The correlation was characterized by a V-shaped curve in the DBN activity index versus the pain rating, i.e., the NRS score, which provided the complete analgesic states (NRS = 0) for some subjects under aroma touch therapy at an appropriate DBN activity index. Such analgesic states were not so strongly correlated with emotional valence. In conclusion, aroma touch therapy may directly modulate DBN activity so that pain-induced outcomes are minimized.
文摘The morpho-anatomical structure of the aboveground and underground organs of the rare endemic species </span><i><span style="font-family:Verdana;">Iris </span></i><span style="font-family:Verdana;">(</span><i><span style="font-family:Verdana;">Juno</span></i><span style="font-family:Verdana;">)</span><i> <span style="font-family:Verdana;">magnifica</span></i><span style="font-family:Verdana;">, growing under natural conditions of the Zeravshan ridge, Samarkand mountains, has been studied for the first time. A comparative analysis of the morpho-anatomical structure of the aboveground and underground organs revealed characteristic diagnostic signs of a microscopic structure. The complex of anatomical features of the aboveground and underground organs of the studied species are species-specific and can be used to solve taxonomic problems of this genus of plants, as well as the Red Book endemic species of </span><span style="font-family:Verdana;">juno</span><span style="font-family:Verdana;"> irises, are of particular interest in connection with the potential for their vegetative reproduction.
文摘This article involves the study of the psychic remnants commonly known as Archetypes in Sean O'Casey's play Juno and the Paycock. Archetypes are the images or patterns recurring again and again in literature through the ages. It also focuses on the minute difference between archetype and archetypal image as discussed by Jung with special reference to the characters and other images in the play beating relevance with the universal archetypes. This article also highlights the play as a connective link in the series of the different pieces of literature via the unique use of the psychic remnants. It emphasises on the different use of those remnants interwoven in the mosaic of the characters and plot of the play.