Lonicera japonica(honeysuckle)is a traditional Chinese medicinal food,in which the main active ingredients are phenolic acids,polysaccharides,flavonoids,and volatile oils.They have various biological activities,includ...Lonicera japonica(honeysuckle)is a traditional Chinese medicinal food,in which the main active ingredients are phenolic acids,polysaccharides,flavonoids,and volatile oils.They have various biological activities,including antiviral,antibacterial,antioxidant,hypoglycemic and lipid-lowering,and anti-inflammatory effects.This review summarizes the health effects and pharmacodynamic mechanisms of L.japonica extracts and the major active ingredients in these extracts,and the structures,metabolic process in vivo,and biotransformation processes of these compounds.In addition,the current status of the development of L.japonica-related functional foods is summarized.The aim is to provide a theoretical basis and reference for the further development and use of the active ingredients in L.japonica as functional foods for disease prevention and treatment.展开更多
Objective To evaluate the protective effect of Lonicerae Japonicae Flos(LJF)extract against doxorubicin(DOX)-induced cardiotoxicity(DIC)and explore the possible mechanisms.Methods Network pharmacology,bioinformatics a...Objective To evaluate the protective effect of Lonicerae Japonicae Flos(LJF)extract against doxorubicin(DOX)-induced cardiotoxicity(DIC)and explore the possible mechanisms.Methods Network pharmacology,bioinformatics analysis and molecular docking were used to predict the targets of the core components of LJF.In a mouse model of DOX-induced myocardial injury,the protective effects of different doses of LJF extract were evaluated and the underlying mechanisms were explored by detecting the changes in mouse myocardial functions,myocardial enzymes,myocardial pathologies,and the expressions of inflammatory factors and pyroptosis-related proteins.Results The 10 core ingredients of LJF showed strong binding to AKT,EGFR,and GSK3β.In the animal experiment,the DOX-treated mice,compared with the sham-treated mice,had significantly decreased cardiac output,stroke volume,left ventricular ejection fraction,left ventricular fraction shorting,elevated serum levels of CK-MB and LDH,increased myocardial expressions of IL-18 and IL-1β,obvious myocardial damage,increased expression levels of NLRP3,caspase-1,GSDMD and GSDMD-N,and reduced expressions of EGFR,p-AKT and p-GSK3βproteins in the myocardial tissues.LJF treatment obviously improved myocardial function,decreased myocardial expressions of IL-18,IL-1β,NLRP3,caspase-1,GSDMD and GSDMD-N proteins,and increased the expressions EGFR,p-AKT and p-GSK3βproteins in DOX-treated mice.Conclusion LJF extract alleviates DOX-induced myocardial injury in mice possibly by reducing myocardial inflammation and pyroptosis via targeting EGFR,AKT and GSK3βto regulate the ErbB signaling pathway.展开更多
The Japanese quail (Coturnix japonica) is a disease-resistant, high-productivity bird. It has few breeding constraints. A phenotypic characterization study of the Japanese quail was carried out in southern Côte d...The Japanese quail (Coturnix japonica) is a disease-resistant, high-productivity bird. It has few breeding constraints. A phenotypic characterization study of the Japanese quail was carried out in southern Côte d’Ivoire to gain a better understanding of this bird. The study involved 298 individuals. The phenotypes were white, black, gray, and red. A total of 11 morphometric parameters were examined. Variables were significantly different between the phenotype and sex groups. The female quail was larger than the male quail. The quail was 20.77 and 4.10 cm long. The head was supported by a 5.89 cm-long neck. The tail was 3.61 cm long. The wing length was 10.42 cm, and the thigh diameter was 2.12 cm. The tarsus was 3.39 cm long and 0.61 cm in diameter. The average quail weight was 297.06 g. The quails in this study exhibit a high degree of morphological variation between individuals in terms of plumage. These results can help develop strategies for developing and genetically improving this short-cycle resource.展开更多
The novel identified receptor,GPR103,now renamed QRFPR(also referred to as SP9155 or AQ27),is the endogenous receptor for the neuropeptide QRFP(also referred to as 26RFa).The distribution pattern,structure,and biologi...The novel identified receptor,GPR103,now renamed QRFPR(also referred to as SP9155 or AQ27),is the endogenous receptor for the neuropeptide QRFP(also referred to as 26RFa).The distribution pattern,structure,and biological actions,such as feeding behavior,bone formation,and hormone secretion of QRFPR have been largely described in chordate species,while no research on QRFPR has been reported in non-chordate species.Here,the first non-chordates QRFP-like peptide receptor gene in the cephalopod Sepiella japonica(Sj_QRFPLR)was identified and characterized.Evidence from multiple alignments,phylogenetic analysis,and in vitro subcellular localization analysis indicated that Sj_QRFPLR is a class A GPCR and it belongs to the QRFPR family.Meanwhile,QRFPR is likely to be structurally conserved in cephalopod species.In situ hybridization and RT-PCR data revealed a widespread distribution pattern of Sj_QRFPLR in multiple function lobes of the female brain and numerous peripheral tissues in both male and female cuttlefish.Subsequently,a food deprivation and refeeding experiment showed that Sj_QRFPLR is likely to stimulate food intake in cuttlefish.Additionally,a possible link between Sj_QRFPLR and immune response was briefly detected in cuttlefish.The results will contribute to our understanding of QRFPR in the cephalopod as well as the peptidergic regulation of the QRFP/QRFPR system in non-chordates.展开更多
In the context of a surging demand for functional foods,this study utilized Sophora japonica L.(SL)and Rosa rugosa Thunb.(RT),which are rich in polyphenols(with flavonoids as the core subclass).High-purity extracts(SL...In the context of a surging demand for functional foods,this study utilized Sophora japonica L.(SL)and Rosa rugosa Thunb.(RT),which are rich in polyphenols(with flavonoids as the core subclass).High-purity extracts(SLE and RTE)were obtained through ethanol reflux extraction and macroporous resin purification,and then formulated with maltodextrin and erythritol to prepare a composite solid beverage.This beverage exhibited excellent antioxidant capabilities.At a concentration of 1 mg/mL,the scavenging rates of DPPH,ABTS,and hydroxyl radicals reached 82.4%,94.6%,and 49.2%,respectively.Network pharmacology indicated that quercetin andβ-sitosterol could modulate lipid metabolism pathways.Moreover,the beverage showed potential for lipid-lowering.Its cholate adsorption capacity was 589.4±2.9 mg/g at pH 7.0,and the IC50 value for pancreatic lipase inhibition was 32.55 mg/mL.However,a 60-day storage stability test revealed that the moisture content approached 5%,likely due to polyphenol-flavonoid reactivity,extending dissolution time to 30.88 s.These changes were attributed to polyphenols(with flavonoids as the core active subclass,and non-flavonoids such as phenolic acids as auxiliary),resulting in color alterations and reduced solubility.This study confirmed the dual functions of the SL-RT beverage in antioxidant and lipid-lowering aspects.Nevertheless,it also pointed out the need to optimize the formula and process to enhance stability,providing an important basis for the development of stable functional beverages.展开更多
Revealing the indica-japonica differentiation in parents of hybridization between indica and japonica rice and their derived lines can provide theoretical and practical bases for the breeding of practical inter-subspe...Revealing the indica-japonica differentiation in parents of hybridization between indica and japonica rice and their derived lines can provide theoretical and practical bases for the breeding of practical inter-subspecific hybrid rice. Using subspeciesspecific molecular markers ILP （intron length polymorphism） and Cheng＇s index, the indica-japonica differentiation was analyzed with special materials including 18 indica-japonica hybrid parents and 39 derived lines, which accumulated different wide compatibility and restoring genes by convergent cross method in 21 years spanning four breeding phases. The indica-japonica differentiation was detected on all tested loci in 57 materials. Among the 18 parental lines, 4 were japonica type, 5 japonicaclinous type, 8 indicaclinous type and one indica type. The japonica proportion indexes in indica restorer lines Minghui 63 and 9308 were 12.50 and 33.33 %, respectively, while that in japonica restorer line C418 was only 31.25%. Among the 39 derived lines from indica-japonica hybridization, one was japonica type, 11 japonicaclinous type, 20 indicaclinous type and 7 indica type. The japonica proportion index in Minghui 502 was only 10.42%. The results of indica and japonica classification by ILP molecular markers and Cheng＇s index were relatively consistent. The correlation coefficient between the japonica proportion index and morphology index was 0.794＊＊, while that between the indica proportion index and morphology index was -0.7662＊＊. ILP markers could be used to accurately detect the proportion of indica/japonica content in the genome of a rice variety. The results of indica-japonica differentiation analysis could make reasonable explanation for that the hybrids obtained from indica-japonica type restorer lines had obvious heterosis. This conclusion would provide important guidance in efficient use of beneficial genes of inter-subspecific hybrid rice.展开更多
The Yangtze River estuary is the main production area of Anguilla japonica in China,as well as the only existing fishery area for adult eels.Japanese eels are distributed in the main rivers and many tributaries from t...The Yangtze River estuary is the main production area of Anguilla japonica in China,as well as the only existing fishery area for adult eels.Japanese eels are distributed in the main rivers and many tributaries from the Yangtze River estuary to the upper Jinsha River,which extend to nearly 3 000 km.However,their migration behaviors remain relatively unknown.We analyzed the biological characteristics of 153 specimens of silver eels collected from the Jingjiang section of Yangtze River（31o30＇N,120o42＇E） between September and November,2008,and tested the sagittal Sr/Ca ratios of 27 specimens.Among the 153 specimens examined,85 were female and 68 were male,which translated to a female-male ratio of 1：0.8.The ages of the female specimens ranged from 3 to 7 a（average 5.52） with an average total length（TL） of（669±80） mm,average body weight（BW） of（555±229）g,average condition factor of 1.77±0.22,and average gonad somatic index（GSI） of 1.32±0.31.The ages of the males ranged from 3 to 5 a（average：4.38） with an average TL of（518±51） mm,average BW of（234±76） g,average condition factor of 1.62±0.18,and average GSI of 0.21±0.11.All biological parameters of females were significantly larger than those of the male specimens（P0.05）.According to the average Sr/Ca ratio（7.99±1.05） ×10-3 of the elver mark of sagitta,17 individuals（62.96%） were river eels and 10 individuals（37.04%） were estuarine eels.Of 16 females,13 individuals（81.25%） were river eels and 3 were estuarine eels,while of 11 males,36.36% were river eels and 63.64% were estuarine eels.The analysis on Sr/Ca ratios for every growth layer group（GLG） indicated there were no significant differences between second-age males and females.However,significant differences were observed between the third-age,fourth-age,and migration-age male and female specimens.This was likely related to the fact that second-age eels of both sexes stay in the same inhabitation waters;however,as they grow older,they move to different areas.展开更多
[Objective] This study aimed to analyze the volatile constituents in Lonicera japonica Thunb. from different origins. [Method] HP-5MS capillary columns were used and column temperature was controlled by a program. MS ...[Objective] This study aimed to analyze the volatile constituents in Lonicera japonica Thunb. from different origins. [Method] HP-5MS capillary columns were used and column temperature was controlled by a program. MS analysis was performed with EI and quadruple mass analyzer. The volatile constituents in L. japonica Thunb. were identified by NIST02 and Wiley275 libraries, and their relative contents were determined with chromatographic peak area normalization method. [Result] According to GC-MS total ion-current chromatograms, 35 volatile constituents were identified in L. japonica Thunb. from Guangxi Zhuang Autonomous Region, mainly including methyl linolenate, n-hexadecanoic acid and ζ-muurolene; 18 volatile constituents were identified in L. japonica Thunb. from Hunan Province, mainly including n-hexadecanoic acid, linoleic acid and α-curcumene. [Conclusion] Main volatile constituents in L. japonica Thunb. from two different origins varied significantly.展开更多
The leaf epidermis of Japanese honeysuckle （Lonicera japonica Thunb.） and Wild Honeysuckle （Lonicera confusa） in the genus of Flos Lonicerae were mainly observed by scanning electron microscopes （SEM） to study t...The leaf epidermis of Japanese honeysuckle （Lonicera japonica Thunb.） and Wild Honeysuckle （Lonicera confusa） in the genus of Flos Lonicerae were mainly observed by scanning electron microscopes （SEM） to study the characteristics of stomata, trichomes and dermal cell, etc.. The results showed that stoma exists only on the lower epidermis and its distribution is irregular, and leaf epidermis consist of epidermis cells, stoma complexes and bushy trichomes including glandular hair and non-glandular hair. On the upper epidermis, anticlinal wall caves in sinuous groove to countercheck the transpiration. Evidences from leaf morphological structures serve as another proof on drought-resistant mechanisms. Some strumaes distributing regularly are hypothesized as oxalic calcium on the lower epidermis under laser scanning confocal microscopy （LSCM） with Fluo-3/AM, which can increase their endurance to drought stress. Therefore, the above characteristics of Flos Lonicerae can reduce the loss of water and make Japanese honeysuckle and Wild Honeysuckle adapt to the droughty environment at Karst area in southwest China. However, there is some difference of the two species. From the SEM （Scanning Electron Microscopy） result, it is shown that on the upper epidermis, some glandular hair regularly present along the midrib of Japanese honeysuckle, but Wild Honeysuckle has no glandular hair on the upper epidermis, which can verify the relationships of Flos Lonicerae species and provide the significance for classification of Flos Lonicerae.展开更多
[Objective] By investigating of change rule rice starch RVA profile properties and the influence of cold tolerance on rice quality,the aim was to provide scientific references to the breeding of new cold-tolerant japo...[Objective] By investigating of change rule rice starch RVA profile properties and the influence of cold tolerance on rice quality,the aim was to provide scientific references to the breeding of new cold-tolerant japonica rice varieties with high quality in the Yunnan plateau.[Method] Four cold-tolerant and five cold-sensitive japonica rice cultivars were grown at three locations with different altitudes in Yunnan plateau to investigate rice starch RVA profile characteristics.[Result] The results showed that with increasing altitude,the setback viscosity in cold-sensitive cultivars increased significantly,while the peak viscosity and breakdown viscosity decreased significantly.However,the peak viscosity and breakdown viscosity in cold-tolerant cultivars initially decreased and then gradually increased with rising altitude,whereas the setback viscosity initially increased and then decreased.[Conclusion] The starch RVA parameters of cold-tolerant cultivars were less sensitive to different environments than those of cold-sensitive cultivars.Cooking and eating quality of cold-tolerant cultivars had relatively stable trends with rising altitude,whereas cooking and eating quality of cold-sensitive cultivars had a trend toward inferior.展开更多
In this study, through vitro culturing anthers of 7 F1 progenies of early Japonica rice in cold region on medium with different Fe2+ contents, it was found that Fe2+ content generated greater impacts on the induction ...In this study, through vitro culturing anthers of 7 F1 progenies of early Japonica rice in cold region on medium with different Fe2+ contents, it was found that Fe2+ content generated greater impacts on the induction rate and green plantlet differentiation. The result demonstrated that if Fe2+ increased from 32 to 40 mg/kg, the induction rate of early Japonica rice anther culture in N6 culture media was more then 1.4 times higher than that in N6 culture media containing 5.6 mg/kg Fe2+. In this concentration range, the induction rate increased with the increase of Fe2+ content, while if the concentration was over this concentration range, the induction rate decreased with the increase of Fe2+, showing single peak distribution. When the Fe2+ was 40 mg/kg in differentiation medium, the differentiation rate decreased dramatically. The green plantlet differentiations of callus which were induced on culture media containing 32-40 mg/kg Fe2+ were different, when they were cultured on MS culture media, and 85.7% materials could increase green plantlet productivity to about 7.8%. Therefore, increasing Fe2+in induction media properly could increase anther culture efficiency of early Japonica rice in cold region.展开更多
The efficiency of 7 treatment methods for breaking dormancy in zoysiagrass ( Zoysia japonica Steud.) seeds was synchronously compared. Two methods in which seeds soaking in 30% NaOH solution for 20 or 80 minutes were ...The efficiency of 7 treatment methods for breaking dormancy in zoysiagrass ( Zoysia japonica Steud.) seeds was synchronously compared. Two methods in which seeds soaking in 30% NaOH solution for 20 or 80 minutes were the optimum for breaking dormancy of seeds due to their simple and easy operation in techniques, economic and rapid effectiveness in performance and high germination rates (77.6% and 88.0% respectively).展开更多
[Objective] The research aimed to map QTL for the sensitivity of the traits related outcrosssing of Japonica rice to exogenous GA3 and provide theoretical basis for breeding and improving the high-sensitivity sterile ...[Objective] The research aimed to map QTL for the sensitivity of the traits related outcrosssing of Japonica rice to exogenous GA3 and provide theoretical basis for breeding and improving the high-sensitivity sterile line.[Method] Taking Japonica rice Xiushui 79 and C bao and their recombinant inbred line population 260 lines as test materials,the sensitivity of 4 traits related outcrossing to Exogenous GA3 and their QTL mapping were studied by using composite interval mapping..[Result]Three QTLs,which cont...展开更多
[Objective] The research aimed to analyze the inhibitory mechanism of Sophora japonica n-hexane extract which significantly inhibited Microcystis aeruginosa in the prior research.[Method] S.japonica n-hexane extract w...[Objective] The research aimed to analyze the inhibitory mechanism of Sophora japonica n-hexane extract which significantly inhibited Microcystis aeruginosa in the prior research.[Method] S.japonica n-hexane extract was used to treat M.aeruginosa.By inspecting chlorophyll a content,protein content,cell membrane permeability and superoxide dismutase（SOD） activity,the inhibitory mechanism of S.japonica n-hexane extract on M.aeruginosa was analyzed initially.[Result] S.japonica n-hexane extract destroyed the cell membrane system of M.aeruginosa,and increased the cell membrane permeability.The contents of chlorophyll a and protein respectively declined to 10% and 50% of that in the control group after cultivated for 7 d,which indicated the photosynthetic reaction system of M.aeruginosa was destroyed.In addition,under the effect of S.japonica n-hexane extract,SOD activity of M.aeruginosa increased in the early period and decreased in the latter period.[Conclusion] The possible inhibitory mechanism of S.japonica n-hexane extract on M.aeruginosa was destroying the cell membrane to increase the membrane permeability;destroying the photosynthetic reaction system to decrease the contents of photosynthetic pigment and protein;making SOD activity showing the phased variation.展开更多
[Objective] This study aimed to investigate the effects of different sowing dates and sites on starch RVA profile characteristics of different ecotypes of japonica rice. [Method] Five different ecotypes of japonica ri...[Objective] This study aimed to investigate the effects of different sowing dates and sites on starch RVA profile characteristics of different ecotypes of japonica rice. [Method] Five different ecotypes of japonica rice were sown at seven different dates in four rice planting regions in Jiangsu Province to analyze the differences in starch RVA profile parameters among different rice varieties. [Result] Among eight parameters of rice starch RVA profile, peak time exhibited no significant differences among different sites, while other seven parameters varied significantly or extremely significantly among different sites, sowing dates and varieties. Specifically, rice variety exerted the most significant effects on rice starch RVA profile parameters. Starch RVA profile characteristics varied significantly among different ecological conditions but exhibited no significant differences among different latitudes. To be specific, in different sites, peak viscosity （PKV）, hot paste viscosity （HPV） and breakdown viscosity （BDV） demonstrated a descending order of Huai＇an 〉 Suzhou 〉 Lianyungang 〉 Yangzhou. Cool paste viscosity （CPV） and consistence viscosity （CSV） were higher in the north than in the south; specifically, CPV and CSV were significantly higher in Lianyungang and Huai＇an than in Yangzhou and Suzhou. Setback viscosity （SBV） showed an increasing trend with increasing latitude; specifically, SBV was significantly lower in Suzhou than in other three sites. Pasting temperature （PAT） and peak time （PET） changed slightly among different latitudes. With the postponing of sowing date, peak viscosity （PKV）, hot paste viscosity （HPV）, cool paste viscosity （CPV） and peak time （PET） showed a decreasing trend, setback viscosity （SBV） and consistence viscosity （CSV） increased, breakdown viscosity （BDV） increased first and then decreased, whereas pasting temperature （PAT） decreased first and then increased. Furthermore, rice starch RVA profile parameters showed the same variation trend with sowing date in different sites; early sowing within suitable sowing dates could effectively improve the parameters of rice starch RVA profile. However, different parameters exerted different variations among different sites and most parameters were higher in the north than in the south, which indicated that starch RVA profile parameters were significantly affected by sowing date in the north of Jiangsu Province. Among eight starch RVA profile parameters, setback viscosity exhibited the maximum variation coefficient, while peak time exhibited the minimum variation coefficient. Among five rice varieties, Nanjing 46 exhibited the highest peak viscosity and breakdown viscosity and the lowest setback viscosity and consistence viscosity, suggesting that Nanjing 46 had the most appropriate starch RVA profile characteristics and the best cooking and eating quality. Lianjing 4 exhibited relatively high peak viscosity and breakdown viscosity, and relatively low setback viscosity and consistence viscosity, suggesting that Lianjing 4 had good starch RVA profile characteristics. Different sowing dates and sites exerted significant effects on starch RVA profiles of different eco- types of japonica rice. Rice starch RVA profile characteristics varied significantly among different sites but exhibited no significant variations among different latitudes. In addition, starch RVA profile characteristics varied significantly among different sowing dates. Therefore, early sowing of the same variety was conducive to improving starch RVA profile characteristics in appropriate planting regions. [Conclusion] This study provided a theoretical basis for producing high-quality rice and improving the eating quality of rice in Jiangsu Province.展开更多
基金supports from the National Natural Science Foundation of China(82130112,U24A20789)Beijing Hospitals Authority Ascent Plan(DFL20190702)Youth Beijing Scholar(2022-051)。
文摘Lonicera japonica(honeysuckle)is a traditional Chinese medicinal food,in which the main active ingredients are phenolic acids,polysaccharides,flavonoids,and volatile oils.They have various biological activities,including antiviral,antibacterial,antioxidant,hypoglycemic and lipid-lowering,and anti-inflammatory effects.This review summarizes the health effects and pharmacodynamic mechanisms of L.japonica extracts and the major active ingredients in these extracts,and the structures,metabolic process in vivo,and biotransformation processes of these compounds.In addition,the current status of the development of L.japonica-related functional foods is summarized.The aim is to provide a theoretical basis and reference for the further development and use of the active ingredients in L.japonica as functional foods for disease prevention and treatment.
文摘Objective To evaluate the protective effect of Lonicerae Japonicae Flos(LJF)extract against doxorubicin(DOX)-induced cardiotoxicity(DIC)and explore the possible mechanisms.Methods Network pharmacology,bioinformatics analysis and molecular docking were used to predict the targets of the core components of LJF.In a mouse model of DOX-induced myocardial injury,the protective effects of different doses of LJF extract were evaluated and the underlying mechanisms were explored by detecting the changes in mouse myocardial functions,myocardial enzymes,myocardial pathologies,and the expressions of inflammatory factors and pyroptosis-related proteins.Results The 10 core ingredients of LJF showed strong binding to AKT,EGFR,and GSK3β.In the animal experiment,the DOX-treated mice,compared with the sham-treated mice,had significantly decreased cardiac output,stroke volume,left ventricular ejection fraction,left ventricular fraction shorting,elevated serum levels of CK-MB and LDH,increased myocardial expressions of IL-18 and IL-1β,obvious myocardial damage,increased expression levels of NLRP3,caspase-1,GSDMD and GSDMD-N,and reduced expressions of EGFR,p-AKT and p-GSK3βproteins in the myocardial tissues.LJF treatment obviously improved myocardial function,decreased myocardial expressions of IL-18,IL-1β,NLRP3,caspase-1,GSDMD and GSDMD-N proteins,and increased the expressions EGFR,p-AKT and p-GSK3βproteins in DOX-treated mice.Conclusion LJF extract alleviates DOX-induced myocardial injury in mice possibly by reducing myocardial inflammation and pyroptosis via targeting EGFR,AKT and GSK3βto regulate the ErbB signaling pathway.
文摘The Japanese quail (Coturnix japonica) is a disease-resistant, high-productivity bird. It has few breeding constraints. A phenotypic characterization study of the Japanese quail was carried out in southern Côte d’Ivoire to gain a better understanding of this bird. The study involved 298 individuals. The phenotypes were white, black, gray, and red. A total of 11 morphometric parameters were examined. Variables were significantly different between the phenotype and sex groups. The female quail was larger than the male quail. The quail was 20.77 and 4.10 cm long. The head was supported by a 5.89 cm-long neck. The tail was 3.61 cm long. The wing length was 10.42 cm, and the thigh diameter was 2.12 cm. The tarsus was 3.39 cm long and 0.61 cm in diameter. The average quail weight was 297.06 g. The quails in this study exhibit a high degree of morphological variation between individuals in terms of plumage. These results can help develop strategies for developing and genetically improving this short-cycle resource.
基金supported by the National Natural Science Foundation of China(No.31872547)the Natural Science Foundation of Zhejiang Province,China(No.LTGN24C190015)the Excellent Postdoctoral Program of Jiangsu Province(No.314865)。
文摘The novel identified receptor,GPR103,now renamed QRFPR(also referred to as SP9155 or AQ27),is the endogenous receptor for the neuropeptide QRFP(also referred to as 26RFa).The distribution pattern,structure,and biological actions,such as feeding behavior,bone formation,and hormone secretion of QRFPR have been largely described in chordate species,while no research on QRFPR has been reported in non-chordate species.Here,the first non-chordates QRFP-like peptide receptor gene in the cephalopod Sepiella japonica(Sj_QRFPLR)was identified and characterized.Evidence from multiple alignments,phylogenetic analysis,and in vitro subcellular localization analysis indicated that Sj_QRFPLR is a class A GPCR and it belongs to the QRFPR family.Meanwhile,QRFPR is likely to be structurally conserved in cephalopod species.In situ hybridization and RT-PCR data revealed a widespread distribution pattern of Sj_QRFPLR in multiple function lobes of the female brain and numerous peripheral tissues in both male and female cuttlefish.Subsequently,a food deprivation and refeeding experiment showed that Sj_QRFPLR is likely to stimulate food intake in cuttlefish.Additionally,a possible link between Sj_QRFPLR and immune response was briefly detected in cuttlefish.The results will contribute to our understanding of QRFPR in the cephalopod as well as the peptidergic regulation of the QRFP/QRFPR system in non-chordates.
文摘In the context of a surging demand for functional foods,this study utilized Sophora japonica L.(SL)and Rosa rugosa Thunb.(RT),which are rich in polyphenols(with flavonoids as the core subclass).High-purity extracts(SLE and RTE)were obtained through ethanol reflux extraction and macroporous resin purification,and then formulated with maltodextrin and erythritol to prepare a composite solid beverage.This beverage exhibited excellent antioxidant capabilities.At a concentration of 1 mg/mL,the scavenging rates of DPPH,ABTS,and hydroxyl radicals reached 82.4%,94.6%,and 49.2%,respectively.Network pharmacology indicated that quercetin andβ-sitosterol could modulate lipid metabolism pathways.Moreover,the beverage showed potential for lipid-lowering.Its cholate adsorption capacity was 589.4±2.9 mg/g at pH 7.0,and the IC50 value for pancreatic lipase inhibition was 32.55 mg/mL.However,a 60-day storage stability test revealed that the moisture content approached 5%,likely due to polyphenol-flavonoid reactivity,extending dissolution time to 30.88 s.These changes were attributed to polyphenols(with flavonoids as the core active subclass,and non-flavonoids such as phenolic acids as auxiliary),resulting in color alterations and reduced solubility.This study confirmed the dual functions of the SL-RT beverage in antioxidant and lipid-lowering aspects.Nevertheless,it also pointed out the need to optimize the formula and process to enhance stability,providing an important basis for the development of stable functional beverages.
基金supported by the National Key Technologies R&D Program of China(2006BAD01A01-3)Important Specialized Science and Technology Item of Fujian Province,China (2004NZ01-4)Important Subject Fund of Science and Technology Department of Fujian Province,China(2007N0070)
文摘Revealing the indica-japonica differentiation in parents of hybridization between indica and japonica rice and their derived lines can provide theoretical and practical bases for the breeding of practical inter-subspecific hybrid rice. Using subspeciesspecific molecular markers ILP （intron length polymorphism） and Cheng＇s index, the indica-japonica differentiation was analyzed with special materials including 18 indica-japonica hybrid parents and 39 derived lines, which accumulated different wide compatibility and restoring genes by convergent cross method in 21 years spanning four breeding phases. The indica-japonica differentiation was detected on all tested loci in 57 materials. Among the 18 parental lines, 4 were japonica type, 5 japonicaclinous type, 8 indicaclinous type and one indica type. The japonica proportion indexes in indica restorer lines Minghui 63 and 9308 were 12.50 and 33.33 %, respectively, while that in japonica restorer line C418 was only 31.25%. Among the 39 derived lines from indica-japonica hybridization, one was japonica type, 11 japonicaclinous type, 20 indicaclinous type and 7 indica type. The japonica proportion index in Minghui 502 was only 10.42%. The results of indica and japonica classification by ILP molecular markers and Cheng＇s index were relatively consistent. The correlation coefficient between the japonica proportion index and morphology index was 0.794＊＊, while that between the indica proportion index and morphology index was -0.7662＊＊. ILP markers could be used to accurately detect the proportion of indica/japonica content in the genome of a rice variety. The results of indica-japonica differentiation analysis could make reasonable explanation for that the hybrids obtained from indica-japonica type restorer lines had obvious heterosis. This conclusion would provide important guidance in efficient use of beneficial genes of inter-subspecific hybrid rice.
基金supported by the Chinese National Science Foundation under the Grant(30771650)the Key Programs of Science and Technology Commission Foundation of Shanghai(08391910200)+3 种基金Special Fund for Agroscientific Research in the Public Interest(201203065)Research Fund for the Doctoral Program of Higher Education of China(20070264001)E-class Programs of Shanghai Municipal Education Commission(E03009)the Key Subject Construction of Shanghai(S30701)
文摘The Yangtze River estuary is the main production area of Anguilla japonica in China,as well as the only existing fishery area for adult eels.Japanese eels are distributed in the main rivers and many tributaries from the Yangtze River estuary to the upper Jinsha River,which extend to nearly 3 000 km.However,their migration behaviors remain relatively unknown.We analyzed the biological characteristics of 153 specimens of silver eels collected from the Jingjiang section of Yangtze River（31o30＇N,120o42＇E） between September and November,2008,and tested the sagittal Sr/Ca ratios of 27 specimens.Among the 153 specimens examined,85 were female and 68 were male,which translated to a female-male ratio of 1：0.8.The ages of the female specimens ranged from 3 to 7 a（average 5.52） with an average total length（TL） of（669±80） mm,average body weight（BW） of（555±229）g,average condition factor of 1.77±0.22,and average gonad somatic index（GSI） of 1.32±0.31.The ages of the males ranged from 3 to 5 a（average：4.38） with an average TL of（518±51） mm,average BW of（234±76） g,average condition factor of 1.62±0.18,and average GSI of 0.21±0.11.All biological parameters of females were significantly larger than those of the male specimens（P0.05）.According to the average Sr/Ca ratio（7.99±1.05） ×10-3 of the elver mark of sagitta,17 individuals（62.96%） were river eels and 10 individuals（37.04%） were estuarine eels.Of 16 females,13 individuals（81.25%） were river eels and 3 were estuarine eels,while of 11 males,36.36% were river eels and 63.64% were estuarine eels.The analysis on Sr/Ca ratios for every growth layer group（GLG） indicated there were no significant differences between second-age males and females.However,significant differences were observed between the third-age,fourth-age,and migration-age male and female specimens.This was likely related to the fact that second-age eels of both sexes stay in the same inhabitation waters;however,as they grow older,they move to different areas.
基金Supported by Natural Science Foundation of Guangxi Zhuang Autonomous Region(2011GXNSFF018006)Special Fund for Bagui Scholar Project~~
文摘[Objective] This study aimed to analyze the volatile constituents in Lonicera japonica Thunb. from different origins. [Method] HP-5MS capillary columns were used and column temperature was controlled by a program. MS analysis was performed with EI and quadruple mass analyzer. The volatile constituents in L. japonica Thunb. were identified by NIST02 and Wiley275 libraries, and their relative contents were determined with chromatographic peak area normalization method. [Result] According to GC-MS total ion-current chromatograms, 35 volatile constituents were identified in L. japonica Thunb. from Guangxi Zhuang Autonomous Region, mainly including methyl linolenate, n-hexadecanoic acid and ζ-muurolene; 18 volatile constituents were identified in L. japonica Thunb. from Hunan Province, mainly including n-hexadecanoic acid, linoleic acid and α-curcumene. [Conclusion] Main volatile constituents in L. japonica Thunb. from two different origins varied significantly.
基金This study was supported by the Ministry of Sciences and Technology of China (No.2005DIB3J067)the National Science Foundation of China (No.40572107, No.40231008, No.40672165 and No.30600074)+2 种基金the Chongqing Science & Technology Commission (No.2005AB7006)the Open Fund and Key Subject of Physical Geog-raphy, Southwest Normal University of China (No.250-411110)the Open Fund of Key Laboratory of Chinese Academy of Geological Sci-ences (No.KL05-20).
文摘The leaf epidermis of Japanese honeysuckle （Lonicera japonica Thunb.） and Wild Honeysuckle （Lonicera confusa） in the genus of Flos Lonicerae were mainly observed by scanning electron microscopes （SEM） to study the characteristics of stomata, trichomes and dermal cell, etc.. The results showed that stoma exists only on the lower epidermis and its distribution is irregular, and leaf epidermis consist of epidermis cells, stoma complexes and bushy trichomes including glandular hair and non-glandular hair. On the upper epidermis, anticlinal wall caves in sinuous groove to countercheck the transpiration. Evidences from leaf morphological structures serve as another proof on drought-resistant mechanisms. Some strumaes distributing regularly are hypothesized as oxalic calcium on the lower epidermis under laser scanning confocal microscopy （LSCM） with Fluo-3/AM, which can increase their endurance to drought stress. Therefore, the above characteristics of Flos Lonicerae can reduce the loss of water and make Japanese honeysuckle and Wild Honeysuckle adapt to the droughty environment at Karst area in southwest China. However, there is some difference of the two species. From the SEM （Scanning Electron Microscopy） result, it is shown that on the upper epidermis, some glandular hair regularly present along the midrib of Japanese honeysuckle, but Wild Honeysuckle has no glandular hair on the upper epidermis, which can verify the relationships of Flos Lonicerae species and provide the significance for classification of Flos Lonicerae.
基金Supported the Key Technologies R&D Program of Yunnan(2010BB002)the National High-tech R & D Program of China(2010AA10Z104 )+2 种基金International Cooperation Programs betweenChina and Korea (YK 2007-2010)Young and Middle-aged Academic Technology Leader Backup Talents Project (2009CI058 )Training Programme for Young and Middle-aged Talents of Technology Innovation by Yunnan (2008PY089)~~
文摘[Objective] By investigating of change rule rice starch RVA profile properties and the influence of cold tolerance on rice quality,the aim was to provide scientific references to the breeding of new cold-tolerant japonica rice varieties with high quality in the Yunnan plateau.[Method] Four cold-tolerant and five cold-sensitive japonica rice cultivars were grown at three locations with different altitudes in Yunnan plateau to investigate rice starch RVA profile characteristics.[Result] The results showed that with increasing altitude,the setback viscosity in cold-sensitive cultivars increased significantly,while the peak viscosity and breakdown viscosity decreased significantly.However,the peak viscosity and breakdown viscosity in cold-tolerant cultivars initially decreased and then gradually increased with rising altitude,whereas the setback viscosity initially increased and then decreased.[Conclusion] The starch RVA parameters of cold-tolerant cultivars were less sensitive to different environments than those of cold-sensitive cultivars.Cooking and eating quality of cold-tolerant cultivars had relatively stable trends with rising altitude,whereas cooking and eating quality of cold-sensitive cultivars had a trend toward inferior.
基金Supported by National Key Technology R&D Program(2006BAD01A01-6)~~
文摘In this study, through vitro culturing anthers of 7 F1 progenies of early Japonica rice in cold region on medium with different Fe2+ contents, it was found that Fe2+ content generated greater impacts on the induction rate and green plantlet differentiation. The result demonstrated that if Fe2+ increased from 32 to 40 mg/kg, the induction rate of early Japonica rice anther culture in N6 culture media was more then 1.4 times higher than that in N6 culture media containing 5.6 mg/kg Fe2+. In this concentration range, the induction rate increased with the increase of Fe2+ content, while if the concentration was over this concentration range, the induction rate decreased with the increase of Fe2+, showing single peak distribution. When the Fe2+ was 40 mg/kg in differentiation medium, the differentiation rate decreased dramatically. The green plantlet differentiations of callus which were induced on culture media containing 32-40 mg/kg Fe2+ were different, when they were cultured on MS culture media, and 85.7% materials could increase green plantlet productivity to about 7.8%. Therefore, increasing Fe2+in induction media properly could increase anther culture efficiency of early Japonica rice in cold region.
文摘The efficiency of 7 treatment methods for breaking dormancy in zoysiagrass ( Zoysia japonica Steud.) seeds was synchronously compared. Two methods in which seeds soaking in 30% NaOH solution for 20 or 80 minutes were the optimum for breaking dormancy of seeds due to their simple and easy operation in techniques, economic and rapid effectiveness in performance and high germination rates (77.6% and 88.0% respectively).
基金Supported by Universities Discipline Innovation and Intellectual Introduction Program Funded Projects(B08025)Key Projects of Basic Scienceand Technology Platform of Ministry of Education(505005)Ministry ofAgriculture Project"948"(2006-G8(4)-31-1)~~
文摘[Objective] The research aimed to map QTL for the sensitivity of the traits related outcrosssing of Japonica rice to exogenous GA3 and provide theoretical basis for breeding and improving the high-sensitivity sterile line.[Method] Taking Japonica rice Xiushui 79 and C bao and their recombinant inbred line population 260 lines as test materials,the sensitivity of 4 traits related outcrossing to Exogenous GA3 and their QTL mapping were studied by using composite interval mapping..[Result]Three QTLs,which cont...
基金Supported by National Natural Science Foundation of China(41076097,41006097,41106113)Science and Technology Research Key Projectof Chinese Ministry of Education(211065)+2 种基金Natural Science Foundation of Jiangsu Province,China(BK2010322)Open Research of Jiangsu Key Laboratory of Environmental Material and Environmental Engineering(K090027,K090025,K090026,K090028)"New Century"Talent Project of Yangzhou University,China~~
文摘[Objective] The research aimed to analyze the inhibitory mechanism of Sophora japonica n-hexane extract which significantly inhibited Microcystis aeruginosa in the prior research.[Method] S.japonica n-hexane extract was used to treat M.aeruginosa.By inspecting chlorophyll a content,protein content,cell membrane permeability and superoxide dismutase（SOD） activity,the inhibitory mechanism of S.japonica n-hexane extract on M.aeruginosa was analyzed initially.[Result] S.japonica n-hexane extract destroyed the cell membrane system of M.aeruginosa,and increased the cell membrane permeability.The contents of chlorophyll a and protein respectively declined to 10% and 50% of that in the control group after cultivated for 7 d,which indicated the photosynthetic reaction system of M.aeruginosa was destroyed.In addition,under the effect of S.japonica n-hexane extract,SOD activity of M.aeruginosa increased in the early period and decreased in the latter period.[Conclusion] The possible inhibitory mechanism of S.japonica n-hexane extract on M.aeruginosa was destroying the cell membrane to increase the membrane permeability;destroying the photosynthetic reaction system to decrease the contents of photosynthetic pigment and protein;making SOD activity showing the phased variation.
文摘[Objective] This study aimed to investigate the effects of different sowing dates and sites on starch RVA profile characteristics of different ecotypes of japonica rice. [Method] Five different ecotypes of japonica rice were sown at seven different dates in four rice planting regions in Jiangsu Province to analyze the differences in starch RVA profile parameters among different rice varieties. [Result] Among eight parameters of rice starch RVA profile, peak time exhibited no significant differences among different sites, while other seven parameters varied significantly or extremely significantly among different sites, sowing dates and varieties. Specifically, rice variety exerted the most significant effects on rice starch RVA profile parameters. Starch RVA profile characteristics varied significantly among different ecological conditions but exhibited no significant differences among different latitudes. To be specific, in different sites, peak viscosity （PKV）, hot paste viscosity （HPV） and breakdown viscosity （BDV） demonstrated a descending order of Huai＇an 〉 Suzhou 〉 Lianyungang 〉 Yangzhou. Cool paste viscosity （CPV） and consistence viscosity （CSV） were higher in the north than in the south; specifically, CPV and CSV were significantly higher in Lianyungang and Huai＇an than in Yangzhou and Suzhou. Setback viscosity （SBV） showed an increasing trend with increasing latitude; specifically, SBV was significantly lower in Suzhou than in other three sites. Pasting temperature （PAT） and peak time （PET） changed slightly among different latitudes. With the postponing of sowing date, peak viscosity （PKV）, hot paste viscosity （HPV）, cool paste viscosity （CPV） and peak time （PET） showed a decreasing trend, setback viscosity （SBV） and consistence viscosity （CSV） increased, breakdown viscosity （BDV） increased first and then decreased, whereas pasting temperature （PAT） decreased first and then increased. Furthermore, rice starch RVA profile parameters showed the same variation trend with sowing date in different sites; early sowing within suitable sowing dates could effectively improve the parameters of rice starch RVA profile. However, different parameters exerted different variations among different sites and most parameters were higher in the north than in the south, which indicated that starch RVA profile parameters were significantly affected by sowing date in the north of Jiangsu Province. Among eight starch RVA profile parameters, setback viscosity exhibited the maximum variation coefficient, while peak time exhibited the minimum variation coefficient. Among five rice varieties, Nanjing 46 exhibited the highest peak viscosity and breakdown viscosity and the lowest setback viscosity and consistence viscosity, suggesting that Nanjing 46 had the most appropriate starch RVA profile characteristics and the best cooking and eating quality. Lianjing 4 exhibited relatively high peak viscosity and breakdown viscosity, and relatively low setback viscosity and consistence viscosity, suggesting that Lianjing 4 had good starch RVA profile characteristics. Different sowing dates and sites exerted significant effects on starch RVA profiles of different eco- types of japonica rice. Rice starch RVA profile characteristics varied significantly among different sites but exhibited no significant variations among different latitudes. In addition, starch RVA profile characteristics varied significantly among different sowing dates. Therefore, early sowing of the same variety was conducive to improving starch RVA profile characteristics in appropriate planting regions. [Conclusion] This study provided a theoretical basis for producing high-quality rice and improving the eating quality of rice in Jiangsu Province.
