The death of osteoblasts induced by glucocorticoid(GC)-mediated oxidative stress plays a crucial role in the development of steroid-induced osteonecrosis of the femoral head(SIONFH).Improving bone formation driven by ...The death of osteoblasts induced by glucocorticoid(GC)-mediated oxidative stress plays a crucial role in the development of steroid-induced osteonecrosis of the femoral head(SIONFH).Improving bone formation driven by osteoblasts has shown promising outcomes in the prognosis of SIONFH.Isovitexin has demonstrated antioxidant properties,but its therapeutic effects on GC-induced oxidative stress and SIONFH remain unexplored.In this study,we analyzed clinical samples obtained from SIONFH patients using proteomic and bioinformatic approaches.展开更多
An HPLC method for the determination of isovitexin in rat plasma and different tissues was developed.The separation was achieved on a C_（18）column with a mobile phase consisting of methanol-1% acetum（40：60,v/v）at...An HPLC method for the determination of isovitexin in rat plasma and different tissues was developed.The separation was achieved on a C_（18）column with a mobile phase consisting of methanol-1% acetum（40：60,v/v）at a detection wavelength of 338 nm and a column temperature of 30℃.Rutin was chosen as the internal standard.The linear range of the standard curves was 0.20-128.75μg/mL in the plasma and 0.024-3.09μg/mL in the tissues.The LOQ was 0.19μg/mL in the plasma and 0.024μg/mL in the tissues.The relative recoveries of isovitexin ranged from 93% to 105% in the plasma and 87% to 112% in the tissues.The intra-and inter-day precisions were all below 8%.The pharmacokinetics and tissue distribution of isovitexin in rats were studied with the method.Blood samples were collected at fixed time intervals after the i.v.injection of isovitexin at a dosage of 18.75,3.75 and 0.75 mg/kg;the tissue samples（brain,liver,kidney,heart,lung,spleen and ovary）were obtained at 10,30,and 60 min after the i.v.injection of isovitexin at a dosage of 18.75 mg/kg.The pharmacokinetics of the isovitexin in three different dosages in the rats fit the two-compartment open model.The isovitexin displayed linear dynamics in the dosage range of 0.75-18.75 mg/kg.The mean value of t_（1/2α）was 1.54-1.84 min,and t_（1/2β）was 36.94-46.27 min at the three dosages.The tissue distribution study showed that the sequence of tissue drug concentration from high to low was kidneyliverlung≈ovaryheart≈spleenbrain.展开更多
Mung bean （Vigna radiata L.） is rich in bioactive compounds including D-chiro-inositol （DCI）, vitexin, and isovitexin, which have beneficial effects on patients with diabetes. To find a better source for these val...Mung bean （Vigna radiata L.） is rich in bioactive compounds including D-chiro-inositol （DCI）, vitexin, and isovitexin, which have beneficial effects on patients with diabetes. To find a better source for these valuable chemicals, we have collected 110 varieties of mung bean seed samples and 8 mung bean products to determine the levels of these bioactive compounds. We also measured the DCI content in mung bean sprouts at different germination stages. Content of DCI, vitexin, and isovitexin in all mung bean varieties ranged from 0.43 to 5.79, 0.12 to 3.00, and 0.03 to 1.16 mg g-~, respectively. The varieties of C0001321, C0003522, and C0004485 have the highest DCI, vitexin, and isovitexin contents, respectively. The mung bean products in the market contained relatively lower level of these bioactive components. Contents of DCI, vitexin, and isovitexin in all mung bean products ranged from 0.119 to 0.717, 0 to 0.547, and 0 to 0.923 mg g-~, respectively. During the 112 h of germination test, DCI level steadily increased at first stage and reached the highest level at 80 h of germination （4.79 mg g-~）. These results provide useful information for the selection of suitable varieties and proper germination stages to obtain functional ingredients from mung beans.展开更多
Mung bean （Vigna radiata L.） is rich in D-chiro-inositol （DCI）, vitexin, and isovitexin, which has beneficial effects on antidiabetic and inhibits the formation of advanced glycation end-products. In this study, n...Mung bean （Vigna radiata L.） is rich in D-chiro-inositol （DCI）, vitexin, and isovitexin, which has beneficial effects on antidiabetic and inhibits the formation of advanced glycation end-products. In this study, near-infrared reflectance spectroscopy （NIRS） was used to predict the contents of DCI, vitexin, and isovitexin in mung bean. The spectra data were linearized with those determined by high-performance liquid chromatography （HPLC）. The models for predicting the DCI, vitexin, and isovitexin contents in mung bean were developed using partial least-squares （PLS） algorithm. Cross-validation procedures indicated good correlations between HPLC data and NIRS predictions （R2=0.90 for DCI, R2=0.81 for vitexin, and R2=0.90 for isovitexin）. The predictive contents of DCI, vitexin, and isovitexin ranged from 2.082 to 3.084%, 1.277 to 1.307%, and 0.5998 to 0.6286%, respectively. The results showed that NIRS, a well-established and widely applied technique, could be applied to rapid detection of DCI, vitexin, and isovitexin contents in mung bean.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos:82374475 and 82104883,China).
文摘The death of osteoblasts induced by glucocorticoid(GC)-mediated oxidative stress plays a crucial role in the development of steroid-induced osteonecrosis of the femoral head(SIONFH).Improving bone formation driven by osteoblasts has shown promising outcomes in the prognosis of SIONFH.Isovitexin has demonstrated antioxidant properties,but its therapeutic effects on GC-induced oxidative stress and SIONFH remain unexplored.In this study,we analyzed clinical samples obtained from SIONFH patients using proteomic and bioinformatic approaches.
基金Foundation for Distinguished Young Talents in Higher Education of Guangdong and Foundation for Young Scholars of Guangdong Medical College(Grant No XQ0802)
文摘An HPLC method for the determination of isovitexin in rat plasma and different tissues was developed.The separation was achieved on a C_（18）column with a mobile phase consisting of methanol-1% acetum（40：60,v/v）at a detection wavelength of 338 nm and a column temperature of 30℃.Rutin was chosen as the internal standard.The linear range of the standard curves was 0.20-128.75μg/mL in the plasma and 0.024-3.09μg/mL in the tissues.The LOQ was 0.19μg/mL in the plasma and 0.024μg/mL in the tissues.The relative recoveries of isovitexin ranged from 93% to 105% in the plasma and 87% to 112% in the tissues.The intra-and inter-day precisions were all below 8%.The pharmacokinetics and tissue distribution of isovitexin in rats were studied with the method.Blood samples were collected at fixed time intervals after the i.v.injection of isovitexin at a dosage of 18.75,3.75 and 0.75 mg/kg;the tissue samples（brain,liver,kidney,heart,lung,spleen and ovary）were obtained at 10,30,and 60 min after the i.v.injection of isovitexin at a dosage of 18.75 mg/kg.The pharmacokinetics of the isovitexin in three different dosages in the rats fit the two-compartment open model.The isovitexin displayed linear dynamics in the dosage range of 0.75-18.75 mg/kg.The mean value of t_（1/2α）was 1.54-1.84 min,and t_（1/2β）was 36.94-46.27 min at the three dosages.The tissue distribution study showed that the sequence of tissue drug concentration from high to low was kidneyliverlung≈ovaryheart≈spleenbrain.
基金supported by the Talent Fund(to Dr. Ren Guixing) from the Chinese Academy of Agricultural Sciencesthe Earmarked Fund for Modern Agro-Industry Technology Research System,China(nycytx-018)
文摘Mung bean （Vigna radiata L.） is rich in bioactive compounds including D-chiro-inositol （DCI）, vitexin, and isovitexin, which have beneficial effects on patients with diabetes. To find a better source for these valuable chemicals, we have collected 110 varieties of mung bean seed samples and 8 mung bean products to determine the levels of these bioactive compounds. We also measured the DCI content in mung bean sprouts at different germination stages. Content of DCI, vitexin, and isovitexin in all mung bean varieties ranged from 0.43 to 5.79, 0.12 to 3.00, and 0.03 to 1.16 mg g-~, respectively. The varieties of C0001321, C0003522, and C0004485 have the highest DCI, vitexin, and isovitexin contents, respectively. The mung bean products in the market contained relatively lower level of these bioactive components. Contents of DCI, vitexin, and isovitexin in all mung bean products ranged from 0.119 to 0.717, 0 to 0.547, and 0 to 0.923 mg g-~, respectively. During the 112 h of germination test, DCI level steadily increased at first stage and reached the highest level at 80 h of germination （4.79 mg g-~）. These results provide useful information for the selection of suitable varieties and proper germination stages to obtain functional ingredients from mung beans.
基金supported by the Talent Fund(to Dr. Ren Guixing)from the Chinese Academy of Agricultural Sciencesthe Earmarked Fund for Modern Agro-Industry Technology Research System,China(nycytx-018)
文摘Mung bean （Vigna radiata L.） is rich in D-chiro-inositol （DCI）, vitexin, and isovitexin, which has beneficial effects on antidiabetic and inhibits the formation of advanced glycation end-products. In this study, near-infrared reflectance spectroscopy （NIRS） was used to predict the contents of DCI, vitexin, and isovitexin in mung bean. The spectra data were linearized with those determined by high-performance liquid chromatography （HPLC）. The models for predicting the DCI, vitexin, and isovitexin contents in mung bean were developed using partial least-squares （PLS） algorithm. Cross-validation procedures indicated good correlations between HPLC data and NIRS predictions （R2=0.90 for DCI, R2=0.81 for vitexin, and R2=0.90 for isovitexin）. The predictive contents of DCI, vitexin, and isovitexin ranged from 2.082 to 3.084%, 1.277 to 1.307%, and 0.5998 to 0.6286%, respectively. The results showed that NIRS, a well-established and widely applied technique, could be applied to rapid detection of DCI, vitexin, and isovitexin contents in mung bean.
文摘目的建立不同产地批次大青叶Isatidis Folium的HPLC指纹图谱及指标成分含量测定方法,结合化学模式识别法评价不同产地大青叶质量。方法建立大青叶HPLC指纹图谱,应用中药色谱指纹图谱相似度评价系统(2012.130723版本)、IBM SPSS Statistics 27.0、SIMCA14.1软件,结合聚类分析(hierarchical cluster analysis,HCA)、主成分分析(principal component analysis,PCA)、正交偏最小二乘法-判别分析(orthogonal partial least squares discrimination analysis,OPLS-DA)对不同产地大青叶质量进行评价,根据变量重要性投影(variable importance for projection,VIP)值,对差异性成分异牡荆素、靛玉红进行定量分析,基于含量测定结果建立熵权逼近理想排序法(technique for order preference by similarity to ideal solution,TOPSIS)-灰色关联度(grey relation analysis,GRA)融合模型进一步分析评价。结果建立的大青叶HPLC指纹图谱中,识别共有峰19个,指认峰6个,分别为峰4(丁香酸)、峰9(阿魏酸)、峰12(异牡荆素)、峰16(色胺酮)、峰18(靛蓝)、峰19(靛玉红)。指纹图谱相似度在0.871~0.989;HCA将15批大青叶分为2类;PCA共提取4个成分,累积方差贡献率为91.745%;OPLS-DA筛选出异牡荆素、靛玉红等6个差异性成分。大青叶中异牡荆素、靛玉红的质量分数在0.781~4.385 mg/g、0.134~0.921 mg/g,熵权TOPSIS-GRA融合模型结果显示15批不同产地大青叶样品含量存在差异,编号为S1、S2、S3的样品质量较优。结论建立的HPLC指纹图谱和含量测定结果可为大青叶的质量控制和整体性评价提供参考。
