Metal-based antimicrobial materials have been extensively studied and applied over decades.While these materials are notably characterized by their superior antibacterial performance and low propensity to induce drug ...Metal-based antimicrobial materials have been extensively studied and applied over decades.While these materials are notably characterized by their superior antibacterial performance and low propensity to induce drug resistance,critical limitations such as inherent cytotoxicity,poor solubility,and instability in aqueous solution remain significant challenges requiring systematic optimization.In this study,we synthesized water-soluble molecular iron-oxo clusters(MIC)with excellent biosafety and stability of aqueous solution.Our findings demonstrate that MIC exhibits marked therapeutic efficacy in cecal ligation and puncture induced sepsis models,a critical validation given sepsis'etiology as a life-threatening infection mediated systemic inflammatory syndrome.MIC combats bacteria by enhancing humoral immune responsiveness.MIC significantly improved the survival rate,reduced bacterial burden,stabilized body temperature,and modulated cytokine profiles in mice with sepsis.Further investigations revealed that MIC promotes B cells proliferation and oxidative phosphorylation,and mitigates mitochondrial damage and apoptosis in B cells,suggesting its role in modulating cellular metabolism.RNA sequencing analysis demonstrated that MIC exerts its effects by influencing key pathways involved in humoral immunity,inflammatory responses,and metabolic adaptation.These findings establish MIC as a novel therapeutic agent for regulating immune responses in sepsis,providing innovative strategies to improve recovery from this life-threatening condition.展开更多
Despite the intrinsic peroxidase-like activity of Prussian blue nanopartides （PBNPs）, their enzyme-mimic mechanism has been scarcely investigated to date. Herein, we probed the catalytic site of PBNPs for the first ...Despite the intrinsic peroxidase-like activity of Prussian blue nanopartides （PBNPs）, their enzyme-mimic mechanism has been scarcely investigated to date. Herein, we probed the catalytic site of PBNPs for the first time, by comparing their peroxidase-like activity with that of a series of Prussian blue analogs （PBAs） in which Fe atoms were replaced by Co, Ni, and Cu. The PBNPs exhibited the highest maximal reaction velocity （1.941 μM·s^-1）, which was at least 13 times higher than that of the PBAs, demonstrating that the peroxidase-like properties of PBNPs could be ascribed to the FeNx （x=4-6） instead of the FeC6 units. Notably, the PBNPs/H2O2 couple also showed much higher oxidizability than .OH radicals produced from the Fenton reaction, implying that a high active Fe（W）=O intermediate might be formed in the FeNx units. This study can thus pave the way for the wider application of PBNPs in biomimetic reactions.展开更多
The tide compound is formed by controlled hydrolytic polymerization in nonaqueous solution of simple oxo-bridged binuclear ferric salts under the presence of carboxylate ligands. The rhombohedral crystalline form of t...The tide compound is formed by controlled hydrolytic polymerization in nonaqueous solution of simple oxo-bridged binuclear ferric salts under the presence of carboxylate ligands. The rhombohedral crystalline form of the cluster with six imidazole molecules, which has crystallographically required C3v symmetry, will be reported. The largest Fe-Fe distance in the undecairon oxo-hydroxo cluster is about 0.68 nm and the cluster has a diameter near 2 nm, which implies that this cluster reaches a nanometer-sized dimension.展开更多
基金the generous supported by the National Key R&D Program of China(No.2023YFC2413100)the National Natural Science Foundation of China(Nos.22322102,21871042 and 21471028)+1 种基金the Fundamental Research Funds for the Central Universities-Excellent Youth Team Program(No.2412023YQ001)the Natural Science Foundation of Jilin Province(Nos.YDZJ202401550ZYTS,20200201083JC)。
文摘Metal-based antimicrobial materials have been extensively studied and applied over decades.While these materials are notably characterized by their superior antibacterial performance and low propensity to induce drug resistance,critical limitations such as inherent cytotoxicity,poor solubility,and instability in aqueous solution remain significant challenges requiring systematic optimization.In this study,we synthesized water-soluble molecular iron-oxo clusters(MIC)with excellent biosafety and stability of aqueous solution.Our findings demonstrate that MIC exhibits marked therapeutic efficacy in cecal ligation and puncture induced sepsis models,a critical validation given sepsis'etiology as a life-threatening infection mediated systemic inflammatory syndrome.MIC combats bacteria by enhancing humoral immune responsiveness.MIC significantly improved the survival rate,reduced bacterial burden,stabilized body temperature,and modulated cytokine profiles in mice with sepsis.Further investigations revealed that MIC promotes B cells proliferation and oxidative phosphorylation,and mitigates mitochondrial damage and apoptosis in B cells,suggesting its role in modulating cellular metabolism.RNA sequencing analysis demonstrated that MIC exerts its effects by influencing key pathways involved in humoral immunity,inflammatory responses,and metabolic adaptation.These findings establish MIC as a novel therapeutic agent for regulating immune responses in sepsis,providing innovative strategies to improve recovery from this life-threatening condition.
文摘Despite the intrinsic peroxidase-like activity of Prussian blue nanopartides （PBNPs）, their enzyme-mimic mechanism has been scarcely investigated to date. Herein, we probed the catalytic site of PBNPs for the first time, by comparing their peroxidase-like activity with that of a series of Prussian blue analogs （PBAs） in which Fe atoms were replaced by Co, Ni, and Cu. The PBNPs exhibited the highest maximal reaction velocity （1.941 μM·s^-1）, which was at least 13 times higher than that of the PBAs, demonstrating that the peroxidase-like properties of PBNPs could be ascribed to the FeNx （x=4-6） instead of the FeC6 units. Notably, the PBNPs/H2O2 couple also showed much higher oxidizability than .OH radicals produced from the Fenton reaction, implying that a high active Fe（W）=O intermediate might be formed in the FeNx units. This study can thus pave the way for the wider application of PBNPs in biomimetic reactions.
基金Project (No. 29871003) Supported by the National Natural Science Foundation of China.
文摘The tide compound is formed by controlled hydrolytic polymerization in nonaqueous solution of simple oxo-bridged binuclear ferric salts under the presence of carboxylate ligands. The rhombohedral crystalline form of the cluster with six imidazole molecules, which has crystallographically required C3v symmetry, will be reported. The largest Fe-Fe distance in the undecairon oxo-hydroxo cluster is about 0.68 nm and the cluster has a diameter near 2 nm, which implies that this cluster reaches a nanometer-sized dimension.
