Photothermal therapy(PTT)combined with chemotherapy is a promising strategy for tumor treatment.However,its efficacy is often limited by the uncertainty of the irradiation timing of the photothermal agent.Herein,we ra...Photothermal therapy(PTT)combined with chemotherapy is a promising strategy for tumor treatment.However,its efficacy is often limited by the uncertainty of the irradiation timing of the photothermal agent.Herein,we rationally designed a self-assembling peptide-drug conjugate,IR775-Phe-Phe-Lys(CPT)-Lys(Biotin)-OH(IR-FFKK-CPT),which spontaneously self-assembles into fluorescence(FL)-quenched nanoparticles with high photothermal conversion efficiency.After being uptaken by the cancer cells,the nanoparticles are hydrolysed by carboxylesterase and disassembled to release CPT,turning the FL“On”.The“On”FL displays not only the initiation of chemotherapy but also the decline of PTT efficacy.By leveraging the“On”FL as a temporal indicator,we precisely backtrack the optimal cell/tumor irradiation timing to be 4 h/12 h post-incubation/injection in cells/tumors.Subsequent therapeutic studies demonstrated that the timed irradiation on tumor at 12 h post injection significantly maximized tumor treatment outcomes,with average relative tumor volume on day 14 reduced to 13.7%or 10.2%of that in the groups of 6 h or 24 h,respectively.Guided by this timed PTT,IR-FFKK-CPT achieved an excellent tumor growth inhibition rate of 96.2%,significantly outperforming the four positive control groups which showed tumor inhibition rates of 26.3%-34.1%.Our self-regulating theranostic strategy,that synchronizes timed PTT with visualized chemotherapy to maximize tumor treatment,provides people with a promising approach for precise tumor therapy.展开更多
This work aims to explore a procedure to improve biological wastewater treatment efficiency using low inten-sity ultrasound.The aerobic activated sludge from a munici-pal wastewater treatment plant was used as the exp...This work aims to explore a procedure to improve biological wastewater treatment efficiency using low inten-sity ultrasound.The aerobic activated sludge from a munici-pal wastewater treatment plant was used as the experimental material.Oxygen uptake rate(OUR)of the activated sludge(AS)was determined to indicate the changes of AS activity stimulated by ultrasound at 35 kHz for 0-40 min with ultrasonic intensities of 0-1.2 W/cm^(2).The highest OUR was observed at the ultrasonic intensity of 0.3 W/cm^(2) and an irradiation period of 10 min;more than 15%increase was achieved immediately after sonication.More significantly,the AS activity stimulated by ultrasound could last 24 h after sonication,and the AS activity achieved its peak value within 8 h after sonication,or nearly 100%higher than the initial level after sonication.Therefore,to improve the wastewater treatment efficiency of bioreactors,ultrasound with an intensity of 0.3 W/cm^(2) could be employed to irradiate a part of the AS in the bioreactor for 10 min every 8 h.展开更多
基金supported by the National Key Research and Development Program of China(Grant 2023YFF0724100)National Natural Science Foundation of China(Grant 22234002)Natural Science Foundation of Jiangsu Province(Grant BK20232007).
文摘Photothermal therapy(PTT)combined with chemotherapy is a promising strategy for tumor treatment.However,its efficacy is often limited by the uncertainty of the irradiation timing of the photothermal agent.Herein,we rationally designed a self-assembling peptide-drug conjugate,IR775-Phe-Phe-Lys(CPT)-Lys(Biotin)-OH(IR-FFKK-CPT),which spontaneously self-assembles into fluorescence(FL)-quenched nanoparticles with high photothermal conversion efficiency.After being uptaken by the cancer cells,the nanoparticles are hydrolysed by carboxylesterase and disassembled to release CPT,turning the FL“On”.The“On”FL displays not only the initiation of chemotherapy but also the decline of PTT efficacy.By leveraging the“On”FL as a temporal indicator,we precisely backtrack the optimal cell/tumor irradiation timing to be 4 h/12 h post-incubation/injection in cells/tumors.Subsequent therapeutic studies demonstrated that the timed irradiation on tumor at 12 h post injection significantly maximized tumor treatment outcomes,with average relative tumor volume on day 14 reduced to 13.7%or 10.2%of that in the groups of 6 h or 24 h,respectively.Guided by this timed PTT,IR-FFKK-CPT achieved an excellent tumor growth inhibition rate of 96.2%,significantly outperforming the four positive control groups which showed tumor inhibition rates of 26.3%-34.1%.Our self-regulating theranostic strategy,that synchronizes timed PTT with visualized chemotherapy to maximize tumor treatment,provides people with a promising approach for precise tumor therapy.
基金This work was supported by the Ministry of Science and Technology of China(Grant No.2002DFBA0009)。
文摘This work aims to explore a procedure to improve biological wastewater treatment efficiency using low inten-sity ultrasound.The aerobic activated sludge from a munici-pal wastewater treatment plant was used as the experimental material.Oxygen uptake rate(OUR)of the activated sludge(AS)was determined to indicate the changes of AS activity stimulated by ultrasound at 35 kHz for 0-40 min with ultrasonic intensities of 0-1.2 W/cm^(2).The highest OUR was observed at the ultrasonic intensity of 0.3 W/cm^(2) and an irradiation period of 10 min;more than 15%increase was achieved immediately after sonication.More significantly,the AS activity stimulated by ultrasound could last 24 h after sonication,and the AS activity achieved its peak value within 8 h after sonication,or nearly 100%higher than the initial level after sonication.Therefore,to improve the wastewater treatment efficiency of bioreactors,ultrasound with an intensity of 0.3 W/cm^(2) could be employed to irradiate a part of the AS in the bioreactor for 10 min every 8 h.
