Studies were performed to determine the extent of nuclear DNA degradation induced by iron, iron-ascorbate, or iron-bleomycin under aerobic conditions in a model system using isolated rat liver nuclei. The effects of f...Studies were performed to determine the extent of nuclear DNA degradation induced by iron, iron-ascorbate, or iron-bleomycin under aerobic conditions in a model system using isolated rat liver nuclei. The effects of five antioxidants (catalase, superoxide dismutase, dimethyl sulfoxide, glutathione and diallyl sulfide) on this oxidative nuclear damage were also investigated. At the 0.05 level for statistical significance, iron induced concentration-dependent DNA degradation, and this effect was enhanced by ascorbate and bleomycin. The antioxidants catalase, dimethyl sulfoxide, and diallyl sulfide significantly reduced the iron-ascorbate-induced DNA damage, whereas superoxide dismutase and dimethyl sulfoxide significantly reduced iron-bleomycin-induced damage. Glutathione significantly increased the iron-bleomycin-induced DNA damage. These results suggest that the reactive oxygen species generated by iron, iron-ascorbate, and iron-bleomycin are responsible for the DNA strand breaks in isolated rat liver nuclei.展开更多
With the support by the National Natural Science Foundation of China and the Ministry of Science and Technology of China,a collaborative study by the research groups led by Prof.Feng Xiaojuan(冯晓娟)from the Institute...With the support by the National Natural Science Foundation of China and the Ministry of Science and Technology of China,a collaborative study by the research groups led by Prof.Feng Xiaojuan(冯晓娟)from the Institute of Botany,Chinese Academy of Sciences and Prof.He Jinsheng(贺金生)from Peking University demonstrates the under-investigated role of iron(Fe)in mediating soil enzyme activity展开更多
文摘Studies were performed to determine the extent of nuclear DNA degradation induced by iron, iron-ascorbate, or iron-bleomycin under aerobic conditions in a model system using isolated rat liver nuclei. The effects of five antioxidants (catalase, superoxide dismutase, dimethyl sulfoxide, glutathione and diallyl sulfide) on this oxidative nuclear damage were also investigated. At the 0.05 level for statistical significance, iron induced concentration-dependent DNA degradation, and this effect was enhanced by ascorbate and bleomycin. The antioxidants catalase, dimethyl sulfoxide, and diallyl sulfide significantly reduced the iron-ascorbate-induced DNA damage, whereas superoxide dismutase and dimethyl sulfoxide significantly reduced iron-bleomycin-induced damage. Glutathione significantly increased the iron-bleomycin-induced DNA damage. These results suggest that the reactive oxygen species generated by iron, iron-ascorbate, and iron-bleomycin are responsible for the DNA strand breaks in isolated rat liver nuclei.
文摘With the support by the National Natural Science Foundation of China and the Ministry of Science and Technology of China,a collaborative study by the research groups led by Prof.Feng Xiaojuan(冯晓娟)from the Institute of Botany,Chinese Academy of Sciences and Prof.He Jinsheng(贺金生)from Peking University demonstrates the under-investigated role of iron(Fe)in mediating soil enzyme activity
