目的分析加味凉血消风散对Janus激酶信号转导子与转录激活子(The Janus kinase/signal transducer and activator of tran-scriptions,JAK/STAT)通路中凋亡相关因子蛋白水平的影响,探究凋亡特征和作用微观机制。方法Western Blot检测实...目的分析加味凉血消风散对Janus激酶信号转导子与转录激活子(The Janus kinase/signal transducer and activator of tran-scriptions,JAK/STAT)通路中凋亡相关因子蛋白水平的影响,探究凋亡特征和作用微观机制。方法Western Blot检测实验组治疗前后和对照组血清中JAK3、STAT3、BCL-2、P53表达水平。结果实验组治疗前与对照组比:JAK3、STAT3、BCL-2偏高而P53偏低(P<0.01);实验组治疗后与治疗前比:P53上升而JAK3、STAT3、BCL-2下降(P<0.05)。结论JAK3/STAT3信号通路活化、细胞凋亡受抑可能是发病机制之一;加味凉血消风散可能通过下调JAK3/STAT3通路活化调节P53、BCL-2蛋白水平,促进细胞凋亡,达到治疗血热证寻常型银屑病的目的。展开更多
Neuropathic pain often occurs during chemotherapy with oxaliplatin. AG490 has been shown to exert an antagonistic effect on inflammatory pain, but its effect on oxaliplatin-induced neuropathic pain remains poorly unde...Neuropathic pain often occurs during chemotherapy with oxaliplatin. AG490 has been shown to exert an antagonistic effect on inflammatory pain, but its effect on oxaliplatin-induced neuropathic pain remains poorly understood. This study sought to observe the analgesic effect of AG490 on acute neuropathic pain induced by a single oxaliplatin treatment and to address the possible mechanism. In this study, we estab- lished a model of oxaliplatin-induced acute neuropathic pain by intraperitoneal injection of 6 mg/kg oxaliplatin. On day 2 after injection, models were intraperitoneally injected with i, 5, or 10 mg/kg AG490. Paw withdrawal threshold to mechanical stimuli and tail withdrawal latency to cold stimuli were determined. Western blot assay was performed to detect the expression of spinal phosphorylated signal transducer and activator of transcription 3 （p-STAT3）. Immunohistochemistry was used to determine the immunoreactivity of p-STAT3 and inter- leukin-6. Results demonstrated that paw withdrawal threshold and tail withdrawal latency were significantly increased by the treatment of AG490 in rats. There was no significant difference in the effect among the different doses of AG490. AG490 10 mg/kg decreased the expression of p-STAT3, the immunoreactivity of p-STAT3 and interleukin-6 in spinal cord of acute neuropathic pain rats. These findings confirm that AG490 can attenuate oxaliplatin-induced acute neuropathic pain and is associated with the inhibition in the JAK/STAT3 signaling pathway.展开更多
文摘目的分析加味凉血消风散对Janus激酶信号转导子与转录激活子(The Janus kinase/signal transducer and activator of tran-scriptions,JAK/STAT)通路中凋亡相关因子蛋白水平的影响,探究凋亡特征和作用微观机制。方法Western Blot检测实验组治疗前后和对照组血清中JAK3、STAT3、BCL-2、P53表达水平。结果实验组治疗前与对照组比:JAK3、STAT3、BCL-2偏高而P53偏低(P<0.01);实验组治疗后与治疗前比:P53上升而JAK3、STAT3、BCL-2下降(P<0.05)。结论JAK3/STAT3信号通路活化、细胞凋亡受抑可能是发病机制之一;加味凉血消风散可能通过下调JAK3/STAT3通路活化调节P53、BCL-2蛋白水平,促进细胞凋亡,达到治疗血热证寻常型银屑病的目的。
基金supported by the National Natural Science Foundation of China,No.81671962
文摘Neuropathic pain often occurs during chemotherapy with oxaliplatin. AG490 has been shown to exert an antagonistic effect on inflammatory pain, but its effect on oxaliplatin-induced neuropathic pain remains poorly understood. This study sought to observe the analgesic effect of AG490 on acute neuropathic pain induced by a single oxaliplatin treatment and to address the possible mechanism. In this study, we estab- lished a model of oxaliplatin-induced acute neuropathic pain by intraperitoneal injection of 6 mg/kg oxaliplatin. On day 2 after injection, models were intraperitoneally injected with i, 5, or 10 mg/kg AG490. Paw withdrawal threshold to mechanical stimuli and tail withdrawal latency to cold stimuli were determined. Western blot assay was performed to detect the expression of spinal phosphorylated signal transducer and activator of transcription 3 （p-STAT3）. Immunohistochemistry was used to determine the immunoreactivity of p-STAT3 and inter- leukin-6. Results demonstrated that paw withdrawal threshold and tail withdrawal latency were significantly increased by the treatment of AG490 in rats. There was no significant difference in the effect among the different doses of AG490. AG490 10 mg/kg decreased the expression of p-STAT3, the immunoreactivity of p-STAT3 and interleukin-6 in spinal cord of acute neuropathic pain rats. These findings confirm that AG490 can attenuate oxaliplatin-induced acute neuropathic pain and is associated with the inhibition in the JAK/STAT3 signaling pathway.
