The effect of Procox? (Bayer, emodepside/toltrazuril suspension for dogs) against natural infections with Isospora spp. was investigated. Two groups were treated either with 0.5 ml suspension/kg of body weight (0.45 m...The effect of Procox? (Bayer, emodepside/toltrazuril suspension for dogs) against natural infections with Isospora spp. was investigated. Two groups were treated either with 0.5 ml suspension/kg of body weight (0.45 mg emodepside and 9 mg toltrazuril/kg of body weight) in the 3rd, 5th and 7th week of life (w.o.l.) (Procox? group;n = 28) or with a control anthelminthic (Dewormed Control group;n = 26). Animals were surveyed weekly from the 3rd w.o.l. by coproscopy and clinical examination. Faecal samples were examined microbiologically from the 4th to the 8th w.o.l. and faecal inflammatory markers canine calprotectin and canine S100A12 were measured in the 8th w.o.l. Specific antibody titres were evaluated in serum samples from five litters before and after vaccination against canine distemper virus and canine parvovirus 2. The prevalence of Isospora-positive animals increased to 67% in the Dewormed Control group (n = 15 puppies from four parasite-positive litters), while in the Procox? group (n = 15 puppies) it was less than 34% with significantly lower excretion (p ? was easily applied and effective;adverse effects did not occur. The level of seroconversion or titre increase upon vaccination was higher in parasite-free animals (91%) compared to Procox?-treated puppies (30%) and the Control animals (10%). Animals from parasite-free litters showed significantly different excretion patterns for haemolytic Escherichia coli and Clostridium perfringens, while there was no difference between Procox?-treated and Control animals. In some animals kept under poor hygienic conditions diarrhoea was noted in association with C. perfringens, E. coli or Salmonella. Concentrations of inflammatory markers in the faeces did not significantly differ between the Procox? and the Control group. Adequate control of parasitic and bacterial infections in suckling puppies requires both antiparasitic treatment and hygiene. Even when parasites do not cause overt effects treatment is recommended in cases with a history of parasite infections.展开更多
参照GenBank收录的Isospora suis 18SrRNA基因全序列(U97523),利用引物分析软件Oligo 6.57和Primer Premier 5.0设计了一对引物(上游引物:5'-tcctgcgagtactcatatgc-3';下游引物:5'-gttcagc-tacgcataccttg-3'),首次扩增...参照GenBank收录的Isospora suis 18SrRNA基因全序列(U97523),利用引物分析软件Oligo 6.57和Primer Premier 5.0设计了一对引物(上游引物:5'-tcctgcgagtactcatatgc-3';下游引物:5'-gttcagc-tacgcataccttg-3'),首次扩增出猪等孢球虫分离株的18SrRNA基因序列,结合GenBank上登录的相关原虫序列,用DNAstar4.0软件比较其同源性,经Clustalx1.81序列比对,Paup4.0、Treeview3.0、Phylip种系发育关系软件分析后,证实该分离株为猪等孢球虫,并且河南不同地区之间的猪等孢球虫没有明显遗传差异。展开更多
对郑州动物园15只孔雀(pavo)的粪便进行了检查,结果发现它们主要感染鸟等孢球虫Isospora Iacazei Labbe,1893;偶见有梅氏艾美缸球虫Eimeriamayurai Bhatia et Pande,1966和曼德勒艾美尔球虫E.mandali Banik et Ray1964。对这些球虫已孢...对郑州动物园15只孔雀(pavo)的粪便进行了检查,结果发现它们主要感染鸟等孢球虫Isospora Iacazei Labbe,1893;偶见有梅氏艾美缸球虫Eimeriamayurai Bhatia et Pande,1966和曼德勒艾美尔球虫E.mandali Banik et Ray1964。对这些球虫已孢子化卵囊作了描述、摄影绘图,并列出了检索表。展开更多
基金financially supported by Bayer Animal Health,Germany.
文摘The effect of Procox? (Bayer, emodepside/toltrazuril suspension for dogs) against natural infections with Isospora spp. was investigated. Two groups were treated either with 0.5 ml suspension/kg of body weight (0.45 mg emodepside and 9 mg toltrazuril/kg of body weight) in the 3rd, 5th and 7th week of life (w.o.l.) (Procox? group;n = 28) or with a control anthelminthic (Dewormed Control group;n = 26). Animals were surveyed weekly from the 3rd w.o.l. by coproscopy and clinical examination. Faecal samples were examined microbiologically from the 4th to the 8th w.o.l. and faecal inflammatory markers canine calprotectin and canine S100A12 were measured in the 8th w.o.l. Specific antibody titres were evaluated in serum samples from five litters before and after vaccination against canine distemper virus and canine parvovirus 2. The prevalence of Isospora-positive animals increased to 67% in the Dewormed Control group (n = 15 puppies from four parasite-positive litters), while in the Procox? group (n = 15 puppies) it was less than 34% with significantly lower excretion (p ? was easily applied and effective;adverse effects did not occur. The level of seroconversion or titre increase upon vaccination was higher in parasite-free animals (91%) compared to Procox?-treated puppies (30%) and the Control animals (10%). Animals from parasite-free litters showed significantly different excretion patterns for haemolytic Escherichia coli and Clostridium perfringens, while there was no difference between Procox?-treated and Control animals. In some animals kept under poor hygienic conditions diarrhoea was noted in association with C. perfringens, E. coli or Salmonella. Concentrations of inflammatory markers in the faeces did not significantly differ between the Procox? and the Control group. Adequate control of parasitic and bacterial infections in suckling puppies requires both antiparasitic treatment and hygiene. Even when parasites do not cause overt effects treatment is recommended in cases with a history of parasite infections.
文摘参照GenBank收录的Isospora suis 18SrRNA基因全序列(U97523),利用引物分析软件Oligo 6.57和Primer Premier 5.0设计了一对引物(上游引物:5'-tcctgcgagtactcatatgc-3';下游引物:5'-gttcagc-tacgcataccttg-3'),首次扩增出猪等孢球虫分离株的18SrRNA基因序列,结合GenBank上登录的相关原虫序列,用DNAstar4.0软件比较其同源性,经Clustalx1.81序列比对,Paup4.0、Treeview3.0、Phylip种系发育关系软件分析后,证实该分离株为猪等孢球虫,并且河南不同地区之间的猪等孢球虫没有明显遗传差异。
文摘对郑州动物园15只孔雀(pavo)的粪便进行了检查,结果发现它们主要感染鸟等孢球虫Isospora Iacazei Labbe,1893;偶见有梅氏艾美缸球虫Eimeriamayurai Bhatia et Pande,1966和曼德勒艾美尔球虫E.mandali Banik et Ray1964。对这些球虫已孢子化卵囊作了描述、摄影绘图,并列出了检索表。
