Minthostachys mollis(Benth)Griseb.is a variety of‘peperina’with high pulegone+methone content but with a higher menthone content.The objective was to evaluate the antioxidant activity(AA)of peperina essential oil(PE...Minthostachys mollis(Benth)Griseb.is a variety of‘peperina’with high pulegone+methone content but with a higher menthone content.The objective was to evaluate the antioxidant activity(AA)of peperina essential oil(PEO)lower in pulegone/MENT(menthone+isomenthone)ratio combined with butylated hydroxytoluene(BHT).PEO was performed by gas chromatography with mass spectrometry(GC-MS).The thermal stability of PEO was determined at 60℃ for 28 days and analysed by GC-MS with SPME.Free Radical Scavening Activity(FRSA-DPPH test)and Total Phenol Content(TPC-analysed with Folin-cicolteau reagent)determined indirect AA.Direct AA was performed with accelerated oxidation of sunflower oil for 28 days at 60℃.Concentrations of 0.02,0.01 and 0.005%w/w(1,2 and 3)were used for the antioxidant BHT(B1,B2 and B3),PEO(P1,P2 and P3)and combinations.Chemical indicators(Conjugated Dienes,Peroxide Value and Anisidine Value),complete oxidation(Total Oxidation Value and Integral Oxidation Value)and volatiles(2-heptenal(Z)and 2,4-Decadienal(E,E))were determined.The pulegone/MENT ratio was 0.59 but the combinations B2P2 and B2P1 allow the use of lower concentration of BHT with higher efficiency.The low pulegone/MENT PEO can be combined with BHT to delay oxidation.展开更多
文摘Minthostachys mollis(Benth)Griseb.is a variety of‘peperina’with high pulegone+methone content but with a higher menthone content.The objective was to evaluate the antioxidant activity(AA)of peperina essential oil(PEO)lower in pulegone/MENT(menthone+isomenthone)ratio combined with butylated hydroxytoluene(BHT).PEO was performed by gas chromatography with mass spectrometry(GC-MS).The thermal stability of PEO was determined at 60℃ for 28 days and analysed by GC-MS with SPME.Free Radical Scavening Activity(FRSA-DPPH test)and Total Phenol Content(TPC-analysed with Folin-cicolteau reagent)determined indirect AA.Direct AA was performed with accelerated oxidation of sunflower oil for 28 days at 60℃.Concentrations of 0.02,0.01 and 0.005%w/w(1,2 and 3)were used for the antioxidant BHT(B1,B2 and B3),PEO(P1,P2 and P3)and combinations.Chemical indicators(Conjugated Dienes,Peroxide Value and Anisidine Value),complete oxidation(Total Oxidation Value and Integral Oxidation Value)and volatiles(2-heptenal(Z)and 2,4-Decadienal(E,E))were determined.The pulegone/MENT ratio was 0.59 but the combinations B2P2 and B2P1 allow the use of lower concentration of BHT with higher efficiency.The low pulegone/MENT PEO can be combined with BHT to delay oxidation.
