[Objective] This study was carried out to determine the induction effect of jasmonic acid（JA）on powdery mildew resistance in wheat,the activation effect on the expressions of plant disease resistance related genes,a...[Objective] This study was carried out to determine the induction effect of jasmonic acid（JA）on powdery mildew resistance in wheat,the activation effect on the expressions of plant disease resistance related genes,and to investigate the relationship between the induced resistance and the gene expression patterns.[Method] Three powdery mildew susceptible cultivars of ＂Chinese Spring＂,＂Pumai 9＂ and ＂Zhoumai 18＂ typically representing different phenotypes in the field were employed.The powdery mildew was assessed by detached leaf assay,and real time quantitative RT-PCR was used to determine the expression patterns of 9 disease resistance related genes of PR1（PR1.1）,PR2（β,1-3 glucanase）,PR3（chitinase）,PR4（wheatwin1）,PR5（thaumatin-like protein）,PR9（TaPERO,peroxidase）,PR10,TaGLP2a（germin-like）and Ta-JA2（jasmonate-induced protein）in leaf of the three cultivars.[Result] MeJA application enhanced the powdery mildew resistances of ＂Chinese Spring＂,＂Pumai 9＂ and ＂Zhoumai 18＂.The induced powdery mildew resistance could be detected from 12 h to 96 h after MeJA treatment,and the peak value was at 24 h.Though there were differences between the three cultivars,MeJA significantly effect on the expressions of the 8 disease resistance related genes except TaGLP2a,and the peak values were at 12 h,24 h or 48 h after treatments.The strongest activation of MeJA was on PR9 and PR1 that their expressions could reach more than 100 times of the untreated samples.MeJA strongly activated PR2、PR4、PR5、PR3、PR10 and Ta-JA2,their expression could reach 10 to 70 times,and there was almost no activation effect on TaGLP2a.The induced powdery mildew resistance positively correlated with the induced expressions of the 8 disease related genes.[Conclusion] The induced powdery mildew resistance positively correlated with the induced expressions of the disease related genes.Jasmonate signalling plays a role in defence against Blumeria graminis f.sp.tritici.and future manipulation of this pathway may improve powdery mildew resistance in wheat.展开更多
通过施加外源Me JA,SA及2种内生菌处理,研究白及组培苗生理及总酚含量的影响。采用组培的方法将种子培养为无菌苗,再对其施加不同处理,观察及测定生理和总酚含量的变化。结果发现:SA各浓度处理下幼苗生长情况均较差,40μmol·L^(-1)...通过施加外源Me JA,SA及2种内生菌处理,研究白及组培苗生理及总酚含量的影响。采用组培的方法将种子培养为无菌苗,再对其施加不同处理,观察及测定生理和总酚含量的变化。结果发现:SA各浓度处理下幼苗生长情况均较差,40μmol·L^(-1)Me JA,50 m L·L^(-1)Hypocrea koningii及10 m L·L^(-1)Trichoderma koningiopsis处理下幼苗生长状况较好;各浓度SA处理下,SOD,POD及CAT活性均较高,Me JA处理下SOD,POD在较高浓度时活性较高,CAT活性则在80μmol·L^(-1)时较高,H.koningii处理下SOD,POD活性均随处理浓度的升高而升高,CAT活性则在1 m L·L^(-1)时较高,T.koningiopsis处理下SOD,POD,CAT活性均随浓度升高先升高后降低,均在10 m L·L^(-1)时较高。4种处理下MDA、可溶性蛋白及脯氨酸含量均有不同程度的升高;60μmol·L^(-1)Me JA处理下幼苗多糖含量较高;40μmol·L^(-1)Me JA,60μmol·L^(-1)SA,1 m L·L^(-1)H.koningii及10 m L·L^(-1),T.koningiopsis处理下总酚含量较高。说明添加一定浓度的外源Me JA,SA及内生菌处理均能在一定程度上提高白及幼苗的抗逆性、提高总酚含量,一定浓度下Me JA及2种内生菌处理还具有促进幼苗生长的作用。展开更多
基金Supported by The Key Project of Science and Technology of HenanProvince(102102110040)Innovation Scientists and the Innovation Fund for Outstanding Scholars of Henan Province(104200510013)~~
文摘[Objective] This study was carried out to determine the induction effect of jasmonic acid（JA）on powdery mildew resistance in wheat,the activation effect on the expressions of plant disease resistance related genes,and to investigate the relationship between the induced resistance and the gene expression patterns.[Method] Three powdery mildew susceptible cultivars of ＂Chinese Spring＂,＂Pumai 9＂ and ＂Zhoumai 18＂ typically representing different phenotypes in the field were employed.The powdery mildew was assessed by detached leaf assay,and real time quantitative RT-PCR was used to determine the expression patterns of 9 disease resistance related genes of PR1（PR1.1）,PR2（β,1-3 glucanase）,PR3（chitinase）,PR4（wheatwin1）,PR5（thaumatin-like protein）,PR9（TaPERO,peroxidase）,PR10,TaGLP2a（germin-like）and Ta-JA2（jasmonate-induced protein）in leaf of the three cultivars.[Result] MeJA application enhanced the powdery mildew resistances of ＂Chinese Spring＂,＂Pumai 9＂ and ＂Zhoumai 18＂.The induced powdery mildew resistance could be detected from 12 h to 96 h after MeJA treatment,and the peak value was at 24 h.Though there were differences between the three cultivars,MeJA significantly effect on the expressions of the 8 disease resistance related genes except TaGLP2a,and the peak values were at 12 h,24 h or 48 h after treatments.The strongest activation of MeJA was on PR9 and PR1 that their expressions could reach more than 100 times of the untreated samples.MeJA strongly activated PR2、PR4、PR5、PR3、PR10 and Ta-JA2,their expression could reach 10 to 70 times,and there was almost no activation effect on TaGLP2a.The induced powdery mildew resistance positively correlated with the induced expressions of the 8 disease related genes.[Conclusion] The induced powdery mildew resistance positively correlated with the induced expressions of the disease related genes.Jasmonate signalling plays a role in defence against Blumeria graminis f.sp.tritici.and future manipulation of this pathway may improve powdery mildew resistance in wheat.
文摘通过施加外源Me JA,SA及2种内生菌处理,研究白及组培苗生理及总酚含量的影响。采用组培的方法将种子培养为无菌苗,再对其施加不同处理,观察及测定生理和总酚含量的变化。结果发现:SA各浓度处理下幼苗生长情况均较差,40μmol·L^(-1)Me JA,50 m L·L^(-1)Hypocrea koningii及10 m L·L^(-1)Trichoderma koningiopsis处理下幼苗生长状况较好;各浓度SA处理下,SOD,POD及CAT活性均较高,Me JA处理下SOD,POD在较高浓度时活性较高,CAT活性则在80μmol·L^(-1)时较高,H.koningii处理下SOD,POD活性均随处理浓度的升高而升高,CAT活性则在1 m L·L^(-1)时较高,T.koningiopsis处理下SOD,POD,CAT活性均随浓度升高先升高后降低,均在10 m L·L^(-1)时较高。4种处理下MDA、可溶性蛋白及脯氨酸含量均有不同程度的升高;60μmol·L^(-1)Me JA处理下幼苗多糖含量较高;40μmol·L^(-1)Me JA,60μmol·L^(-1)SA,1 m L·L^(-1)H.koningii及10 m L·L^(-1),T.koningiopsis处理下总酚含量较高。说明添加一定浓度的外源Me JA,SA及内生菌处理均能在一定程度上提高白及幼苗的抗逆性、提高总酚含量,一定浓度下Me JA及2种内生菌处理还具有促进幼苗生长的作用。
