Halobacterium halobium is a typical kind of extremely halophilic bacterium. Combined with the antibiotic resistance assay, the microcalorimetric method was used to study the promoter function of the cloned DNA fragmen...Halobacterium halobium is a typical kind of extremely halophilic bacterium. Combined with the antibiotic resistance assay, the microcalorimetric method was used to study the promoter function of the cloned DNA fragments from Halobacterium halobium J7 plasmid pHH205 in Escherichia coli TG1. The promoter probe vector, plasmid pKK232-8, was used to form the recombinants. The DNA fragment, which is the promoter for the chloramphenicol acetyl transferase （CAT） gene in plasmid pKK232-8, is about 800 bp, and the chloramphenicol resistance level presented by IC50 is about 200 μg·mL^-1, which suggests a high promoter activity. The conclusions show that there probably exist double-function or trinary-function gene promoters in Halobacterium halobium, and Archaea may contain rich genetic resources.展开更多
在2024年11月15-18日举办的2024(第6届)广州国际商用车展上,一汽解放J7创领版牵引车夺得“2025中国年度卡车(Chinese Truck of the Year2025,简称CTOY 2025)”桂冠。这也是一汽解放继J72018年荣获“2019中国年度卡车”称号后,再次斩获...在2024年11月15-18日举办的2024(第6届)广州国际商用车展上,一汽解放J7创领版牵引车夺得“2025中国年度卡车(Chinese Truck of the Year2025,简称CTOY 2025)”桂冠。这也是一汽解放继J72018年荣获“2019中国年度卡车”称号后,再次斩获此殊荣,彰显了其持续领先的技术实力和市场认可度。展开更多
基金Project supported by the National Natural Science Foundation of China （Nos. 30570015, 20373051）, ＂973＂ Project （No. 2004CB719603）, Science Fund for Creative Research Group （No. 20621502 NSFC）, Science Research Foundation of Ministry of Education of China （No. [2006]8IRT0543）, and the Natural Science Foundation of Hubei Province （No. 2005ABC02）.
文摘Halobacterium halobium is a typical kind of extremely halophilic bacterium. Combined with the antibiotic resistance assay, the microcalorimetric method was used to study the promoter function of the cloned DNA fragments from Halobacterium halobium J7 plasmid pHH205 in Escherichia coli TG1. The promoter probe vector, plasmid pKK232-8, was used to form the recombinants. The DNA fragment, which is the promoter for the chloramphenicol acetyl transferase （CAT） gene in plasmid pKK232-8, is about 800 bp, and the chloramphenicol resistance level presented by IC50 is about 200 μg·mL^-1, which suggests a high promoter activity. The conclusions show that there probably exist double-function or trinary-function gene promoters in Halobacterium halobium, and Archaea may contain rich genetic resources.
文摘在2024年11月15-18日举办的2024(第6届)广州国际商用车展上,一汽解放J7创领版牵引车夺得“2025中国年度卡车(Chinese Truck of the Year2025,简称CTOY 2025)”桂冠。这也是一汽解放继J72018年荣获“2019中国年度卡车”称号后,再次斩获此殊荣,彰显了其持续领先的技术实力和市场认可度。
